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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... for each sample was loaded into the wells of a 96 well plate in a volume of 6 µL of nuclease-free water containing 1 U/µL SUPERaseIN (ThermoFisher). After adding 1.5 µL of 10 µM barcoded RT primer (Integrated DNA Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... nCOV-N1 FAM or nCOV-N2 FAM primer and probe mix (10006713, IDT) and nuclease-free water (1907076, Thermo Fisher) and 5 μL of sample prepared by extraction or SwabExpress was added to each well ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was ethanol precipitated in the presence of 20 μg of glycogen and resuspended in 50 μL of nuclease-free water (Ambion) in a DNA low-bind tube ...
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bioRxiv - Plant Biology 2020Quote: ... DNA was ethanol precipitated in the presence of 20 μg of glycogen and resuspended in 50 μL of nuclease-free water (Ambion) in a DNA low-bind tube ...
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bioRxiv - Evolutionary Biology 2021Quote: 10 μg of total RNA per sample were diluted in 15 μL nuclease free pure water (Applied Biosystems/Ambion, Austin) and mixed with 10 μL sample buffer (50% formamide ...
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bioRxiv - Evolutionary Biology 2021Quote: 10 μg of total RNA per sample were diluted in 15 μL nuclease free pure water (Applied Biosystems/Ambion, Austin) and mixed with 10 μL sample buffer (50% formamide ...
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bioRxiv - Neuroscience 2021Quote: ... 1mg of NEP1-40 was diluted in 21.62µL of DMSO and 843.2µL of nuclease-free water (Thermo Fisher Scientific, cat# AM9937) to a working concentration of 250µM ...
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bioRxiv - Molecular Biology 2020Quote: ... Extracted RNA was re-suspended in 30 ul of nuclease-free water and quantified on the NanoDrop spectrophotometer (Thermo Scientific). cDNA synthesis was carried out with 500 ng of RNA using the SensiFAST cDNA Synthesis Kit (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... with minor alterations including no vigorous mixing steps and final elution in 100μl of nuclease free water and quantified using a Qubit and the HS (high sensitivity) dsDNA assay kit (Thermofisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... with the exception of OCT3/4 and Nanog which were taken from Chen et al 201735) were diluted in PCR grade nuclease-free water (Gibco) at a concentration of 100 µM ...
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker and then incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Immunology 2020Quote: ... Samples were eluted with 30 μl nuclease-free water (DEPC) into 96-well plates and quantify by Qubit Fluorometric Quantitation assay (Invitrogen).
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bioRxiv - Evolutionary Biology 2022Quote: ... Total RNA was eluted in 50 μl of nuclease free water and quality was checked using nanodrop before quantification using the Qubit RNA HS assay kit (ThermoFisher). The library preparation and sequencing were performed at the Genomics Core Facility (Department of Clinical and Molecular Medicine ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in nuclease free water and the concentration was measured using a NanoDrop 2000 spectrophotometer (Thermo Scientific). The extracted RNA was treated with ezDNase Enzyme (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: The A260/230 of all total cell RNA samples dissolved in nuclease-free water were determined to be above 1.7 by Nanodrop (ThermoFisher, ND2000CLAPTOP) before proceeding to cDNA synthesis ...
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bioRxiv - Physiology 2022Quote: ... RNA was eluted in RNAase-free water and quantified using a NanoDrop 2000 Spectrophotometer (Fisher Scientific Company, Ottawa, ON, Canada).
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bioRxiv - Molecular Biology 2023Quote: ... RNA was eluted in 100 µL of nuclease free water and precipitated at -80 °C for ≥ 3 hours with 1 µL of 15 mg/mL GlycoBlue coprecipitant (ThermoFisher), 10 µL of 3 M sodium acetate pH 5.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The dilutions were mixed with an equivalent volume of 400 nM DREmut or DREwt (Figure S4; SI 1A) in nuclease-free water (Ambion) and incubated for 20 minutes at 25 °C followed by 10 minutes at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... 500ng of mRNA were diluted to 20ul of nuclease-free water then 40ul of 2 x RNA loading dye from ThermoFisher Scientific (Cat# R0641 ...
