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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... RNase H (Invitrogen; 18021071) and 5 x second-strand buffer were replaced with E ...
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bioRxiv - Genetics 2022Quote: ... RNase A (Ambion, USA) and Benzonase (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... and RNAse inhibitor (ThermoFisher) using a hand pestle ...
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bioRxiv - Genomics 2022Quote: ... RNase A (Ambion, USA) and Benzonase (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNase OUT (Invitrogen). The RNA template was removed after cDNA synthesis by incubating for 20 minutes at 37°C with 10 units RNase H (New England Biolab) ...
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bioRxiv - Microbiology 2023Quote: ... RNAse T1 (Thermo Scientific), RNase A (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... RNase A (Thermo Scientific) for 3 hours at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNase inhibitor (Invitrogen)] ...
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bioRxiv - Systems Biology 2023Quote: ... RNAse I (Invitrogen, AM2294) was added to 0.4U/μg of RNA and incubated at 25 °C for 45 minutes ...
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bioRxiv - Microbiology 2023Quote: ... RNase-Out (Invitrogen, USA) and random nonamer primers ...
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bioRxiv - Plant Biology 2023Quote: ... RNase T1 (Thermo Scientific) was included as a positive control in the assay.
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bioRxiv - Immunology 2023Quote: ... RNase inhibitor (Applied Biosystems)] for 30 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and RNase inhibitor (Invitrogen) as previously described (30) ...
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bioRxiv - Bioengineering 2024Quote: ... RNase H (ThermoFisher Scientific) was used to remove RNA template from the cDNA following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... containing RNAse OUT (ThermoFisher) for 45 and 40 min ...
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bioRxiv - Molecular Biology 2024Quote: ... RNase I (Ambion, AM2294) was added to the lysate ...
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bioRxiv - Cancer Biology 2024Quote: ... 2ul RNase A (Invitrogen), and 3.5ul of 1M MgCl2 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... RNAse inhibitor (Ambion AM2696)) and scraping cells into a 15ml conical tube ...
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bioRxiv - Immunology 2024Quote: ... with RNase Inhibitor (Invitrogen). qRT-PCR was performed with 20 ng of cDNA with PowerUpTM SYBRTM Green Master Mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2024Quote: ... coli RNase H (Invitrogen), E ...
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bioRxiv - Genomics 2021Quote: ... Elution was performed in nuclease-free water and 1 μL was used for measuring the concentration with the Qubit™ dsDNA High Sensitivity Assay Kit (ThermoFisher Scientific), while the size was assessed with a High Sensitivity DNA chip on an 2100 Bioanalyzer.
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bioRxiv - Neuroscience 2021Quote: ... total nucleic acids were resuspended in 50 μL ultrapure water and treated with DNAse I (Turbo DNA Free® kit; AM1907, Ambion®) to eliminate any trace of possible genomic DNA contamination ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was stored at -80°C in nuclease-free water and concentration was determined with a Nanodrop 1000 instrument (Thermo Fisher Scientific). Samples were sent to a sequencing facility (Novogene Corporation Inc. ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNAs and DNAs were dissolved in nuclease-free water and their concentration and purity established by spectrophotometry (Nanodrop 1000, Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1-10 ng template in 9 μl Nuclease-free water were added to 10.0 μL of 2× TaqMan® Universal PCR master mix (ABI/Life technologies, USA) and 1.0 μL of a 20× combined primers and probes mix (ABI/Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified RNA was eluted in 40 μl of nuclease-free water and then was quantified using a Nanodrop spectrophotometer (Thermo Fisher Scientific). For analysis of tethered function MS2 reporter mRNAs by RT-qPCR ...
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bioRxiv - Genomics 2022Quote: Dried RNA from stage embryos was resuspended in 9 µL nuclease-free water and 1 µL used for quantification by NanoDrop™ One/OneC spectrophotometer (Thermo Scientific). The remaining RNA (< 3 µg ...
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bioRxiv - Physiology 2022Quote: ... Precipitated RNA was resuspended in nuclease free water and 1 µg total RNA was reverse transcribed into cDNA using SuperScript™ II (Invitrogen, UK) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was eluted in 30 μl nuclease-free water and quantified using a NanoDrop ND-1000 microvolume spectrophotometer (ThermoFisher Scientific, Waltham, MA). cDNA was generated from 150 ng of RNA in a 20 μl reaction using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... The gel was imaged and washed 2 x 15 minutes in nuclease-free water then 2 x 15 minutes in 10X UltraPure SSC Buffer (Invitrogen, Carlsbad, CA) with gentle shaking ...
