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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... RNase OUT (Invitrogen) and MMLV Reverse Transcriptase (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... RNase cocktail (Ambion) was also included in the lysis buffer at a concentration of 5 μl/ml to prevent non-specific interactions mediated by RNA ...
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bioRxiv - Microbiology 2020Quote: ... RNase A (Invitrogen) and 1X cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... RNase A (ThermoFisher), or RNA oligomers (Table 2 ...
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bioRxiv - Microbiology 2020Quote: ... RNase OUT (Invitrogen) and EDTA (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNase I (Ambion) was added to final concentration 0.5 U/µl (replicate 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... RNase H (Ambion) was added to remove the RNA strand of DNA-RNA duplexes ...
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bioRxiv - Pathology 2021Quote: ... RNase I (Ambion) was added to final concentration 2.7 ...
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bioRxiv - Neuroscience 2022Quote: ... RNAse block (Ambion), DIG RNA labelling mix (Roche Applied Science ...
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bioRxiv - Neuroscience 2020Quote: ... RNAse inhibitor (Invitrogen SUPERase-In RNase Inhibitor ...
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bioRxiv - Genomics 2021Quote: ... RNase A (Invitrogen, 1004D was added and the samples were incubated overnight at 65°C ...
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bioRxiv - Microbiology 2023Quote: ... RNase out (Invitrogen), and 1 mM DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... RNase I (Ambion) was added to the extract ...
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bioRxiv - Immunology 2019Quote: ... slides were dipped in water (Invitrogen ultrapure distilled water), air dried and stored at room temperature until they were ablated.
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bioRxiv - Molecular Biology 2021Quote: ... 0.025ug/ul RNAse A (Thermo Scientific RNase A, Cat No EN0531) were incubated at 37°C for 15 minutes ...
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bioRxiv - Genomics 2020Quote: ... RNase Inhibitor (1:40, NEB Murine RNAse Inhibitor or Thermofisher Ribolock) was also added to all lysis buffers and subsequent steps to avoid RNA degradation ...
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bioRxiv - Plant Biology 2021Quote: ... and RNase (0.4 ng μl-1 RNase A; Thermo Fisher Scientific) to degrade extravesicular proteins ...
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bioRxiv - Microbiology 2024Quote: ... and 2 µL RNase A/T1 Mix (4 µg RNase A, 10 U RNase T1, ThermoFisher Scientific, MA, USA) at 37°C for 30 min to remove host-originating nucleic acids ...
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bioRxiv - Molecular Biology 2021Quote: ... The precipitated nucleic acid was washed twice with 75% ethanol and resuspended in nuclease free water before being quantified on a Nanodrop (Thermo Scientific). Approximately 2 µg of AbmR-extracted nucleic acid ...
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bioRxiv - Neuroscience 2019Quote: ... The DNA was eluted in 30 µl of DNAse-free water and concentration measured at 260 nm with an ND1000 Nanodrop spectrophotometer (Thermo Scientific). 125 ng of purified gDNA was used for PCR amplification ...
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bioRxiv - Microbiology 2019Quote: ... DNA was eluted into 100µL of nuclease-free water and quantified using a Qubit® 2.0 fluorometer with the dsDNA Broad Range assay kit (Invitrogen, Singapore). Amplicon libraries were then generated using a modified version of the Illumina 16S Metagenomic Sequencing Library Preparation Protocol22 ...
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bioRxiv - Genomics 2020Quote: ... Beads were subsequently incubated for 45 minutes at 37°C and 1100 rpm in Exonuclease I mix (170 μl nuclease free water, 20 μl 10x Exonuclease I buffer, 10 μl Exonuclease I - Life Technologies). Beads were then washed once in TE-SDS buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The RNA was eluted in nuclease-free water and RNA concentration and quality was measured on a Nanodrop ND-1000 (Thermo Scientific). All RNA samples had a 260/280 ratio above 1.9 ...
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bioRxiv - Plant Biology 2020Quote: ... The lyophilized peptide was dissolved in nuclease-free water and its concentration determined on a Nanodrop 2000c (Thermo Scientific, Waltham, MA) using A280 with the molar extinction coefficient (3230 M-1cm-1 ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... the samples were diluted five times with nuclease-free water and 4 μl of these dilutions were applied for qPCR using the TaqMan Fast Advanced Master Mix (Thermo Scientific).
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bioRxiv - Bioengineering 2020Quote: ... The pellet was resuspended in an appropriate volume of nuclease-free water and the RNA concentration was determined on a Nanodrop (ThermoFisher Scientific). Reverse transcription was performed with 1 μg of RNA template using cDNA Supermix (Quanta Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... The peptides were cleaned using a Sep-Pak Vac C18 cartridge (Waters Corporation) and analyzed label-free by liquid chromatography-tandem mass spectrometry using a Q Exactive mass spectrometer (Thermo Fisher). A 15 cm Å∼ 75 μm C18 column (5 μm particles with 100 Å pore size ...
