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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
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bioRxiv - Immunology 2023Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Biochemistry 2023Quote: Recombinant trimeric spike proteins (5 µg/mL) were plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then transfected overnight with 100 pmol of tracrRNA and crRNA complexed with 4 pmol recombinant cas9 protein using RNAiMAX (Thermo Fisher). Medium was then replaced and cells were grown for 48 h before assessing fluorescence and sorting.
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bioRxiv - Microbiology 2024Quote: Recombinant proteins were transiently expressed in Expi293F™ cells cultured in suspension using the Expi293™ expression system kit (ThermoFisher Scientific) in the presence of the 1 μM kifunensine ...
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bioRxiv - Cancer Biology 2024Quote: ... Organoids cultured for 72 hours in ENR media supplemented with recombinant Wnt surrogate FC fusion protein (5 nM, Thermo Fisher Scientific), Rho-associated protein kinase (ROCK ...
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bioRxiv - Biochemistry 2024Quote: ... 0.2 μM of biotinylated recombinant LacR proteins were immobilized on one volume of streptavidin-coated magnetic beads (Dynabeads M-280, Invitrogen 11206D) in six volumes of binding buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection efficacy was confirmed by western-blot measurement of the expression of the HA-tagged fusion proteins with the HA tag monoclonal antibody (Thermo Fisher Scientific, USA).
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bioRxiv - Microbiology 2021Quote: ... Cdk1 and its associated proteins were co-purified using the Pierce™ c-Myc-Tag IP/Co-IP Kit (Thermo Scientific, Rockford, IL). Cell lysates from the CDK1-Myc strain were used as a negative control ...
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bioRxiv - Biochemistry 2021Quote: ... NiV RdRp P/L complex was purified using Ni-NTA affinity chromatography based on the P protein N-terminal eight-histidine tag according to the manufacturer’s specifications (Thermo Scientific, Rockford, IL, USA). The protein identities of the purified HCV nsp5b RdRp and NiV RdRp P/L complex were confirmed by mass spectrometry (MS ...
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bioRxiv - Cancer Biology 2020Quote: Equal amounts of protein were removed from each sample and labelled using a TMT10plex or TMT11plex Mass Tag Labelling Kit (ThermoFisher, Catalog Number A34808). TMT labelling efficiency was measured by LC-MS3 analysis after combining equal volumes (equivalent to ~ 1 μg each ...
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bioRxiv - Molecular Biology 2022Quote: ... together with a captured protein expression plasmid (MS2-CP) containing a FLAG tag for 48 hours by using Lipofectamine™ Reagent and PLUS™ reagent (ThermoFisher Scientific) (Fig ...
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bioRxiv - Molecular Biology 2023Quote: ... Tandem Mass Tag 6-plex labelling of eluted RNA-binding proteins was performed using the TMTsixplex Isobaric Mass Tagging Kit (Thermo Scientific; Rockford, IL) and analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Guanosine-5-diphosphate (GDP) and guanosine 5’-O-[gamma-thio]triphosphate (GTPγS) were purchased from Fisher Scientific and Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
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bioRxiv - Cell Biology 2020Quote: ... the strips were washed 3 times in PBS-T before incubation in primary antibody (GST Tag Mouse anti-Tag, Clone: 8-326, Invitrogen) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... with a C-terminal Strep tag for purification and a foldon tag for trimerization was inserted into the pFastBac-Dual vector (Invitrogen) and was expressed using Bac-to-Bac baculovirus system (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... with a C-terminal Strep tag for purification and a foldon tag for trimerization was expressed in FreeStyle 293-F cells (Invitrogen). The plasmid was transiently transfected at a density of 2 × 106 cell per ml using polyethyleneimine (PEI ...
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bioRxiv - Molecular Biology 2020Quote: ... ORFs were cloned in pDEST15 (containing the GST tag) and pDEST17 Gateway (containing the His6 tag) vectors (Thermo Fisher Scientific). For Y2H assays ...
