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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... SK-N-AS cells were cultured in Iscove′s Modified Dulbecco′s medium (IMDM; Thermofisher) with 20% FBS (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... Enriched N-terminal peptides were then desalted with a C18 spin column (Thermo Fisher Scientific) and the eluate fraction was freeze-dried ...
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bioRxiv - Biophysics 2023Quote: ... The slides were then incubated for 5 min in 0.1 N HCl (Fisher Scientific; S25354) and washed with 2xSSC (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfection into cultured SK-N-AS cells was performed using Lipofectamine 2000 (Thermo Fisher Scientific) following standard protocols ...
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bioRxiv - Microbiology 2024Quote: ... N sgRNA was detected using TaqMan™ Universal PCR Master Mix (Applied Biosystems – cat # 4304437) and each reaction contain ...
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bioRxiv - Physiology 2020Quote: ... interleukin 6 (IL-6; Invitrogen, Carlsbad, CA), plasminogen activator inhibitor 1 (PAI-1 ...
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bioRxiv - Neuroscience 2024Quote: ... and granulocyte-macrophage colony-stimulating factor (GM-CSF, 10 ng/ml) to DMEM/F12 (HyClone™, SH30023.01) containing N-2 supplement (Gibco™, 17502048). This protocol facilitated the maturation process of the PMPs into microglia.
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bioRxiv - Neuroscience 2023Quote: ... (6) Negative photoresist SU-8 2000.5 was mixed with Lissamine rhodamine B ethylenediamine (RhBen; ∼10 μg/ml; Thermo Fisher Scientific) and placed in the dark at room temperature (RT ...
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bioRxiv - Biophysics 2022Quote: ... 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Catalog No. 22980) and Sulfo-NHS (N-hydroxysulfosuccinimide, Catalog No. 24510) were purchased from Thermo Scientific (Waltham, MA, US). Phosphate-Buffered Saline (PBS ...
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bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies, Darmstadt, Germany). Fold expression changes were determined using a standard 2−ΔΔCT method that compares CT (cycle threshold ...
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bioRxiv - Neuroscience 2019Quote: ... cycling synaptic vesicles were stained with the styryl dye N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino) styryl) pyridium dibromide (FM1-43; Molecular Probes, Inc., Eugene, OR). For labeling of synaptic vesicles ...
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bioRxiv - Bioengineering 2022Quote: ... A nanoViper Trap Column (C18, 3 μm particle size, 100 Å pore size, 20 mm × 75 μm ID; Thermo Fisher Scientific, P/N 164946) was used for desalting ...
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bioRxiv - Developmental Biology 2019Quote: ... The silicon device surfaces were functionalized with a previously described protocol using 3-mercaptopropyltrimethoxysilane (MPTMS) and N-gamma-maleimidobutyryloxysuccinimide ester (GMBS) NeutrAvidin-biotin chemistry (Thermo Fisher Scientific, Rockford, IL)[32 ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-N-WASP (Cell Signalling #4848S; 1:100) followed by Alexa Fluor 488 conjugated secondary antibodies (Invitrogen; 1:1000 in PBS). Actin tails were labeled with Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2022Quote: ... iAstrocyte differentiation was initiated by switching the medium to astrocyte medium (DMEM/F12+GlutaMAX, 1% N-2 supplement, 1% fetal bovine serum, all from Thermo Fisher Scientific) as previously described [81] and continued for at least 60 days ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI was used (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10 000, Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... Larval zebrafish at 6-7 dpf were paralyzed by immersion in 1 mg/ml alpha-bungarotoxin solution (Invitrogen) dissolved in external solution (in mM ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were counterstained using 4′,6-diamidin-2-phenylindol (DAPI, 1:1000, 7 min; D1306, Thermo Fisher Scientific). Washing steps were performed with PBS ...
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bioRxiv - Physiology 2022Quote: ... at 1-1.5 x 106 cells per well in William’s medium (catalog n°12551032, Gibco, Thermofisher Scientific, Waltham, MA, USA) supplemented with 5% FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... were used for studies of INS-1 cells treated with CSE or AC extract with or without 5 mM N-Acetyl Cysteine (NAC) from Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... M2DCTs with an MyBP-C antibody (N-terminal rabbit polyclonal, 1:1000, kind gift from Samantha Harris) and anti-rabbit AlexaFluor secondary (ThermoFisher). Intercalated disk images were obtained by labeling fixed M2DCTs with an N-cadherin antibody (1:200 ...
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bioRxiv - Microbiology 2022Quote: ... The blocking buffer was then aspirated and 50 μL of a 1:4000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid (N) antibody (MA536086, Invitrogen) in blocking buffer was added and incubated for 2 h at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... NPCs were plated on Geltrex in a 6 well plate in StemPro NSC SFM supplemented with 20ng/ml of EGF and bFGF for 2 days before switching to astrocyte differentiation medium consisting of DMEM with 1% N-2 supplement (Gibco), 1% GlutaMAX-I supplement (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... norvegicus with a HA-tag added to the C-terminal end representing the N-terminal domain (Met 1 to Val 469) of sAC (57) was cloned into pcDNA5/FRT (Invitrogen).
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bioRxiv - Neuroscience 2022Quote: ... Maturation medium I was DMEM/F12 supplemented with 100 U/ml Penicillin-Streptomycin and 1% N-2 Supplement (Gibco, 17502048). On day 10 ...
