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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was captured on a Ni2+-NTA HisPur® affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... Expression and purification of recombinant proteins were performed according to manufacturer’s instructions (Invitrogen Corporation, Catalog no. K1710-01): YPD medium supplemented with 100 μg ml−1 zeocin was used for initial growth of P ...
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bioRxiv - Biochemistry 2020Quote: The soluble recombinant protein was captured on a Ni2+-NTA affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with 25 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... Dinutuximab and dinutuximab LALA-PG were then purified using recombinant protein A-Sepharose 4B (ThermoFisher Scientific, Waltham, MA), buffer exchanged into antibody buffer (20mM L-Histidine ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant Endocan and PDGF-BB were labeled with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher Scientific) and Alexa Fluor™ 647 Microscale Protein Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... were used to produce recombinant NS1 proteins and were maintained in FreeStyle 293 Expression medium (Thermo Fisher Scientific) at 37°C and 8% CO2 at a density of 0.15×106 − 1.2×106 cells/mL on a cell shaker at 135 rpm ...
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bioRxiv - Biochemistry 2024Quote: ... 3 mg of protein was subjected to immunoprecipitation using ISG15 antibody (Recombinant rabbit monoclonal antibody: ThermoFisher scientific: 7H29L24). Lysates were incubated for 1 hr with the antibody on a rotor at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Both recombinant LPS (100ng/ml) and recombinant IFNy (10 ng/ml, Gibco) were provided to cells for differentiation to classical macrophages ...
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Cell Biology 2020Quote: ... coated overnight with 10 μg/ml of xeno-free human recombinant Laminin 521 (LN521, Biolamina) prepared in GMP DPBS+/+ (A1285801, Life Technologies) by reversing the strainer and washing the EBs into the plate with GMP N2 media (CTS™ DMEM-F12 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were treated with 30 ng/ml of VEGF (Recombinant Human Vascular Endothelial Cell Growth Factor, Fisher Scientific 10690920, Bordeaux, France) for 24 hours.
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bioRxiv - Immunology 2021Quote: Purified CD8+ cells were cultured in AIMV medium supplemented with 10% FBS and 100 U/ml recombinant human IL-2 (ThermoFisher Scientific) and used for sorting of CD8+ T cell subsets and for further experiments three days after isolation.
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bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ & mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ and mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Neuroscience 2024Quote: ... 6-well plates were coated with 20 µg/mL Human recombinant laminin-521 (Biolaminin; Biolamina; LN521-05) in 1x dPBS++ (Gibco; 14040117) and incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
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bioRxiv - Cancer Biology 2023Quote: H357 and H376 cells at ∼80 % confluence were treated either with distilled water (as control) or with 20 ng/ml of Human Recombinant FGF2 (PHG0026, ThermoFisher Scientific) for 48 h ...
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bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Neuroscience 2024Quote: ... followed by coating with 5 µg/mL of human recombinant laminin 521 (BioLamina, #LN521-02) in DPBS (with calcium and magnesium, Gibco #14040117) overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... They were cultured in Keratinocyte serum-free medium (SFM) supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (17005042, Life Technologies) at 37°C with 5% CO2 ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were detected by Coomassie Blue staining or Western blotting using anti-His6-tag antibodies (Thermo Fisher Scientific MA1-21315).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein with an N-terminal His10 tag was expressed in Sf9 cells following the Bac-to-Bac baculovirus system (Invitrogen). Cells were collected and solubilized to extract protein in the buffer containing 25 mM MES pH 6.0 ...
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bioRxiv - Immunology 2022Quote: ... Gamma and Omicron NTD mutants with an N-terminal human IL-2 or Mu phosphatase signal peptide and a C-terminal polyhistidine tag followed by an AviTag were used to express soluble NTD proteins by transiently-transfecting Expi293F cells (GIBCO). After four days ...
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bioRxiv - Microbiology 2020Quote: ... The HA-tagged proteins were captured with the Pierce™ HA-Tag Magnetic IP/Co-IP Kit (Thermo Scientific™). Magnetic beads (0.25 mg ...
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bioRxiv - Molecular Biology 2021Quote: ... truncation and a TEV protease cleavage site followed by a double maltose-binding protein-(MBP) tag at its C terminus was cloned into the pFastBac vector (Invitrogen). To obtain a SV1–Gs complex with good homogeneity and stability ...
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bioRxiv - Immunology 2021Quote: Protein Digestion and Tandem Mass Tag (TMT) labeling: The Pierce detergent compatible Bradford assay kit from Thermo Fisher (Winnipeg, MB) was used to determine the protein concentration ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 16 hours at 4°C with HA Tag-precoated Dynabeads-Protein G (Invitrogen, Cat.#: 10007D). Then the Dynabeads were rinsed with a high salt buffer (50mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... cyclin D1 and CDK4 and common contaminant proteins (CRAPome/Strep tag (38)) using the Proteome Discoverer 2.5 software (Thermo Fisher). The following parameters were used ...
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bioRxiv - Cancer Biology 2024Quote: ... HAUb-FLAGXPO1 conjugation and total FLAGXPO1 proteins were immunodetected by using primary antibodies for HA tag (Thermo Fisher Scientific, #26183) or FLAG M2 tag (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Recombinant B18R (Life Technologies) was used at 1ug/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Recombinant PAK6 (ThermoFisher, #PR5307A) was diluted in blocking buffer and incubated with the arrays at room temperature (RT ...
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bioRxiv - Biophysics 2023Quote: Full-length human MRS2 (Uniprot Q9HD23) conjugated with a FLAG tag at the C-terminus was expressed in Expi293F cells (Thermo Fisher Scientific, A14527). Cells at a density of 2.0-3.0 × 106 cells/ml were transfected with the MRS2-encoding pCMV plasmid using polyethylenimine (PEI ...
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bioRxiv - Molecular Biology 2024Quote: Human NRL constructs in pcDNA™4/HisMax vector containing an N-terminal Xpress tag (Cat. No. V86420, ThermoFisher Scientific, Waltham, MA, USA) or in pGEX-4 T2 plasmid containing an N-terminal GST tag (GE Healthcare/Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ApoE and sAPP were measured in human CSF samples using the Apolipoprotein E Human ELISA Kit (#EHAPOE) and Amyloid Precursor Protein Human ELISA Kit (#KHB0051) kits respectively from Life Technologies, following the manufactureŕs protocol in duplicates using a 1:100 dilution of CSF.
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bioRxiv - Biochemistry 2023Quote: Proteins from human cell lines were extracted in IP lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... RBD proteins were expressed in-house in Expi293F human cells (Thermo Fisher Scientific) by transfection of the cells with purified DNA and polyethylenimine (PEI) ...
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bioRxiv - Immunology 2021Quote: ... V5 tag-152Sm (Thermo Fisher Scientific), anti-DYKDDDDK (FLAG ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-myc-tag (Invitrogen, R950-25), zn5 (Oregon Monoclonal Bank ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-V5 tag (Invitrogen, R96025), rabbit anti-ZNF598 (Thermo Fischer Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Tandem Mass Tag (TMT, Thermo Scientific) labeling was performed on 330 μg of purified peptides from each sample as previously reported (Song et al. ...
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bioRxiv - Biochemistry 2022Quote: ... mouse monoclonal anti-his6 tag (Thermofisher) 1/10,000 dilution ...