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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Approximately 3×106 cells were resuspended in 50 μl sterile HBSS (Gibco) and injected using a 28-gauge insulin needle via the retro-orbital plexus into CD45.1+ C57BL/6 lethally-irradiated recipients ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were split every 2 to 3 days using TrypLE Express (Gibco).
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bioRxiv - Bioengineering 2023Quote: ... Passage 3 cells were detached using 0.05% trypsin-0.53 mM EDTA (Gibco) and counted with Trypan blue (Thermo-Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... Calu-3 2B4 cells were grown in high glucose DMEM (Gibco #11965092) with 10% defined fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... 3) Dyes: NucBlue Live Cell Stain (2 drops/ml, R37605, Molecular Probes), phalloidin-568 (A12380 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged every 3 to 4 days using Accutase™ (Gibco) for up to 10 passages.
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bioRxiv - Immunology 2023Quote: ... Cells were washed 3 times with cold 1X PBS (Thermo Fisher Scientific) supplemented with 1% BSA (MACS Miltenyi) ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: Cells were passaged every 2-3 days by incubation with Accutase (Gibco) for 2-3 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Calu-3 2B4 cells were grown in high glucose DMEM (Gibco #11965092) with 10% defined fetal bovine serum ...
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bioRxiv - Immunology 2024Quote: ... cell suspension was mixed with 3 mL of ACK Lysing Buffer (Gibco). Remaining cells were stained and acquired using a FACSCalibur Cytek DxP 8 and analyzed with FlowJo ...
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bioRxiv - Evolutionary Biology 2022Quote: Females were mated as described in the “Sperm transfer and displacement” section and dissected in Grace’s supplemented insect medium (Thermo Fisher) under CO2 anesthesia 3-4 h AESM ...
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bioRxiv - Microbiology 2020Quote: ... Each insect was alive before it was immersed into RNAlater® Stabilization Solution (Cat. No. AM7021, Ambion, Austin, Texas, USA).
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bioRxiv - Developmental Biology 2020Quote: ... The tissue or organ was then preincubated for 30 min in 1 ml of Grace’s insect medium (Thermo Scientific, USA) at 30°C in the dark (Maestro et al. ...
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bioRxiv - Neuroscience 2021Quote: Adult female flies were fed for 2-4 days on wet yeast paste and ovaries hand dissected in Grace’s Insect Medium (Life Technologies). Ovaries were fixed in 4% formaldehyde (37% formaldehyde diluted in PBST (0.2% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... and PToV Hemagglutinin Esterase non-binding (F271A) (1176bp) fused to human IgG-Fc followed by a 6xHis tag in the Insect expression vector pFastBac1 (ThermoFisher) was kindly provided by Colin Parrish (PToV-HE_S46A-Fc_Hisx6/pFastBac1 ...
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bioRxiv - Biochemistry 2021Quote: ... of H5N1 virus were cloned into pFastBac vectors and were further packed into three insect viruses using a Bac-to-bac expression system (Invitrogen). The full-length FluPolH5N1 complex was expressed by insect cell line High Five after being infected by PA ...
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bioRxiv - Microbiology 2023Quote: ... they were carefully dissected using fine needles (insect pins, Shiga Konchu Fukyusha, Japan) attached to wooden chopsticks in ice-cold 1x Phosphate buffered saline (PBS) (Gibco 10 x PBS pH 7.4 ...
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bioRxiv - Evolutionary Biology 2024Quote: All Drosophila cells were maintained on either Shields and Sang M3 Insect Medium (MilliporeSigma S3652) or Schneider’s Insect Medium (MilliporeSigma S0146) supplemented with 10% v/v Fetal Bovine Serum (FBS, ThermoFisher A3160502). Cells were maintained in 4 mL of media in plug-seal T-25 flasks (Corning 430168 ...
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bioRxiv - Biochemistry 2022Quote: ... K562 cells were fractionated using the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher) according to maunfacturer’s instructions.
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... a single-cell suspension was created by treating the GBM cells for 3 minutes with accutase (StemPro Accutase Cell Dissociation Reagent, Gibco) at 37°C.
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bioRxiv - Neuroscience 2023Quote: ... Whole cell protein extraction was performed by lysing the cell pellet with Cell Lysis buffer (ThermoFisher, FNN0011) supplemented with protease inhibitor cocktail (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were stained with DAPI (Thermo) and FluoZin-3 AM (Invitrogen; to detect zinc-enriched β-cells), and resuspended in phenol red-free media ...
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bioRxiv - Developmental Biology 2021Quote: ... The number of viable cells was counted using the Countess 3 Automated Cell Counter (Thermo Fisher Scientific), according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged every 3-7 days as single cells using TrypLE™ Select CTS™ (Gibco). The ROCK inhibitor Y27632 (Fujifilm ...
