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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and RNAse inhibitors (RNase Inhibitor (Thermo Fisher, Carlsbad ...
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bioRxiv - Cell Biology 2021Quote: The cell culture supernatant was mixed with an equal volume of 2×RNA lysis buffer (distilled water containing 0.4 U/uL SUPERase ITM RNase Inhibitor (Thermo Fisher Scientific), 2% Triton X-100 ...
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bioRxiv - Genomics 2023Quote: ... 1 mL of water was added to dilute the SDS and the samples were incubated with 5 μL of RNAse A (ThermoFisher, EN0531) at 37 °C for 2 hours and with 8 μL of proteinase K (Euromedex ...
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bioRxiv - Genomics 2023Quote: ... remaining RNA was digested with RNase mix (0.25U/μL RNase H, 2.5% Invitrogen RNase cocktail mix, 1x RNase H buffer) at 37 °C for 1 hour followed by two PBST washes ...
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bioRxiv - Microbiology 2019Quote: ... Samples were eluted in 40 μL nuclease-free water into MicroAmp Optical 96-well reaction plates (Applied Biosystems). RNA can be covered and stored at −80°C or used directly for reverse transcription.
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bioRxiv - Neuroscience 2019Quote: ... Precision PLUS Mastermix StepOnePlus and nuclease-free water was incubated in the Real-Time PCR instrument (Applied Biosystems) using the following conditions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Products were eluted with 16 μl nuclease-free water and concentration was measured with NanoDrop (Thermo Fisher Scientific).
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bioRxiv - Immunology 2019Quote: ... was diluted to 1x with sterile nuclease-free water and supplemented with 15 mM HEPES pH 7.4 (Gibco) to prepare HBSS-HEPES while it was supplemented with 2mM EDTA ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR product was purified with 0.85:1 ratio by VAHTS DNA Clean Beads (Vazyme, Cat.No.N411) and eluted in 30μl nuclease-free water (Invitrogen, Cat.No.AM9937) for another 18 cycles of PCR ...
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bioRxiv - Synthetic Biology 2020Quote: ... 2 μL of cell-free reaction product was diluted into 48 μL of Ambion nanopure water (Invitrogen, USA). The solution was then placed in a Costar 96-well black assay plate (Corning ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were concentrated in an Amicon Ultra 7 kDa filter and buffered exchanged into nuclease free water (Invitrogen), to which the indicated buffer and salt concentrations were added ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng mRNA was diluted in a total volume of 14 ml nuclease-free water (Thermo Fisher Scientific) and digested by nuclease P1 (1 U ...
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bioRxiv - Microbiology 2022Quote: ... Ten μg from the total RNA samples were diluted to 20μl in nuclease-free water (UltraPure™, Invitrogen) and then they were denatured at 60°C for 1min ...
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bioRxiv - Microbiology 2023Quote: ... primers at concentrations listed in supplemental table 1 and DNA/RNAase free water (Fisher Scientific, Cat. No. 10977015) to make the remaining volume ...
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bioRxiv - Genomics 2023Quote: ... Sequence-verified plasmids were purified with the ZymoPure Maxiprep kit (ZymoResearch) and eluted in nuclease-free water (Invitrogen).
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bioRxiv - Biochemistry 2023Quote: ... and the pellets were washed three times with 70% EtOH and eluted in Nuclease-free distilled water (Invitrogen). From the RNA samples ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was dissolved in nuclease-free water and RNA concentration was measured using a Nanodrop (Thermo Scientific). RNA integrity and quality were estimated using a Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... which were then washed three times with 70% EtOH and eluted in Nuclease-free distilled water (Invitrogen, #10977015).
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bioRxiv - Physiology 2020Quote: ... with additional RNase inhibitor (Invitrogen RNase OUT, 10777019). cDNA was quantified using a ViiA 7 (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the extracted RNA samples were run on standard RNase-free 1.0% agarose gels and measured using a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... qPCRs were done by mixing 3.25 μL of H2O RNAse free from the RNA extraction kit to 6.25 μL of Fast SYBR green Master mix (ThermoFisher) and 0.25 μL of reverse and 0.25 μL of forward primers (10 pM ...
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bioRxiv - Genomics 2021Quote: ... then the pellet was resuspended in 800 µl NST buffer containing 0.1% DNase free RNase A (Thermo Scientific, EN0531), and 10% fetal bovine serum (Life Technologies,10108-105) ...
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bioRxiv - Cell Biology 2022Quote: ... Remnant villi were removed by shaking tissue pieces at 4°C in an initial 5 minute incubation PBS with 10 mM RNase-free EDTA pH 8.0 (Invitrogen). Crypts were collected and filtered through 70 µm cell strainers in subsequent fractions ...
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bioRxiv - Neuroscience 2022Quote: ... and collected in an RNase free 1.5 mL Eppendorf tube in 1 ml TRIzol solution (Thermofisher Cat. No. 15596026). Nerves were minced into small pieces with RNase free spring scissors ...
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bioRxiv - Cell Biology 2019Quote: ... The beads were then washed with 1.45 µl RNAse-free bind and wash solution containing 0.01 mM Tris (Invitrogen, AM9855G), 1mM EDTA (AM9260G) ...
