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Citations for Thermo Fisher :
1151 - 1200 of 1270 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Bands of interest between 150 and 500 bp were excised and purified using the GeneJET Gel Extraction Kit (K0691, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were separated on a 100 µm inner diameter microcapillary column packed with ~35cm of Accucore C18 resin (2.6 µm, 150 Å, Thermo Fisher Scientific). For each analysis ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were then separated on a C18 PepMap nano-column (75 µm ID x 15 cm, 2.6 µm, 150 Å, Thermo Fisher Scientific) with a 45-minute linear gradient from 7% to 35% buffer B (A ...
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bioRxiv - Cell Biology 2024Quote: ... was thawed on ice and diluted in Phosphate-buffered saline (PBS) at a 1:150 ratio to coat 70 mm cell culture dishes (Avantor, China) and 96-well plates (Thermo Scientific, Denmark). The coated plates were then incubated overnight at room temperature and the leftover vitronectin solution was removed before use ...
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bioRxiv - Cancer Biology 2024Quote: Snap frozen tissue samples were briefly thawed and weighed prior to 30s of mechanical homogenization (Fisherbrand homogenizer 150 using plastic generator probes, Fisher Scientific, UK), in 8 mL lysis buffer (0.02 M Tris ...
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bioRxiv - Biochemistry 2024Quote: ... samples were cooled down to room temperature and sonicated for 15 min at 150 W using an ultrasonic water bath (Fisher Scientific, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... Chromatographic separation was performed on a Dionex U3000 nanoHPLC system equipped with an Accucore C4 column (50 cm × 75 μm, 2.6 μm, 150 Å) coupled online to a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) equipped with the High-Field- Asymmetric Waveform Ion Mobility Spectrometry (FAIMS ...
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bioRxiv - Genetics 2024Quote: Muscle tissues (20 mg) were homogenized in 150 µL of RIPA buffer (WAKO) containing Halt phosphatase and proteinase inhibitor cocktail (Thermo Fisher Scientific) using an ultrasonic homogenizer (VCX-130 ...
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bioRxiv - Evolutionary Biology 2021Quote: PCR amplification of the above reaction was performed by taking 150 ng of cDNA template and adding 10 uL 5X Phusion HF buffer (Thermo Fisher Scientific F518L), 1uL 10 mM dNTPs (each) ...
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bioRxiv - Systems Biology 2022Quote: ... quartz materials (150 grams total) were added to an autoclaved 250 ml wide-mouth volatile organics analysis (VOA) glass jar (Thermo Fisher V220-0125). Samples were first manually mixed for 30 seconds by shaking and repeatedly turning the jar ...
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bioRxiv - Molecular Biology 2021Quote: ... where they were desalted online before being separated on a 50 μm x 150 mm 100 Å 2U PepMap EasySpray column (Thermo Fisher Scientific). Peptides were eluted using a 90 min gradient of 0.1% formic acid (A ...
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bioRxiv - Biochemistry 2020Quote: ... 75 μm × 150 mm) and µ-pre-column (300 μm × 5 mm) coupled online to LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific). The eluents used were ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were run on 6% TBE gel in 1 x TBE (150 V, 90 min, 4°C) stained with SYBR Gold (Thermo Fisher Scientific. S11494) and InstantBlue Protein Stain (Expedeon).
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bioRxiv - Molecular Biology 2021Quote: Fifteen milligrams of powdered human liver tissue were lysed in 200 uL of lysis buffer (8M Urea, 50 mM Tris-HCl pH7.5, 150 mM NaCl and 1X Halt™ Protease Inhibitor Cocktail (Thermo Fisher Scientific, #87786)) ...
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bioRxiv - Neuroscience 2020Quote: ... Both analytes were separated on a Hypersil™ BDS C18 column (150 mm, VDS-optilab) using isocratic flow of the mobile phase MD-TM (Thermo Fisher Scientific) at a rate of 0.5 ml/min ...
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bioRxiv - Cell Biology 2021Quote: ... and a C18 PepMap analytical column (particle size: 2 μm; pore size: 100 Å; diameter: 50 μm; length: 150 mm; Thermo Fisher Scientific) using a 40 min linear gradient (300 nL/min ...
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bioRxiv - Neuroscience 2020Quote: ... then replenished with either 200 μL (chamber slide cells) or 6 mL (150 mm dish cells) of Opti-MEM I (ThermoFisher Scientific, Cat#31985062). BioPORTER medium mix was added at a 1:1 volume ratio in the cells grown in chamber slides and a 1:3 volume ratio in the cells grown in 150 mm dishes ...
