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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 4 x TaqMan® Fast Virus 1-Step Master Mix made up with molecular-grade nuclease free water (Ambion) to a final volume of 15 μl ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was resuspended in nuclease-free water and quantified using a spectrophotometer (NanoDrop, Thermo Fisher Scientific, Wilmington, DE, USA). RNA (1 µg ...
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bioRxiv - Immunology 2019Quote: ... 500ng to 2µg of RNA was normalized to the same concentration for each sample using nuclease free water (Ambion) in polypropylene PCR tubes (Starlab) ...
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bioRxiv - Neuroscience 2021Quote: ... 0.252mM CaCl2, 10mM HEPES pH = 7.4, 1mM Pyruvic Acid, 81.7% Mg and Ca free HBSS from Thermofisher, in water) and immediately digested in Cystein-activated Papain solution (17U/ml Papain from Worthington ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was terminated by adding 200 µl of nuclease-free water and 750 µl of TRIzol LS (Invitrogen), and RNA was then isolated and requantified ...
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated by TRIzol-chloroform extraction followed by isopropanol precipitation and finally resuspended in nuclease-free water (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... the sample was drawn off and the grids were rinsed with 5 μL of nuclease-free water (Invitrogen AM9938). The water was then drawn off and the rinse was repeated ...
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bioRxiv - Cancer Biology 2023Quote: ... RNAs were eluted with two rounds of 40 µL of Nuclease-free water (cat no. AM9932, Thermo Fisher Scientific). The quality and size range of each RNA sample was visualized with Bioanalyzer chips ...
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bioRxiv - Neuroscience 2023Quote: ... An aliquot of all amplified cDNA samples was first normalized to 50 pg/ul with Nuclease-Free Water (Ambion), then this normalized sample aliquot was used as input material into the NexteraXT DNA Library Prep (for a total of 50pg) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were filled up to 200 µl with nuclease-free water and 200 µl phenol:chloroform:isoamylalcohol (25:24:1; Thermo Fisher) was added ...
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bioRxiv - Cell Biology 2023Quote: ... Three µL aliquots of diluted samples were lysed by mixing with 3 µL of 0.1% Triton X-100 in Nuclease-Free Water (NFW, cat. #AM9937, Invitrogen) and incubating for 10 minutes at room temperature ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... The RNA was eluted in 30-50 µl Nuclease free water and quantified with Nanodrop 1000 spectrophotometer (ThermoFisher Scientific). RNA quality was assessed using the Qubit RNA IQ assay kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... RNA pellets were dissolved in 50 µl of nuclease-free water and treated with Deoxyribonuclease I according to the manufacturer’s instructions (Invitrogen). The RNA concentration and the purity of the samples were measured with Nano-Drop ND-1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The hippocampi were dissected out and collected in 4.5ml dissection buffer (calcium- and magnesium-free Hank’s BSS: 500 ml distilled water (Gibco, #15230162), 56.8 ml 10xHBSS (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... 150 to 500 ng of total RNA from each sample was treated with RNase-free DNase I (Thermo Fisher Scientific). The reverse transcription reaction was performed with random hexamer primers and RevertAid Reverse Transcritase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The cells were individually collected using an automated micro-manipulating system (CellCelector, ALS) and deposited in nuclease-free microtubes containing 5 μL of 2× CellsDirect buffer and RNase Inhibitor (Invitrogen). Single cell RT-qPCR was then performed using the protocol ADP41 developed by Fluidigm ...
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bioRxiv - Molecular Biology 2021Quote: ... The ribosomal pellets were resuspended in 1 mL of wash A containing 10 U DNase I (RNase-free, Thermo Scientific) and 30 μL BioLock (IBA) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were disrupted with RNase-free zirconia beads and RNA was isolated using Ambion RiboPure RNA Purification kit for yeast (Ambion/ThermoFisher) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2019Quote: ... 2 μg of RNA was incubated with RNase-free DNase (Fermentas) and subjected to reverse transcription using SuperscriptIII SuperMix (ThermoFisher) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... denatured at 65°C for 5 min and treated with 10 U RNase-free DNase I (ThermoFisher Scientific, Waltham, USA) in DNase 1 buffer at 37 °C for 40 min ...
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bioRxiv - Plant Biology 2020Quote: ... was applied to extract total RNA from immature spike tissues followed by removal of genomic DNA contamination using RNAse-free DNAse (Invitrogen). RNA integrity and quantities were analyzed via Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from tissues using the Trizol Plus RNA Purification Kit with DNAse treatment (RNAse-Free DNAse Set, Ambion) and column cleanup (PureLink RNA Mini Kit ...
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bioRxiv - Cell Biology 2021Quote: ... samples were prepared for library preparation by centrifuging the cell suspension at 300 g for 5 minutes at 4°C and then resuspending in 1 mL of cold RNAse-free PBS containing 0.05 U/µL Superase IN (Invitrogen #AM2696) and 0.05 U/µL Enzymatics RNAse Inhibitor (Qiagen #Y9240L ...
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bioRxiv - Cancer Biology 2022Quote: ... The sequences were treated with RNase-free DNase I and purified using a GeneJET RNA Purification Kit (K0731; Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... followed by degradation of both double- and single-stranded DNA from the samples with RNase-Free DNase (Invitrogen, 2028724, USA). Reverse transcription was performed using SuperScriptTM III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was eluted in 30ul RNase-free H2O and concentrations were determined using a NanoDrop ND-100 spectrophotometer (Thermo Scientific). RNA quality was determined by running ∼200-300ng on a 1% agarose gel.
