Labshake search
Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Cell monolayers were then stained with 3 mL of overlay containing a 1:1 mixture of 1.2% oxoid agar with 4% neutral red (Gibco) and 2X DMEM with 2% (vol/vol ...
-
bioRxiv - Microbiology 2019Quote: ... day 1 (24 hours) and day 3 (72 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... a mix of PEImax (1 μg/μL) with expression plasmids (312.5 μg/L) in a 3:1 ratio in OptiMEM (Gibco) were added to the cells ...
-
bioRxiv - Immunology 2022Quote: Supernatants were collected 3 days post Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco) stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel ...
-
bioRxiv - Immunology 2022Quote: ... in the presence of T cells at a 1:1 effector:target cell ratio in the presence of the caspase-3/7 Green Detection Reagent (Invitrogen). Images were taken every 2 h at 10× magnification ...
-
bioRxiv - Microbiology 2023Quote: ... containing 3 mL of home-made Expansion Medium consisting of a 1:1 mixture of DMEM/F-12 (Gibco) and Neurobasal Medium (Gibco ...
-
bioRxiv - Neuroscience 2024Quote: ... 10 mM NaCl, 3 mM MgCl2, 1 mM dithiothreitol, 1% BSA, 0.1% Tween 20, and 0.6 U/mL RiboLock [Thermo Fisher]). Next ...
-
bioRxiv - Pathology 2024Quote: ... Cells were subcultured at a 1:3 to 1:6 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
-
bioRxiv - Neuroscience 2024Quote: ... The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
-
bioRxiv - Biophysics 2019Quote: ... and CY-3 conjugated goat anti-mouse (Thermo Fisher Scientific, Waltham, USA, 1:300), for actin ...
-
bioRxiv - Biochemistry 2020Quote: ... Cells were split every 3 days at 1:10 using Trypsin-EDTA (ThermoFisher Scientific). On day 1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... 1/10th volumes of 3 M Sodium Acetate and 30 μg Glycoblue coprecipitant (Ambion).
-
bioRxiv - Developmental Biology 2022Quote: Testes were dissected from 1-3 day old males in SF-900 media (Gibco) then fixed in 4% formaldehyde diluted in PBS for 20-30 minutes and permeabilized in 1% PBT (triton x100 diluted in PBS ...
-
bioRxiv - Plant Biology 2019Quote: ... in hydrochloric acid 0.01 N (1:3, v:v) (Fisher Scientific; Hampton, New Hampshire, USA).
-
bioRxiv - Bioengineering 2020Quote: ... The epithelium medium was composed of a 3:1 mixture of DMEM (Hyclone, ThermoFisher) combined with Ham’s F12 medium (Gibco ...
-
bioRxiv - Neuroscience 2021Quote: ... Axotomy was performed using 3 different methods: 1) Trypsin-EDTA 0.25% (Thermo Fisher Scientific) treatment for 15 min (Figure 4C ...
-
bioRxiv - Cell Biology 2021Quote: ... 1-3 µg were reverse transcribed with a MuLV reverse transcriptase (Applied Biosystems, UK) using random primers (Bioline ...
-
bioRxiv - Molecular Biology 2022Quote: ... using NuPAGE 3-8% Tris-Acetate 1 mm 10-well gels (Thermo Fisher Scientific) run at 150 V for 90 minutes in 1× NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific) ...
-
bioRxiv - Systems Biology 2020Quote: ... Organoids were washed and fresh medium containing TOPRO-3 Iodide (1/1000, Molecular Probes) was added ...
-
bioRxiv - Cancer Biology 2020Quote: ... sonicated (3 × 0.5s pulses) with a probe sonicator (Level 1; Fisher Scientific, Waltham, MA) and quantified using a Pierce™ 660 nm assay (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2020Quote: ... the chambers were washed 3 times using 1× Hank’s balanced Salt Solution (HBSS, Gibco) and blocked with 1% Bovine Serum Albumin (BSA ...
-
bioRxiv - Developmental Biology 2021Quote: ... this included TO-PRO®-3 Iodide nuclear dye (T3605, Life Technologies 1:10,000). Slides were washed 3x PBST prior to mounting with anti-fading PVA/Moviol-DABCO (Sigma ...
-
bioRxiv - Molecular Biology 2021Quote: ... then incubated in 1 μM TO-PRO-3 (T3605; Thermo Fisher Scientific, Waltham, MA) in PBS for 15 minutes ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were transfected with 1 µg total DNA and 3 µL Lipofectamine 2000 (Invitrogen). Media was changed 4 h post-transfection to standard maintenance media and cells were harvested for microscopy or western blot 24 h post-transfection.
-
Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... cerebella were washed 3 × 1 h in 50% OCT (Fisher Scientific, 23-730-571) before embedding in 100% OCT ...
-
bioRxiv - Microbiology 2022Quote: ... rabbit anti-claudin 3 polyclonal antibody (1:100 dilution, PA5-16867, Thermo Fisher Scientific); mouse anti-chondroitin sulfate antibody (1:100 dilution ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Slices were finally incubated for 3-6 minutes with DAPI (1:10 000, Invitrogen) diluted in PBS and mounted on Polysine® Slides stored at 4°C until imaging.
