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Citations for Thermo Fisher :
1201 - 1250 of 1270 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and two solid-phase extraction columns (150 µm × 3 cm) packed with MAbPac particles (C8 resin, 4 µm diameter, 300-Å pore size; Thermo Fisher Scientific) were used ...
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bioRxiv - Cell Biology 2023Quote: ... beads were bound to a dipping magnet (V&P Scientific #VP 407AM-N1) and washed 3 times in 150 µL ice-cold divalent-free D-PBS (Thermo Fisher Scientific #14190144) by serial transfers to 96-well plates ...
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bioRxiv - Neuroscience 2024Quote: ... in 5x volumes of ice-cold TBS buffer (50 mM Tris pH 7.4 [Thermo Scientific, J75825-A4], 150 mM NaCl [Fisher Scientific, BP358-10]) containing phosphatase and protease inhibitors (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized from 150 ng of total RNA extracted from heart tissues with use of a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Framingham, MA). RT-PCR was performed with QuantStudio (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... 1/200) and secondary antibodies were Donkey anti-rat Cy3 (Jackson 712-165-150 ; 1/500) Donkey anti-rat AlexaFluor488 (Invitrogen A21208 ; 1/500) and Donkey anti-mouse Cy3 (Jackson 715-165-150) ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was aspirated and the cell pellet was resuspended in 150 μL KCl buffer (for composition, see Ca2+ uptake protocol above) supplemented with 500 nM tetramethylrhodamine methyl ester (TMRM, Thermo Fisher Scientific, I34361) and 0.005% digitonin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The cultures were then collected and combined into a new sterile 150 mm x 15 mm petri dish (Fisher Scientific, Waltham, MA, USA). The plate was incubated in ambient light conditions (approximately 8 μmol photons m-2s-1 ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA expressing the Env ectodomain and furin were co-transfected at a 4:1 ratio (650 μg and 150 μg per transfection liter, respectively) and incubated with 293fectinTM transfection reagent (ThermoFisher Cat No. 12347019) in Opti-MEM I Reduced Serum Medium (ThermoFisher Cat No ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were loaded on an Acclaim PepMapTM RSLC C18 column (150 mm × 75 μm ID, 2 μm particles, 100 Å pore size) (Thermo Fisher Scientific), and eluted with a gradient of solvent B (19.92/80/0.08 v/v/v water/acetonitrile/formic acid ...
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bioRxiv - Microbiology 2024Quote: ... Around ten µg of peptides were applied onto a commercially available Acclaim PepMap 100 C18 column (2 µm particle size, 1 mm ID × 150 mm, 100 Å pore size; Thermo Fisher Scientific) and separated using a stepped gradient with acetonitrile concentration ranging from 3% to 24% to 31% solvent B (0.1% FA ...
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bioRxiv - Biophysics 2024Quote: ... Each cell was imaged using the focused ion beam and the location of the final 150 nm lamella was marked in AutoTEM (ThermoFisher Scientific, version 2.4.1). The lamellae were generated using a step-wise process ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or 150 heads or tails 3 hours post amputation (hpa) were transferred to a 2 mL tissue grinder (Fisher Scientific, 50-212-708) and washed with artificial seawater ...
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bioRxiv - Bioengineering 2024Quote: ... The muscle tissue cultures were then stained with 150 uL 1:100 Myosin4 antibody conjugated to eFluor™ 660 (Invitrogen, 50-6503-82) for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were eluted onto the separation column (PepMap™ Neo, 75 µm x 150 mm, 2 µm C18 particle size, Thermo Fisher Scientific) prior to elution directly to the mass spectrometer ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by primary antibody staining overnight at 4°C (Tuj1 (1:1000, made on site) and Sox10 (1:150, ThermoFisher, catalog PA5-37890). The next day ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in primary antibodies in 3% serum in PBS-T for up to 48 h at 4°C: anti-phospho-tau (ser202, thr205) (clone AT8) (ThermoFisher, MN1020; 1:150), anti-GFAP (Millipore-Sigma ...
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bioRxiv - Microbiology 2021Quote: ... 105 rabbit Sf1 epithelial cells were plated into each well of a 6-well plate and incubated at 37°C overnight in a 5% CO2 atmosphere within a HeraCell 150 incubator (Thermo Fisher Scientific, Waltham, MA). At the same time ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were separated on a 100 μm inner diameter microcapillary column packed with ∼40 cm of Accucore150 resin (2.6 μm, 150 Å, ThermoFisher Scientific, San Jose, CA). For each analysis ...
