Labshake search
Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... GST-tag (ThermoFisher Scientific #8-326), HIS-tag (Sigma-Aldrich H1029) ...
-
bioRxiv - Neuroscience 2022Quote: ... anti-V5 tag (Thermo Fisher, 460705), anti-TIA1 (Proteintech Group ...
-
bioRxiv - Plant Biology 2022Quote: ... Tandem Mass Tag (TMT, Thermo Scientific) labeling was performed on purified peptides from each sample as previously reported (Song et al. ...
-
bioRxiv - Cancer Biology 2022Quote: ... or HA tag (#71-5500, Invitrogen) for 16–24 hours at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... and anti-HA tag (Invitrogen, 26183) primary antibodies were prepared in 3 % Bovine Serum Albumin ...
-
bioRxiv - Cancer Biology 2023Quote: ... V5 tag (Life Technologies, cat R96025), FLAG tag (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-V5 Tag (46-0705, Invitrogen), anti-6X His Tag (ab18184 ...
-
bioRxiv - Biochemistry 2023Quote: ... 6X Tag monoclonal antibody (HIS.H8, ThermoFisher) and anti-HA antibody (made in rat ...
-
bioRxiv - Neuroscience 2024Quote: ... and the Flag-tag (Thermo Fisher Scientific Cat# MA1-91878-D488 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... V5 Tag Monoclonal Antibody (451098, Invitrogen), Human c-Myc PE-conjugated Antibody (IC3696P ...
-
bioRxiv - Biochemistry 2021Quote: ... the N-terminal His-tagged protein was purified in a single step protocol using HisPur Ni-NTA resin (Thermo Fisher Scientific), equilibrated at RT ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell lysates were cleared by centrifugation at 20,000 rpm for 45 min and the His-SUMO fusion proteins were enriched on Ni-NTA beads (Thermo Fisher Scientific). The protein was eluted using a buffer containing 25 mM Tris (pH 8.0) ...
-
bioRxiv - Cancer Biology 2023Quote: ... The 6X His tagged extracellular domain of hPD-L1 WT or 2HA proteins were expressed in the ExpiCHO cell system (ThermoFisher Scientific) and purified by the Ni-NTA agarose (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2024Quote: Purified Sl-Enc1/His-Sl-Enc2 protein samples were analyzed via SDS-PAGE with a 14% Novex Tris-Glycine Gels (Thermo-Fisher Scientific). The SDS-PAGE gel was stained with Coomassie and the overexpressed bands at the correct molecular weights were excised and stored in water ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The His-tagged proteins were purified via immobilized metal affinity chromatography (IMAC) using HisPur™ Ni-NTA resin (Thermo Scientific, USA), then eluted in 50 mM sodium phosphate ...
-
bioRxiv - Biochemistry 2024Quote: ... the purified labeled proteins were added together in 1x PBS at room temperature before adding them to Dynabeads Protein A labeled with either FLAG- or His- antibodies according to the manufacturer’s recommendations (LIFE Technologies, #10006D). First ...
-
bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
-
bioRxiv - Evolutionary Biology 2022Quote: ... and anti-mouse IgG (gamma 1) Alexa Fluor 488 (goat polyclonal, Invitrogen, AB_2535764, 1:2000) were used as secondary antibodies ...
-
bioRxiv - Microbiology 2021Quote: The HA pull down was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against HA (Invitrogen). In order to perform the pull down ...
-
bioRxiv - Microbiology 2022Quote: Recombinant proteins were produced in transiently transfected suspension culture of FreeStyle 293 cells (Thermo Fisher Scientific, Waltham, MA, USA), as previously described 66 ...
-
bioRxiv - Immunology 2021Quote: The recombinant chimera CD14-Fc protein was biotinylated using the EZ-Link Sulfo NHS-LC-LC-Biotin kit (Thermofisher) and a 5-fold molar excess of biotin ...
-
bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were affinity purified by overnight binding to HisPur Cobalt Resin (Thermo Fisher Scientific, U. S. A.) in buffer A (50 mM Tris-HCl (pH 7.6) ...
-
bioRxiv - Immunology 2020Quote: ... The vector was linearized with BglII and transformation of yeast (Pichia pastoris) and induction of recombinant protein expression was carried out according to the manufacturer’s instructions (Invitrogen). Recombinant HELMCC protein was purified from yeast culture supernatants by ionexchange chromatography using Ni2+ affinity chromatography (Amersham Biosciences) ...
-
bioRxiv - Microbiology 2020Quote: ... Purified soluble recombinant proteins were tested for bacterial endotoxins using the Limulus Amebocyte Lysate (LAL) assay kit (Invitrogen, USA).
