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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... then treated with 2 U/10 μg RNA RNase- free DNase I (Invitrogen, cat. no. #AM222) at 37°C for 30 mins to remove DNA contamination ...
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bioRxiv - Molecular Biology 2023Quote: ... pellets were centrifuged and air dried for 5 min before addition of RNase-free H2O (Ambion). RNA was quantified using the Qubit BR kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from samples was isolated using RNAqueous-micro Kit including RNase-free DNase treatment (Invitrogen). Isolated RNA was reverse transcribed into cDNA using oligo (dT ...
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bioRxiv - Molecular Biology 2020Quote: ... After RT we digested all residual oligos using adding 5 µl mastermix of 4 µl nuclease free H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049), 0.5 µl of Exonuclease I (NEB #M0568 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 µl of 100 µM bottom splint (IDT) diluted in 7 µl nuclease free H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049). The reaction was annealed at 95 °C for 1m following 20 s at 95 °C and decreasing temperature by 1 °C every cycle ...
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bioRxiv - Microbiology 2019Quote: ... or RNase cocktail (0.5U RNase A + 20U RNase T1, ThermoFisher) at 37 ℃ for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... they were rinsed with MilliQ water and incubated with RNase A (25 μg/ml, cat no. EN0531; Thermo Scientific) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 °C for 10 minutes and re-suspended in 10 μL nuclease free water (Ambion, AM9937). Nuclei were then counted using a haemocytometer ...
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bioRxiv - Genetics 2019Quote: ... suspended in nuclease free water and quantified with a Nanodrop® 2000c spectrophotometer (Thermo-Fisher Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... This consisted of 10 µL/well made by adding 0.8 µL of nuclease free water (Ambion), 5 µL of LC480 SYBR Green I Master (2 X conc ...
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bioRxiv - Systems Biology 2020Quote: ... 1.65 μL of Nuclease-free water and 1 μL of Maxima H Minus RT (Thermo Scientific). Finally ...
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bioRxiv - Microbiology 2022Quote: ... using the Buffer for the <300bp cutoff and eluted in 30µL of Nuclease-free water (Ambion). The PCR products were then subjected to next generation sequencing using the Illumina NovaSeq or MiSeq platforms.
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bioRxiv - Developmental Biology 2022Quote: ... (probe sets are presented in Supplementary Table T1) and dissolved in Nuclease-Free Distilled Water (Invitrogen). HCR amplifiers with B1-Alexa Fluor-546 ...
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bioRxiv - Microbiology 2021Quote: ... Viral lysate was diluted 1:10 by adding 90 μl of nuclease-free water (Life Technologies). qRT-PCR reactions were prepared by mixing 9.6 μl of diluted viral lysate with 10.4 μl of reaction mix containing 10 μl of 2x Maxima SYBR Green/ROX qPCR Master Mix (ThermoFisher) ...
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bioRxiv - Genetics 2021Quote: ... RNA was resuspended in nuclease-free water and quantified by a Nanodrop 2000 spectrophotometer (Thermo Scientific). The quality of RNA was estimated by using the RNA 6000 Nano or Pico Bioanalyzer 2100 Assay (Agilent) ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by another 10 min incubation with 160 μL of nuclease-free water (Thermo Fisher Scientific) and reduced to 50 μL using a CentriVap Concentrator (Labconco ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from the agar plugs and eluted in 40 µl nuclease free water (Invitrogen). Reverse transcription was performed using Maxima RT (Thermofisher ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from the agar plugs and eluted in 40 μl nuclease-free water (Invitrogen). Reverse transcription was performed using Maxima reverse transcriptase (RT ...
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bioRxiv - Bioengineering 2020Quote: ... The RPA Mastermix was made by mixing 3.5 μL of Nuclease-free ultrapure water (Fisher Scientific) with the Premix ...
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bioRxiv - Bioengineering 2021Quote: ... We prepared LAMP primer mix and probe set mix stocks in nuclease-free water (Thermo Fisher) and stored at -20 °C ...
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bioRxiv - Biophysics 2021Quote: ... Ambion™ DEPC-treated nuclease-free water (0.2 µm filtered and autoclaved) was purchased from Invitrogen, Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from the agar plugs and eluted in 40 µl nuclease-free water (Invitrogen). Reverse transcription was performed with a universal IAV primer (31 ...
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bioRxiv - Microbiology 2021Quote: ... Each oligonucleotide was dissolved in nuclease-free water and quantified with NanoDrop 2000 (Thermo Fisher Scientific) at 260 nm before use.
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from the agar plugs and eluted in 40 μl nuclease-free water (Invitrogen). Reverse transcription was performed using Maxima reverse transcriptase (RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Final RNA was eluted with nuclease-free water and quantified using the NanoDrop 2000 (ThermoFisher Scientific). Three biological replicates of non-targeting control ...