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bioRxiv - Cell Biology 2022Quote: ... the pellet was dissolved in 20-50 µl of molecular grade RNAase-free water and stored overnight at -20°C before the concentration and purity were checked using the Nanodrop OneC (Thermo Fisher Scientific). cDNA was synthesized using 5 µg RNA as a template per reaction with the Superscript III First Strand Synthesis System Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The oligos were annealed in a thermocycler with gradual T reduction from 95°C to 25°C at a rate of 5°C/min and subsequently diluted 1:20 into Nuclease free water (ThermoFisher Scientific AM9938). The pLentiCRISPRv2 plasmid was digested for 1 hr at 55°C with 1U per μg of DNA BsmBI-v2 (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was dissolved in RNAse-free water and 1 µg estimated using Nanodrop was used to make 20 µl cDNA (Verso cDNA synthesis kit, Thermo Fisher Scientific). cDNA was amplified using the PowerUp SYBR Green master mix (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... 100 ng total RNA from each sample was diluted to 15 µl using nuclease- free water and transferred to 96-well plates (Applied Biosystems N8010560). We added 1 µl of 1:1,000 dilution of ERCC Ex-Fold Spike-Ins (ThermoFisher 4456739 ...
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bioRxiv - Neuroscience 2023Quote: The synthetic RNA was diluted in nuclease free water and the following concentrations were confirmed with Qubit RNA Broad Range Assay Kit (Thermo Fisher Scientific): 10 ng/ul ...
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bioRxiv - Microbiology 2024Quote: ... Samples were eluted in 30 μL nuclease-free water pre-warmed at 50°C and quantified by NanoDrop One (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... cDNA was subsequently diluted 3x (for target genes: IFNβ, IFNα and IFIT1) and 15x (for reference genes: ACTIN, RPL13A, YWHAZ and UBC) with Nuclease-free water (Ambion, Life Technologies). 5 μL of the diluted cDNA was then used for qPCR ...
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bioRxiv - Developmental Biology 2021Quote: ... Individual plates were harvested using UV-sterilized OCPs and gastruloids were pooled into a 15ml centrifuge tube using RNase-free wide-bore P1000 pipette tips (Thermo Fisher Scientific, 2079G). When all plates from a given condition were harvested ...
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bioRxiv - Neuroscience 2021Quote: ... we reverse transcribed RNA into cDNA in an RNAse free environment using the RT TaqMan® MicroRNA Reverse Transcription (RT) kit (Applied Biosystems, ABI) with the miRNA-specific commercial primers (ABI ...
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bioRxiv - Cancer Biology 2020Quote: ... Eluted samples were incubated at 37°C for 30 mins with 1 μL of DNase and protease-free RNase A (10 mg/mL, Thermo Fisher Scientific EN0531). DNA and protein cross-linking was reversed by adding 8 μL of 5M NaCL and 2 μL of Proteinase K Solution (20 mg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... all samples were purified using AmpureXP beads at a volumetric ratio of 0.5X with a final elution in 13 µl of H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049). The cDNA was then quantified using the Qubit High Sensitivity dsDNA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A large-bore pipette filled with a low amount (approximately 1 µL) of RNase-free phosphate buffered saline (PBS) (Invitrogen, Carlsbad, CA, USA) was used to aspirate the same cell under visual control ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was diluted in RNase-free H2O (range 20-100 μl) and quantified on a Nanodrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). Subsequently ...
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bioRxiv - Systems Biology 2023Quote: ... The cell lysates were purified according to the kit’s protocol titled “purification of total RNA from yeast.” Extracted RNA was then treated with Turbo RNase-free DNase kit (ThermoFisher, Waltham, MA, USA) according to the manual and purified again with the RNeasy mini kit protocol titled “RNA clean up.” The stranded RNAseq libraries were prepared with Illumina’s TruSeq Stranded mRNA Sample Prep kit ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μL ribodepleted RNA was mixed with 4 μL 5× TGIRT buffer (2.25 M NaCl, 25 mM MgCl2, 100 mM Tris-HCl, pH 7.5; Ambion RNase-free Buffer Kit AM9010), and 1 μL random hexamer primers (xGen) ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was stopped by three washes with PBS and incubated with FBS-free medium containing 0.2 mg/mL RNase A (Thermo Fisher Scientific, Cat# 12091021) for 1 hours to digest the extracellularly attached RNAs ...
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bioRxiv - Genomics 2019Quote: ... 5 μl of RNase A/T1 Mix (RNase A at 500 U/mL and RNase T1 at 20000 U/mL, Ambion AM2286) diluted 100 times were added to each tube ...
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bioRxiv - Molecular Biology 2021Quote: ... the total protein extract was supplemented with 20 µg/mL RNAse A and 50 U/mL of RNAse T1 (RNAse A/T1 Mix, Thermofisher Scientific) and incubated 10 min at 37°C.
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bioRxiv - Molecular Biology 2022Quote: ... supplementing with 1 μl of RNase A/T mix (2 mg/ml of RNase A and 5,000 U/ml of RNase T1, Thermo Fisher Scientific), and incubating the resulting mixtures for 20 min at 37°C.
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bioRxiv - Microbiology 2021Quote: ... water (LC-MS grade water from Thermo Fisher Scientific, Waltham, MA). Cell pellets were flash frozen in liquid nitrogen and stored at −80°C ...