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Citations for Thermo Fisher :
751 - 800 of 1270 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Lipids were separated using a C30 Accucore LC column (150 mm x 2.1 mm, 2.6 µm particle size) and a Transcend UHPLC pump (Thermo Fisher Scientific). Liquid chromatography was performed with solvents ...
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bioRxiv - Biophysics 2022Quote: ... A mastermix of reverse transcription reaction reagents was prepared and aliquoted in each reaction to reach a total of 20 μL yielding a final concentration of 150 SSIV (ThermoFisher) units ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were denatured at 75 °C for 20 min and run at 150 V for 1.5 h on 4-12% NuPAGE Bis-Tris gels in NuPAGE MOPS running buffer (Thermo Fisher). Proteins were transferred to polyvinylidene difluoride membrane using NuPAGE transfer buffer (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 150 nM v3’ template RNA (FluPolA: 5’-AGUUUGCCUGCUUCUGCU-3’, FluPolB: 5’-UAUACCUCUGCUUCUGCU-3’) and 250 µM NTP mix (ThermoFisher). 50 µM CTD peptides were added at concentrations corresponding to at least a 10-fold excess over the KD of the lowest measured affinity for a two-repeat peptide ...
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bioRxiv - Bioengineering 2022Quote: ... Media from the top well was replaced with 100 µL of the fluorescent small molecule solution (200 µg/ml 10 kDa Dextran conjugated to AF488, 1 mg/ml 10 kDa Dextran conjugated to FITC, or 150 µg/ml lucifer yellow 457 Da, all Invitrogen), and fluorescent time-lapse imaging began as immediately as possible (10 kDa Dextran conjugated to AF488 ...
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bioRxiv - Biochemistry 2022Quote: ... Chromatographic separation was carried out on an Aquasil C18 analytical column (4.6 mm x 150 mm, 5 mm; Thermo Scientific). MNA was eluted with the mobile phase consisting of acetonitrile (A ...
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bioRxiv - Biochemistry 2022Quote: Samples were analysed by IP-RP-HPLC on a U3000 HPLC system using a DNAPac RP (150 mm x 2.1 mm I.D. Thermo Fisher Scientific). Chromatograms were generated using UV detection at a wavelength of 260 nm ...
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bioRxiv - Developmental Biology 2022Quote: ... used at 1:5,000 in MAB buffer (100 mM Maleic acid, 150 mM NaCl, and pH 7.5) + 20% heat-inactivated lamb serum (Gibco 16070096) + 2% blocking reagent (Sigma 11096176001) ...
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bioRxiv - Microbiology 2021Quote: ... The sample with the largest DNA concentration (15ng/μl) measured using the Qubit fluorometer (ThermoFisher Scientific, 150 Waltham, MA, USA) was selected for sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... coli digests and Promega yeast protein digest were each fractionated by offline reversed-phase LC (Waters Acquity UPLC Peptide BEH300 C18 1.7 µm, 1 mm i.d. x 150 mm column employing an Ultimate 3000 HPLC, Thermo Fisher Scientific) operating with column compartment temperature of 60 °C and flow rate of 100 µL/min ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, supplemented with Halt protease inhibitor cocktail [Thermo Fisher Scientific]) to obtain whole-cell lysates or lysed using the NE-PER nuclear and cytoplasmic extraction kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... subsequently suspended in phage buffer (10 mM Tris-HCl, Sigma,10 mM MgSO4, 150 mM NaCl, pH 7.5, Fisher Scientific), and stored at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... DMEM 10 % FBS for at least 48 h then 150 000 cells were reverse transfected with 500 ng of GFP using lipofectamine 2000 (Invitrogen) in 24 well plate.
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bioRxiv - Microbiology 2021Quote: ELISA assays were performed in 96-microwell plates coated with 150 ng/well of SARS-CoV-2 Spike (aa 16-1213) His-tagged recombinant protein (ThermoFisher) and 1010 phage or AAVP particles/50 μL of PBS O.N ...
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bioRxiv - Developmental Biology 2021Quote: Cells and organoids were lysed in modified radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 7.4, 0.2% SDS (Invitrogen, cat# 15553), and 0.1% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: Lyophilized full-length WT aSyn protein was dissolved for in vitro studies in Tris buffer (50mM Tris, 150 mM NaCl, pH 7.5, TBS) or phosphate-buffered saline (PBS, Invitrogen, Switzerland) for the cellular studies ...
