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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Cell lysates were pre-cleared using protein A beads (Invitrogen). Pre-cleared lysates were immunoprecipitated using anti-lamin B1 antibody and these immunoprecipitated samples were incubated with protein A-sepharose beads (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: All proteins were expressed in BL21 DE3 star cells (Invitrogen), grown at 37°C (in LB media for unlabeled proteins and in M9 media for C-13 or N-15 labeled proteins ...
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bioRxiv - Immunology 2019Quote: ... Proteins were expressed in FreeStyle 293F cells (Thermo Fisher - R79007). About 25 mL Opti-MEM (Life Technologies – 31985-070 ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were placed in PFHMII protein free medium (GIBCO, ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were expressed in Expi293 suspension adapted cells (ThermoFisher Scientific). These cells were cultured in Expi293™ Expression Medium and transfection was achieved by mixing 2µg of cDNA with 6µg of polyethylenimine (PEI ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant protein was expressed using Expi293F™ Cells (ThermoFisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... RBD proteins were purified from transiently-transfected Expi293F cell (Gibco) supernatants by nickel affinity and size-exclusion chromatography (47) ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were placed in PFHMII protein free medium (GIBCO, ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the protein was overexpressed in BL21(DE3)E.coli cells (Invitrogen) in Lysogeny Broth (LB ...
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bioRxiv - Microbiology 2021Quote: The proteins were expressed in Expi293 cells (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Protein expression was carried out using FreeStyle 293F cells (ThermoFisher) transiently co-transfected at a 4:1 ratio of hMPV F and furin-expressing plasmids using polyethylenimine (PEI) ...
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bioRxiv - Biochemistry 2021Quote: ... PARP1 and PARP2 proteins were produced in Sf9 cells (ThermoFisher) using a baculovirus expression system ...
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bioRxiv - Biochemistry 2021Quote: ... Cell Light-Red Fluorescent Protein (RFP) reagents (Thermo Fisher Scientific) were used for staining the early endosome ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transfection with purified plasmid using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were placed in PFHMII protein free medium (GIBCO, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: Total proteins were extracted from cells using RIPA buffer (Invitrogen) supplemented with a protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2022Quote: Protein constructs were expressed in Spodoptera frugiperda (Sf9) cells (Gibco) using the baculovirus system as previously described (Toropova et al. ...
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bioRxiv - Physiology 2022Quote: Recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transient transfection ...
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bioRxiv - Immunology 2024Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher, Cat. #A14635) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were expressed by transient transfections in Expi293F cells (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and all mammalian protein expression with Expi293F cells (ThermoFisher Scientific). All bacterial expression was performed from a pET29b(+ ...
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bioRxiv - Cancer Biology 2023Quote: The Subcellular Protein Fractionation Kit for Cultured Cells (#78840, Thermofisher) was used following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... FAK protein expression was done by Expi293F cells (Invitrogen; A14527). The Expi293F cells were grown in Union-293 media (Union-Biotech ...
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bioRxiv - Biochemistry 2023Quote: All proteins were expressed in BL21 DE3 star cells (Invitrogen), grown at 37°C (in LB media for unlabelled proteins and M9 media for 13C or 15N isotope-labeled proteins ...
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bioRxiv - Biophysics 2023Quote: All G proteins were expressed recombinantly in TOP10 cells (ThermoFisher). 0.5 L cultures were auto-induced for 72 h at room temperature in 2.4 L Erlenmeyer flasks with media containing 2% tryptone ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were extracted from cells using RIPA buffer (Thermo Fisher). Bolt Bis-Tris Plus Mini Protein Gels ...
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bioRxiv - Cancer Biology 2023Quote: Cell proteins were extracted using RIPA Lysis Buffer (Thermo Fisher) with freshly added protease/phosphatase cocktail inhibitor 100x (Cell Signalling Technology) ...
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bioRxiv - Cancer Biology 2023Quote: Cell proteins were extracted using RIPA Lysis Buffer (Thermo Fisher) with freshly added protease/phosphatase cocktail inhibitor 100x (Cell Signalling Technology) ...
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bioRxiv - Physiology 2024Quote: Total cell protein was extracted in RIPA lysis buffer (ThermoFisher) and aliquots of each sample were digested with trypsin (2.5µg per 100µg protein ...
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bioRxiv - Genetics 2024Quote: Pierce Cell Surface Protein Biotinylation and Isolation Kit (Thermo Scientific) was used to biotinylate and isolate cell surface proteins ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary neurospheres grown for 3 days (3-4 million cells) were harvested and crosslinked with 0.5% formaldehyde (Thermo Fisher, cat. no. 28908) in 0.1 M DPBS for 5 min at RT with soft agitation ...
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bioRxiv - Cancer Biology 2019Quote: ... (5’-CUAUGAACAUAAAGUCUGCTT-3’) or its non-specific control (5’-UUCUCCGAACGUGUCACGUTT-3’) were transfected into cells using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA).
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bioRxiv - Microbiology 2021Quote: Vero81 cells were adsorbed with CHIKV strains diluted in virus dilution buffer (VDB, RPMI medium with 25 mM HEPES [Gibco] supplemented to contain 1% FBS) at a multiplicity of infection of 0.01 plaque-forming units (PFU)/cell at 37°C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Pyrene-labeled virus was produced in BHK-21 cells cultured beforehand in the presence of 15 μg/ml 1-pyrenehexadecanoic acid (Thermo Fisher Scientific, Waltham, MA, USA). The supernatant was harvested at 48 hpi ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 50 µg of proviral plasmid was transfected into 107 HEK293T/17 cells for virus production using Lipofectamine 2000 (Thermo Fisher Scientific, Cat# 11668-027). The next day ...
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bioRxiv - Immunology 2021Quote: ... qPCR was performed using TaqMan Fast virus 1 Step PCR Master Mix (Life Technologies), standard curve was drawn with 2019_nCOV_N Positive control (IDT) ...
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bioRxiv - Immunology 2022Quote: ... amplified using the TaqMan Fast Virus 1-Step Master Mix qRT-PCR kit (Invitrogen) on a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... probes and TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems, Foster City, CA). We used pre-combined probe and primers (500 nM primers and 250 nM probe ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Ambion-ThermoFisher Scientific) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Ambion-ThermoFisher Scientific) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... while 4x Taqman Fast Virus 1-Step Master Mix (Life Technologies, Carlsbad, CA, USA) was used for RNA enteric viruses ...
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bioRxiv - Immunology 2021Quote: ... then incubated with the appropriate amount of virus diluted in Fluorobrite DMEM (Life Technologies) medium supplemented with 2% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNA was quantified using qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2021Quote: For Sendai virus iPSC reprogramming using CytoTune™-iPS 2.0 Sendai Reprogramming kit (Invitrogen), fibroblasts were reprogrammed as previously described (Milagre et al. ...
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bioRxiv - Immunology 2022Quote: ... RNA from virus stocks was purified using TRIzol Reagent (ThermoFisher Scientific, catalog no. 15596026). Brief ...
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bioRxiv - Immunology 2022Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and gene-specific probes and reverse primers as follows:
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bioRxiv - Microbiology 2022Quote: ... using TaqMan Fast Virus 1-Step Master Mix (Cat. n° 4444434, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs were synthesized using random primers and Moloney murine leukemia virus RT (Invitrogen). The RT-PCR products were separated on a 2% w/v agarose-TBE gel ...
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bioRxiv - Neuroscience 2020Quote: ... Fibroblasts were reprogrammed into iPSCs by using the replication-incompetent Sendai virus kit (Invitrogen) according to manufacturer’s instructions ...