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bioRxiv - Molecular Biology 2022Quote: ... The purified DNA pellet was eluted in 15-20 µL of nuclease free water (Invitrogen, 10977015) or 0.1x TE and was used for library preparation using NEBNext Ultra II DNA library prep kit for Illumina (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The product was precipitated with isopropanol and resuspended in 3 μl nuclease-free water (Invitrogen, 10977015), followed by electroporation into Endura ElectroCompetent Cells (Lucigen ...
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bioRxiv - Biochemistry 2023Quote: ... Purified RNA fragments were resuspended in nuclease-free water and quantified using Qubit 2.0 Fluorometer (Invitrogen). A fraction of RNAs (10~200 ng ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... left to dry at room temperature and resuspended in 50 μl of nuclease-free water (Invitrogen) by shaking in a thermomixer (64°C ...
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bioRxiv - Neuroscience 2023Quote: ... Primers (IDT) were diluted in nuclease-free water with PowerUp SYBR Green master mix (Applied Biosystems) and qPCR performed on the Applied Biosystems 7300 machine ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the RNA was eluted twice with preheated 50 μL of Ambion Nuclease-free water (Invitrogen) at 95 °C for 2 minutes each time ...
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bioRxiv - Microbiology 2023Quote: ... The gDNA pellet was washed with 70% ethanol twice and dissolved in nuclease-free water (Invitrogen). The gDNA concentration was measured by Qubit fluorometer.
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bioRxiv - Bioengineering 2023Quote: ... 0.25 µL of reverse primer against NT-sgRNA and 2 µL of nuclease-free water (Ambion). Mixtures were heated to 65°C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The genomic DNA in the total RNA was eliminated by digestion with RNase-free DNase (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... of around 1 and then incubated with or without 100 µg/mL DNase free Rnase A (ThermoFisher) and 50 mM EDTA at 37 °C for 30 min followed by Western blot of the FLAG-tagged peptides ...
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bioRxiv - Plant Biology 2020Quote: ... Then 20 μL 10X DNase I buffer and 2 μL RNase-free DNase I (Ambion; http://www.lifetechnologies.com) were added to each tube ...
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bioRxiv - Plant Biology 2021Quote: ... Collection was performed into 1.5 ml RNase free non-stick Eppendorf low binding tubes (Thermofisher Cat# AM12450) containing 10μl of NIB WASH ...
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bioRxiv - Plant Biology 2020Quote: ... Ten to fifty-micrometer sections were mounted in 50% glycerol onto RNase-free slides (Thermo Fisher Scientific). After adding 20μl 200μM DFHB ...
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bioRxiv - Immunology 2022Quote: ... and then resuspended in 1M buffer (Chicaybam et al., 2013) consisting of RNAse-free H2O (Life technologies), 5mM KCl (Life technologies) ...
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bioRxiv - Neuroscience 2019Quote: ... RNA was eluted in 17µl RNase free ddH2O and quantified with a Qubit 4 Fluorometer (ThermoFisher; Q33227). Paired-end RNAseq data were generated with the Illumina HiSeq 4000 platform following the Illumina protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Degradation of DNA from samples was ensured by treatment with RNase-free DNase (Invitrogen, Thermo Fisher Scientific). 1 μg of total RNA per reaction was used to synthesize cDNA with the SuperScript® VILO™ cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Degradation of DNA from samples was ensured by treatment with RNase-free DNase (Invitrogen, Thermo Fisher Scientific). 1 μg of total RNA per reaction was used to synthesize cDNA with the SuperScript® VILO™ cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μg of total RNA were digested with 2U of RNase free DNase (Thermo Scientific, MA, USA) for 30 min at 37° C ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was removed with DNase I RNase-free (Ambion™/Thermo Fisher Scientific, Waltham, Massachusetts, US), and cDNA synthesis carried out using a SuperScript™IV Reverse Transcriptase Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: RNA samples were first treated at 37°C for 30 min with RNase-free DNase I (Invitrogen) in 50 μl reactions containing about 5μg RNA and 1 unit DNase I ...
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bioRxiv - Plant Biology 2023Quote: ... RNA (1 µg) from each sample was treated with Ambion® DNase I (RNase-free; Applied Biosystems) in a 20 µl reaction mixture for 90 min at 37 °C to remove remaining DNA ...
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bioRxiv - Bioengineering 2022Quote: ... 1000 ng of total RNA was treated with RNAse-free DNAse I (ezDNAse from Thermo Fisher Scientific) and then reverse transcribed using SuperScript IV Vilo reverse transcriptase (Thermo Fischer Scientific ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... We added Isopropanol (650 μL) and Glycogen blue (1 μL; RNAse-free, Invitrogen, 15 mg/mL, #AM9516) then vortexed and incubated overnight at -20 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted material was then subjected to RNase A (DNase and protease-free Thermo Fisher Scientific; #EN0531) and Proteinase K (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... To digest RNA, 0.5 µL of RNase A (DNase and protease-free, 10 mg/mL) (Thermo Fisher Scientific ...