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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 0.12 U μl−1 Turbo DNase (Albion) or 5 U RNase A and 20 U RNase T1 (RNase Cocktail enzyme mix, Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were then cooled and digested with 5 μL RNase A/T1 mix (2 mg/mL of RNase A and 5,000 U/mL of RNase T1, Thermo Fisher, cat#EN0551) overnight at 37 ℃ ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... 500 mM KCl, 7.5% w/v RNAse free sucrose, 2 mM MgCl2, 75 mM NH4Cl, 2 mM puromycin [Fisher Scientific], and 2 mM DTT) by gentle pipetting ...
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bioRxiv - Microbiology 2024Quote: ... ssRNA/dsRNA fractionation was performed as previously described 48. To visualize dsRNA, 1 µg were pretreated with RNase-free DNase1 (RBC Bioscience, cat. #9680DN050) and S1 nuclease (Thermo Scientific™, cat. #EN0321) according to the manufacturer’s instructions and electrophoresed on 1% agarose gel stained with ethidium bromide.
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bioRxiv - Developmental Biology 2023Quote: ... Pooled ROs (4-5 ROs for each replicate) were washed in RNase-free PBS-/- and incubated at 37°C in fresh 1X TrypLE (Thermo Fisher Scientific, catalog #12563029) for 20 minutes.
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bioRxiv - Microbiology 2021Quote: ... Total DNA was eluted in nuclease-free water and quantified using the dsDNA-HS on a QubitTM 3.0 fluorometer (Thermo Fisher Scientific, Waltham, MA). The 16S rRNA V4 hypervariable region was amplified from DNA using the barcoded 515F forward primer and the 806Rb reverse primers from the Earth Microbiome Project (81) ...
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bioRxiv - Microbiology 2020Quote: ... DNA was purified by phenol-chloroform extraction followed by isopropanol-sodium acetate precipitation and then resuspended in 50 μl nuclease-free water (Thermo Fisher, #10977-023). Extracted DNA was further diluted to 10 ng/ul and subjected to qPCR targeting of the EBV BALF5 gene ...
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bioRxiv - Pathology 2021Quote: ... For each tumor sample 1000 ng of cDNA was diluted to 55 uL with nuclease-free water and 55 uL of TaqMan gene expression master mix was added (Life Technologies, cat # 4352042). A total of 100 uL of sample was added to each of the 8 ports on a single card ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were eluted into 14 μl nuclease-free water and the RNA concentration was measured using Qubit HS RNA assay (Thermo Fisher Scientific #Q32852). Samples were stored at −20°C ...
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bioRxiv - Genomics 2022Quote: ... DNA ligation products were resuspended in 130μl of nuclease free water and concentration was assessed by fluorimetric quantification using the Qubit dsDNA HS Assay Kit (Thermo Fisher cat. #Q32851).
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bioRxiv - Immunology 2022Quote: ... The cDNA preparation was then pre-diluted 1:5 with RNAase-free water and quantified using the “TaqMan© qPCR analysis TaqMan Universal Master Mix II” (Invitrogen, Cat# 4427788). For each reaction ...
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bioRxiv - Neuroscience 2022Quote: ... RNA samples were resuspended in a final volume of 12 μl of nuclease-free water and quantified by absorbance at 260 nm in a spectrophotometer (NanoDrop 2000, Thermo Fisher Scientific, USA). One microgram of total DNase-treated RNA was reverse-transcribed in a 20-μl reaction volume using M-MLV (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and 1mL of ethanol solution (80% ethanol in DNAse free water) were added to specified wells in a KF Duo Deepwell 96 plate (Thermo Fisher Scientific, MA) and loaded on the KingFisher Duo Prime Purification System ...
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bioRxiv - Bioengineering 2023Quote: ... 500 ng of cDNA were combined into a solution with 55 μL of nuclease free water and 55 μL TaqMan Universal PCR Master Mix (Thermo Fisher cat. 4305719). The combined solution was loaded into the gene array cards ...
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bioRxiv - Genomics 2023Quote: ... leaving behind a single-stranded DNA (ssDNA) which was then purified via magnetic bead purification and eluted in nuclease-free water (Ambion, cat. no. AM9930). The primers used for PCR are as follows:
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bioRxiv - Developmental Biology 2023Quote: ... Synthesized mRNA was purified by LiCl precipitation then dissolved in nuclease-free water and quantified by NanoDrop spectrophotometer (Thermo Fisher Scientific, ND-LITE) and stored at −80°C.
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bioRxiv - Cancer Biology 2023Quote: ... The cDNA products were diluted (1:10) with nuclease-free water and analyzed by qPCR using Power SYBR Green PCR Master Mix (Applied Biosystems, cat.#4368708) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were diluted in nuclease free water and used for qRT-PCR reactions with the SYBR® Green PCR master mix (Applied Biosystems, www.appliedbiosystems.com) using an Applied Biosystems 7900HT Instrument ...
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bioRxiv - Biochemistry 2024Quote: ... was performed on the cDNA (diluted 1 in 5 in nuclease-free water) using the Power SYBR Green PCR Master Mix and the StepOnePlus Real-Time PCR System (both Applied Biosystems, Warrington, UK). MALAT1 expression was normalised to RPL10 (60S ribosomal protein L10 ...