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bioRxiv - Cell Biology 2024Quote: ... at 4°C o/n and then with 40 μL of pre-blocked (1 mg/ml BSA) Dynabeads protein A (ThermoFisher). Beads were sequentially washed with Wash buffer 1 (20 mM Tris HCl pH 8.1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked for 2hrs at RT in 5% milk powder and then incubated overnight (o/n) at 4°C with primary rabbit anti-GFP antibody (1:1,000; Molecular Probes). Membranes were washed 3x for 10min in 1X TBST ...
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bioRxiv - Biochemistry 2024Quote: ... Labeling of WT and p.R196H actin at cysteine–374 using N–(1–pyrene)iodoacetamide (Thermo Fisher Scientific, Waltham, MA, USA) followed established protocols (55).
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bioRxiv - Neuroscience 2023Quote: ... and the QOLG-1 wild-type parent line (n=1) were karyotyped using a commercial service that provides information on chromosomal changes >1Mb resolution (KaryoStat Assay, ThermoFisher). Briefly ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Secondary antibodies were: goat anti-rat Alexa Fluor 647 (1:500, for anti-N-Cadherin, Life Technologies, cat. no. A21247), goat anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: Full-length cDNA sequence of Hs WDR23 Isoforms 1 and 2 were cloned into pcDNA 6.2/N-EmGFP/TOPO (Thermo Fisher), as previously described [19] ...
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bioRxiv - Immunology 2022Quote: ... sheared gDNA of each sample was split in two duplicates and samples were incubated o/n at 65°C after mixing with an equal volume hybridization buffer (1 ml composition: 667 µl 20x SSPE (Gibco); 267 µl 50x Denhardt’s solution (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with 4’,6-diaminodino-2-phenylindole (DAPI, Life Technologies D-21490, 1:2000) for 15 min ...
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bioRxiv - Physiology 2022Quote: ... 1mM isopropyl-b-D-thiogalactoside (IPTG, Fisher Scientific BP1755-10), 1.5% DMSO ...
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bioRxiv - Molecular Biology 2019Quote: ... either 50 µl (Figure 2A-2C, 7 and 8) or 25 µl (Figure 2D, 2E, 3-7 and 9) magnetic beads (Dynabeads protein G, Life Technologies #10004D) was used ...
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bioRxiv - Microbiology 2021Quote: ... Ten-fold serial dilutions (1×10−1 to 1×10−6) of virus stocks were prepared in MEM (supplemented with 25 mM HEPES (Gibco), 2mM L-Glutamine (Gibco) ...
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bioRxiv - Biochemistry 2019Quote: ... 7-hydroxy-4-cyanocoumarin (CHC) and BODIPY 577/618 maleimide were from Invitrogen/Molecular Probes Inc ...
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bioRxiv - Immunology 2022Quote: ... Design and Analysis QuantStudio 6/7 Pro Systems Software (Thermo Fisher Scientific, Version 2.5.0) was used to identify amplification of genes and calculate fold change from Cq values ...
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bioRxiv - Biochemistry 2023Quote: The RBD protein was incubated for 30 min at 21 °C and 750 rpm with a 10 molar equivalent of sulfo-m-maleimidobenzoyl-N-hydoxysuccinimide ester (Sulfo-MBS) (Thermo Scientific; MW 416.30). The excess crosslinker was removed by passing the protein through a PD-10 column using a PBS ...
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bioRxiv - Plant Biology 2023Quote: ... Equal amount of protein samples were electrophoresed on 10 % NuPAGE® Bis-Tris Gels in 1X MES [2-(N-morpholino) ethane sulfonic acid] SDS buffer (Invitrogen, Waltham, MA) and transferred to a nitrocellulose membrane (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... shTMEFF2-6 and shTMEFF2-9 sequences were generated using the siRNA Wizard online tool by Invitrogen: https://www.invivogen.com/sirnawizard/) ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used for immunoprecipitation and Western blotting: mouse N-cadherin (Thermo Fisher Scientific), HRP conjugated Strepavidin (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... adapter-ligated template was digested with 5 U of AmpErase Uracil N-Glycosylase (Life Technologies, N8080096). Libraries were then PCR amplified and indexed using Phusion Hot Start II High Fidelity Polymerase (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... 4 mice pooled per n) were deeply anesthetized and perfused intracardially with ice-cold dPBS (Gibco). Forebrain tissue were aseptically dissected ...
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bioRxiv - Developmental Biology 2021Quote: ... Flag-Tdrd3-N and Flag-Tdrd3-C were synthesized with an mMESSAGE mMACHINE SP6 kit (Ambion), and the resulting mRNAs (2 μg ...
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bioRxiv - Genomics 2020Quote: ... and total RNA was extracted from five (n=5) COVID-19 positive patients using TRIzol (Invitrogen) reagent following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and Kcns3neo/neo mice (n=5 per genotype) and homogenized in TRIzol reagent (Invitrogen, Carlsbad, CA). Total RNA samples were prepared from the homogenates using RNeasy lipid tissue mini kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... N-terminally HA-tagged AMPAR constructs were expressed from the doxycycline inducible pcDNA4/TO vector (Invitrogen Cat ...
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bioRxiv - Biophysics 2022Quote: DDM-solubilized claudin-4 was biotinylated using N-hydroxysuccinimide polyethylene glycol biotin (NHS-PEG4-biotin, ThermoFisher) by mixing 5.6 μM claudin-4 with 16.8 μM NHS-PEG4-biotin ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue or cells were lysed in N-PER™ Neuronal Protein Extraction Reagent (#87792, Thermo Fisher) supplemented with protease and phosphatase inhibitor cocktail (#78440 ...