-
bioRxiv - Neuroscience 2023Quote: ... Brain sections were incubated with rabbit anti-Neuroligin-3 (1:250, Thermo Fisher Scientific) overnight at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... Primed mESCs were passaged every 3 days using 1 U/ml of Dispase (Gibco) as a colony detachment reagent ...
-
bioRxiv - Cell Biology 2024Quote: ... for 3 min and 1:500 of Hoechst 33342 (20 mM, Thermo Fisher Scientific) for 10 min ...
-
bioRxiv - Physiology 2023Quote: ... and Cy-3 conjugated anti rabbit IgG at (dilution 1:300) (A10520, Thermo Fisher) for 1 hour at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... Stable polyclonal populations of cells were selected using 3 μg ml-1 puromycin (Invitrogen).
-
bioRxiv - Bioengineering 2023Quote: ... Invitrogen) was diluted 1:250 in 3% BSA and 0.1% Tween 20 (Thermo Scientific) in PBS (IF solution) ...
-
bioRxiv - Cell Biology 2024Quote: 3×106 TRExRTA BCBL-1 cells in 100 μL of resuspension buffer T (Invitrogen) were mixed with 3 μL of 100 μM siRNA stock of the following Dharmacon siRNAs ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL NT-3 (PreproTech) and 1 µg/mL Laminin (Gibco, Thermo Fisher) plus DOX ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL NT-3 (PreproTech) and 1 µg/mL Laminin (Gibco, Thermo Fisher) plus DOX ...
-
bioRxiv - Immunology 2024Quote: ... using PEI (3:1 ratio) for transfection of 293F cells and ExpiFectamine (ThermoFisher, #A14525) for transfection of Expi-293F cells according to the manufacturer’s suggestion ...
-
bioRxiv - Microbiology 2021Quote: ... FluoZin-3 (FluoZin™-3, AM, cell permeant, Thermo Fisher) was added at 1 mM ...
-
bioRxiv - Biochemistry 2023Quote: ... DiIC12(3) (1,1’-Didodecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate) (Invitrogen, D383) was used ...
-
bioRxiv - Developmental Biology 2023Quote: ... mounting 3 dpf embryos in 3% methylcellulose (Thermo Scientific, 258111000). After imaging ...
-
bioRxiv - Microbiology 2019Quote: ... with different spectral signatures selected for the detection of PfHRP2 and PAN-pLDH were washed with distilled water and activated with Sulfo-NHS (N-hydroxysulfosuccinimide) and EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride) (Pierce, Thermo Fisher Scientific Inc., Rockford, IL), both at 50 mg/mL ...
-
bioRxiv - Neuroscience 2021Quote: ... in Tris-buffered PBS/ 0.1 % Tween 20 for 1 h at room temperature they were incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; 49 kDa; 1:1000; Thermo Fisher Scientific, Waltham, MA, USA), mouse monoclonal anti-myelin-associated glycoprotein (MAG ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5’ CAGACAGUGCAGUAUUCACTT 3’) and control siRNA (#4427037) as in (56) and OSR1 siRNA (s19303 Silencer® Select) were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... sonicated 3 three times for 5 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 μl (1 μg/μl ...
-
bioRxiv - Immunology 2021Quote: ... cells were washed and fed with the complete Lifeline medium plus antibiotics and transfected with 50 nM custom mirVana miRNA inhibitor (inhibitor sequence: 5′-GAGCCCGACGAGCUACCAGACUGCUCCA-3′, #4464086, Thermo Fisher Scientific) or 50 nM mirVanna inhibitor negative control#1 (#4464077 ...
-
bioRxiv - Genetics 2021Quote: ... the extracted DNA was directly Sanger sequenced from in 5’ and 3’ directions for each sample with the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol with the same primers as for the amplification (Prdm9ZnFA_Bal_R/Prdm9ZnFA_Bal_F) ...
-
bioRxiv - Microbiology 2021Quote: ... Ten nanograms of the extracted RNA samples were reverse-transcribed using a Uni-12 primer (5′-AGCRAAAGCAGG-3′) and Superscript III reverse transcriptase (Thermo Fisher Scientific). Ten-fold diluted cDNAs were PCR amplified using KOD FX (Toyobo ...
-
bioRxiv - Cell Biology 2021Quote: ... or siGENOME SMARTpool siRNA D-019800-17 DSP (Dharmacon, Lafayette, CO) (siDP1) or stealth siRNA oligonucleotides 5′-CAGGGCUCUGUCUUCUGCCUCUGAA-3′ from Life Technologies (siDP2) using the Amaxa Nucleofector System (Lonza ...
-
bioRxiv - Microbiology 2019Quote: ... and antisense primer 3Vif (5-AGCTAGTGTCCATTCATTG-3) using a Superscript III single RT-PCR system with Platinum Taq DNA polymerase kit (Thermo Fisher Scientific) as per the manufacturers instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... were co-electroporated with the gRNA-5’/3’-expressing construct and a circular donor plasmid carrying the synthesized knock-in template (GeneArt®Invitrogen™).