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bioRxiv - Plant Biology 2021Quote: ... The analytical column was an Acclaim™ PepMap™ 100 C18 column (75 μm × 150 mm, 100 Å, 3 μm, Thermo Scientific, USA). The mass spectrometer was operated in data-dependent acquisition mode ...
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bioRxiv - Neuroscience 2020Quote: ... For separation a reversed-phase Acclaim PepMap RSLC column (150 mm × 75 μm, particle size 2 μm, pore size 100 Å, Thermo Fisher Scientific) was used ...
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bioRxiv - Microbiology 2021Quote: ... Each siRNA including negative control siRNA at an amount of 20 pmol was transfected into cells with 6 µL Lipofectamine RNAiMAX Reagent (Life technologies, 13778-150). 24 h later ...
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bioRxiv - Biochemistry 2021Quote: ... Separation of lipids was performed on a 150 mm BETASIL Diol-100 column with a particle size of 5 μm and 0.32 mm inner diameter (Thermo Fisher Scientific, Bremen, Germany). Detailed information for LC-MS data acquisition is given in Supplementary Materials and Methods.
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bioRxiv - Immunology 2022Quote: ... The cells were co-transfected with 650 μg of SOSIP expressing plasmid DNA and 150 μg of furin expressing plasmid DNA complexed with 293Fectin (ThermoFisher Cat No. 12347019). On day 6 cell culture supernatants were harvested by centrifugation of the cell culture for 30 min at 3500 rpm ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 100 µl of whole-blood specimens (diluted in 150 µl PBS) using TRIzol LS Reagent (Invitrogen/Life Technologies/Thermo) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... 300 ng proteolytic digest using a one-dimensional shotgun proteomic approach on a nano-liquid-chromatography system consisting of an EASY nano-LC 1200 equipped with an Acclaim PepMap RSLC RP C18 separation column (50 μm × 150 mm, 2 μm and 100 Å) coupled to a QE Plus Orbitrap mass spectrometer (Thermo Scientific, Germany). The flow rate was maintained at 350 nL/min using as solvent A water containing 0.1% formic acid ...
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bioRxiv - Evolutionary Biology 2020Quote: Samples were first buffer exchanged in 150 mM ammonium acetate (NH4OAc) at pH 7.4 using 0.5 mL ZebaTM Spin desalting Columns (Thermo Fisher Scientific, Waltham, MA USA). Then ...
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bioRxiv - Pathology 2021Quote: ... Thoracic aortas were inflated by injection of optimum cutting temperature compound (150 µl, OCT, Cat# 23-730-571, Thermo Fisher Scientific, Waltham, MA) from the left ventricle using an insulin syringe with 28-gauge needle ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were collected and lysed to extract total protein and RNA using CHAPS lysis buffer (30 mM Tris-Cl pH 7.5, 150 mM NaCl, 1% CHAPS) containing 100 units/mL RNaseOUT™ (Invitrogen; Life Technologies, Burlington, ON) on ice (20 min ...
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bioRxiv - Neuroscience 2021Quote: ... antibodies, followed by incubation with Cy3 (Jackson Immuno, 715-165-150, 1:200) and Alexa Fluor 647 (Life Technologies, A-21448, 1:400) respectively ...
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bioRxiv - Immunology 2021Quote: ... Total RNA from single-sorted B cells was reverse transcribed in the original sorting plate with 150 ng Random Hexamer Primer (Thermo Scientific, Cat#S0142), 1 mM dNTP Mix (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: Heat shocks were performed by transferring batch cultures (5 ml culture volume in a 20 mm x 150 mm borosilicate glass tube (Fisher Scientific, NH, USA)) from one water bath to another (WSB-30 ...
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bioRxiv - Microbiology 2022Quote: ... lysates were resuspended in cleavage assay buffer (20 mM HEPES, 150 mM KoAC, 1 mM DTT) in the presence of protease inhibitors (ThermoFisher, cat. no. 78440). Lysates were incubated with wild type or catalytically inactive poliovirus 3Cpro (C147A ...
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bioRxiv - Developmental Biology 2022Quote: ... Electrophoresis was conducted at 150 V for 4 h on ice in NuPAGE™ MES SDS Running Buffer (20X) (Thermo Fisher Scientific, NP0002) supplied with 1x NuPAGE™ Antioxidant (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The columns were washed six times with 150 µL S-trap binding buffer and then the columns were placed into fresh Low Protein Binding Microcentrifuge Tubes (Thermo Fisher Scientific 90410). Trapped proteins were cleaved by adding 1
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bioRxiv - Neuroscience 2022Quote: ... and thereafter separated on a reversed-phase Acclaim PepMap RSLC column (length 150 mm, inner diameter 75 μm; particle size 2 μm; pore size 100 Å) (both Thermo Fisher Scientific). Mobile phases were A ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were then placed on a magnet and washed once with 150 μl of RNA-WB1 and then with 30 μl of 1x First-Strand buffer (made from 5x stock; Life Technologies 18080-044) and transferred in a new PCR strip ...