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bioRxiv - Neuroscience 2020Quote: ... the cytosol of the recorded cell was aspirated into the patch pipette and immediately expelled into an RNase-free 0.5-ml tube (Ambion). Library Preparation and mRNA Sequencing ...
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bioRxiv - Biophysics 2020Quote: ... Nuclei was stained by first placing the scaffolds in 10% RNAse in PBS (DNase and protease-free) (Thermo Fisher) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... A glass needle (Garner Glass Company) was front-loaded with ∼1 µl RNase free 0.01M PBS (Thermo Fisher Scientific), contacted to the identified Aii-ACs ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 µL of RNA sample (1.0-1.9 µg) were digested with 2 units of RNase-free DNase (Ambion/Invitrogen) in 10 µL of RNase-free DNase buffer at 37°C for 30 min ...
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bioRxiv - Genomics 2022Quote: ... RNA mixtures were prepared on ice by combining 4.25 μl of nuclease free H2O with 1 μl of Superase•In RNase inhibitor (AM2696, ThermoFisher), mixing ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was homogenized with a RNase-free glass 0.5mL Dounce and RNA was extracted with Trizol following manufacturer instructions (Invitrogen). 500ng of RNA were retrotranscribed (Quantiscript ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 0.5mg of tissue was lysed in lysis buffer and total RNA was isolated and eluted from a column with 50μL RNase-free elution buffer and further treated with Turbo DNase (Ambion). RNA (500ng ...
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bioRxiv - Neuroscience 2023Quote: ... The metal specimen tray was filled with pre-chilled 1X RNase free PBS (phosphate buffered saline, ThermoFisher Scientific, #AM9625). Coronal slices measuring 250 uM in thickness were cut from rostral to caudal at slow speed (0.07mm/s ...
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bioRxiv - Developmental Biology 2023Quote: ... 1μg of each RNA sample was incubated with 1U of RNase-free DNase I (Thermo Fisher Scientific, Cat# 89836) for 30 minutes at 37°C to eliminate residual DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... eluted with 40μl RNase-free ddH2O and the RNA concentration was measured with a Nanodrop 2000c spectrophotometer (Thermo Scientific). RNA samples were stored at -70°C ...
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bioRxiv - Biophysics 2021Quote: ... RNase (Invitrogen) and MgCl2 and mixed in rocking platform at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... RNase (Invitrogen) and 10 mM MgCl2 and kept at 4°C in rocking platform ...
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bioRxiv - Microbiology 2022Quote: ... RNase Cocktail (500 Uml-1 RNase A, 20.000 Uml-1 RNase T1) (Thermo Fisher, USA), 0.05 M EDTA pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... buffer + RNase Cocktail enzyme mix (RNase A + RNase T1) (cat no. AM2286, Thermo Fisher Scientific) (Final concentration RNAse A 5 units and RNase T1 200 Units) ...
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bioRxiv - Plant Biology 2024Quote: ... The pellet was washed with 75% ethanol and gently resuspended in sterile water and treated with RNAse A (Thermo Scientific™ EN0531) and Ribonuclease T1 (Thermo Scientific™ EN0541 ...
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bioRxiv - Microbiology 2020Quote: ... RNAse T1 and RNAse A (all: Thermo Fisher Scientific) for 30 min at 37°C prior to infection.
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bioRxiv - Immunology 2022Quote: ... RNAse treatment was carried out by RNAse cocktail (Invitrogen). Quantitative assessment of DNA was performed by a Nanodrop 2000 spectrophotometer and DNA integrity was confirmed by gel electrophoresis ...
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bioRxiv - Biochemistry 2023Quote: ... RNase A and RNase H were purchased from ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA samples were diluted 1:10 with nuclease-free water (Life Technologies Corp., Austin, TX, USA, Cat# AM9937) Real-time quantitative PCR (qPCR ...
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bioRxiv - Physiology 2019Quote: ... After washing the bead/RNA pellets with 80% ethanol the sgRNAs were eluted in nuclease free water (Ambion, AM9937) and stored at −80 °C until use.
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bioRxiv - Evolutionary Biology 2021Quote: ... washed and re-suspended in 40 μL of 50% formamide diluted in nuclease free water (Applied Biosystems / Ambion, Austin).
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bioRxiv - Developmental Biology 2020Quote: ... A volume of 300μL nuclease-free water and 400μL phenol-chlorophororm-isoamyl (PCI) (25:24:1) was added (Invitrogen 15593031) to the samples in a phase-lock tube ...
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bioRxiv - Evolutionary Biology 2021Quote: ... washed and re-suspended in 40 μL of 50% formamide diluted in nuclease free water (Applied Biosystems / Ambion, Austin).
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bioRxiv - Molecular Biology 2021Quote: ... 50 μl cDNA template in nuclease-free water and 50 μl of TaqMan Universal PCR Master Mix (Applied Biosystems) were mixed and added to each TLDA loading port ...
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bioRxiv - Cell Biology 2022Quote: ... The RNA was eluted with 60 μl of nuclease-free water and measured spectrophotometrically with Nanodrop (Thermo Fisher Scientific). cDNA was synthesized using the reverse transcription system (Promega ...