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bioRxiv - Plant Biology 2021Quote: ... vesicles were treated with RNase (0.4 ng μl-1 RNase A; Thermo Fisher Scientific) to remove extravesicular RNA before RNA isolation ...
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bioRxiv - Biophysics 2022Quote: ... The absence of RNases was confirmed using the RNase Alert Lab Kit (Ambion®).
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bioRxiv - Genomics 2022Quote: ... and supplemented with or without 40 U/ml RNAse inhibitor (RNAse OUT, Thermo Fisher) (Extended Data Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNase A-treated samples were pre-incubated with 20 ng RNase A (Invitrogen, AM2271) at 30°C for 10 minutes ...
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bioRxiv - Genomics 2022Quote: ... Further RNase activity was stopped by addition of SUPERaseIn RNase Inhibitor (Thermo Fisher Scientific). Next ...
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bioRxiv - Microbiology 2022Quote: ... or 300 units of RNase T1 (Fermentas) and 500 ng of RNase A (Ambion) or for 1 h at 4°C with 350 units of RNase I (Ambion) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNase I digestion was stopped by adding Superase-In RNase inhibitor (Thermo Fisher, AM2696) at 1 U/μl ...
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bioRxiv - Genomics 2023Quote: ... and 1 U/μL of RNase inhibitor (RiboLock RNase Inhibitor, EO0382, Thermo Fisher Scientific) in RPMI1640 ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNase I was then quenched with 1U/uL of Superase RNase Inhibitor (Ambion). RNA from ribosome protected fragments were recovered using a 1M sucrose cushion with ultracentrifugation (55,000 RPM ...
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bioRxiv - Molecular Biology 2023Quote: ... RNase inhibitor was omitted from reactions and 10 µg of RNase A (Thermo Fisher) was included ...
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bioRxiv - Biophysics 2022Quote: ... The water was purified through Barnstead GenPure water purification system (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... with Superase RNase inhibitor (Ambion) and random hexamers on 0.2–1 μg of RNA ...
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bioRxiv - Genomics 2022Quote: ... and RNAse H (Invitrogen 18021014) at 15 °C for 2.5 hours [8].
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bioRxiv - Biochemistry 2022Quote: ... and RNase inhibitor (Thermo Scientific) for 20 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and RNAse (RNaseOut, Thermofisher Scientific) inhibitors ...
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bioRxiv - Molecular Biology 2021Quote: ... and RNAse (RNaseOut, Thermofisher Scientific) inhibitors ...
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bioRxiv - Molecular Biology 2021Quote: ... treated with RNase A (ThermoFisher), phosphorylated with polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNase I (Thermo Fisher, AM2294) was added to lysate ...
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bioRxiv - Genomics 2020Quote: ... RiboLock RNase inhibitor (Thermo Scientific) was used instead of Murine RNase Inhibitor ...
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bioRxiv - Molecular Biology 2021Quote: ... and RNase Inhibitor (Fermentas/ThermoFisher). RNase A was added to remove RNA after reverse-transcription ...
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bioRxiv - Molecular Biology 2020Quote: ... RNAse Cocktail (Thermo Scientific, AM2286) was added to the mix in order to digest the RNA and the mix was incubated at 37°C for 10 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... and Rnase Out (Life Technologies) were added to the extracts ...
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bioRxiv - Molecular Biology 2019Quote: ... coli RNase H1 (Ambion #AM2293). Reactions were then stopped by addition of 10 μl of 2X Loading Buffer (95% formamide ...
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bioRxiv - Immunology 2019Quote: ... and RNase inhibitor (Fisher Scientific) at 72°C for 3 minutes on a thermal cycler to anneal the 3’ primer to polyadenylated mRNA ...
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bioRxiv - Plant Biology 2019Quote: ... and 50nl RNAse Inhibtor (Invitrogen) (with traces of Bromophenol Blue for optical verification ...
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bioRxiv - Systems Biology 2020Quote: ... and RNase inhibitor (Fisher Scientific) at 72°C for 3 minutes on a thermal cycler to anneal the 3’ primer to polyadenylated mRNA ...
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bioRxiv - Neuroscience 2020Quote: ... including RNase inhibitor (Applied Biosystems), with annealing for 10 minutes at 25°C and extending for 2 hours at 37°C and inactivation for 5 minutes at 85°C ...
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bioRxiv - Genetics 2021Quote: ... RNase A (Thermo Scientific 12091021) treatment was performed for 1 hour at 37°C.
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bioRxiv - Microbiology 2020Quote: ... and RNase A (Thermo Scientific) to remove all bacteria and free nucleic acid from the cell lysate ...
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bioRxiv - Cell Biology 2021Quote: ... and RNase H (Life Technologies). cDNA libraries were prepared for sequencing using the mRNA TruSeq protocol (Illumina).
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bioRxiv - Bioengineering 2022Quote: 1U RNase A (Thermo Fisher) was incubated with 1 μg dsRNA in 0.5 M NaCl (10 μl total volume ...