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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... MCF10A human mammary epithelial cells (ATCC) were cultured in 1:1 DMEM/F-12 (Thermo Fisher Scientific, USA) media which consists of 2.50 mM L-Glutamine and 15 mM HEPES buffer ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using a 1:1 ratio of DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher) for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... T cells were activated using a 1:1 ratio of DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher) for 24 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR8-KLK4-KLKP1 (DDK tagged) was recombined with pLenti6/V5-Dest™ (Life Technologies) or pAD/CMV/V5-Dest™ (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-mouse IgG (H+L) antibody (Invitrogen), before being acquired with BD FACSCanto II (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... and c-Myc-tagged N protein together with GFP using Lipofectamine 2000 (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... sections were incubated with the appropriate Alexa 546-tagged secondary antibody (Invitrogen, Carlsbad, CA) for 2 hrs at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged RNF219 and CNOT1 cDNAs were cloned into pCDNA5/FRT-TO vector (Invitrogen). The expression vectors were then transfected into HEK-293 Flp-In-TRex cells and followed by selection with hygromycin to generate stable cell lines ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-tagged GSK3β constructs were cloned into the pDEST™27 vector (ThermoFisher Scientific) as described previously66 ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tagged RASSF5 (GST-RASSF5) sequence was transferred into the pDEST15 expression vector (Thermofisher), and then expressed and purified from BL21-A1cells ...
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bioRxiv - Immunology 2022Quote: ... and RAW 264.7 EROS FLAG-tagged cells were maintained in DMEM medium (21969035, ThermoFisher) containing 10 % FBS (F7524 ...
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bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... was used to generate Flag-tagged Prom1 clones and deletion mutants in pcDNA3.1+ (Invitrogen). The primers used for cloning are listed in Supplementary Table 1.
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bioRxiv - Bioengineering 2023Quote: ... A fluorescently tagged secondary antibody (Goat anti-rabbit, Alexa Fluor 647, Thermo Fisher Scientific) diluted in the same antibody solution was applied for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... HA-tagged scFvs were detected using mouse mAb 2-2.2.14 (ThermoFisher Catalog# P126183, RRID:AB_10978021). The following day sections were washed 4 x 5 min with vehicle ...
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bioRxiv - Developmental Biology 2022Quote: ... and tagged Stat3 were synthesized with linearized plasmids using T7 mMessage mMachine kit (Invitrogen). For overexpression essays ...
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bioRxiv - Cell Biology 2023Quote: ... and His6-tagged VASP (Benz et al., 2009) using Lipofectamine 2000 (Invitrogen, Karlsruhe, Germany). Cells were lysed in buffer A (40 mM HEPES-NaOH ...
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bioRxiv - Developmental Biology 2023Quote: ... The primary labelling was detected using appropriate Alexa-Flour tagged secondary antibody (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... WRN Halo-tagged U2OS cells were seeded in GlutaMAX- supplemented DMEM (Gibco, 10566-016) with 10% FBS and 1% Penicillin/Streptomycin at 6x103 cells per well ...
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bioRxiv - Neuroscience 2023Quote: ... N2A cells were transfected with different HA tagged WDR47 variants using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 6xHis-tagged CapZ was purified as previously described30,49 using HisPur Cobalt resin (Thermo Scientific), and separated from high-molecular weight contaminants by size-exclusion chromatography using a Sephacryl S-200 HR column (Cytiva/GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... and then either directly matured in vitro for 23 hours in groups of 35–70 COCs in 500 μl of maturation medium (31100-027, NaHCO3-buffered M199 [11150059, Gibco BRL] supplemented with 1% (v/v) penicillin-streptomycin [15140122 ...
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bioRxiv - Microbiology 2020Quote: ... high sensitivity kit (ThermoFisher). A standard curve was generated by mixing 10 μL of the eight DNA standards provided (0 to 10 ng/μL ...
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bioRxiv - Biochemistry 2020Quote: ... High Five Cells (Thermofisher) at a density of ∼3×106 cells/ml were infected with baculovirus and grown at 27 °C for 48 56 h before harvesting ...
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bioRxiv - Biochemistry 2020Quote: ... High Five Cells (Thermofisher) at a density of ~3×106 cells/ml were infected with baculovirus and grown at 27 °C for 60-70 hour before harvesting ...
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bioRxiv - Microbiology 2020Quote: ... high glucose (Gibco, 11965092) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... high glucose (Life Technologies) at 37°C and 5% CO2 ...
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bioRxiv - Plant Biology 2022Quote: ... high ROX (Thermo Scientific) on 2.5 μL of cDNA (diluted 1:20 ...
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bioRxiv - Cell Biology 2022Quote: ... high-BSA DMEM (Gibco) containing 10% FBS (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... high glucose (DMEM) (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2022Quote: ... high-glucose (Gibco 61965059), supplemented with 2% HI-FBS and 1% penicillin-streptomycin (Gibco 15140-122) ...
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bioRxiv - Microbiology 2023Quote: ... and high glucose (Gibco). WSN pcDNA3-based protein expression plasmids and pPol-based viral template RNA expression plasmids were described previously [61–63] ...
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bioRxiv - Neuroscience 2024Quote: ... DMEM/high glucose (Gibco) with FBS (10%)(Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... high glucose (Gibco, 11965092), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... α-SC35 (described previously98, Mouse 1:1000), α-NIN (Institut curie, Human 1:200), anti-Arl13b (proteintech, Rabbit, 1:4500) α-Rabbit Alexa594 (ThermoFisher, 1:400), α-Mouse IgG1 Alexa488 (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: Primary antibodies used in western blotting (WB) and immunofluorescence (IF) were anti-6x His mouse monoclonal (Invitrogen, #MA1-21315, 1:5000 in WB), anti-GST mouse monoclonal (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Immunology 2021Quote: All recombinant proteins were produced using Expi293F cells (Life Technologies). Spike proteins for enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Microbiology 2019Quote: ... and recombinant epidermal growth factor (10 ng/ml; Gibco-Invitrogen) at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... and recombinant epidermal growth factor (10 ng/ml; Gibco-Invitrogen) at 37°C with 5% CO2 ...
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: ... with RNaseOUT™ Recombinant Ribonuclease Inhibitor (Invitrogen cat# 10777-019).
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bioRxiv - Cell Biology 2021Quote: ... RNaseOUT recombinant ribonuclease inhibitor (200 U/mL; Thermo Fisher Scientific) and IGEPAL® CA-630 (0.5 % ...
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bioRxiv - Immunology 2021Quote: ... and recombinant epidermal growth factor (EGF, 2 ng/ml, Gibco).
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bioRxiv - Biochemistry 2020Quote: ... 40 U RNase inhibitor (RNaseOUT ™ Recombinant Ribonuclease Inhibitor, Invitrogen), and various amount of DNA sample (see figures) ...