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Citations for Thermo Fisher :
801 - 850 of 1270 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... we seeded cells at 10∼20% confluency in 150 mm tissue culture dishes (Fisher Scientific, Cat No: 12-565-100) containing 30ml of exosome-free medium ...
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bioRxiv - Biophysics 2023Quote: ... the surface was rinsed three times with DNA resuspension buffer (150 mM STE-buffer, BP2478-1, ThermoFisher scientific, Geel, Belgium) without letting the surface dry out ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining supernatant was incubated with 150 µl of KPBS prewashed anti-HA magnetic beads (Thermo Fisher Scientific cat# 88837) on a gentle rotator shaker for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... was diluted in assay buffer (2 μM in 10 mM HEPES, 150 mM NaCl) containing Sypro Orange (1 in 1000 dilution; Thermofisher). Compounds ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated on a 100 μm inner diameter microcapillary column packed with 35 cm of Accucore C18 resin (2.6 μm, 150 Å, ThermoFisher). For each analysis ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2000 cells in 150 µl of media were plated in each well of 96-well ultra-low attachment plates (174929, Thermofisher). Cells were centrifuged at 100g for 3 min and incubated at 37°C (in 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: Cells were washed twice with PBS and lysed with RIPA buffer (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Fisher; 89901) supplemented with 1 mM EDTA (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Metabolites were separated using a XSelect HSS C18 column (2.1 mm x 150 mm, 3.5 μm particle size) (Waters, Milford, MA) in an UltiMate 3000 quaternary UHPLC (Thermo Scientific) equipped with a refrigerated autosampler (5°C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was extracted via homogenization with a Dounce or plastic pestle homogenizer in 150-300 μl RIPA buffer containing 1× Halt Protease inhibitor cocktail (ThermoFisher) and 1x PhosSTOP (Roche or Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Embryoid bodies (EBs) were grown by seeding 9000 cells in 150 μL of DMEM/F12 medium (Life Technologies, 11330-032) supplemented with 10% knockout serum replacement (Life Technologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Peptides were separated on an in-house packed 100 μm inner diameter column packed with 35 cm of Accucore C18 resin (2.6 um, 150 Å, ThermoFisher), using a gradient consisting of 5–35% (ACN ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 min and washed extensively with DNA buffer (20 mM Tris pH 7.5, 150 mM NaCl, 0.25 mg/ml BSA (ThermoFisher Scientific; AM2616)) ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 µl of saturated cell culture (2 million cells/ml) was mounted on a frosted microscope slide (Fisher Scientific #1255015) via cytocentrifugation on a Shandon Cytospin 3 Cytocentrifuge (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatographic separation was performed on a Vanquish UHPLC system with a Cadenza CD-C18 3 µm packing column (Imtakt, 2.1 mm id x 150 mm) coupled to a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) via an Ion Max ion source with a HESI II probe (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... with a Cadenza CD-C18 3 µm packing column (Imtakt, 2.1 mm id x 150 mm) coupled to a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) via an Ion Max ion source with a HESI II probe (Thermo Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... Thin cuts (from 50 to 150 µm) were performed using a vibrating blade microtome (Microm HM 650V ®, Thermo Scientific). Sections were labelled using calcofluor white and propidium iodide as previously described (Musseau et al. ...
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bioRxiv - Genetics 2024Quote: ... tryptic peptides were then separated by reversed-phase XSelect CSH C18 2.5-μm resin (Waters) on an in-line 150 x 0.075–mm column using an UltiMate 3000 RSLCnano system (Thermo Fisher). Peptides were eluted using a 60-minute gradient of buffer A/buffer B from 98:2 to 65:35 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood as described for the PBMCs from the patients and cryopreserved at -150°C in fetal calf serum (FCS, Gibco) with 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... EBs were seeded at 100–150 EBs per T175 or 250–300 per T225 flask in factory medium consisting of OXM4 supplemented with Glutamax (Gibco), 50 µM β-mercaptoethanol (Gibco) ...
