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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... mixed with PBS (4:1) adjusted to a concentration of 17,000 cells/µl and cultured in culture medium Advanced DMEM/F12 (Gibco), Primocin 50 µg/ml (Invivogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... the medium was changed to differentiation medium containing DMEM/M199 at a 4:1 ratio (Gibco, 10566016 and 31150022), 20 mM Hepes (Biochrom ...
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bioRxiv - Immunology 2023Quote: ... and packaging vector psPAX2 were mixed in a 4:3:1 ratio in OPTI-MEM (Thermo Fisher Scientific, 31985070) and PEI (Polysciences ...
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bioRxiv - Developmental Biology 2023Quote: ... Cell pellets were finally resuspended in wash buffer containing 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) or 7-AAD viability dye (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM MnCl2 with a final assay concentration of 75 µM 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) (Invitrogen D6567). Generation of 6,8-difluro-4-methylumbelliferone (DiFMU ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg or 4 µg of modRNA was mixed with Opti-MEM reduced serum medium plus GlutaMAX (ThermoFisher Scientific) and Lipofectamine RNAiMAX (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... MDA-MB-231 cells were co-transfected with pXPR_001-sgRab21 and pUC57-Rab21 HDR plasmids in 1:4 ratio using Lipofectamine 3000 (Invitrogen). Five days post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... cells were plated at 1×106 in 4 10cm plates 24 hours prior to harvest through trypsinization (ThermoFisher Scientific) for adherent cells ...
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bioRxiv - Cell Biology 2024Quote: ... 1:1000 primary antibody overnight at 4°C and a AlexaFluor goat anti-rabbit secondary antibody (ThermoFisher, A-11008) at 1:500 for 4 hours at RT with PBS washes in between antibody steps.
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bioRxiv - Developmental Biology 2024Quote: Tissue samples were fixed overnight at +4°C in periodate–lysine–paraformaldehyde (PLP) buffer [1% paraformaldehyde (Thermo Scientific, 28908), 0.01M sodium periodate (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... Protein (10 μg) from each sample was combined with 1X Bolt LDS Sample Buffer (1:4, Invitrogen catalog #B0007) and 1X Bolt Reducing Agent (1:10 ...
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bioRxiv - Bioengineering 2024Quote: ... PEGαMA hydrogels were made by dissolving the PEGαMA in pH 8.4 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Life Technologies) at 12.5-15.5 weight percent (wt%) ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred to a 0.2µm or 0.4μM PVDF membrane that was pre-soaked in MeOH for 1min and transferred at 30V or 200mA for 1-1.5hour at 4°C in NuPAGE Transfer Buffer (Invitrogen) containing 20% MeOH ...
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bioRxiv - Microbiology 2024Quote: ... that had been previously coated with 1 𝜇g/mL NeutrAvidin (Thermo Fisher, cat. A2666, coated overnight at 4°C) and blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then incubated overnight at 4°C with primary antibodies directed against RAB11 (Invitrogen 71-5300, 1:100), CLIMP63 (R&D systems AF7355 ...
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bioRxiv - Immunology 2024Quote: ... or stimulated with anti-CD3/CD28 Dynabeads at a 4:1 bead:cell ratio (Fisher Scientific, cat#: 11-452-D).
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bioRxiv - Microbiology 2024Quote: ... In brief washed RBCs from each donor was stained with 1 µM Fluo-4-AM dye (Molecular Probes/Invitrogen) for 30 min at 37°C in dark ...
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bioRxiv - Microbiology 2024Quote: ... In brief washed RBCs from each donor was stained with 1 µM Fluo-4-AM dye (Molecular Probes/Invitrogen) for 30 min at 37°C in dark ...
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bioRxiv - Microbiology 2024Quote: ... Microorganisms were visualised using the membrane stain FM 4-64 (10 µg/ml in 1 x PBS; T13320, Invitrogen). DNase I (04716728001 ...
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bioRxiv - Immunology 2024Quote: ... Membranes were stained o/n at 4°C with primary antibody rabbit chromogranin A (1:1000, Invitrogen; PA5-35071) or rabbit catestatin (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Tissues were stained overnight at 4°C with goat anti-mouse Ifn-λr1 polyclonal antibody (1:300 dilution, Invitrogen), rabbit anti-mouse uteroglobin polyclonal antibody (1:300 dilution ...
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bioRxiv - Immunology 2024Quote: ... that had been previously coated with 1 μg/mL NeutrAvidin (Thermo Fisher, cat. A2666, coated overnight at 4°C) and blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... slices were washed and stained for 1-4 hours with goat anti-chicken Alexa Fluor 488 (A-11039, Invitrogen) secondary solution ...
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bioRxiv - Physiology 2024Quote: ... Slides were then stained with DAPI (1:10,000; 4’ ,6-diamidino-2-phenylindole; cat. no. D3571; Thermo Fisher Scientific) for 10 minutes at room temperature and mounted with glass coverslips using 1:1 PBS and glycerol as mounting medium ...
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bioRxiv - Molecular Biology 2024Quote: ... A ∼4 mm piece of aorta was cut and stained with DAPI (1:5000 dilution in PBS, D3571, Invitrogen) for 5 minutes ...
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bioRxiv - Physiology 2024Quote: ... Slides were then stained with DAPI (1:10,000; 4’, 6-diamidino-2-phenylindole; Cat. No. D3571; Thermo Fisher Scientific) for 10 minutes at room temperature and mounted with glass coverslips using 1:1 PBS and glycerol as mounting medium ...
