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Citations for Thermo Fisher :
1151 - 1200 of 1252 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Purity of eluted gRNA product was determined by formalin gel electrophoresis and gRNA was quantified on a Nanodrop 2000c (ThermoFisher Scientific, Waltham, MA). Following quantification ...
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bioRxiv - Cell Biology 2019Quote: ... 100 µg were loaded onto a 1% agarose gel and ran on Owl™ EasyCast™ B1 Mini Gel Electrophoresis Systems (Thermo Scientific) at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: Protein samples were heated for 5 min at 95 °C and separated by electrophoresis in NuPAGE™ 4-12 % gels (ThermoFisher, Cat# NP0329BOX). RNA samples were heated for 2.5 min at 95 °C and separated by electrophoresis in denaturing 9 % acrylamide (19:1 ...
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bioRxiv - Plant Biology 2021Quote: ... All isolated DNA was assessed for concentration and quality by fluorometry and electrophoresis using a Qubit™ 4 and Qubit™ 1X dsDNA HS Assay Kit (ThermoFisher Scientific) and a TapeStation (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... The gradient fractions were then analyzed by alkaline gel electrophoresis and visualized by SYBR Gold Nucleic Acid Gel Stain (Thermo Fisher, Cat# S11494). The peak fraction(s ...
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bioRxiv - Cancer Biology 2021Quote: ... the input DNA quality was verified using gel electrophoresis and the quantity was measured using the Picogreen assay (Thermo Scientific, Waltham, MA, USA). 2ng of DNA was prepared in 30ul of buffer and used for library construction using the Nextera DNA Flex Library Kit (Illumina ...
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bioRxiv - Immunology 2022Quote: ... and transferred using electrophoresis to nitrocellulose membranes by using the iBlot 2 Gel Transfer Device (Thermo Scientific, iBlot 2 NC Regular Stacks, IB23001, Invitrogen, Walham, MA). Membranes were blocked for 1 h at RT in 5% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... the PEG-tagged samples and the negative controls of the respective stable cell lines were resolved by denaturing gel electrophoresis on 3-8% Tris-acetate gradient protein gels (Thermo Fisher Scientific, EA0378BOX) using Tris-acetate-SDS running buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA quality was evaluated with electrophoresis in a 1% (w/v) agarose gel and DNA concentrations were evaluated using Qubit (Thermo Fisher Scientific, USA). An equal amount of DNA from each sample was used for library preparation with the NEBNext DNA Ultra2 Library Preparation Kit (New England Biolabs) ...
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bioRxiv - Plant Biology 2021Quote: ... DNA quality was checked by agarose gel electrophoresis and quantified with a NanoDrop spectrophotometer ND-1000 version (Thermo Fisher Scientific, Wilmington, DE, USA). For GBS library construction ...
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bioRxiv - Microbiology 2020Quote: ... DNA concentration and purity were determined by agarose gel electrophoresis and fluorometrically using RiboGreen (Qubit Assay Kit, Invitrogen, Life Technologies Corporation, Oregon, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The quality and concentration of DNA were determined by 1.0% agarose gel electrophoresis and a Nano Drop® ND-2000 spectrophotometer (Thermo Scientific Inc., USA) and kept at −80 ℃ prior to further use ...
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bioRxiv - Genomics 2019Quote: ... The size of the sequencing library was estimated by capillary electrophoresis using a Fragment Analyzer (AATI, Ames IA) and Qubit quantification (Life Technologies, Carlsbad CA). Cluster generation and paired-end sequencing on Illumina HiSeq instruments were performed according to the manufacturer’s protocols (Illumina ...
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bioRxiv - Plant Biology 2019Quote: ... All PCR-amplified fragments were analyzed by taking 10 μL of reaction and run on an electrophoresis gel of 1.5% agarose (Life Technologies, Carlsbad, CA, USA) and 1x TBE (50 mM Tris-HCl pH 8 ...