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bioRxiv - Immunology 2024Quote: Harvested supernatant was diluted 2× in Tris-Hcl 50 mM NaCl 150 mM CaCl2 2 mM pH 8.0 (binding buffer) and incubated with immobilized p-aminophenyl phosphoryl choline beads (ThermoFisher Scientific) at room temperature for one hour ...
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bioRxiv - Genomics 2024Quote: ... The RNA samples were adjusted to similar concentration and 150-250 ng of RNA was used in reverse transcription with Superscript III (ThermoFisher). Transcript levels were quantified on a StepOnePlus Real-Time PCR system using the SYBR green PCR mix (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: O/N cultures were diluted 10000-fold in BHI and 150 μl inoculated into a round bottom 96-well plate (Nunc) to initiate biofilm formation in microtiter plates ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was performed at 4°C for 30 min in dark blue cathode at 150 V (Thermo Fisher Scientific, BN2007). After 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... cells in suspension were held at 150 psi for 20 min on ice in Pierce Protease Inhibitor Cocktail (Thermo Scientific) in a 45 mL Cell Disruption Vessel (Parr Instrument ...
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bioRxiv - Cell Biology 2024Quote: ... The 100 µm capillary column was packed in-house with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). Data were acquired for 180 min per fraction ...
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bioRxiv - Cell Biology 2024Quote: ... A 100 µm capillary column was packed in-house with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). Data were acquired for 180 min per fraction ...
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bioRxiv - Developmental Biology 2023Quote: ... used at 1:5000 in MAB buffer (100 mM Maleic acid, 150 mM NaCl, and pH 7.5) + 20% heat-inactivated lamb serum (Gibco 16070096) + 2% blocking reagent (Sigma 11096176001) ...
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bioRxiv - Neuroscience 2024Quote: ... 5 μL of supernatant was injected onto a Microsorb-MV column (C18, 150 mm x 5.6 mm, 5 micron) using an Ultimate 3000 UHPLC system (Thermo Fisher). Separation was achieved with a 17-minute isocratic method at a flow rate of 0.6 mL/min ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were analyzed on an Accucore C30 LC column (150 mm × 2.1 mm 2.6 µm Solid Core; Thermo Fisher Scientific) connected to a Shimadzu Nexera HPLC system and a QTRAP 5500 LC-MS/MS (SCIEX ...
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bioRxiv - Neuroscience 2023Quote: ... 7 x 106 AAV-293 cells were seeded into 150 mm cell culture plates in DMEM (# 41965062, Thermo Fisher Scientific) containing 10% fetal bovine serum (#16000-044 ...
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bioRxiv - Plant Biology 2023Quote: ... Patches of cells were scraped off from individual plates using 10 μL inoculation loops and resuspended in 1 mL of Agrobacterium induction buffer (10 mM MES pH 5.6, 10 mM MgCl2 and 150 uM acetosyringone; Acros Organics) in individual 2 mL safe-lock tubes (Eppendorf) ...
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bioRxiv - Systems Biology 2024Quote: ... Peptide eluents were separated on a custom in-house packed Charged Surface Hybrid (CSH) column (1.7 um, 15 cm × 150 μM internal diameter) by a Dionex RSLCnano running capillary flow UPLC (ThermoFisher Scientific). Buffer A comprised water with 0.1% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... The 100 µm capillary column was packed in-house with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). The peptides were separated using a 180 min linear gradient from 5% to 32% buffer B (90% ACN + 0.1% formic acid ...
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bioRxiv - Biochemistry 2023Quote: ... The 100 µm capillary column was packed in-house with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). Data were acquired for 180 min per run ...
