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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Tandem mass tag (TMT) isobaric labeling (ThermoFisher, CA) was performed at 1:4 ratio by weight of sample for 1 h ...
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bioRxiv - Biophysics 2020Quote: ... 1 ml of C-tag capture resin (ThermoFisher) was added to a cleared and filtered S2 supernatant and incubated for 18 hrs at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-V5 tag (Invitrogen, #R960-25), mouse monoclonal anti-GAPDH (GeneTex ...
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bioRxiv - Microbiology 2020Quote: ... with mouse anti-V5 tag (Invitrogen, R960-25) and mouse anti-MLV p30 antibody (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... a TAG stop codon and Gateway™ (Invitrogen) BP recombination sites ...
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bioRxiv - Immunology 2022Quote: ... anti-V5-tag (R960-25, Thermo Fisher Scientific), anti-puromycin (MABE343 ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse monoclonal anti-V5 tag (ThermoFisher, # R960-25) diluted at 1:2500 ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblotting was performed with anti-Myc Tag (ThermoFisher) and anti-FLAG® M2 antibodies (Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... chicken anti-GFP-tag (1:500, #A10262 Invitrogen), rat anti-CD206 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-V5 tag (Thermo Fisher Scientific, R960-25), anti-IgG(H+L)-Alexa555 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The SUMO tag was removed using Dynabeads (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... monoclonal rabbit anti-GFP-tag antibodies (Invitrogen G10362) were used at a ratio of 1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... The FISH Tag™ DNA Multicolor Kit (ThermoFisher) was used to label digested BAC fragments with Alexa dyes following kit instructions ...
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bioRxiv - Immunology 2021Quote: ... anti-Ollas tag (clone L2, Thermo Fisher Scientific), anti-S tag (clone D2K2V ...
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bioRxiv - Immunology 2021Quote: ... anti-VSVg tag (rabbit pAb, Thermo Fisher Scientific). The following secondary antibodies were used ...
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bioRxiv - Immunology 2021Quote: ... anti-V5 tag-152 Sm (Thermo Fisher Scientific), anti-DYKDDDDK (FLAG ...
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bioRxiv - Immunology 2021Quote: ... anti-V5 tag-152 Sm (Thermo Fisher Scientific), anti-DYKDDDDK (FLAG ...
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bioRxiv - Cell Biology 2023Quote: ... HA Tag Monoclonal Antibody (ThermoFisher, 26183, 1:500), Alpha E-Catenin Polyclonal antibody (Proteintech ...
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bioRxiv - Cell Biology 2023Quote: ... HA Tag Monoclonal Antibody (ThermoFisher, 26183, 1:5000), HRP-labeled Streptavidin (Beyotime ...
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bioRxiv - Molecular Biology 2022Quote: ... Tandem mass tag 11-plex reagents (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Westerns were probed for HA-Tag (Invitrogen; #26183) and Myc (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... V5-Tag 1/1000 (Life Technologies, A190- 119A).
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bioRxiv - Developmental Biology 2023Quote: ... pre-mixed TMTpro tags (8-plex Thermo Fisher Scientific 20 μg/μL in dry acetonitrile stored at -80 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Tandem mass tag (TMT) reagents (Thermo Fisher Scientific) were dissolved in anhydrous acetonitrile (ACN ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-Flag-tag (Thermo Fisher Scientific; MA1-91878), anti-GST (CST ...
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bioRxiv - Cell Biology 2022Quote: Mouse anti-V5 tag monoclonal antibody (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Tandem Mass Tag (TMT lot #TC264166, Thermo Scientific) labeling was performed on 330 µg of purified peptides from each sample in a 1:1.7 (peptide:label ...
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bioRxiv - Cell Biology 2022Quote: ... Tandem mass tag (TMT10plex; #90110, Thermo Fisher Scientific) labelling was carried out according to the manufacturer’s instructions with the following changes ...
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bioRxiv - Developmental Biology 2023Quote: ... a GST antibody (GST Tag Monoclonal Antibody, ThermoFisher) and a FLAG antibody (anti-Flag-HRP ...
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bioRxiv - Immunology 2022Quote: ... and anti-6xHIS tag DyLight680 (HIS.H8, ThermoFisher Scientific). A08 was identified via single cell antibody sequencing of human PBMC B cells and did not bind RBD when expressed as a secreted IgG1 ...
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bioRxiv - Immunology 2024Quote: ... V5 tag (1:5000; ThermoFisher Scientific R960-25), actin (1:5000 ...
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bioRxiv - Physiology 2024Quote: ... and V5 tag (1:100, Thermo Fisher Scientific) to detect V5-tagged ER-TurboID ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-human IL6 Ab concentrations were quantified using the Quant-it protein assay kit (Life Technologies). Ab conjugates were validated on 10% TBE urea denaturing gels and validated on an agarose gel (Supplementary Figure S8) ...
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bioRxiv - Immunology 2022Quote: ... The secreted fusion protein was purified on a CaptureSelect™ human albumin affinity matrix (Life Technologies), and protein eluted by adding 20 mM Tris and 2.0 M MgCl2 ...
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bioRxiv - Genomics 2022Quote: ... human preadipocytes in a 6-well plate were transfected with 6.25ug Cas9 protein (Fisher Scientific, A36498) and 800ng sgRNAs (IDT) ...
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bioRxiv - Microbiology 2023Quote: Human ACE2 fusion proteins were obtained from transient transfection using the Expi293TM system (Thermo Fisher Scientific). All plasmids used in this study were generated by ATUM Bio and transient transfection was carried out following the manufacturer’s protocol for the ExpiFectamineTM 293 transfection kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Cell Biology 2020Quote: ... specimens were incubated with 10 nM purified recombinant POLArISact protein or 2-5 nM Alexa Fluor 488 Phalloidin (AF488-phalloidin, A12379, Thermo Fisher Scientific) and 10 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with 1μg/ml of biotinylated recombinant ACE2 protein for 1 hour at room temperature followed by incubation with HRP-conjugated streptavidin (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...
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bioRxiv - Cell Biology 2023Quote: ... and Alt-R® CRISPR-Cas9 tracrRNA Atto550 labeled, IDT) and recombinant Cas9 protein (Alt-R® S. p. HiFi Cas9 Nuclease V3, IDT) using Lipofectamine™ CRISPRMAX (Invitrogen) and cultured for 48 h in the presence of an HDR enhancer (final concentration 20 μM ...
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bioRxiv - Microbiology 2023Quote: Concentration of recombinant proteins were determined by measuring absorbance at 280 nm using a NanoDrop 1000 and/or NanoDrop One (Thermo Fisher Scientific) considering the extinction coefficient and the molecular weight calculated with the ProtParam tool (http://www.expasy.org/tools/protparam.html).
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bioRxiv - Molecular Biology 2022Quote: ... 0.4 picomol of folded reporter mRNA was mixed with 20 picomol of recombinant protein and 200 picomol of UltraPure BSA (Thermo Fisher # AM2618) in binding buffer (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 28 fmol of folded reporter mRNA was mixed with 0-10 picomol of recombinant protein and 100 picomol of UltraPure BSA (Thermo Fisher # AM2618) on ice for 1 hr ...
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bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... the microtiter plates were washed 5 × with T-PBS and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of IgG antibodies from human ...