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Citations for Thermo Fisher :
1101 - 1150 of 1270 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Lysis buffer (20 mM Tris pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1% Triton X-100, 1 mM DTT, 25 U/ml DNase TURBO, Invitrogen cat#AM2238) had been added to the frozen monolayer ...
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bioRxiv - Neuroscience 2022Quote: ... donkey anti-mouse IgG, 1:500, 715-165-150, Jackson ImmunoResearch; AF488: donkey anti-mouse IgG, 1:500, A-21202, Life Technologies).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were seeded at a density of approximately 27’000 cells/cm2 into a 150 × 25 mm petri dish and were cultured for 2 weeks in William’s E medium (cat. nr.: 32551020, ThermoFisher Scientific, Massachusetts, USA) supplemented with 2 mM glutamine ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted at 1/50 or 1/150 followed by an incubation for 1h at room temperature with a secondary goat Alexa FluorTM 488 antibody (ThermoFisher Scientific, #A11029) diluted at 1/1000 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were seeded at a density of 1*106 cells per scaffold and seeding was performed dynamically [31] for 6 hours in 50 ml tubes on an orbital shaker at 150 RPM in osteogenic control medium (low glucose-DMEM (lg-DMEM, 22320, Thermo Fisher Scientific), 10% FBS (SFBS ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was generated from 150 ng of RNA in a 20 μl reaction using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, #4368814). qPCR was performed using TaqMan Fast Universal PCR Master Mix (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... transferred to a new 2.0 mL Eppendorf tube and S-Trap columns incubated with 150 µL trypsin enzyme digestion buffer (1:30 trypsin enzyme (Thermo Fisher Scientific): protein by weight ...
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bioRxiv - Physiology 2024Quote: RNA-seq was performed at the DNA Core at the CCHMC facility with 10 ng – 150 ng of total RNA used after quantification by Qubit RNA HS assay kit (Cat #Q32852; Invitrogen, Waltham, MA). Based RNA integrity value above 7 determined by the spectrofluorometric measurement RNA samples was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595 ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were separated using a 180-minute gradient on an in-house pulled C18 (Thermo Accucore, 2.6 Å, 150 μm) 30 cm column using an Easy nLC 1200 system (Thermo Fisher Scientific) running from 96% Buffer A (5% acetonitrile ...
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bioRxiv - Immunology 2024Quote: ... BM-derived culture cells (1.5 x 106) were suspended in 150 μL of T buffer included in the Neon Transfection System 100 μL Kit (Thermo Fisher Scientific). Aliquoted 108 μL T buffer containing suspended cells was mixed with 12 μL of siRNA solution (20 μM for Invitrogen siRNA ...
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bioRxiv - Biochemistry 2024Quote: ... Protein bound to the quartz membrane was washed with 150 µL binding buffer three times and digested with Pierce™ Trypsin Protease MS-Grade (Thermo Scientific) dissolved in 50 mM TEAB buffer with digestion ratio of 1:20 ...
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bioRxiv - Cell Biology 2023Quote: ... A volume of each protein extract corresponding to 14 mg protein was transferred into a new 5-mL tube containing 150 µL streptavidin beads (Thermo Fisher Scientific) that were pre-washed with extraction buffer and incubated on a rotor wheel at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% FA in H2O and then separated on a C18 Acclaim PepMap100 nano-column (75 µm ID x 50 cm, 2.6 µm, 150 Å, Thermo Fisher Scientific) at 300 nL/min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were centrifuged and the pellets were suspended in 150 ml of lysis buffer containing proteinase K (Thermo Scientific, Walter, MA, USA) followed by hot phenol extraction and a subsequent extraction of the aqueous phase with diethyl ether ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µg of the sample were injected onto an Acclaim PepMap 100 trap column (75 μm × 150 mm, C18, 3 μm, 100 Å, Thermo Scientific). After flushing the trap column for 3 min at 7 µL/min with 100 % Solvent A the sample is separated on a 40 min linear gradient by an EASY-Spray™ HPLC Columns (75 μm × 250 mm ...
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bioRxiv - Cell Biology 2022Quote: One of the two parts was fractionated on a 1 mm x 150 mm ACQUITY column, packed with 130 Å, 1.7 μm C18 particles (#186006935, Waters) using an Ultimate 3000 UHPLC (Thermo Fisher Scientific). Peptides were separated at a flow of 30 μl/min with an 88 min segmented gradient from 1% to 50% buffer B for 85 min and from 50% to 95% buffer B for 3 min ...
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bioRxiv - Cell Biology 2023Quote: ... miRNA (miRNA-NC or miR-150-3p) and Renilla luciferase (GeneChem, Shanghai, China) were transfected into HK-2 cells using Lipofectamine 3000 (Thermo Fisher Scientific) to determine whether FTH1 was a direct target of miR-150-3p ...