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bioRxiv - Neuroscience 2023Quote: ... cells were cultured in 150 mm dishes (VIOLAMO, AS ONE, Japan) in a culture medium containing DMEM (high glucose) (Thermo Fisher Scientific, USA), 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfections were done with 150 pmol of siRNA in complete DMEM medium for 8-10 hours using Lipofectamine™ RNAi max (ThermoFisher Scientific, 13778150). Media was changed following transfection incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... and a C18 PepMap analytical column (particle size 2 μm, pore size 100 Å, diameter 50 μm, length 150 mm, Thermo Fisher Scientific) using a 40 min linear gradient (300 nL/min ...
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bioRxiv - Genetics 2024Quote: ... the supernatant was discarded and the DNA was eluted from the magnetic beads by adding 150 μL of DNA grade H2O (Invitrogen cat no. AM9922) and incubating at 37°C for 30 min while shaking (250 rpm) ...
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bioRxiv - Immunology 2023Quote: ... Plasmid DNA expressing the envelope SOSIP and furin were co-transfected at a 4:1 ratio (650 μg and 150 μg per transfection liter, respectively) and incubated with 293fectinTM transfection reagent (ThermoFisher Cat No. 12347019) in Opti-MEM I Reduced Serum Medium (ThermoFisher Cat No ...
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bioRxiv - Biophysics 2024Quote: ... the resulting supernatant was transferred to 1.5 ml top-opening centrifuge tubes and centrifuged for 1.5 hours at 150,000 x g at 4 °C (Sorvall MX 150+ Micro-Ultrawiring, Thermo Fisher Scientific, Waltham, MA, USA) to obtain the subpopulation of small EVs.
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bioRxiv - Plant Biology 2023Quote: ... The metabolites were separated in the LC using a Hypersil gold C18 reversed-phase column (150 × 2.1 mm, 3µ particle size; Thermo Scientific, Waltham, Massachusetts, USA) held at 30 ᵒC ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were resolved on an EASY-Spray PepMap RSLC C18 column (Thermo Scientific, 25 cm x 150 μm ID, 2 μm, 55°C) with the following gradient profile delivered at 300 nl min-1 by a Dionex RSLCnano chromatography system (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were separated on a 15 cm column packed with Accucore150 resin (150 Å, 2.6mm C18 beads Thermo Fisher Scientific, San Jose, CA) using an 80 min acetonitrile gradient ...
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bioRxiv - Microbiology 2022Quote: ... equipped with an Acclaim PepMap RSLC RP C18 separation column (50 um × 150 mm, 2 µm, 100 Å) and a QE plus Orbitrap mass spectrometer (Thermo Fisher, Germany). The flow rate was maintained at 350 nL over a linear gradient from 5 % to 30 % solvent B over 65 minutes and finally to 60 % B over 20 min ...
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Antigen Geometry Tunes Mast Cell Signaling Through Distinct FcεRI Aggregation and Structural ChangesbioRxiv - Biophysics 2023Quote: ... Cells were rinsed in ice-cold PBS and lysed on ice for 30 min with NP-40 lysis buffer (150 nM NaCl, 50 mM Tris, 1% NP-40) in the presence of Halt Protease and Phosphatase inhibitors (Thermo Fisher Scientific #78446). Lysates were cleared by centrifugation at 11,000 x g for 20 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of each sample was pooled together to make a “pooled sample” for a given derivatization batch and 80 μL of each individual sample were loaded into a 150 μL glass insert (CAS#13-622-207, Thermo scientific, Florence, KY) inside of a GC vial with a 9 mm PTFE/red rubber septum (CAS#C4000-30 ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 µg of peptides were applied onto an Acclaim PepMap 100 C18 column (2 μm particle size, 1 mm ID × 150 mm, 100 Å pore size; Thermo Fisher Scientific) and separated using a two-stepped gradient ...
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bioRxiv - Molecular Biology 2022Quote: ... Briefly, cells were seeded in 150 cm2 culture flasks (TPP, cat. #90151) at 50 % confluency in serum-free DMEM (Gibco, cat. #61965-026). 15 h later ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA expressing the envelope SOSIP and furin were co-transfected at a 4:1 ratio (650 μg and 150 μg per transfection liter, respectively) and incubated with 293fectinTM transfection reagent (ThermoFisher Cat No. 12347019) in Opti-MEM I Reduced Serum Medium (ThermoFisher Cat No ...