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bioRxiv - Cell Biology 2020Quote: MEF R1441C cells were plated on 8-well dishes at 3×104 cells per each well (Nunc™ Lab-Tek™ II Chambered Coverglass from Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were allowed to reaggregate in U-bottom 96 well plates (3 × 103 cells per well) (Nunc). Differentiation medium was G-MEM-based (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... The Calu-3 cells or primary NHBE cells were seeded in a 96-well culture plate (Nunc, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the C4-2B or PC-3 cells were labeled by CellTrace™ Violet Cell Proliferation Kit (ThermoFisher) according to the manufacturer’s manual ...
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bioRxiv - Genetics 2023Quote: ... The cells were counted twice for each slide by Countess 3 Automated Cell Counter (Cat#AMQAX2000 Invitrogen), and the number of cells and percentage of live cells were recorded ...
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bioRxiv - Immunology 2021Quote: ... and proteins with a molecular weight above 100 kDa were separated on 3-8% Tris/Acetate gels (Life Technologies) in MOPS Buffer (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... Digitonin-solubilized mitochondria proteins (100 µg) were separated on pre-cast 3%-12% gradient blue native gels (Invitrogen #BN1001) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: 3 µg of anti-GFP (ab290) or IgG (sc2025) were mixed with 40 µl of Dynabeads Protein A (Invitrogen) and incubated at 4°C for 6 h with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant containing solubilized mitochondria was supplemented with Coomassie Blue G-250 dye to a final detergent:dye ratio of 16 g/g before running on a 3-12% NativePAGE Mini Protein Gel (ThermoFisher) according to manufacturer’s directions ...
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bioRxiv - Biochemistry 2021Quote: ... Samples and Blue Native Protein Ladder (Thermo LC0725) were loaded onto NativePAGE Bis-Tris Gels (3-12%, Invitrogen BN1001BOX) and gel electrophoresis was performed using anode NativePAGE Running Buffer (Invitrogen BN2001 ...
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bioRxiv - Microbiology 2021Quote: ... Protein melt curves were run in an Applied Biosystems Real-Time PCR System (QuantStudio 3, Thermo Fisher Scientific, USA). Data were collected and imported into Protein Thermal Shift Software (Version 1.3 ...
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bioRxiv - Immunology 2019Quote: ... while proteins above 200 kDa were blotted on a 3-8% Tris-Acetate gels in MOPS Buffer (Life Technologies). Proteins were separated at 200V for approximately one hour and transferred onto either PVDF or nitrocellulose membrane using the TransBlot Turbo Transfer (BioRad ...
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bioRxiv - Biophysics 2021Quote: ... and incubated (95 °C, 20 min) before SDS-PAGE using 3–8 % Tris-Acetate protein gels (Thermo Fisher Scientific). After transfer onto PVDF membranes (iBlot 2 Dry Blotting System ...
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bioRxiv - Biochemistry 2020Quote: ... 50-75 μg of proteins were loaded on Pre-cast Native PAGE 3-12% Bis-Tris gels (Invitrogen, #BN2011BX10) at 150 V and 4°C for 4 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... alongside aforementioned activating proteins and CellEvent™ Caspase-3/7 Green Detection Reagent (1:5000, ThermoFisher Scientific, Cat#C10423). Cells were imaged using the IncuCyte ZOOM System (Essen Bioscience ...
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bioRxiv - Neuroscience 2023Quote: ... Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH; Thermo Fisher Scientific 80196 for active-β-catenin ...
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bioRxiv - Biochemistry 2023Quote: ... 30 μl of the proteins at 3 μg/mL were plated onto 384-well Nunc Maxisorp plate (ThermoFisher, 464718) in 1x TBS and incubated 1h at 37°C followed by slap drying and blocking with 80 μL of Casein for 1 h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 30 μl of the proteins at 3 μg/mL were plated onto 384-well Nunc Maxisorp plate (ThermoFisher, 464718) in 1x TBS and incubated 1h at 37°C followed by slap drying and blocking with 80 μL of Casein for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 mg protein lysate used for streptavidin pulldown with 200 μL of streptavidin-coupled magnetic beads (ThermoFisher, 65002). The beads were washed by gently mixing with ...
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bioRxiv - Immunology 2021Quote: All recombinant proteins were produced using Expi293F cells (Life Technologies). Spike proteins for enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Immunology 2019Quote: ... Cell lysates were pre-cleared using protein A beads (Invitrogen). Pre-cleared lysates were immunoprecipitated using anti-lamin B1 antibody and these immunoprecipitated samples were incubated with protein A-sepharose beads (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: All proteins were expressed in BL21 DE3 star cells (Invitrogen), grown at 37°C (in LB media for unlabeled proteins and in M9 media for C-13 or N-15 labeled proteins ...
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bioRxiv - Immunology 2019Quote: ... Proteins were expressed in FreeStyle 293F cells (Thermo Fisher - R79007). About 25 mL Opti-MEM (Life Technologies – 31985-070 ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were placed in PFHMII protein free medium (GIBCO, ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were expressed in Expi293 suspension adapted cells (ThermoFisher Scientific). These cells were cultured in Expi293™ Expression Medium and transfection was achieved by mixing 2µg of cDNA with 6µg of polyethylenimine (PEI ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant protein was expressed using Expi293F™ Cells (ThermoFisher Scientific) following manufacturer’s protocol ...