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Citations for Thermo Fisher :
1001 - 1050 of 1270 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... was washed with buffer A (0.1 M sodium phosphate, 150 mM NaCl, 0.01% Tween20, pH 7.4) using DynaMag™-2 Magnet (Thermo Fisher Scientific, 12321D). After the wash ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The gel was run for approximately two hours at 150 V with the 1X NativePAGE Dark Blue Cathode Buffer being replaced with 1X NativePAGE Light Blue Cathode Buffer (Thermo Fisher) after the dye front had migrated one third of the way through the gel ...
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bioRxiv - Cell Biology 2023Quote: ... First Link) in TBS (20 mM Tris–Cl, pH 7.6, and 150 mM NaCl) supplemented with Tween-20 (10485733; Thermo Fisher Scientific), and probed with primary antibodies overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were checked on an Agilent Bioanalyser high sensitivity DNA chip (size range 150-2000bp) and quantified by Qubit dsDNA HS (Molecular Probes). Libraries were sequenced on Illumina® NextSeq 500 using 150bp paired end kits as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt, Germany; SOX9, 1:1000, #AB5535 EMD Millipore, Burlington, MA, USA; SOX2, 1:150, #14981182 Invitrogen), washed in washing buffer the next day ...
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bioRxiv - Plant Biology 2023Quote: ... blocked for 30 minutes in 3% BSA in 1x PBST and incubated with 150 nM Phalloidin-AF647 (#A22287, Thermo Fisher Scientific) in 3% BSA / PBST for 30 minutes in a humid chamber ...
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bioRxiv - Systems Biology 2023Quote: HEK293 200 µg peptides were separated by HpH reversed-phase chromatography using a reversed-phase Acquity CSH C18 1.7 μm × 1 mm × 150 mm column (Waters) on an UltiMate 3000 high-performance liquid chromatography (HPLC) system (Thermo Fisher Scientific) with the Chromeleon software ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... length 150 mm) using a 40 min linear gradient (300 nL/min) coupled to a Q Exactive Orbitrap mass spectrometer (Thermo Fisher) operated in data-dependent (phospho interactomics ...
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bioRxiv - Microbiology 2023Quote: ... MS data were obtained by nanocapillary reversed-phase LC-MS/MS using a C18 column (0.1 × 150 mm) on a nanoLC system (Advance/Michrom BioResources) coupled to an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific). The mobile phase consisted of water containing 0.1% formic acid (solvent A ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... 4 mm leaf discs of four weeks old Arabidopsis WT and mutant plants were incubated overnight (adaxial side up) in 150 µL of sterile water in 96-well plates (Thermo Fisher). The next day ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA (150 ng) was used to generate amplified sense strand cDNA targets using GeneChip WT Plus Reagent Kit (ThermoFisher Scientific) followed by fragmentation and labeling ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were plated at 8×105 per well in six-well plates and transfected with gRNA complex (crRNA:tracrRNA 10nM) in antibiotic-free medium using Lipofectamine RNA iMAX Reagent (Invitrogen #13778-150), according to the instructions of the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... Chromatographic separation was carried out using a Hypercarb porous graphitic carbon (PGC) column (5 µm, 150 mm x 1 mm, Thermo Scientific). A 40-minute binary gradient using 3% ACN in water containing 0.1% formic acid (Solvent A ...
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bioRxiv - Genomics 2023Quote: Cell lysates were prepared in chilled RIPA buffer (25 mM Tris-HCL pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Scientific, Rockford, IL) containing protease inhibitor (complete Mini ...
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bioRxiv - Molecular Biology 2023Quote: ... 37.5 µg affinity purified anti-CDCA7 or anti-HELLS antibodies was coupled to 150 µl Protein A Dynabeads (Thermo Fisher Scientific) and used to deplete 100 µl extract at 4 °C for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... Digested ribonucleosides were separated on Hypersil GOLD aQ reverse-phase column (3 µm particle size; 150 x 2.1 mm, Thermo Fisher Scientific) and detected using Agilent 6490 triple quadrupole LC/MS mass spectrometer in positive-ion mode ...