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bioRxiv - Systems Biology 2022Quote: ... equipped with an Acclaim PepMap RSLC RP C18 separation column (50 μm x 150 mm, 2μm) (Thermo Scientific, Waltham, USA), and an QE plus Orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were resuspended in 1 ml IP lysis buffer [25 mM Tris-HCl pH 7.5 / 150 mM NaCl / 1% NP-40 / 1 mM EDTA / 5% glycerol / Halt protease inhibitor cocktail (Thermo scientific) / Halt phosphatase inhibitor cocktail (Thermo scientific)] and incubated on ice for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... 8% Tris-glycine gels at 150 volts for 2.25 hr or pre-poured NuPAGE 3-8% Tris-Acetate gel (Invitrogen; EA03752) for 2.15 hr (Figure 1C ...
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bioRxiv - Cell Biology 2020Quote: ... The 100 μm capillary column was packed with 30 cm of Accucore 150 resin (2.6 μm, 150Å; Thermo Fisher Scientific). Mobile phases were 5% ACN ...
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bioRxiv - Pathology 2020Quote: ... cDNA was reverse transcribed from 100-150 ng of total RNA using the SuperScript VILO Master Mix (Thermo Fisher Scientific) with ezDNAse treatment and used (1-5 ng/reaction ...
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bioRxiv - Immunology 2020Quote: Stimulated T cells were transfected with 150 pmol siRNA at 1600 V for 10 ms with three pulses using the NEON transfection system (ThermoFisher). 24 hours later ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were washed with PBS and incubated with Alexa Fluor 488 donkey anti-mouse antibody (Invitrogen, A21202, 1:150) for 1 h at 25°C ...
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bioRxiv - Microbiology 2021Quote: ... One brain hemisphere or a whole brain from an infected mouse was homogenized with a Wheaton Potter-Elvehjem Tissue Grinder with a 100-150 µm clearance (ThermoFisher) in PBS and an aliquot of the homogenate was viewed under a epifluorescence microscope (Nikon ...
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bioRxiv - Molecular Biology 2021Quote: ... all proteolytic digests were acidified by addition of 10% formic acid to pH 2 and directly analyzed by LC-ESI-MS(/MS) using an a capillary BioBasic C18 reversed-phase column (BioBasic-18, 150 x 0.32 mm, 5 μm, Thermo Scientific), installed in an Ultimate U3000 HPLC system (Dionex) ...
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bioRxiv - Cell Biology 2020Quote: ... The beads were washed three times with the corresponding lysis buffer (+ 150 mM NaCl in case of eGFP-Cep152) and eluted by cooking in NuPAGE LDS Sample Buffer (Invitrogen) with 5 mM DTT.
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bioRxiv - Microbiology 2020Quote: ... mice were intravenously injected with 150 µl of 10 kDa dextran conjugated with Alexa Fluor 680 (D34680; Thermo Fisher Scientific) at a concentration of 1 mg/ml in sterile PBS29 ...
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bioRxiv - Pathology 2021Quote: ... cells were lysed with lysis buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 1% NP40) supplemented with protease inhibitor cocktail (Thermo Scientific). Cell lysates were separated by SDS-PAGE and transferred onto a PVDF membrane by a semi-dry transfer (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... cDNA was made from 150 or 200 ng of total RNA using oligo (dT) primer with SuperScript IV reverse transcriptase (Invitrogen) for qPCR or RT-PCR analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Fisher) containing 1x protease inhibitor cocktail III (Calbiochem ...
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bioRxiv - Biochemistry 2021Quote: ... lysis buffer (1% Triton X-100, 150 mM NaCl, 50 mM TEA pH 7.4, 100xEDTA-free Halt Protease Inhibitor [Life Technologies]). The lysates were transferred to 1.5-ml Eppendorf tubes (Corning) ...
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bioRxiv - Systems Biology 2021Quote: ... Native protein complexes from platelets were obtained by incubating the washed pallet with Pierce IP lysis buffer (25 mM Tris•HCl pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol) (Thermo Scientific) containing protease and phosphatase inhibitors at 4 °C for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: 1 µg of peptide was injected into a pre-packed 0.075 mm x 150 mm Acclaim Pepmap C18 LC column (2 µm pore size, Thermo Fisher) attached to a Q Exactive Plus (Thermo Fisher ...