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bioRxiv - Genomics 2022Quote: ... 2.6 μL nuclease-free water and 5 μL cDNA template on the ABI Prism 7500 Fast Sequence Detection System (Life Technologies). Thermo-cycling conditions were as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was eluted in 50 μl of Nuclease-free water and concentrations were measured using a NanoDrop 1000 (Thermo Fisher Scientific). Integrity of gDNA was analyzed with a 1 % E-Gel™ EX Agarose Gel (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was eluted in nuclease-free water and fluorescently quantified using the Qubit RNA HS Assay (Invitrogen, Waltham, MA, USA), and its quality was evaluated with the RNA 6000 Nano Assay on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Genetics 2019Quote: ... qRT-PCR was performed with the cDNA template diluted 1:4 with nuclease-free water then pipetted into quadruplicate wells of a 384 well optical plate (Cat#4309849 Applied Biosystems). Thermo Maxima SYBR Green/Rox qPCR Master Mix 2X (Cat#K0221 ...
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bioRxiv - Developmental Biology 2019Quote: ... reactions were diluted 5-fold in nuclease-free water and qPCR was performed using taqman multiplex master mix (Thermofisher cat#4461882).
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bioRxiv - Genomics 2019Quote: ... The purified dsDNA samples were then dissolved into nuclease-free water and diluted to 1 ng/µl following Qubit 2.0 measurement (Invitrogen, cat. Q32866). 1 ng of dsDNA ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was diluted 1:5 in nuclease-free water and qPCR reactions were performed in MicroAmp Fast Optical 96-Well Reaction Plates (Applied Biosystems) using PrimeTime qPCR assays (IDT ...
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bioRxiv - Molecular Biology 2019Quote: ... washed twice with 80% ethanol and resuspended in 7 µL nuclease-free water before in vitro transcription at 37°C for 14 hr using the MEGAScript T7 kit (Invitrogen, AM1334). Library preparation was done as described in the CEL-seq protocol with minor adjustments 4 ...
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bioRxiv - Genomics 2021Quote: ... washed twice with 80% ethanol and resuspended in 8 μl of nuclease-free water before in vitro transcription at 37 °C for 14 h using the MEGAScript T7 kit (Invitrogen, AM1334). Library preparation was done as described in the CEL-Seq2 protocol with minor adjustments (Hashimshony et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ng of cDNA was diluted in nuclease-free water and ran in technical triplicates using PowerUp SYBR Green Master Mix (Applied Biosystems) on a QuantStudio 5 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: All primers and probes were diluted to a concentration of 20 μM using nuclease-free water (Ambion, ThermoFisher Scientific, California, USA). Four master mixes for the two multiplex real-time PCR assays (Regional assay and Clade assay ...
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Simultaneous adjunctive treatment of malaria and its co-evolved genetic disorder sickle cell anaemiabioRxiv - Microbiology 2022Quote: ... Serial dilutions of Pf3D7 total RNA and DNA (ranging from 8 to 5000 pg/µl) with nuclease-free water (AM9935, Life Technologies) were used to generate a relative standard curve for pfr1 ...
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bioRxiv - Cell Biology 2023Quote: ... The volume was brought to 250μL with nuclease-free water and RNA was extracted with 25:24:1 Phenol-Chloroform-Isoamyl alcohol (Invitrogen, #15593-031) using a MaXtract tube ...
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bioRxiv - Cell Biology 2022Quote: ... the pellet was dissolved in nuclease-free water and mRNA concentration and purity was determined with a Nanodrop spectrophotometer (Thermo Scientific). 1 µg of mRNA was digested with DNaseI (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was eluted in 30 μL nuclease-free water and used for cDNA conversion with the SuperScript IV Reverse Transcriptase (Thermo Scientific) based on the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cDNA was diluted 1:2 in nuclease-free water and mixed with 500 nM forward and reverse primers and PowerUp SYBR Green Master Mix (Applied Biosystems). qPCR was conducted using an Eppendorf Mastercycler RealPlex4 instrument with the following cycling conditions ...
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bioRxiv - Microbiology 2024Quote: ... IFNβ, IFNα and IFIT1) and 15x (for reference genes: ACTIN, RPL13A, YWHAZ and UBC) with Nuclease-free water (Ambion, Life Technologies). 5 μL of the diluted cDNA was then used for qPCR ...
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bioRxiv - Microbiology 2024Quote: ... The isolated total RNA was eluted in ribonuclease-free water and RNA concentrations were subsequently quantified using a NanoDrop One (Thermo Fisher). cDNA was synthesized from extracted RNA (typically 100 ng ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was then diluted (1:5) with RNAase/DNAase-free water and qRT-PCR performed using SYBR-Green Master Mix (ThermoFisher, #4309155) and primers listed in Supplementary Data ...
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bioRxiv - Microbiology 2020Quote: ... Samples were ethanol precipitated with 20 μg of glycogen and resuspended in 10 μl of RNase free H2O for chemical fragmentation (Ambion Fragmentation Reagent) at 65°C for 4 min ...
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bioRxiv - Cancer Biology 2019Quote: ... The tumors were collected using aseptic technique and the tumors were transferred to RNAse and DNAse free tubes (Thermo Scientific Catalog 374320). Tumors were stored under liquid nitrogen until they were processed for RNA-Seq and WES.
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bioRxiv - Microbiology 2020Quote: ... Supernatants were transferred to a new 1.5 mL RNase-free microcentrifuge tube before proceeding to the washing and elution steps described in the PureLink RNA Mini Kit (Ambion life technologies). Samples were treated with DNase (NEB ...
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bioRxiv - Cancer Biology 2019Quote: ... 14,000 g for 10 min and the supernatant was subjected to Pierce™ Streptavidin Magnetic Beads in the presence of 2% RNase-free BSA (ThermoFisher Scientific) and 2% yeast tRNA (ThermoFisher Scientific ...