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bioRxiv - Molecular Biology 2023Quote: ... were injected onto the Acclaim PepMap 100 C18 column (3 µm x 0.075 mm x 150 mm) for the analysis on a Q-Exactive Plus instrument (Thermo Fisher Scientific) attached to an UltiMate 3000 UHPLC (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: The Holomonitor M4 digital holographic microscope was purchased from Phase Holographic Imaging (PHI, Sweden) and placed directly within a Heracell 150 CO2 incubator (Thermo Scientific). For digital holographic imaging experiments ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cells were cultivated with LB medium (150 rpm, 37°C) in AbgeneTM 96-well 2.2 ml polypropylene deep-well plates (Thermo Scientific, #AB0661) with breathable sealing film (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were collected and lysed in NP40 buffer (150 mM NaCl, 1% NP-40, 50 mM Tris-HCl, pH 8.0) with HALT protease inhibitor (Thermo Fisher Scientific). After 10 min incubation in Laemmli Sample Buffer in 95°C ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with siRNAs or microRNA mimics at 30 nM or as otherwise indicated using Lipofectamine RNAiMAX (Invitrogen 13778-150) and Opti-MEM (Gibco 31985070 ...
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bioRxiv - Neuroscience 2024Quote: Tissue lysis and protein extraction was completed by adding 25 µL of 5x RIPA buffer (50 mM Tris, pH 8.0 [Thermo Scientific, J75825-A4], 150 mM NaCl [Fisher Scientific, BP358- 10] ...
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bioRxiv - Immunology 2024Quote: ... and run at 150 V for 1 hr with NuPAGE MOPS sodium dodecyl sulfate (SDS Running Buffer, 20X, Thermo Scientific Inc). Following electrophoresis ...
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bioRxiv - Biophysics 2024Quote: ... The protein fraction was isolated by lysing the cells using Lysis buffer + HALT (25 mM Tris-HCL, 150 mM NaCl, 1 mM EDTA, 1% Triton, HALT [Thermofisher, MA]) and in accordance with the western blot protocol used by Zagotta and coworkers (Zagotta et al. ...
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bioRxiv - Immunology 2024Quote: ... Cells (2 × 105) were washed twice with wash buffer (20 mM HEPES (H3375, Sigma-Aldrich], 150 mM NaCl (cat. AM9760G, Invitrogen), 0.5 mM spermidine (cat ...
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bioRxiv - Cell Biology 2024Quote: ... PASMCs were transfected with 5 pmol of each siRNA using Lipofectamine RNAiMAX transfection reagent (cat # 13778-150, Life Technologies; Carlsbad, CA). After overnight transfection ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µL of sample was injected and subjected to reverse phase chromatography with an Accucore C30 150 x 2.1 mm column (ref 27826-152130, Thermo Fisher Scientific), operated at 45 °C on an Agilent 1290 HPLC system ...
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bioRxiv - Physiology 2024Quote: ... 20 ug peptides were loaded onto a Kinetex 2.6u EVO C18 100 Å 150 x 0.3 mm column via an EASY-nLC 1200 HPLC (Thermo Fisher Scientific) in buffer AF (10 mM TEAB) ...
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bioRxiv - Immunology 2024Quote: ... stained with Alexa Fluor 488 conjugated EF5 antibody (clone ELK3-51, 150 μg/ml) and mounted onto histological slides with Prolong Diamond Antifade mounting medium (#P36965, Thermo Fisher).
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from 100-150 mg of flash-frozen rat somatosensory cortex tissue using TRIzol reagent (ThermoFisher Scientific #15596026) as previously described (Rio et al. ...
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bioRxiv - Immunology 2024Quote: ... or NP-40 lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 1% NP-40, pH 7.4) containing protease inhibitor (#78429; Thermo Fisher Scientific) for 30 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... overnight cultures were diluted to a final absorbance of 0.1 (OD600) within 150 µL BHIS in anaerobic conditions and a plastic adhesive film (adhesive sealing sheet; Thermo Scientific, AB0558) was used to seal the plate ...
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bioRxiv - Developmental Biology 2024Quote: ... with the exception that tissues were extracted in a non-denaturing lysate extraction buffer (50 mM Tris-base, 150 mM NaCl, 20 mM NaF) supplemented with Pierce Protease Inhibitor Cocktail (ThermoFisher Scientific) containing Aprotinin ...
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bioRxiv - Microbiology 2024Quote: ... the flow was directed to an Acclaim PepMap RSLC C18 analytical column (75 µm × 150 mm, C18 reversed phase, particle size: 2 µm; Thermo Scientific). The mobile phase A was 0.1% v/v formic acid in water and the mobile phase B was 0.1% v/v formic acid in acetonitrile ...