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bioRxiv - Biochemistry 2024Quote: ... Cell debris was removed by centrifugation 17.000 rpm at 4°C for 1 h (A27 rotor; Thermo Scientific™). The supernatant was loaded on a 5-ml His Trap HP Ni-NTA column (GE Healthcare ...
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bioRxiv - Bioengineering 2024Quote: ... samples were incubated overnight at 4°C with Alexa 647 donkey-α-rabbit (1:500 in blocking solution; Invitrogen) or Alexa 647 donkey-α-mouse (1:500 in blocking solution ...
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bioRxiv - Immunology 2024Quote: ... incubated overnight at 4°C followed by secondary antibodies (Goat anti-mouse-AlexaFluor 647, 1:250, ThermoFisher #A-21236) and Phalloidin-iFluor488 (1:1,000 ...
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bioRxiv - Genetics 2024Quote: ... 762Q84] was used to stop cross-linking and nuclei were centrifuged (4 minutes at 1300 x g at 4°C) and resuspended in 100 µL Wash Buffer (1 mL 1M HEPES pH 7.5 [ThermoFisher, J60712.AP] ...
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bioRxiv - Biophysics 2024Quote: ... in 1X PBS at 37 °C for 1 min followed by 4% methanol free paraformaldehyde (16% solution, Thermo Scientific) in 1X PBS at 37°C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... 500 μM triazole ligand Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amin (TBTA, Thermo Fisher Scientific, 454531000), 62.5 μM biotin-alkyne tag (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then mounted onto plain 25 × 75 × 1 mm microscope slides (Catalog No: 12-544-4; Fisher Scientific). Time-lapse images were acquired with an inverted Nikon Eclipse Ti2-E microscope with a Yokogawa confocal scanner unit (CSU-W1) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated overnight at 4°C in 1X PBS containing MAP2 monoclonal antibody (1:300; ThermoFisher, #13-1500). The next day ...
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bioRxiv - Cancer Biology 2024Quote: DNA extraction was performed on 4 flash frozen mouse medulloblastomas and matched forebrain normal controls using UltraPure phenol:chloroform:isoamyl alcohol (25:24:1) (ThermoFisher 15593031). Frozen tumor tissue was homogenized with bead-beating using the Qiagen TissueLyser II in Qiagen DNeasy Blood and Tissue Kit Buffer ATL with proteinase K added ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibody incubation was then done for 1 hour at 4°C with Alexa fluor 488/568/633 (Invitrogen) at 1:400 dilution of the desired host ...
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bioRxiv - Developmental Biology 2023Quote: ... the cells were incubated at 4□ for 30 min with a PE-conjugated anti-Tra-1-60 antibody (Thermo Fisher Scientific, 12-8863-82, 1:5) or a PE-conjugated anti-SUSD2 antibody (Biolegend ...
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bioRxiv - Molecular Biology 2023Quote: ... the tissues were incubated at 4°C for 1 day with the following secondary antibodies as appropriate: Alexa 546-conjugated anti-mouse IgG (Thermo Fisher, A11030, 1:250–1000 dilution), Alexa 488-conjugated anti-mouse IgG (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were blocked with 5% BSA at room temperature for 1 h and incubated with the following primary antibodies overnight at 4°C: mouse anti-v5 (Thermo Fisher Scientific, Cat. # R96025; 1:2000), mouse anti-Flag M2 (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with anti-CD31 (1:300) overnight at 4°C followed by Alexa Fluor-594 secondary antibodies (Thermo Fisher 1:500, 30 min at RT) as recommended (In Situ Cell Death Detection Kit ...
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bioRxiv - Bioengineering 2023Quote: Passage 3 and passage 4 cells from 8 donors (4 male and 4 female) were expanded in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) and were cultured until passage 5 (p3-5 and p4-5 ...
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bioRxiv - Cell Biology 2021Quote: ... PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-amino-carbamoyl)methoxy)-phenyl)oxazole] (ThermoFisher Scientific, USA) was added to a final concentration of 330 µM ...
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bioRxiv - Microbiology 2022Quote: ... interleukin-10 (IL-10) and interleukin-4 (IL-4) levels by sandwich ELISA kits (ThermoFisher Scientific). The measurement from unstimulated splenocytes (incubated with medium only ...
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bioRxiv - Immunology 2021Quote: ... 4°C) and cells were spun onto glass slides using a Cytospin 4 Cytocentrifuge (Thermo Scientific), dried for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were migrated on precast Novex 4–20% or 4–12% polyacrylamide gels (Thermo Fisher Scientific), then transferred to Novex nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and then fixed overnight at 4°C in 4% paraformaldehyde (Alfa Aesar, Thermo Fisher Scientific, UK), followed by several washes in 0.1M Phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... diluted in fresh DMEM for 4 h followed by fixation with 4% PFA (Thermo Fisher Scientific). To induce expression of the hGRAD and TRIM21 systems ...
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bioRxiv - Physiology 2023Quote: ... 0.5 mg protein was precleared for 30 minutes at 4°C with 4% Agarose beads (ThermoFisher). To immunoprecipitate RYR1 ...
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bioRxiv - Biochemistry 2024Quote: ... samples were run on Bolt 4–12% SDS-polyacrylamide and native PAGE 4-16% gels (Invitrogen) respectively ...
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bioRxiv - Biochemistry 2022Quote: ... samples were run on Bolt 4–12% SDS-polyacrylamide and native-PAGE 4-16% gels (Invitrogen) respectively ...