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Citations for Thermo Fisher :
801 - 850 of 1252 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The resulting samples were separated by electrophoresis on 2.5% agarose gel and signals were detected using SYBR™ Gold Nucleic Gel Stain (Invitrogen).
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bioRxiv - Immunology 2020Quote: ... Samples failing any quality control step (DNA quality assessed by capillary electrophoresis (Fragment analyzer, Advance analytical) and quantity (Picogreen quantification assay, Thermofisher) were eliminated from further downstream steps19 ...
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bioRxiv - Genetics 2020Quote: ... and denatured for 5 minutes at 94°C prior to capillary electrophoresis (Applied Biosystems 3500 Genetic Analyzer, Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... Fourteen μL of clarified lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 10% polyacrylamide Bis-Tris gels (Invitrogen) and transferred onto Immobilon FL PVDF membranes (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... the supernatants were processed for loading to Bis-Tris 4-12% gradient Bolt PAGs using Bolt loading dye solution and reducing agents and electrophoresis in MES running buffer (all from Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The purified protein was analyzed by SDS Page electrophoresis and western blot analysis using anti-His antibodies as per manufacturer guidelines (ThermoFisher).
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bioRxiv - Genetics 2022Quote: ... Protein concentration was determined with the BCA protein assay kit (Pierce) and 75 μg of protein was resolved by electrophoresis using 12% Bis-Tris Plus NuPAGE gels (Invitrogen) and transferred to PVDF membranes (iBlot 2 system (Invitrogen)) ...
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bioRxiv - Zoology 2022Quote: ... PCR products were visualized using 1.2% agarose gel electrophoresis and purified using the ExoSAP procedure (Exonuclease I - Shrimp Alkaline Phosphatase from ThermoFisher, USA). PCR products were sequenced bi-directionally on an ABI automated capillary sequencer (ABI3130xl ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were then purified by agarose gel electrophoresis and subcloned into a pCR2.1 vector with the TA Cloning kit (Life Technologies) according to the supplier’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were verified using standard TAE-agarose gel (1.5%) electrophoresis with the SYBR® Green I nucleic acid gel stain (Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... SDS-PAGE was carried out by resolving the samples by electrophoresis in NuPAGE 4-12% Bis-Tris Protein polyacrylamide gels (#NP0323BOX, Invitrogen). After the electrophoresis ...
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bioRxiv - Genomics 2022Quote: ... The quality of genomic DNA and RNA was evaluated using agarose gel electrophoresis and Qubit 3.0 Fluorometer (Life Technologies, USA).
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bioRxiv - Plant Biology 2023Quote: ... 1 μl Coomassie Brilliant Blue G-250 and 2 μl water were added according to the manufacturer’s instructions (NativePageTM Bis-Tris Mini gel electrophoresis protocol, Thermo-Fisher Scientific). Samples were loaded on a NativePAGE™ 3-12% Bis-Tris Protein Gel (Thermo-Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... Proteins were separated by electrophoresis in NuPAGE™ 4 to 12% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific, USA), and then transferred to Immobilon®-FL PVDF Membrane (Millipore ...
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bioRxiv - Physiology 2022Quote: ... Tissue proteins (15 µg) were separated using electrophoresis on a NuPAGE 4-12% Bis-Tris gel (Invitrogen, Carlsbad, CA, USA) and transferred to a PVDF membrane ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were separated by electrophoresis using a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and transferred to a Hybond-P PVDF membrane (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were separated by electrophoresis using a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and transferred to a Hybond-P PVDF membrane (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The protein and its purity were checked at each step of the purification by SDS-PAGE electrophoresis (4-12% Bis-Tris NuPAGE, MES as running buffer, Invitrogen) stained with SimplyBlue SafeStain (Novex ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total DNA quality was checked using gel electrophoresis and yield was quantified using a Qubit DNA Assay Kit (Thermo Fisher). Families were pooled in equimolar concentrations such that each individual was represented equally to generate 4 libraries ...
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bioRxiv - Neuroscience 2023Quote: ... 30 μg protein/sample was resolved on NuPAGE 4–12% Bis-Tris gels on the NuPAGE electrophoresis system (Life technologies). Proteins were then transferred onto PVDF membranes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quality of extractions were checked with agarose gel electrophoresis and the quantity of extractions (20ng/µl) using a Qubit 3 Fluorometric Quantitation Kit (Invitrogen). We sequenced 94 C ...
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bioRxiv - Biochemistry 2023Quote: ... Protein material was separated via SDS– polyacrylamide gel electrophoresis (SDS-PAGE) (12, 15, or 17 well gels, 4-20% Bis-tris BOLT gels, Invitrogen), with 1X MOPS Buffer (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: Protein constructs were subjected to native electrophoresis on NativePAGE™ Novex® Bis-Tris Gel system following manufacturer’s instructions (ThermoFisher). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... tRNAs were ligated to a Cy3-labelled fluorescent stem-loop RNA/DNA oligonucleotide and subjected to gel electrophoresis using a 10% TBE-UREA denaturing-polyacrylamide gel (Invitrogen). tRNA was visualized using a Licor Odyssey imaging system.
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bioRxiv - Biochemistry 2023Quote: ... and analyzed by agarose gel electrophoresis followed by staining with SYBR Gold nucleic acid gel stain (Thermo Fisher Scientific, #S11494). Fluorescent signals were collected using the Amersham Typhoon scanner 5 system (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... Integrity of extracted genomic DNA was assessed via agarose gel electrophoresis and DNA concentration quantified fluorometrically using the Qubit (Invitrogen) according to manufactures instructions.