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bioRxiv - Systems Biology 2023Quote: ... then transfected with siRNAs of target genes or scramble controls for 3 days using Lipofectamine RNAiMAX (Invitrogen, cat# 13778-150). The culture medium was replaced with 150µl of differentiation medium DM-2 (Zenbio ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then incubated in primary antibody with 5% goat serum in PTX, mouse-nc82 (1:20) (Development Studies Hybridoma Bank, University of Iowa) and rabbit-antiGFP (1:150) (Invitrogen) in PTX for 48 hours in 4°C while rotating ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein was isolated from these brains using RIPA buffer (25 mM Tris HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Scientific #89900) supplemented with 1X Protease Inhibitor Cocktail (Roche # 5892791001 ...
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bioRxiv - Immunology 2022Quote: ... from healthy donors were isolated from whole blood by density centrifugation on Lymphoprep (Axis-Shield PoC) and cryopreserved at −150 °C in FCS (Gibco) + 10% DMSO.
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bioRxiv - Biochemistry 2022Quote: ... 9 × 106 HEK293T cells were seeded the day before transfection in 150 mm dishes in Dulbecco’s modified Eagle medium (DMEM, Gibco Life Technologies) with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: ... 150 nM αSO (concentration in α-syn monomer units) in PBS buffer was loaded on to 96-well plate (Nunc flat clear bottom ...
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bioRxiv - Biochemistry 2022Quote: ... reaction mixtures containing approximately 5 μM protein in DSF buffer (50 mM Na2HPO4, 150 mM NaCl, pH 7.4) and 1x Protein Thermal Shift Dye (Thermo Fisher) were heated from 20 °C to 99 °C ...
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bioRxiv - Immunology 2022Quote: HEK 293T were co-transfected with pmCherry/ pmCherry-SLFN11/ pmCherry-SLFN12 (12 μg) and HIV-1-pNL-E (12 μg) using Lipofectamine 2000 (48 μl) in a T-150 culture dish (ThermoFisher). In order to freeze elongation ribosomes ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were purified on the semipreparative LC using 200 µL injections at a flow rate of 1.5 mL/minute using a C18 semipreparative column at 30 °C (Acclaim C18 5 µm 120 Å, 4.6 x 150 mm, ThermoFisher). Solvents A and B were ...
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bioRxiv - Microbiology 2022Quote: ... TcdB protein was diluted to 0.05 µg/µL using phosphate buffer (100 mM KPO4, 150 mM NaCl, pH 7) containing 5X SYPRO Orange (Invitrogen S6650), and a serial dilution of test compound ...
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bioRxiv - Microbiology 2022Quote: ... Mix 1 was prepared by adding 30 pmol of siRNA to 150 µl non-supplemented Opti-MEM medium (Thermo Fisher) and then gently mixing ...
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bioRxiv - Microbiology 2022Quote: ... samples were washed with PBS and resuspended in 100 mM HEPES and 150 mM NaCl containing a 1:4000 dilution of Syto-BC (Invitrogen). Samples were run and acquired on a BD Aria II maintained in a laminar flow biosafety cabinet ...
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bioRxiv - Bioengineering 2023Quote: ... Reverse Phase separation was employed with an Accucore™ C30 column (2.1×150 mm, 2.6 μm particle size) on a Vanquish (ThermoFisher Scientific) chromatograph ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were analyzed on an Accucore C30 LC column (150 mm x 2.1 mm 2.6 μm Solid Core; Thermo Fisher Scientific) connected to a Shimadzu Nexera HPLC system and a QTRAP 5500 LC-MS/MS (SCIEX ...
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bioRxiv - Immunology 2022Quote: ... 5 μl of mRNA-1273 (200 ng/μl) were diluted in 150 μl of Opti MEM media (Thermo Fisher Scientific), at room temperature and after 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... Luciferase activity was measured at 48 h post-transfection in cells treated for 5 min with 150 μg/mL D-Luciferin potassium salt (ThermoFisher) using an IVIS Lumina III imaging system (Caliper Life Sciences) ...