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Citations for Thermo Fisher :
1101 - 1150 of 6146 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Cortical hemispheres were dissected and homogenized in ice-cold homogenization buffer (N-PER Neuronal Protein Extraction Reagent (ThermoFisher Scientific) with cOmplete Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Developmental Biology 2019Quote: ... GenBank accession number BC057894.1) with an N-terminal hemagglutinin (HA) tag were reverse transcribed with SuperScript III Reverse Transcriptase (Invitrogen) and PCR-amplified using Phusion high-fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: Each sample (n=5) was placed in a 2 mL centrifuge tube containing 320 μL of collagenase II (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2021Quote: N-terminal GST-tagged MBNL1 (amino acids 1-260)59 was recombinantly expressed using BL21 Star (DE3) cells (Invitrogen) and protein expression induced using 0.5 mM IPTG at an OD600 = 0.6-0.7 for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... NosRcaΔC and NosRca-IFmut was labelled at the N terminus with the fluorophore Alexa Fluor 405 NHS ester (ThermoFisher) (∼2.2 ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor® 555 goat anti rabbit IgG (Cat. N° A21429) was obtained from Life Technologies (Carlsbad, CA, USA), Alexa Fluor® 488 Goat Anti-Mouse IgG (Cat ...
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bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... over the course of 28 days and healthy controls (n = 37) and hybridized onto an HU133 Plus 2.0 GeneChip (Affymetrix) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2021Quote: ... Tyr-nitration of MSD2 was detected after SDS-PAGE with anti-Nitrotyrosine primary antibody (Invitrogen, cat. n. A-21285) diluted to 1:3,000 in TBST with 3% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... and the N-terminus of the construct was attached to a biotinylated DNA-coated paramagnetic bead (2.8 μm diameter, Invitrogen) via a biotin-neutravidin interaction ...
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bioRxiv - Cell Biology 2021Quote: ... and 2-NDBG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose] (N13195) were purchased from Invitrogen. Recombinant murine SCF (250-03) ...
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bioRxiv - Biophysics 2020Quote: A1-LCD was fluorescently labeled on the N-terminus using Alexa Fluor® 488 NHS ester fluorescent dye (ThermoFisher) to label the primary amines of proteins ...
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bioRxiv - Microbiology 2022Quote: ... The pellet containing the membranes was resuspended in 20 mM phosphate buffer pH 7.4 with 0,3% n-octyl-poly-oxyethylene (OPOE) detergent (Affymetrix, UK) and incubated at 20°C for 2 h under agitation to separate inner (IM ...
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bioRxiv - Cell Biology 2022Quote: HEK 293T cells were transfected with 1 µg of plasmids containing either FL (wild-type), various deletions (ΔTM, ΔNΔTM, NCC, CC, N) or mutant FLAG-KASH5 constructs with Lipofectamine LTX (Invitrogen) transfection reagent using the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary antibody O/N at 4°C (5% FBS, 0.5% Tx, 0.2% gelatine in PBS; GFP (1:500, ThermoFisher Scientific). Slices were then repeatedly washed with PBS-0.5% Tx ...
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bioRxiv - Biophysics 2022Quote: The human wild-type S1PR5 gene (UniProt ID Q9H228) with an N-terminal 3 × HA epitope (YPYDVPDYA) tag was cloned into pcDNA3.1+ (Invitrogen) at KpnI(5’ ...
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bioRxiv - Microbiology 2021Quote: ... Taqman gene expression assays for N2 and N3 regions of the SARS-CoV-2 N gene were made (ThermoFisher) following CDC specifications (Division of Viral Diseases ...
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bioRxiv - Immunology 2021Quote: ... of M13KO7 helper phage and grown o/n at 37°C in fresh 2xYT medium supplemented with Amp100 and 75μg/mL of kanamycin (Kan75, Gibco) to select for bacteria containing a phagemid and infected with a helper phage ...
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bioRxiv - Molecular Biology 2020Quote: ... The peptides were separated on an in-house made C18 column (20 cm length, 75 μm inner diameter, 1.9 μm particle size) by an easy n-LC 1200 (ThermoFisher) and directly injected in a QExactive-HF or in case of TMT samples into a Fusion Lumos mass-spectrometer (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... N gene reverse primer (5’-GAGGAACGAGAAGAGGCTTG-3’) and probe (5’-FAM-ACTTCCTCAAGGAACAACATTGCCA-QSY-3’) using Taqman mastermix (Thermo Fisher). The thermal cycling steps were ...
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bioRxiv - Microbiology 2022Quote: ... offline high pH reverse-phase fractionation was performed using a Hypersil Gold column (Thermo Scientific, P/N 25002-202130). The mobile phase A was water adjusted with ammonium hydroxide to pH 10 and B was 10 mM ammonium bicarbonate in 80% Acetonitrile (ACN ...
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bioRxiv - Genomics 2022Quote: ... An N-terminal FLAG tag and a nuclear localization signal (NLS) along with the M.CvIPI ORF were then cloned N-terminal to Lamin B1 into pENTR (Invitrogen) using Gibson cloning (New England Biolabs) ...