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bioRxiv - Neuroscience 2024Quote: ... Full-length mouse GPR158 containing an N-terminal HA-tag or smURFP-tag were cloned into pcDNA3.1 (Thermo Fisher Scientific). Full-length cDNA encoding mouse PLCXD2 containing a C-terminal FLAG-tag in pcDNA3.1 (clone OMu06191 ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide, Applied Biosystems) and incubated at room temperature for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems]) and incubated at room temperature for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cry1 pcDNA4A-myc-his and Cry2 pcDNA4A-myc-his plasmids were double digested with NotI and XhoI FastDigest enzymes (Thermo Scientific) and used as host plasmids ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of pET28a-his TEV-glnR or pET28a-his TEV-glnR derivative was firstly transformed into expression strain BL21(DE3) (Invitrogen, Inc.). then single colonies of the positive transformants were inoculated and amplified with 5 L LB broth supplemented with 50 μg/ml kanamycin at 37 °C with shaking ...
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bioRxiv - Cell Biology 2020Quote: ... 25ng/mL recombinant murine EGF (Gibco), 50ng/mL recombinant human HGF (Peprotech) ...
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bioRxiv - Microbiology 2022Quote: ... and RNAseOUT recombinant ribonuclease inhibitor (Invitrogen). Next ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant IGF1 (Life Technologies, cat# PHG0071) and picropodophyllin (PPP ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant vitronectin (A14701SA, Thermo Fisher) was diluted in DPBS at the concentration of 1:200 to get the final concentration of 2.5 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant TNFα (Thermo Fisher, cat# PHC3015) was reconstituted at 0.1mg/ml in water ...
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bioRxiv - Cell Biology 2023Quote: ... mouse recombinant Egf (Life Technologies #PMG8043), 100 ng/mL mouse recombinant Noggin (Peprotech #250-38 ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant DKK1 was purchased from Gibco through Fisher (catalog number PHC9214) ...
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bioRxiv - Cancer Biology 2024Quote: ... and recombinant mouse IFNγ (PMC4031, Gibco). PARP14 inhibitor RBN012759 [7] and PARP14 PROTAC RBN012811 (Ribon Therapeutics ...
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bioRxiv - Microbiology 2020Quote: ... DYKDDDDK Tag mouse monoclonal antibody (FG4R) (Thermo Fisher) was labelled with a quantum dot (Qdot 705 ...
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bioRxiv - Immunology 2021Quote: ... VSVg tag-158Gd (rabbit pAb, Thermo Fisher Scientific), E tag-154Sm (clone 10B11 ...
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bioRxiv - Immunology 2021Quote: ... anti-HSV tag (rabbit pAb, Thermo Fisher Scientific), anti-NWSHPQFEK (NWS ...
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bioRxiv - Biochemistry 2020Quote: ... Primary antibodies: mouse monoclonal anti-6xHistidine tag (Invitrogen) and mouse monoclonal anti-GAPDH (ref ...
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bioRxiv - Biophysics 2021Quote: ... anti HA-tag (Invitrogen, 71-5500, 1:250) and DAPI ...
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bioRxiv - Systems Biology 2020Quote: ... labeled with TMT 10plex isobaric tags (Thermo Fisher), the labeled and combined samples were fractionated into 20 fractions each using basic-pH reversed-phase chromatography and analyzed on an Easy-nLC 1200 chromatography system coupled to an Orbitrap Fusion Tribrid mass spectrometer (both Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Respective secondary antibodies had an Alexa488 tag (Invitrogen). Western blot membranes were scanned using a ChemiDoc imaging system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... mouse HA-tag (catalogue no. 26183; Thermo Scientific), rabbit p53 (catalogue no ...
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bioRxiv - Molecular Biology 2022Quote: ... and V5 tag (Invitrogen R960-25, 1:1,000).
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bioRxiv - Microbiology 2022Quote: ... namely mouse anti-HA tag (Thermo Fisher Scientific) 1:500 ...