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bioRxiv - Biophysics 2021Quote: ... The cell suspension was mixed with 150 μL FACSFlow (Fisherscientific: 12756528) and 0.5 μL of propidium iodide (∼15 mM, ThermoFisher: P3566). The propidium iodide was excited with a 488 nm argon ion laser equipped within the Becton-Dickinson flow cytometer (FAC-SCalibur) ...
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bioRxiv - Biophysics 2020Quote: ... Cells were washed and incubated with secondary Alexa Fluor®-labeled antibodies (Life Technologies; diluted 1:150 in 1% BSA) for 1 hour at room temperature.
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bioRxiv - Microbiology 2020Quote: Twitching assays were performed as described previously43,44 except that assays were performed in 150 mm by 15 mm petri polystyrene dishes (Fisher Scientific) with 30 µg/ml gentamicin for 18 h at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was then recovered and sonicated to a length between 150 and 200 bp and biotinylated DNA fragments were purified using streptavidin beads (MyOne C1, Invitrogen). The DNA was then end-repaired and ligated to hairpin adaptor BU2 and amplified by PCR ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots (150 μl) were read in 96 well plates with black opaque sides and clear bottoms (Thermo Scientific Nunc 165305). GFP and tdtomato fluorescence and optical density at 600 nm was read with a BioTek Synergy 2 plate reader using 485/20 excitation and 516/20 emission filters for GFP and 545/40 excitation and 590/20 emission and filters ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots (150 μl) were read in 96 well plates with black opaque sides and clear bottoms (Thermo Scientific Nunc 165305). GFP and tdtomato fluorescence and optical density at 600 nm was read with a BioTek Synergy 2 plate reader using 485/20 excitation and 516/20 emission filters for GFP and 545/40 excitation and 590/20 emission and filters ...
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bioRxiv - Immunology 2020Quote: ... Each mouse received 50 μg of antigen in 150 μL of PBS (NP40‑FICOLL) or PBS/Alum (77161, Thermo Fisher) adjuvant (2:1 ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotin was attached to cross-linked RNA duplexes by incubating with 150 µM Click-IT Biotin sDIBO Alkyne (Life technologies) under constant agitation at 37 °C for 1.5 hours ...
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bioRxiv - Immunology 2020Quote: ... The cells were co-transfected with 650 μg of SOSIP expressing plasmid DNA and 150 μg of furin expressing plasmid DNA complexed with 293Fectin (Invitrogen). The cell culture supernatant clarified on day 6 and subjected to positive PGT145 antibody affinity chromatography ...
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bioRxiv - Microbiology 2021Quote: ... 2×105 cells were resuspended in 150 μl of culture medium containing 15 μl of ultracentrifuged lentiviral vectors supplemented with 2mM HEPES (Gibco) and 4 μg/ml polybrene (Sigma) ...
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bioRxiv - Cancer Biology 2021Quote: ... 99.9% N2 and 0.1% O2 atmosphere using a BINDER C-150 incubator (BINDER GmbH) and complete RPMI 1640 and DMEM media without glucose (Gibco). In re-oxygenation experiments ...
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bioRxiv - Microbiology 2021Quote: ... BN-PAGE gels were run for 2 to 2.5 hours at 150 V using the NativePAGETM running buffer (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Immunoprecipitation was performed on 150 μg of chromatin quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) with 60 μl of Protein-A/G Dynabeads and 3.5 μl of anti-GFP (Thermo Fisher #A11122 ...
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bioRxiv - Cancer Biology 2023Quote: ... The analytical gradients were carried out using an Accucore 150-Amide-HILIC column (2.6 μm, 2.1 mm × 100 mm, Thermo Fisher Scientific) with solvent A (5 mM ammonium acetate in 5% acetonitrile ...
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bioRxiv - Plant Biology 2023Quote: ... Chromatographic separation was achieved on the Hypersil GOLD C18 Selectivity HPLC Columns (150 × 4.6 mm; 3 μm; Thermo Scientific™) with mobile phases consisting of water (A ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were placed in 24-well plates and incubated with freshly prepared 150 μg/mL D-Luciferin in DMEM/F12 medium (Gibco) for 2 minutes prior to imaging on the IVIS for 1 minute using FOV A ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with DMEM F:12 containing MitoTracker DeepRed FM at a concentration of 150 nM (Thermo Fisher, M22426) at 37°C for 45 minutes ...