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bioRxiv - Systems Biology 2022Quote: ... streptavadin magnetic beads were aliqoted and washed in 0.1 N NaOH followed by two washes in 1× SSC buffer (Invitrogen). Hybridized RNA and probes were diluted to the original bead volume using a 1 × SSC and 20% formamide solution and incubated at room temperature for 3 minutes prior to bead capture ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million SK-N-BE(2)-FLUC cells were stained immediately before injection with CellTrace™ Far Red (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The gel was then transferred to Hybond N+ (Amersham) nylon membrane and blotted with HRP-Conjugated Streptavidin (Thermo Scientific) with a dilution factor 1:2000 ...
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bioRxiv - Bioengineering 2020Quote: ... Proliferation was assessed using a Click-iT EdU Alexa Fluor 594 Imaging Kit (ThermoFisher; n=3/group/time point). EdU was added to media at a concentration of 10 µM at days 2 and 6 of culture and incubated for 24 hours before gels were fixed at days 3 and 7 ...
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bioRxiv - Genomics 2019Quote: ... two similar arrays were used for targeted genotyping within the study population: the UK BiLEVE Axiom Array (n=49,950) by Affymetrix and the UK Biobank Axiom Array (n=438,427) ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were obtained from embryonic cortices (n=10-12/cell preparation) dissected in HBSS (Life Technologies, Carlsbad, CA, USA) from timed pregnant Sprague Dawley rats (Harlan Laboratories ...
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bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
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bioRxiv - Genetics 2021Quote: ... We dissected the facial primordia (n = 5 from each breed) and stored the tissue in RNAlater (Thermo Fisher Scientific) at −80°C ...
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bioRxiv - Biochemistry 2021Quote: ... the Lambda DNA in TE from Quant-iT ™ PicoGreen® dsDNA Assay Kit (ThermoFisher Scientific, cat. n. P11496) was used ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
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bioRxiv - Immunology 2020Quote: The validation cohort included a collection of selected G1 and G3 ccRCC tissues (n = 14 each) and corresponding normal kidney tissue (n = 14) microarray data (“Rostock cohort”, Array GeneChip HG U133 Plus 2.0, Affymetrix) (Maruschke et al. ...
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bioRxiv - Neuroscience 2019Quote: ... The antigen peptide with N-terminus Cysteine (NH2-CGGGGAAPGPPRHG-COOH) was coupled with keyhole limpet hemocyanin (Thermo fisher 77600). The antigen was injected into rabbit and antisera were collected after primary and several booster injections ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was isolated from ontogenetic and regenerating scales (n=3 fish per group, RU) by using Trizol (Invitrogen) for RNA-sequencing (RNA-seq ...
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bioRxiv - Physiology 2020Quote: ... muscles were stained for 3 minutes with 10μM 4-(4-diethylaminostyrl)-N-methylpyridinium iodide (4-Di-2ASP, Molecular Probes) to allow imaging muscle with an upright epifluorescence microscope (Leica DMR ...
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bioRxiv - Biochemistry 2021Quote: ... The construct with a N-terminal 8xHis-TwinStrep-GFP-tag were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in black 96 well plates with transparent bottoms ...
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bioRxiv - Biochemistry 2021Quote: ... H3.3 was cloned into pLEX_305-C-dTAG and p53 as well as RelA were cloned into pLEX_305-N-dTAG using Gateway LR clonase II Enzyme mix kit (Invitrogen). The sequences of the primers are listed below.
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bioRxiv - Cell Biology 2021Quote: ... or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine) (Invitrogen, D3521) together with 5 µM delipidated BSA (as a delivery carrier ...
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bioRxiv - Cell Biology 2021Quote: ... WT and SIRT1 KO mESCs cultured in serum-free ESGRO medium or serum-containing M10 medium were incubated with 5 µM BODIPY™ FL C5-Sphingomyelin (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosyl Phosphocholine) (Invitrogen, D3522) or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine ...
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Immunology 2021Quote: ... Molecular detection of SARS-CoV-2 was performed with TaqMan™ Gene Expression Assay (Cat. N. 4331182, ThermoFisher®) with specific primers/probes previously described40 ...
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bioRxiv - Genetics 2020Quote: Total RNA was isolated from gonadal adipose tissue (eWAT; n = 9) samples using the Ambion RiboPure (Thermo Fisher Scientific). RNA was quantified in a Nanodrop ND-1000 spectrophotometer and RNA purity and integrity was checked by using a Bioanalyzer-2100 equipment (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... we resuspended the frozen nuclei fractions in 300 μL of N-PER™ Neuronal Protein Extraction Reagent (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (16:0 NBD) were purchased from Invitrogen. Phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: Large-scale production of bio-inactive human N-SLIT2ΔD2 was performed by transfecting HEK293F cells with human N-SLIT2ΔD2 cDNA (1 ug/mL) using PEI reagent (Polyethyleneimine, linear, M.W. 25,000, Thermo Fisher Scientific) (19) ...
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bioRxiv - Genetics 2022Quote: Our gene interaction-perturbation pipeline applied the discovery cohort (n =2,167) composed of 19 independent datasets from the same chip platform (Affymetrix Human Genome U133 Plus 2.0 Array ...
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bioRxiv - Immunology 2022Quote: ... and excess N-ethylmaleimide was removed by filtration with Zeba spin columns with a 7kDa cut-off (Thermo Fisher). Sulfenic acid residues were selectively reduced with 200 mM sodium arsenite and newly released free thiols were labeled by adding 200 mM 1mM biotin–maleimide ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.