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bioRxiv - Biochemistry 2020Quote: ... with a flow rate of 5 μ l/min and separated on an EASY-Spray HPLC Column (75 μ m × 150 mm, Thermo Fisher Scientific). HPLC gradient was conducted using 5-55% of the mobile phase (75% acetonitrile and 0.1% formic acid ...
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bioRxiv - Biophysics 2021Quote: ... Cells were transfected 16–24 h prior to the experiment using between 50 ng and 150 ng plasmid per dish with Turbofect (HEK) or Lipofectamin3000 (A549) according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ... (primary Rabbit anti-mouse alpha-SMA antibody; Cat. No. ab5694; Abcam; 1:50 dilution; secondary Goat anti-rabbit antibody; Cat. No. A11008; Life Technologies; 1:150 dilution). Fluorescent images were acquired using a Leica SP8 confocal microscope ...
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bioRxiv - Plant Biology 2021Quote: Purifications were performed using a Waters 2795 Separations Module (Waters Corporation) and an Acclaim 120 C18 HPLC column (4.6 mm × 150 mm, 5 µm; Thermo Fisher Scientific, Waltham, MA, USA) with a column oven temperature of 30°C and flow rate of 1 mL/min ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were separated on a 100 μm inner diameter microcapillary column packed in house with ~35 cm of Accucore150 resin (2.6 μm, 150 Å, ThermoFisher Scientific, San Jose, CA) with a gradient consisting of 5%–21% (ACN ...
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bioRxiv - Immunology 2022Quote: ... chopped lung tissue was incubated at 37°C for 90 minutes (mins) in RPMI with 5% fetal bovine serum (FBS) and 150 U/ml collagenase (Life Technologies, Rockville, MD, USA). Nonadherent cells were enriched on Percoll density gradients (44/67% ...
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bioRxiv - Immunology 2020Quote: Dissected whole retina and lung tissue (positive controls) homogenates were prepared by the addition of 100 to 150 μL of lysis buffer (Invitrogen, Grand Island, NY, USA) with protease inhibitor cocktail (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... the medium was replaced with 1.75 ml fresh proliferation medium and the transfection mixture was prepared: A solution 150 µl Optimem (Gibco, Thermo Fisher Scientific. Ref: 31985-070) medium with 9 µl of lipofectamine (lipofectamine RNAiMAX Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... HUVEC at passage 3 were transfected twice at 24 h-intervals with 30 nM siRNA and Lipofectamine RNAi max (Life Technologies, ref. 13778-150) according to the manufacturer’s instructions in 37°C in a regular 5% CO2 in a humidified chamber ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were collected and lysed to extract total protein and RNA using CHAPS lysis buffer (30 mM Tris-Cl pH 7.5, 150 mM NaCl, 1% CHAPS) containing 100 units/mL RNaseOUT™ (Invitrogen; Life Technologies, Burlington, ON) on ice (20 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Fam/Cas dye (1:150) was added and incubated for 1 hour and washed with apoptosis wash buffer (Life Technologies V35118; diluted 1:10). Hoechst 1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... at a flow rate of 200 μL/min on an Accucore™-150-Amide-HILIC column 2.6 μm (100 × 2.1 mm) (Thermo Fisher Scientific, Cat 16726-102130) at 40 °C at a gradient from 45% to 90% A in 15 minutes followed by a 10-minute isocratic step ...
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bioRxiv - Molecular Biology 2022Quote: ... After nanoflow chromatography and electrospray ionization using an Acclaim PepMap 100 C18 HPLC column (100 Å, 2μm, 50 μm id × 150 mm, Thermo Fisher Scientific Inc., USA), peptide mixtures were injected onto the column at a flow rate of 300 nL/min using linear gradients from 1 to 100% v/v aqueous acetonitrile in 0.1% v/v formic acid over 60 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with 150 μL 1% BSA/PBS and then incubated with 50 μL Goat anti-Human IgG Fc secondary antibody PE (ThermoFisher, cat #12-4998-82) at a final concentration of 2.5 μg/mL in 1% BSA/PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 L of untreated sewage was collected and processed (150 mL) using ammonium sulphate precipitation and nucleic acid extraction using a Kingfisher Flex™ (Thermo Scientific™, UK) and NucliSENS® (BioMérieux ...
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bioRxiv - Immunology 2023Quote: ... chopped lung tissue was incubated at 37°C for 90 minutes (mins) in RPMI with 5% fetal bovine serum (FBS) and 150 U/ml collagenase (Life Technologies, Rockville, MD, USA). Nonadherent cells were enriched on Percoll density gradients (44/67% ...
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bioRxiv - Cancer Biology 2023Quote: ... The 8-well chamber walls were placed around the gels and 150-200 µl of hydroxyethyl agarose processing gel (Thermo Fisher, HG 4000-012) was poured into the well ...