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bioRxiv - Biophysics 2022Quote: ... the 5’-cy5 labeled TS ssDNA were synthesized by Sangon Biotech and diluted to 10 μM in nuclease-free water (Invitrogen). dsDNA was prepared by mixing 5’-cy5 labeled TS and unlabeled complementary oligo at the molar ratio of 1:1.2 in annealing buffer (10 mM HEPES-Na pH 7.5 ...
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bioRxiv - Systems Biology 2024Quote: ... The double-stranded cDNA was eluted into 15 µl nuclease-free water and concentration measured using a Qubit Fluorometer 3.0 with dsDNA high-sensitivity assay kit (ThermoFisher Q32851).
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bioRxiv - Microbiology 2023Quote: ... 0.25 µM of each primer and 2 µl of DNA template in a final volume of 40 µl with nuclease free water (Ambion, Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2023Quote: ... Samples were eluted in 30 µL nuclease-free water into 96-well plates and quantified by Qubit Fluorometric Quantitation assay (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were subsequently subjected to FCCS and treated with 100 μg/ml RNase A (DNase and protease-free, EN0531, Thermo Scientific) when indicated ...
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bioRxiv - Microbiology 2022Quote: ... RNA was eluted in 10μl H2O containing 2U RNase inhibitors and a second DNase step was performed for 30 minutes using the Turbo DNA-Free kit (Invitrogen AM1907M) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were disrupted with RNase-free zirconia beads and RNA was isolated using Ambion RiboPure RNA Purification kit for yeast (Ambion/ThermoFisher) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... All the individual RNA preparations were subjected to DNase digestion by RNase free DNase I (Thermo Fisher Scientific, Waltham, MA, USA) and purified using RNeasy MinElute Cleanup Kit (Qiagen ...
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bioRxiv - Biophysics 2020Quote: ... RNC samples purified from sucrose cushions as described above were treated with 100 μg mL-1 DNase-free RNase A (ThermoFisher, EN0531) at 37 °C for 50 min followed by western blot with monoclonal Anti-FLAG M2-Peroxidase (HRP ...
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bioRxiv - Pathology 2022Quote: ... The contaminating DNA was eliminated using RNase-free DNase and the first-strand cDNA was synthesized using Revert Aid First Strand cDNA Synthesis Kit (Thermo Fisher). Quantitative RT-PCR analysis was performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech ...
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bioRxiv - Genetics 2022Quote: ... samples were blocked in RNase free blocking solution (0.3% v/v PBT, 0.5% ultra-pure Bovine Serum Albumin) (Ambion, cat. no. AM2616) for 45 min and primary and secondary antibody stainings were carried out ...
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bioRxiv - Microbiology 2022Quote: ... For qRT-PCR experiments 1 µg of RNase-free DNase-treated RNA was reverse transcribed with random hexamer primers using RevertAid first strand cDNA Synthesis Kit (Thermo Scientific). All qRT-PCR reactions were performed in triplicate in a 20 µl mixture containing cDNA (5 µl of 1:20 dilution) ...
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bioRxiv - Neuroscience 2020Quote: ... in a RNase/DNA-free fume hood 2 µl of RNA was mixed with 1 µl of dNTP mix (Thermo Scientific), 1 µl of Oligo-dT primer (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... treated and untreated spinal cord injured CX3CR1+/GFP mice were anesthetized (tribromoethanol, 500mg/kg) and intracardially perfused with 0.1M RNAse-free phosphate base saline (PBS, Invitrogen, Carlsbad, USA). Spinal cords were dissociated in an enzymatic cocktail [750µl PBS ...