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bioRxiv - Zoology 2020Quote: ... Fish were assigned back to families via microsatellite genotyping using the SPAN B suite of loci (37) on an ABI 3730 capillary electrophoresis system with GeneMapper version 4.1 to call peaks (Applied Biosystems, Foster City, California). The SOLOMON program (38 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and visually evaluated by gel electrophoresis on a 1.2% agarose gel (GelRed nucleic acid stain, Lambda DNA/HindIII Marker and 1kb DNA marker (Invitrogen; Thermo Fisher Scientific, Germany)).
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bioRxiv - Pathology 2021Quote: Amplicons were purified by agarose gel electrophoresis and sequenced directly using the BigDye Terminator v3.1 Cycle Sequencing Kit on an ABI Prism R3500 (Applied Biosystems, Foster City, CA.). Twenty six previously published sequences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific, Dreieich, Germany). The following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Cell Biology 2021Quote: ... and the soluble fractions were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on 6% Tris-Glycine gels (Life Technologies, Carlsbad, CA)64 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and analyzed by SDS-PAGE (SDS-polyacrylamide gel electrophoresis) using Novex™ 14% Tris-Glycine protein gels (Cat. #XP00145BOX, Thermo Scientific, MA, USA). Protein bands were visualized with Coomassie Brilliant Blue staining.
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bioRxiv - Cell Biology 2021Quote: ... 10µg of protein was submitted to SDS-PAGE electrophoresis using NuPAGE Novex or Bolt 4-10% Bis-Tris gels (Thermo Fisher Scientific, USA). Proteins were transferred to nitrocellulose membranes using the iBlot Dry Blotting System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and then stacked in a single band in the top of a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel (4%–12% NuPAGE gel, Invitrogen, Illkirch-Grafenstaden/France). After staining with R-250 Coomassie Blue (Biorad ...
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bioRxiv - Genomics 2021Quote: ... Qualitative and quantitative RNA assessment of all samples was done by gel electrophoresis (1% agarose), a NanoPhotometer® P330 (Implen, Munich/Germany) and a Qubit 2.0 (Invitrogen, Carlsbad, CA/USA).
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bioRxiv - Neuroscience 2022Quote: ... The purity of produced viruses was routinely checked by silver staining (Pierce, Germany) after gel electrophoresis (Novex™ 4–12% Tris–Glycine, Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2022Quote: ... Electrophoresis was conducted at 150 V for 4 h on ice in NuPAGE™ MES SDS Running Buffer (20X) (Thermo Fisher Scientific, NP0002) supplied with 1x NuPAGE™ Antioxidant (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... of nuclear or 40 μg of cytosolic proteins were resolved by SDS-polyacrylamide gel electrophoresis in a 12% polyacrylamide gel (NuPAGE Gel, NP0335BOX; Thermo Fisher Scientific, USA). Proteins were transferred to nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNA concentration and purity were monitored by agarose electrophoresis and determined by UV absorption analysis using NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, USA). The V4 hypervariable region of the 16S rRNA bacterial gene (515-806 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were heated to 95 °C for 5 min prior to SDS-PAGE gel electrophoresis (NuPAGE MES SDS running buffer, Thermo Fisher Scientific, #NP0002). Proteins were transferred to a nitrocellulose membrane (#1704158 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2019 and from callus tissues using RNeasy Plant Mini Kit (Qiagen).The quality and concentration of extracted RNA were checked with 1 % agarose gel electrophoresis and estimated with Multiskan Sky Microplate Spectrophotometer (Thermo Fisher Scientific, USA), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore; Thermo Fisher Scientific). PVDF membrane was treated with primary and secondary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified DNA fragments were resolved by electrophoresis in 1% agarose gels alongside GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific, cat# SM1331). CRISPR/Cas9-induced mutations in AS-NMD-regulated genes were characterized with primers designed to amplify either short (∼0.5 kb ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.005 % BPB) and incubated 5 min at 100 ºC followed by electrophoresis on Novex™ 4-20% Tris-Glycine gradient gels (Invitrogen, WXP42020BOX; WXP42026BOX). Proteins were transferred onto 0.2 µm nitrocellulose membranes (Pall Laboratory ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins in the lysates were separated by gel electrophoresis with Bolt™ 4-12% Bis-Tris Plus Gels (Invitrogen™, CAT: NW04122BOX) and transferred to Immobilon-FL PVDF Membranes (MilliporeSigma™ ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA quality and concentration were assessed by electrophoresis on a 2% agarose gel and a Qubit 4.0 Fluorometer (Thermo Scientific, Waltham, MA, USA). DNA sequencing libraries were prepared following standard MGI sequencing library protocol by the Beijing Genomics Institute (BGI ...