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bioRxiv - Molecular Biology 2023Quote: ... siRNA transfections for cells at 10–40% confluency were carried out by forward (for Figure 4C, S1D, and S4B) or reverse (for other figures) transfection using Lipofectamine™ RNAiMAX (Invitrogen, Cat#13778-150) at a final concentration of 5–30 nM for 48 h ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 μL of cDNA were used in a reaction mixture with 150 nM of each primer and 250 nM of the probe and 1X TaqMan Fast Advanced Master Mix (Applied Biosystems, Foster City, CA). Reactions were run in a ViiA 7 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were separated on a 100 μm inner diameter microcapillary column packed with ~35 cm of Accucore150 resin (2.6 μm, 150 Å, ThermoFisher Scientific, San Jose, CA) with a gradient consisting of 5%–21% (ACN ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were mounted on a C18 separating column (2 µm particle size, 100 Å pore size, length 150 mm, inner diameter 50 µm, Thermo Fisher Scientific, 164711). An acetonitrile gradient (0–80% ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of each peptide from an individual sample was loaded onto a trap column and separated on a reverse-phase C18 column (50 μm×150 mm, 2 μm) using the EASY-nLC1200 HPLC system (Thermo Fisher Scientific, Waltham, MA). The elution for the analytical column lasted 120 min with a gradient of 5%–28% buffer B (0.1% formic acid in 80% acetonitrile ...
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bioRxiv - Biochemistry 2023Quote: ... and washed for 3 min at 30 μL/min before peptides were eluted onto a C18 Acclaim™ PepMap™ column (100 Å, 5 μm × 0.75 mm × 150 mm, Thermo Fisher Scientific) at a flow rate of 0.3 μL/min ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were mounted onto the formvar-coated 150-mesh copper grids for photography by a Tecnai G2 Spirit TEM (Thermo Fisher Scientific, MA, USA). Synaptic substructures were observed at the magnification power of 26,400 using TEM.
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of each peptide from an individual sample was loaded onto a trap column and separated on a reverse-phase C18 column (50 μm×150 mm, 2 μm) using the EASY-nLC1200 HPLC system (Thermo Fisher Scientific, Waltham, MA). The elution for the analytical column lasted 90 min with a gradient of 5%-28% buffer B (0.1% formic acid in 80% acetonitrile ...
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bioRxiv - Molecular Biology 2023Quote: ... and then separated on a C18-reverse phase analytical column (Double nanoViper™ PepMap™ Neo, 75 μm × 150 mm, particle size 2 μM, Thermo Fisher Scientific). The separation was achieved using a two-step gradient with mobile phases A (0.01% FA in H2O ...
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bioRxiv - Bioengineering 2023Quote: ... HUVECs at passage 3 were transfected twice at 24 h-intervals with 30 nM siRNA and Lipofectamine RNAi max (Life Technologies, ref. 13778-150) according to the manufacturer’s instructions in OptiMEM at 37°C in a regular 5% CO2 in a humidified chamber ...
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bioRxiv - Microbiology 2023Quote: ... was incubated with cross-linking buffer (20 mM sodium phosphate [pH 8], 150 mM NaCl) supplemented with 1.2 mM bis(sulfosuccinimidyl)suberate (BS3, Thermo Fisher Scientific catalog number A39266) for 30 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated on a 100 μm inner diameter microcapillary column packed in house with ∼35 cm of Accucore150 resin (2.6 μm, 150 Å, ThermoFisher Scientific, San Jose, CA) with a gradient consisting of 5%–16% (0-78 min) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of each peptide from an individual sample was loaded onto a trap column and separated on a reverse-phase C18 column (50 μm×150 mm, 2 μm) using the EASY-nLC1200 HPLC system (Thermo Fisher Scientific, Waltham, MA). The elution for the analytical column lasted 90 min with a gradient of 5%-28% buffer B (0.1% formic acid in 80% acetonitrile ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were plated at 20% confluence in complete DMEM supplemented with 150 μg/mL hygromycin B (Invitrogen, Thermo Fisher Scientific, Carlsbad CA, US). During selection ...
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bioRxiv - Synthetic Biology 2024Quote: MBP-PdhR-FUSN or PdhR proteins were thawed and desalted into the buffer used for phase separation experiments (PSB: 10 mM Tris, 150 mM NaCl, 30 mM MgCl2, pH 7.9) using Zeba spin desalting columns (Thermo Fisher Scientific, cat. no. 89882). MgCl2 was always added to the buffer directly before each experiment ...