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bioRxiv - Cell Biology 2023Quote: ... The eluent was buffer exchanged to storage buffer (50 mM Tris pH 8 and 150 mM NaCl) using Zeba spin desalting column 7K (ThermoFisher Scientific, USA) and was flash frozen and stored at −80°C ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA samples used for loading comprised around 50–150 ng per strain (measured by Qubit 4 Fluorometer; ThermoFisher Scientific, Waltham, USA). The sequencing ran for 48 hrs using the MinKNOW software versions 2.2 ...
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bioRxiv - Physiology 2023Quote: ... Mice were anesthetized with a xylazine/ketamine (30/150 mg/kg) (WDT) prior to blood sampling and perfusion with PBS (Thermo Fisher Scientific) with heparin (50 U/mL) ...
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bioRxiv - Biochemistry 2023Quote: ... We injected the peptides in 5 μL of aqueous 0.1% FA (Solvent A) into an Acclaim PepMap RSLC C18 column (75 μm × 150 mm, 2 μm particle size, 100 Å pore size, Thermo Scientific) kept at 40 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and separated on a C18 analytical column (Acclaim PepMap RSLC column; 2 μm, 100 Å, 75 μm × 150 mm, Thermo Fisher Scientific) using a linear gradient from 5% buffer A (80% acetonitrile ...
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bioRxiv - Biophysics 2023Quote: ... 22 mm × 22 mm) and large Petri dishes (Falcon™ Bacteriological Petri Dishes with Lid, 150 mm diameter, 15 mm height) from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2023Quote: ... beads were washed four times in MCLB buffer supplemented with 150 mM NaCl and then boiled in LDS (lithium dodecyl sulfate) sample buffer (Thermo Fisher, NP0008) supplemented with beta-mercaptoethanol to elute the bound proteins.
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bioRxiv - Neuroscience 2023Quote: ... Peptides were separated on a 100 μm inner diameter microcapillary column packed with ∼20 cm of Accucore C18 resin (2.6 μm, 150 Å, Thermo Fisher Scientific). For each analysis ...
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bioRxiv - Genetics 2023Quote: Chemical selections were conducted as follows: G418 selection was conducted for 6 days in 150 μg/mL G418/culture medium (#11811023; Thermo Fisher Scientific) 2 days after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... The cover slips were then incubated with 150 µL 1:500 secondary antibody (Alexa Fluor 488-conjugated Goat anti-mouse IgG, Thermo Fisher Scientific) diluted in blocking solution in the dark at RT for 120 min ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 hours at room temperature before overnight incubation with rabbit polyclonal anti-ELAPOR1 antibody (Thermo Fisher Scientific, PA5-72691, 1:150). Cells were then washed three times with PBS for 15 min and incubated with anti-rabbit IgG (H+L ...
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bioRxiv - Developmental Biology 2023Quote: ... 15 cm dishes (CytoOne 150 x 20 mm Tissue Culture Dish) each 24 h before collection for 10 mg/mL Hoechst 33342 (Thermo Fisher Scientific) staining ...
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bioRxiv - Biochemistry 2023Quote: ... with an EASY-Spray Capillary HPLC Column (75 μm x 150 mm, 3 μm C18 particles; ES900) or an µPAC HPLC Column (200 cm, Thermo Scientific, #COLNANO200G1B) over 5-40% acetonitrile gradient at 300 nl/min flow rate ...
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bioRxiv - Bioengineering 2023Quote: ... for microultracentrifugation at an RCFmax of 140,000 x g (Sorvall MX 150+ Micro-Ultracentrifuge, ThermoFisher; S110-AT Fixed-Angle Rotor, ThermoFisher, k factor=15.4) for 1 hour at 4° C ...
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bioRxiv - Biophysics 2024Quote: ... Chromatography of peptides prior to mass spectral analysis was accomplished using a capillary emitter column (PepMap○R C18, 3 µM, 100 Å, 150 x 0.075 mm, Thermo Fisher Scientific) onto which 2 µl of extracted peptides were automatically loaded ...
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bioRxiv - Biochemistry 2024Quote: ... Protein bound to the quartz membrane was washed with 150 µL binding buffer three times and digested with Pierce™ Trypsin Protease MS-Grade (Thermo Scientific) dissolved in 50 mM TEAB buffer with a digestion ratio of 1:20 ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were separated by reverse phase HPLC on a Hypersil Gold C18 column (150 × 2.1 mm, 3 μm particle size, 175 Å pore size, Thermo Fisher Scientific) using a linear gradient (2-50% solvent B in 15 min) ...
