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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 1% mouse recombinant EGF (Invitrogen, 5% Recombinant human R-spondin (Peprotech).
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bioRxiv - Molecular Biology 2024Quote: ... recombinant EGF (Thermo Fisher Scientific), or EGF+ phage (M13SW7-EGF ...
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bioRxiv - Immunology 2021Quote: ... IFNγ levels were measured in the supernatant of the WBA using IFN gamma ELISA Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 1 week at a density of 106/well in 6 well plates with 50 ng/ml recombinant human macrophage colony stimulating factor (M-CSF) (Life Technologies, Carlsbad, CA), 1x GlutaMAX-I (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... and a combination of 18 µg/mL recombinant human Laminin 521 (Biolamina, Cat. # LN521) and 5 µg/mL Fibronectin (Gibco, Cat. # 33016-015). The Matrigel coating consisted of a 1:52 dilution of growth factor-reduced Matrigel (Corning ...
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bioRxiv - Neuroscience 2024Quote: ... All iPSC lines were subsequently maintained as previously described44 in feeder-free cultures on 0.6 µg/cm2 recombinant human laminin-521 (LN521, Biolamina)-coated cell culture plates in Essential 8™ Flex medium (E8 flex, Thermo Fisher Scientific). IPSCs were passaged enzymatically with TrypLE™ Select Enzyme and Defined Trypsin Inhibitor (both from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... the media was replaced with 100 µL of fresh 2:1 mixed media supplemented with 100 ng mL−1 recombinant human vascular endothelial growth factor (Thermo Fisher Scientific PHC9394). The samples were cultured for another 2 days before fixation with 40 mg mL−1 formaldehyde in PBS for 40 min and then washed 3 times with PBS ...
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bioRxiv - Immunology 2021Quote: ... RBD-His and Spike-his containing supernatants were batch purified using the HisPur™ Ni-NTA Resin (ThermoFisher Scientific). Supernatants were incubated with 6 ml of resin for 1h at RT ...
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bioRxiv - Microbiology 2020Quote: Recombinant expressions of proteins involved in this study were performed by using the ExpiCHO™ expression system (Thermo Scientific, A29133). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) or CaptureSelect™ IgA Affinity Matrix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Recombinant E protein was produced by transient transfection of Expi293F cells using a ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatants were harvested 4 days after transfection and then concentrated before exchange into 2× PBS at pH 6.5 and finally into 2× PBS at pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: Ribonucleoprotein complexes containing 3.9 picomoles of ATF3 sgRNA guides and 3 picomoles of purified recombinant Cas9 protein (Synthego) were transfected into HEK293T cells using Lipofectamine CRISPRmax (Invitrogen). 72 hours post transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were sonicated and recombinant proteins purified in a single step on Ni-agarose column according to manufacturer instructions (Invitrogen). Protein samples were dialyzed against PBS with 5 mM MgCl2 and 0.1% glycerol for 2h and used immediately in experiments ...
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: A baculovirus expression system was used for the expression of DUB2 recombinant protein in sf9 insect cells as described in the Bac-to-Bac Baculovirus Expression protocol (Invitrogen). The gene was first cloned into the PFastBacNKI-his-3C-LIC vector using Ligation Independent Cloning (LIC) ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... LPS-primed THP-1 cell lysates or purified recombinant NLRP3 protein were pre-cleaned using streptavidin magnetic beads (88816, ThermoFisher) to remove nonspecific binding ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Molecular Biology 2023Quote: Recombinant Rpb4/Rpb7-6His protein (5 μg) was incubated in a 50 μL slurry of HisPur Cobalt Resin (Thermo Scientific) in binding buffer (20 mM Tris [pH 7.5] and 50 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant Breg and GFP protein was labeled with membrane non-permeable biotin according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... Recombinant protein was expressed in 8L culture of Spodoptera frugiperda SF9 cells using the Bac-to-Bac system (Thermo Fisher). Cells were cultured at 27°C ...