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bioRxiv - Cell Biology 2022Quote: ... The powdered bone tissue samples were transferred into RNAse-free microcentrifuge tubes and RNA was extracted using TRIzol reagent (Cat#. 15596026, Invitrogen), followed by purification using the PureLink RNA mini kit (Cat# ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was eluted in RNAse-free H2O and further purified via treatment with DNA removal kit (ThermoFisher, Cat. No. AM1906). ~1 μg purified RNA was used to generate cDNA via High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... The sample was then homogenized with a RNAse free pellet pestle and RNA extracted using Trizol according to the manufacturer’s instructions (Life Technologies).
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was extracted from cells using the Trizol Plus RNA Purification Kit with DNAse treatment (RNAse-Free DNAse Set, Ambion) and column cleanup (PureLink RNA Mini Kit ...
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bioRxiv - Microbiology 2022Quote: ... Proteinase K solution (20 mg/mL) and RNase (DNase and protease-free, 10 mg/mL) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Developmental Biology 2022Quote: ... Frozen embryos were placed on ice with 500uL chilled 0.1X lysis buffer and homogenized using RNase-Free Disposable Pellet Pestles (Fisher Scientific). Samples were filtered using a 70 µm Flowmi Cell Strainer followed by a 40 µm Flowmi Cell Strainer ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from different banana organs according to a previously described protocol (Asif et al., 2006) and subsequently treated with RNase-free DNase (TURBO DNA-freeTM Kit, Invitrogen). Total RNA was reverse transcribed to generate first-strand cDNA using a RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: All reagents and containers used for RNA processing were RNase-free grade or treated with 0.1% DEPC (4387937, Thermo Fisher) to eliminate RNase contaminants ...
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bioRxiv - Biochemistry 2023Quote: ... 16 mL of HMA-10 buffer was added and the preparation was put on a shaking rocker for one hour at 4 °C with 3 units/mL Rnase-free Dnase (Ambion) added ...
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bioRxiv - Molecular Biology 2023Quote: All reagents and containers used for RNA processing were RNase-free grade or treated with 0.1% DEPC (4387937, Thermo Fisher) to eliminate RNase contaminants ...
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bioRxiv - Genomics 2024Quote: ... 10,000 fibroblasts were processed following the 4f-SAMMY-seq protocol described above but halving the volumes in each step and using 12.5U of RNase– free DNase I (Invitrogen, AM2222); in this way ...
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bioRxiv - Neuroscience 2024Quote: ... The cortex and cerebellum from the left hemisphere was immediately collected in 2mL RNase-free Microfuge Tubes (Thermo Fisher #AM12475) containing 1mL HBSS with no calcium and magnesium (Thermo Fisher #14175095) ...
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bioRxiv - Biochemistry 2021Quote: ... 400 U of RNAse T1 and 10 U RNAse or 400 U RNAse inhibitor (SuperRNAseIn, Invitrogen). The rest of procedure was similar to that employed for RNAse treatment except that the dilution/wash buffer contained 5 mM MgCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.02 U (high RNase treatment) or 0.0025 U (low RNase treatment) of RNase I (Ambion) was added to the lysates ...
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bioRxiv - Plant Biology 2021Quote: ... genomic DNAs were eluted with 50 μl of double-distilled water supplemented with 1 μl of 10 U/μL RNase I (Thermo Fisher Scientific, USA) and stored at −80°C for later experiments.
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bioRxiv - Neuroscience 2022Quote: ... For RNase-resistance assays 100 U RNase I (Thermo Fisher) was added per 100 μg total RNA in clarified lysates ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM MgCl2) supplemented with RNase inhibitor (RNase out, Invitrogen) and Proteinase inhibitor (Complete ...
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bioRxiv - Genetics 2023Quote: ... 80 units of RNAse-OUT RNAse inhibitor (Invitrogen, #15401-029), 0.2% Tween-20 (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 20 U/mL RNase inhibitor RNase Out (Invitrogen), 1 mM PMSF ...
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bioRxiv - Immunology 2021Quote: ... the lentiviral vector (32 µg), the packaging plasmids (VSV-G: 16 µg, gag/pol: 24 µg) and DNAse-free water (Invitrogen) for a final volume of 2 mL ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4.5 µl of RfxCas13d protein (3 mg/ml) were mixed in 1 µl of nuclease-free water with phenol red (Invitrogen). One to 5 nL of RNPs were injected into one- to two-cell stage Ancistrus sp ...
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bioRxiv - Genomics 2019Quote: ... containing 5 μl nuclease-free water with bovine serum albumin (1 mg/μl, Fermentas) and RNaseOut 20 U (Life Technologies). The plates were placed on a precooled rack and stored at −80 °C until analysis ...
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bioRxiv - Genetics 2021Quote: ... cDNA was synthesized by mixing the ezDNase enzyme treated RNA samples in nuclease-free water with SuperScriptTM IV VILO Master Mix (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... nuclease-free water and stored at −20 °C prior to use in real-time Taqman PCR reactions (ThermoFisher, Carlsbad, CA). The resulting cDNA was loaded into a Mouse Inflammation array card (ThermoFisher ...