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bioRxiv - Immunology 2024Quote: ... we co-transfected 3×106 3-day activated CD8+T cells with 150 pmol/106 cells of human TSP-4-specific siRNAs (#s14100, Thermo Fisher Scientific) and 1.5 μg/106 of the pMax-TSP-1-GFPSpark construct ...
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bioRxiv - Molecular Biology 2024Quote: ... were transfected into hTERT-HM-ERα cells at a final concentration of 30 pmol using Lipofectamine RNAi MAX reagent (Invitrogen, 13778-150) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and lysed in HN buffer (50 mM Hepes and 150 mM NaCl) with 1% triton and protease inhibitor cocktail (Thermo Fisher Scientific). Cells were incubated on a rotator for 20 min at 4°C then centrifuged for 15 min at 17,000 ×g ...
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bioRxiv - Molecular Biology 2024Quote: ... and 150 ng or 500 ng (for iPSCs) was reverse transcribed using the Superscript III Reverse Transcriptase kit (Life Technologies, ThermoFisher Scientific). Clearance of the Sendai vectors was assayed by RT-PCR amplification of fibroblast and iPSC cDNA with primers specific to the vector backbone (SEV) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 150 ng or 500 ng (for iPSCs) was reverse transcribed using the Superscript III Reverse Transcriptase kit (Life Technologies, ThermoFisher Scientific). Clearance of the Sendai vectors was assayed by RT-PCR amplification of fibroblast and iPSC cDNA with primers specific to the vector backbone (SEV) ...
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bioRxiv - Immunology 2024Quote: Polar extracts were analysed on a Accucore-150-Amide-HILIC column (100 x 2.1 mm, 2.6 μm, Thermo Fisher Scientific, MA, USA). For positive ion mode analysis ...
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bioRxiv - Plant Biology 2024Quote: ... Cell pellets were resuspended with a lysis buffer (150 mM NaCl, 10 mM HEPES, pH 7.5) containing Pierce™ Protease Inhibitor (ThermoFisher Scientific #A32965) as suggested by the manual ...
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bioRxiv - Immunology 2024Quote: ... Program T-023 with 150 pmol/106 cells of human TSP-1 and TSP-4-specific siRNAs (#s14108 and #s14100, Thermo Fisher Scientific) and control scrambled siRNAs (#4390846 ...
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bioRxiv - Microbiology 2024Quote: ... The next day cells were transfected with 50 nM of Tollip SMARTpool (Dharmacon catalog number L-016930-00-0005), or NON-TARGETplus Non-targeting Control siRNAs siRNA (Dharmacon, catalog number D-001810-01-05) using Lipofectamine RNAiMAX (Invitrogen, 13778-150). Twenty-four hours post-siRNA transfection ...
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bioRxiv - Immunology 2024Quote: ... using a C18 reversed-phase column set at 35°C (75 μm i.d.× 150 mm, Pep Map™ RSLC C18, 2μm, 100Å, Thermo Fisher Scientific, ES904) connected to a Q-Exactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Protein samples from gills and hemocytes were homogenized separately in IP lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 5% glycerin; Thermo Scientific, Shanghai, China) with a protease and phosphatase inhibitor cocktail (Merck ...
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bioRxiv - Biochemistry 2024Quote: ... at 300 nL/min was used to elute peptides from the capillary reverse-phase column (0.075 mm × 150 mm, Acclaim Pepmap 100® C18, Thermo Fisher Scientific). Eluted peptides were electrosprayed online at a voltage of 1.8 kV into the Orbitrap Elite mass spectrometer ...
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bioRxiv - Molecular Biology 2024Quote: ... with a C18 column (50 µm x 150 mm) coupled directly to a Q-Exactive plus Orbitrap mass spectrometer (Thermo Fisher Scientific). Peptides were fragmented by collision induced dissociation with nitrogen ...
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bioRxiv - Immunology 2024Quote: ... Lung was cut into pieces and digested for 1h at 37°C with 150 U/ml Collagenase type I (Gibco, 17100-017). Pieces were fully disrupted through 100µm strainers and pellet resuspended in 44% Percoll (Citiva/Fisher) ...
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bioRxiv - Immunology 2024Quote: ... (glyco-)peptides were separated using an Acclaim PepMap C18 column (particle size 2 μm, pore size 100 Å, 75 × 150 mm, Thermo Scientific) and analyzed on a quadrupole-time-of-flight-mass spectrometer (Impact HD ...
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bioRxiv - Bioengineering 2024Quote: ... and the optical cell density was measured using a GENESYS 150 UV-Vis spectrophotometer at 600nm wavelength (Thermo Fisher Scientific, MA, USA).