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bioRxiv - Microbiology 2020Quote: ... Each sample was aliquoted (2×250 mL) into sterile RNase-free Nunc tubes (cat. no. 376814; Thermo Fisher Scientific, Waltham, MA). Particle-associated communities on magnetic alginate (AlgP ...
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bioRxiv - Genetics 2022Quote: Cells were fixed in 70% ethanol overnight and then treated with 0.25 mg/ml DNase- and Protease-free RNase A (ThermoFisher Scientific, 10753721) at 37°C for 2h and Proteinase K (Biofroxx ...
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bioRxiv - Immunology 2022Quote: ... The blood was collected by cardiac puncture and placed into a 1.5mL Eppendorf tube with 5mM EDTA (pH 8.0, RNase-free; ThermoFisher Scientific, Cat# AM9261). For the PLNs and spleen ...
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bioRxiv - Synthetic Biology 2022Quote: ... according to the manufacturer’s instructions and the extracted RNA was DNAse treated with Invitrogen RNase-free DNAse (Thermo Fisher Scientific, USA). Sequencing library preparation was then performed using Stranded Total RNA Prep Ligation kit with Ribo-Zero Plus for rRNA depletion and by using 10 bp IDT for Illumina indices (all from Illumina ...
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bioRxiv - Microbiology 2023Quote: ... On-column DNA digestion was conducted by adding 80 μl (10 μl 1 Unit/μL RNase free DNase I, (ThermoFisher Scientific) in 70 μl reaction buffer from the Master Pure Yeast RNA Purification kit ...
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bioRxiv - Immunology 2023Quote: ... For collection of ‘free chromatin’ 30 μg of sonicated samples was treated with 10 μg/μl of RNase A (Thermo Scientific) in the presence of 0.2 M NaCl at 37°C for 1.5 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were first collected in RNase-free PBS and then mounted on slides (Tissue Path Superfrost Plus Gold Microscope Slides, Fisher Scientific). After air-drying at room temperature for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... 20μl 5x Laemmli buffer was added and lysis was performed by grinding tissue with plastic pestles (RNase-free disposable pellet pestles; Thermo Scientific). Samples were boiled for 10 min and briefly spun down before loading ...
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bioRxiv - Genetics 2023Quote: ... RNA was extracted according to the manufacturer’s instructions and residual DNA in the homogenized samples was removed by RNAse free DNase I (Thermo Fisher Scientific). Quality of RNA samples were checked with Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2023Quote: ... The cells were then pelleted into a RNase-free Eppendorf tubes and were lysed in 1ml Trizol reagent (Invitrogen, Ref. 15596026). 10 minutes after the addition of Trizol ...
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bioRxiv - Neuroscience 2023Quote: ... The aqueous phase containing total RNA was transferred into a fresh RNAse-free tube and cleaned using the PicoPure Kit (ThermoFisher KIT0204) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The aqueous phase containing the RNA was transferred to a new tube and 5µg of RNase-free Glycogen (Thermo Fisher Scientific) have been added ...
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bioRxiv - Genomics 2024Quote: ... They were processed following the 4f-SAMMY-seq protocol described above but using only 2U of RNase–free DNase I (Invitrogen, AM2222).
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bioRxiv - Genomics 2024Quote: ... with protease inhibitors) and incubated for 60 min at 37°C with 25 U of RNase–free DNase I (Invitrogen, AM2222). To stop DNA digestion ...
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bioRxiv - Microbiology 2024Quote: ... DNase treatment was performed on the lysates by adding 1µL of RNase-free DNase I at 1U/µL (Thermo Fisher Scientific), 10mM MgCl2 ...
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bioRxiv - Molecular Biology 2019Quote: ... RNase inhibitor (ThermoFisher). Protein concentrations were determined using BCA Protein assay Kit (Thermo Scientific Pierce) ...
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bioRxiv - Molecular Biology 2020Quote: ... RNase I (Invitrogen) 50 U/mg of extract was used at 37 °C for 5 minutes ...