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bioRxiv - Molecular Biology 2024Quote: Enriched cysteines were loaded onto a 100-µm capillary column packed with 25 cm of Accucore 150 resin (2.6 μm, 150Å; Thermo Fisher Scientific) and separated using a 180-minute method on a NanoLC-1200 UPLC system ...
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bioRxiv - Immunology 2024Quote: On day 6 post-infection mice were intravenously injected with 150 µL/mouse of 2% Evans blue (Fisher Scientific, Waltham, WA). After two hours ...
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bioRxiv - Physiology 2024Quote: ... 20 ug peptides were loaded onto a Kinetex 2.6u EVO C18 100 Å 150 x 0.3 mm column via an EASY-nLC 1200 HPLC (Thermo Fisher Scientific) in buffer AF (10 mM TEAB) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown in 150 mm dishes to ∼90% confluence then washed with sterile PBS and detached (Gibco TrypLE Express #LTS12604021). Cells used for all experiments were ≤9 passages from stock and tested negative for mycoplasma contamination (Agilent MycoSensor QPCR Assay Kit ...
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bioRxiv - Biophysics 2024Quote: Purified wild-type or mutant S-OPA1 proteins were buffer exchanged into the liposome buffer (20 mM HEPES-NaOH, pH 7.5 and 150 mM NaCl) using micro-spin desalting columns (Thermo Scientific Zeba). Equal volumes (25 μl ...
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bioRxiv - Neuroscience 2024Quote: ... The reference and 37°C samples were adjusted to 150 ng/µL with 0.1% FA in 4% ACN supplemented with 0.015% n-dodecyl-β-maltoside (Thermo Fisher Scientific) and remaining temperature sample volumes adjusted as described above.
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bioRxiv - Biochemistry 2024Quote: ... Protein was flash-frozen in pH 7.5 buffer or buffer exchanged into “lysosomal pH buffer” (25 mM sodium acetate pH 4.5, 150 mM NaCl) using a Zeba 7k MWCO spin column (Thermo Scientific, PI89882) according to manufacturer instructions and diluted to a final concentration of 1 mg/mL.
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed in 0.01 M PBS for 3 times (5 min each time) and then incubated with the secondary antibody (goat anti-rabbit Alexa Fluor 568, 1:150 diluted in TBS ThermoFisher, #A11011) for 2 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The sample injection volume was 45 µL and the separation was performed with a C18 column (150 mm × 3 mm Accupore PFP, ThermoFisher Scientific). The mobile phase consisted of a mixture of two solvents ...
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bioRxiv - Pathology 2024Quote: ... and 3 µL were loaded on an Accucore reverse phase C18 column (2.1 × 150 mm, 2.6 μm, 80 Å; Thermo Fisher Scientific) installed on a Vanquish Flex UHPLC (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... The imidazole was removed by dialyzing the protein solution against 20 mM Tris pH 7.5 and 150 mM NaCl to a million-fold dilution using 3.5 KDa cutoff dialysis tubing (Snakeskin, Cat# 88244, Thermo Fisher Scientific). The purified protein was flash frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Bioengineering 2024Quote: ... Mouse syngeneic Py8119 breast cancer cells suspended at 1–2 million/150 μL of 33% (v/v) Matrigel (Thermo Fisher Scientific) were orthotopically injected into 6-8-week-old female C57BL mice (Jackson Laboratories) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested by centrifugation and lysed by sonication in binding buffer (25 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM DTT, protease inhibitor cocktail (Thermo Scientific #A32965). The cell suspension was cleared by centrifugation at 40,000 rpm for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... and the analytes were separated on an EASY-Spray PepMap Neo Column (3 μm, 0.075 mm × 150 mm, Thermo Scientific, CA). LC separation was performed with a binary gradient using solvent A with 0.1% (v/v ...
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bioRxiv - Biophysics 2024Quote: ... The fixed cells were washed three times with PBS and then incubated with primary antibodies, dilution of 1:150 for anti-αSyn (Cloud Clone, #PAB222Hu01) and 1:500 anti-H3 (Invitrogen, # AHO1432) or 1:1000 anti-H2b (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 minutes in a dark humidity chamber followed by incubation with primary antibodies ZO-1 (1:150, #40-2200, Invitrogen, Germany) and E-cadherin (1:400 ...