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bioRxiv - Neuroscience 2023Quote: ... Electrophoresis was carried out for 25 minutes at 200V before blotting onto nitrocellulose membranes using the iBlot system (ThermoFisher Scientific). Membranes were blocked for a minimum of 30 minutes at room temperature in 5% milk in PBS-T ...
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bioRxiv - Immunology 2023Quote: ... The PCR products were subjected to agarose gel electrophoresis and visualized by staining with SYBR Safe DNA Gel stain (Invitrogen).
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bioRxiv - Physiology 2023Quote: ... Twenty µg of protein in each sample was resolved by electrophoresis using 4-12% Bis-Tris gels (Thermo Fisher Scientific) and transferred to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... DNA quality was assessed by agarose gel electrophoresis and quantified using a QuBit 3 Fluorometer (Thermo-Fisher Scientific, Renfrew, Scotland). Amplicon sequencing was then carried out using the universal bacterial and archaeal primer set (515f and 806r ...
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bioRxiv - Cancer Biology 2023Quote: ... Sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blotting were performed using pre-cast NuPAGE Bis-Tris 4 - 12% mini-gels (Invitrogen) with 1X MOPS buffer (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... and quality was assessed by electrophoresis with 2% Agarose EX-gels using the E-Gel iBase (Invitrogen, Grand Island, NY). Fluidigm Access Array was used to generate 16S rRNA gene amplicons ...
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bioRxiv - Genomics 2022Quote: ... The quality of extracted DNA was visualized using 1% agarose gel electrophoresis and quantified using Quant-iT PicoGreen ds-DNA Assay kits (Thermofisher) and a fluorescent plate reader ...
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bioRxiv - Genomics 2023Quote: ... The quality and quantity of fragmented DNA were verified using agarose gel (1.5%) electrophoresis and Qubit® 4.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... denatured by heating for 5 min at 95°C and subjected to electrophoresis using precast NuPAGE Novex 4–12% Bis-Tris Proteins Gels (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled for 5 min and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on either 10% gels (cast freshly) or 4-20% gradient gels (Invitrogen). Proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples (20 ug) were used to perform electrophoresis in 1x Bolt™ MOPS SDS Running Buffer (Fisher Scientific, B0001) in Tris-buffered saline containing Tween-20 (TBST ...
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bioRxiv - Genetics 2023Quote: ... PCR amplification products were separated and detected by capillary gel electrophoresis on an ABI 3730 Genetic Analyzer (Applied Biosystems Inc.) including a size standard GeneScanTM-500 ROX (Applied Biosystems Inc.) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by electrophoresis at 16 mA followed by transferring the proteins to Immobilon-P membranes (Thermo Fisher Scientific) for 1 hour at 350 mA (4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Beads were pelleted and the entire volume of supernatant was used for western blotting along with input lysate using the NuPAGE electrophoresis and transfer system (Invitrogen) and near-infrared detection for HDAC6 (CST #7558 ...
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bioRxiv - Microbiology 2023Quote: ... Equal concentrations of protein were prepared under reducing conditions and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (4–20% Tris Glycine 1.0mm Mini Protein Gels from Invitrogen XP04200BOX). Gels were transferred to PVDF membranes using the iBlot™ 2 transfer stacks (ThermoFisher IB24002) ...
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bioRxiv - Cell Biology 2023Quote: ... Flagellar suspensions obtained through isolation following the described protocol were loaded in ready-to-use 4-12% electrophoresis gels (NuPAGE Bis-Tris Mini, Invitrogen), together with a molecular weight ladder (PAGERuler Plus or Chameleon Duo) ...
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bioRxiv - Cell Biology 2023Quote: Collected cell pellets were lysed in RIPA and separated by electrophoresis on 4-12% NuPAGE Bis-Tris Mini Protein gels (Invitrogen), followed by semi-dry transfer to nitrocellulose membranes using a Power Blotter apparatus (Invitrogen) ...
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bioRxiv - Physiology 2023Quote: ... Liver proteins (15 μg) were separated via electrophoresis on a NuPAGE 4-12% Bis-Tris gel (Invitrogen, Carlsbad, CA, USA) and subsequently transferred to a PVDF membrane ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µg of each sample were subjected to Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS/PAGE) using precast 10% Bis-Tris NuPage Gels (Invitrogen), transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... the reaction mix was cooled to 4°C and analyzed by standard agarose gel electrophoresis with 2% (w/v) Agarose in TAE Buffer with 1:20.000 SybrSafe (Thermo Fisher), followed by imaging with Odyssey Fc at 600nm.
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bioRxiv - Physiology 2023Quote: ... standard glycine-based gel electrophoresis was applied with polyvinylidene difluoride (PVDF) and the following antibodies: SERCA1 (MA3-911; ThermoFisher Scientific), SERCA2 (MA3-919 ...
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bioRxiv - Developmental Biology 2023Quote: Protein extracts were separated by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (Invitrogen). Blocking and antibody incubations were performed in 5% bovine serum albumin ...
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bioRxiv - Genomics 2023Quote: ... DNA quality was assessed with agarose gel electrophoresis (1% TBE gel) and extracts were quantified with Qubit v2.0 (Life Technologies). Double-digest restriction site-associated DNA (ddRAD ...
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bioRxiv - Microbiology 2023Quote: ... The digestion of DNA was determined by agarose gel electrophoresis and visualized by SYBR Safe DNA gel stain (Invitrogen, USA). The reaction samples of V2c or its variants were scaled up for Western blotting analysis ...