Recombinant Human PPAR gamma Protein His tag citations for Thermo Fisher

Characterization of protease activity of Nsp3 from SARS-CoV-2 and its in vitro inhibition by nanobodies

Lee A. Armstrong, et al., bioRxiv - Biochemistry 2020

Quote: ... 3ul of anhydrous ACN and 4 ul of TMT reagent (32ug of TMT reporter tag) of each TMT reporter tag (TMT 10 plex kit, Product number: 90110 and A37725., Thermo Fisher Scientific) was added to each sample and incubated at room temperature on a Thermo mixer with a gentle agitation (600 rpm ...

Immunogenicity and efficacy of the COVID-19 candidate vector vaccine MVA SARS 2 S in preclinical vaccination

Alina Tscherne, et al., bioRxiv - Microbiology 2021

Quote: ... Permeabilized cells were probed with a monoclonal antibody against the HA-tag epitope (1:1000; HA Tag mAb 2-2.2.14, Thermo Fisher Scientific, Planegg, Germany) to detect SARS-2-S protein ...

A family of bacterial Josephin-like deubiquitinases with an unusual cleavage mode

Thomas Hermanns, et al., bioRxiv - Biochemistry 2024

Quote: ... PcJOS70-324 and USP2258-605 were cloned with an N-terminal FLAG (DYKDDDDK) tag and MLKL1-180 with a C-terminal FLAG-tag into pcDNA™5/FRT/TO vector (Invitrogen™).

TGFβ signaling in cancer-associated fibroblasts drives a hepatic gp130-dependent pro-metastatic inflammatory program in CMS4 colorectal cancer subtype

TJ Harryvan, et al., bioRxiv - Cancer Biology 2024

Quote: ... 50 ng/ml recombinant mouse EGF and 100 ng/ml recombinant murine Noggin (all from Thermo Fisher Scientific). Organoids were passaged 3 times a week depending on culture confluence.

A novel adhesive complex at the base of intestinal microvilli

Christian Hartmann, et al., bioRxiv - Cell Biology 2021

Quote: In vitro binding experiments were performed with recombinant GST fusion proteins purified from E.coli and immobilized on glutathione-Sepharose 4B beads (Life Technologies #17-0756-01). Purification of GST fusion proteins was performed as described (Ebnet et al. ...

A cyclin-dependent kinase 5-derived peptide inhibits Cdk5/p25 activity and improves neurodegenerative phenotypes

Jinsoo Seo, et al., bioRxiv - Neuroscience 2020

Quote: ... The same recombinant Cdk5/p25 protein used for the MST assays was labeled with biotin using EZ-LinkNHS-PEG4-Biotinylation Kit (Thermo Scientific, Waltham, MA) and excess reagent was removed with a spin desalting column as per the manufacturer’s instruction ...

Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine induces equivalent preclinical antibody titers and viral neutralization to recovered COVID-19 patients

Paul F. McKay, et al., bioRxiv - Immunology 2020

Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...

Intramolecular synergism and bacterial lysis regulation can explain the complex multi-domain architecture of the bacteriophage endolysin PlySK1249

Frank Oechslin, et al., bioRxiv - Microbiology 2020

Quote: ... recombinant proteins were purified by affinity chromatography as described previously (27) and loaded on NuPAGE 4-12% BisTris gels (Invitrogen, Carlsbad, CA, USA) to confirm the correct molecular weights and assess their purity.

A murine model of the human CREBRF^R457Q obesity-risk variant does not influence energy or glucose homeostasis in response to nutritional stress

Jitendra S. Kanshana, et al., bioRxiv - Genetics 2021

Quote: ... Liver TAGs (Infinity Triglycerides Liquid Stable Reagent; Thermo Scientific) were determined following tissue lipid extraction in chloroform:methanol (2:1) ...

Perturb-map enables CRISPR genomics with spatial resolution and identifies regulators of tumor immune composition