-
bioRxiv - Biochemistry 2022Quote: ... Pierce™ recombinant protein A/G and clear round-bottom immuno nonsterile 96-well plates were obtained from ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: recombinant biotinylated sE-cvD protein was expressed at 28°C using an ExpiFectamine 293 transfection kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
-
bioRxiv - Molecular Biology 2024Quote: ... Purity of recombinant proteins was verified by SDS-PAGE with staining by GelCode Blue stain reagent (Thermo Fisher scientific).
-
bioRxiv - Neuroscience 2024Quote: ... A polyclonal antibody to Drosophila tau was prepared in rabbits immunized with full length recombinant tau protein (Thermo Fisher) and was used at 1:5,000,000 for western blotting ...
-
bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
-
bioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) as previously described30 using HisPur cobalt resin (Thermo Scientific). Before elution ...
-
bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
-
bioRxiv - Biochemistry 2023Quote: ... The coding sequences of the recombinant proteins were then inserted into the BamHI and EcoRI sites of the pcDNA3.1(+) vector (Invitrogen), and all clones were verified through DNA sequencing.
-
bioRxiv - Immunology 2024Quote: ... GPI-RBD-hGM-CSF recombinant fusion protein and GPI-hIL-12 were affinity purified from CHO-S cells (Invitrogen) stably expressing the proteins using anti-human GM-CSF (clone BVD2-21C11 ...
-
bioRxiv - Biochemistry 2024Quote: ... Cleavage of recombinant protein was verified by running sample on a Bis-Tris 4-12% gel (Thermo Fisher, NP0321), resolved by electrophoresis in 1x MOPS SDS running buffer (Thermo Fisher ...
-
bioRxiv - Immunology 2024Quote: Recombinant MAYV E2 protein (Native Antigen) was absorbed overnight at 4°C on Maxisorp immunocapture ELISA plates (Thermo Scientific) in a sodium bicarbonate buffer pH 9.3 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Recombinant lentivirus used to infect SW620 cells for protein expression was generated by the ViraPower Lentiviral Expression System (Invitrogen) according to the manufacturer’s instruction.
-
bioRxiv - Biochemistry 2024Quote: ... Recombinant SBP-tagged protein was incubated at 4°C for 30 minutes with MyOne Streptavidin T1 Dynabeads (Thermo Scientific) at multiple concentrations (SBP-MSI2 ...
-
bioRxiv - Developmental Biology 2021Quote: ... containing 500 μm disk patterns were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in pre-warmed DPBS (Thermo Fisher Scientific) for 3 hours at 37°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... ESCs were dissociated with TrypLE Express for 5 mins at 37°C and induced into EpiLCs by addition of human recombinant basic fibroblast growth factor (bFGF) (Invitrogen, 13256-029) and activin A (Peprotech ...
-
bioRxiv - Cell Biology 2021Quote: ... Phages displaying human Fabs were enriched after three rounds of biopanning on biotinylated recombinant human ACE2 immobilised to streptavidin Dynabeads (Dynal M-280, Invitrogen, cat # 112.06D). After the third round of panning ...
-
bioRxiv - Microbiology 2020Quote: ... To starve Chlamydia of tryptophan HeLa cells were incubated for 24 h in media containing 2 ng/ml recombinant human IFN-γ (Invitrogen: PHC4033) prior to infection ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were pulsed in starvation buffer supplemented with 0.1% w/v bovine serum albumin at 4 °C for 40 min with 50 ng/mL human recombinant EGF (Thermo-Fisher, Gibco™, PHG0311L) to allow ligand bind to the EGFR ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.055 mM β-mercaptoethanol (#21985-023) and recombinant human full-length bFGF (#PHG0261) at 10 ng/ml (all reagents from Life Technologies).
-
bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
-
bioRxiv - Cell Biology 2024Quote: ... DD5A)29 and three controls (Ctl1B, Ctl7C, Ctl8F)47 were cultured on human recombinant laminin LN521 (Biolamina) in Essential-8TM medium (Thermo Fisher Scientific) and passaged using Trypl Express (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: Memory Th cells were incubated in a complete culture medium with 100 IU/mL recombinant human IL-2 (Fisher Scientific, Ottawa, ON) for 24 hrs in a 5% CO2 at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... Desalted peptides were labeled with Tandem Mass Tags (TMT) using TMTPro-16plex isobaric tags (ThermoFisher Scientific; #A44520) as per manufacturer’s instructions ...
-
Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: Tandem mass tag (TMT) labelling was performed using the TMT10plexTM Mass Tag Labelling Kit (Thermo Fisher Scientific) with minor modifications from the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... The C-tag was detected using CaptureSelect Alexa fluor 488 anti-C-Tag (Thermo Fisher Scientific #7213252100) and the SPOT tag was detected using SPOT-Label Alexa Fluor 568 (ChromoTek #ebAF568) ...