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bioRxiv - Synthetic Biology 2023Quote: ... the amplicons were separated in a 1% agarose gel after electrophoresis at 140 V and identified by size based on gene ruler Plus DNA Ladder (Thermo Fisher Scientific, Lithuania). Amplicons were purified using Favorprep™ GEL/PCR Purification Kit (Favorgen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 5 μl of product from 35 rounds of PCR were analyzed for each time-point in by 2% agarose gel electrophoresis (Top Vision, Thermo Scientific, Waltham, MA). Each result is representative of a minimum of two independent replicates.
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bioRxiv - Genetics 2023Quote: 10µL of the PCR product was used to visualise amplification via gel electrophoresis on a 1% Agarose gel containing SYBR™ Green I Nucleic Acid Gel Stain (Thermo Fisher Scientific). The rest of the PCR product was cleaned using Monarch PCR & DNA cleanup kit (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 µg of total protein fraction for each sample was separated by electrophoresis using NuPAGE™ 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific). Proteins were transferred to a polyvinylidene difluoride membrane using an XCell SureLock Mini-Cell Electrophoresis System (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... Purity of produced viral vectors was routinely checked by silver staining (Pierce, Germany) after gel electrophoresis (Novex™ 4–12% Tris–Glycine, Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... 20μg of protein was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on NuPAGE 4-12% Bis-Tris gels (Thermo Fisher Scientific #NP0329). The gel was blotted onto Immobilon-FL PVDF Membrane (Millipore #IPFL00010 ...
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bioRxiv - Plant Biology 2023Quote: ... RNA quality was assessed by electrophoresis in agarose gel and quantified using a QubitTM RNA Broad Range kit and Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA).
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bioRxiv - Neuroscience 2024Quote: ... A total of 30 μg of protein fraction for each sample was separated by electrophoresis using NuPAGETM 4-12% Bis-Tris Protein Gel (ThermoFisher Scientific, Waltham, MA). Proteins were transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2024Quote: ... NuPage® 4-12% Bis-Tris Gels were loaded with sample and run in NuPage® SDS MOPS Running Buffer using the XCell SureLock™ Mini-Cell Electrophoresis System (all Life Technologies) for 50 min at 200 V ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysates (20 μg) were analyzed via electrophoresis using 4%-12% sodium dodecyl sulphate-polyacrylamide gradient gels (Thermo Fisher Scientific, Waltham, MA, USA). Pre-stained protein ladders (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... for each sample were loaded onto 10% polyacrylamide gels and separated by electrophoresis and transferred overnight to nitrocellulose membranes (Thermo Fisher, Stockholm, Sweden). The membranes were immunoblotted with primary antibodies against actin (1:30,000 ...
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bioRxiv - Immunology 2021Quote: ... Antibody purity and proper size was verified by Coomassie stained sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Evolutionary Biology 2021Quote: ... The amplicons were separated using the agarose gel-electrophoresis and cloned using TOPO TA Cloning Kit for Sequencing (Thermo Fisher Scientific K.K., Tokyo, Japan) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... One μg of total protein per sample was resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% bis-tris gels in MOPS SDS running buffer (NuPAGE® Novex® SDS-PAGE system; Thermo Fisher Scientific) and transferred to polyvinylidene fluoride membranes (iBlot® 2 gel transfer system ...
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bioRxiv - Genomics 2020Quote: ... The DNA quality and concentration were assessed using electrophoresis on 1.0% agarose gels and an ND-2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). The genomic DNA was used for Illumina and ONT library construction and then sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... final expression clones were validated by agarose gel electrophoresis and restriction digest and were used to generate bacmid DNA with the Bac-to-Bac system (Thermo Fisher Scientific, Waltham, MA) using the manufacturer’s instructions.