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bioRxiv - Plant Biology 2022Quote: ... NiHa-OSR and NoFa-ICL: Fractions containing recombinant protein were pooled and dialyzed using a Slide-A-Lyzer (ThermoFisher Scientific) cassette (20 kDa MWCO ...
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bioRxiv - Cell Biology 2020Quote: ... anti-S-tag (Thermo Scientific, #MA1-981), anti-TMEM43 (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... mouse anti-V5-Tag (46-0705, Invitrogen), mouse anti-Myc-Tag (9B11 ...
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bioRxiv - Neuroscience 2020Quote: ... tag from pcDNA3.1-nV5-DEST vector (Invitrogen). To generate AP2M1 or LRRK2 single or multiple mutants ...
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bioRxiv - Microbiology 2022Quote: ... anti-FLAG tag (Thermo Fisher (PA1-984B)).
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bioRxiv - Microbiology 2021Quote: ... or DYKDDDDK Tag Monoclonal Antibody (FG4R) (Invitrogen) (1 ...
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bioRxiv - Biochemistry 2021Quote: ... and V5-tag (R96025) from Life Technologies.
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bioRxiv - Microbiology 2020Quote: ... anti-KDDDDK Tag antibody (Invitrogen, MA1-91878), Monoclonal antibody anti-GAPDH produced in mouse (SIGMA ...
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bioRxiv - Immunology 2021Quote: ... AccuPrime Tag DNA polymerase High Fidelity (Invitrogen) with initial denaturation at 94 °C for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... or anti S-tag (MA1981, Life Technologies). Proteins were detected using horseradish peroxidase (HRP)-conjugated goat anti-rabbit ...
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bioRxiv - Cell Biology 2022Quote: ... V5-tag antibody (Cat # R960-25, Invitrogen), GST antibody (Cat# 2622S ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-Strep-tag II (NC9261069, Thermo Fisher), anti-FLAG epitope tag (L00018 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies include: anti-V5 Tag (Invitrogen, ref R96025 ...
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bioRxiv - Genetics 2024Quote: ... The FISH Tag DNA Multicolor Kit (Invitrogen) was used for nick translation and chemical labelling with Alexafluor (AF ...
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bioRxiv - Cell Biology 2023Quote: ... anti-V5 tag (Invitrogen, catalog no.R960-25).
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bioRxiv - Cell Biology 2023Quote: ... HA tag (Invitrogen, MA5-27915, 1:1000), or cyclophilin B (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... V5 tag (R960-25, from Thermo Fisher), Nrf1 (this specific antibody made in our own laboratory [40]) ...
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bioRxiv - Cancer Biology 2024Quote: ... Myc-tag (Thermo Fisher Scientific, 13-2500), BAX (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... the HA tag (Invitrogen 26183, 1:100), or the influenza NP (BioRad MCA 400 or Invitrogen PA5-32242 ...
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bioRxiv - Molecular Biology 2024Quote: ... tandem mass tag (TMT) labeling (Thermo Fisher), and quantitative mass spectrometry (Ting et al. ...
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bioRxiv - Pathology 2024Quote: For tandem mass tag (TMT, Thermo Scientific) six-plex labelling ...
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bioRxiv - Biochemistry 2021Quote: ... This construct was expressed as a secreted protein by a stable Drosophila S2 cell line (30) and affinity purified using C-tag affinity resin (Thermo Fisher Scientific) followed by a size-exclusion chromatography polishing step in 20 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... Protein mixtures (three replicates for each condition) were digested and labeled by tandem mass tags (TMT) 6plex reagent (Thermo Fisher Scientific, 90061), according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Recombinant S and RBD trimeric proteins secreted in cell culture supernatants were purified using the CaptureSelect™ C-tag Affinity Matrix (ThermoFisher Scientific). Concentration of purified recombinant Abs were determined by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... transgenes were recombined with the Drosophila Gateway Vector Collection (DGVC) destination vectors expressing the N terminal tag fused target proteins under Actin5C promoter (Invitrogen, Carlsbad, CA). In addition ...