Labshake search
Citations for Thermo Fisher :
901 - 950 of 1252 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Supernatants from overnight bacterial cultures were boiled in LDS sample buffer and separated by electrophoresis using NuPAGE 12% polyacrylamide gels (Life Technologies). The contents of the gels were transferred to PVDF membranes ...
-
Genomic and phenotypic characterization of finger millet indicates a complex diversification historybioRxiv - Genetics 2021Quote: ... The purity and quantity of extracted gDNA was determined by gel electrophoresis and a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA), respectively ...
-
bioRxiv - Genetics 2020Quote: ... DNA samples were genotyped for 46 Ancestry Informative Markers (AIM)-Indels in a multiplex PCR system followed by capillary electrophoresis in an ABI 3500 Genetic Analyzer (Thermo Fisher), as described previously [51,52] ...
-
bioRxiv - Microbiology 2021Quote: The plasmid DNA yields from the sewage samples were evaluated using gel electrophoresis and Qubit double-stranded DNA (dsDNA) BR assay kit on a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA). Plasmid DNA purity was measured and validated by absorbance ratio of 260/280 and 260/230 using NanoDrop 100 (ThermoFisher) ...
-
bioRxiv - Neuroscience 2020Quote: ... The amplified products were resolved by gel electrophoresis in 2% Roti®agarose (Agarose High Resolution; Roth) in 0.5x UltraPureTM TRIS-Borat-EDTA (TBE) buffer (Thermo Fisher). Non-intron spanning primers for DAZL (DAZL(I)) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... PCR products were visualized via agarose gel electrophoresis and nucleic acid staining with SYBR safe DNA stain (S33102, Thermo Fisher Scientific), and imaged using an EZ Gel Documentation System (Bio-Rad ...
-
bioRxiv - Microbiology 2021Quote: Proteins extracted from cell lysates were resolved by SDS-polyacrylamide gel electrophoresis on 4-12% NuPAGE Bis-Tris Gel (Life Technologies) with MOPS running buffer and semi-dry transferred to a nitrocellulose membrane with Trans-Blot Turbo system (Bio-Rad) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... PCR product visualization was performed using agarose gel electrophoresis with appropriate agarose concentration and SYBR® Safe DNA Gel Stain (Invitrogen).
-
bioRxiv - Microbiology 2021Quote: ... All in vitro transcribed RNAs were analysed by electrophoresis in 1.5% formaldehyde agarose gel and RNA concentration was determined on Nanodrop ND-1000 spectrophotometer (ThermoFisher Scientific, USA). RNA was transfected into cells using in-suspension protocol53.
-
bioRxiv - Molecular Biology 2020Quote: ... The PCR amplicons were confirmed on a 1% agarose gel electrophoresis and purified with ExoSAP-IT™ PCR Product Cleanup Reagent (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: Viral and cell lysates were resuspended in SDS sample buffer and separated by electrophoresis on Bolt 4-12% Bis-Tris Plus gels (Life Technologies), blotted onto nitrocellulose membranes ...
-
bioRxiv - Molecular Biology 2021Quote: ... After protease exposure the samples were analysed by reducing and denaturing polyacrylamide gel electrophoresis on 4-12% bis-tris gels (Life Technologies). Protein bands were developed with Simply Blue Safe Stain (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... Extracted DNA was assessed for quality by electrophoresis and was quantified using a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). DNA isolated from each sample was amplified using the universal primers ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were then separated using gel electrophoresis using 4-12% Bis-Tris Novex Midi 20 well gels (Invitrogen, Cat. No. #WG1402BOX) with 1X MES Buffer (Invitrogen ...
-
bioRxiv - Cancer Biology 2021Quote: ... equal amounts of protein (typically 20-50 µg of protein per lane) were separated by electrophoresis on a NuPAGE 10% Bis-Tris Gel (Invitrogen, NP0315BOX) in NuPAGE™ MOPS SDS Running Buffer (20X ...
-
bioRxiv - Cell Biology 2020Quote: ... Identity and purity of CIZ1-containing elution fractions was assessed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) with SimplyBlue™ safe stain (Invitrogen) and pre-stained protein ladder 10-250 kDa (Thermo Scientific) ...
-
bioRxiv - Biochemistry 2022Quote: ... The mixtures were then analyzed by electrophoresis through a urea-15% polyacrylamide gel (Novex TBE-Urea gel 15%, Thermo Fisher Scientific). To acquire the fluorescent signals ...
-
bioRxiv - Molecular Biology 2022Quote: ... and purity parameters were evaluated for RNA extractions by capillary electrophoresis (Fragment Analyzer, STANDARD SENSITIVITY RNA ANALYSIS KIT DNF-471, Advanced Analytical Technologies) and spectrophotometry (Nanodrop2000, Thermo Scientific), procuring to have more than two micrograms of RNA ...
-
bioRxiv - Physiology 2022Quote: ... Products were then analysed by 1% agarose gel electrophoresis in TBE buffer followed by SYBR Gold staining (catalog no. S11494, ThermoFisher Scientific). Gels were imaged with a ChemiDoc MP Imaging System ...
-
bioRxiv - Microbiology 2022Quote: ... The quality and quantity of the retrieved DNA were assessed both before and after treatment via gel electrophoresis and Nanodrop analysis (ThermoFisher Scientific). Sequencing libraries for salivary samples were prepared using the NEBNext Ultra DNA Library Prep kit (New England Biolabs ...
-
bioRxiv - Biophysics 2022Quote: ... Electrophoresis was performed for 60 minutes at 150 V using 0.5 x TBE and stained with SYBRsafe (Thermo Fisher, MA, USA) according to the manufacturer’s specifications for 30 minutes before imaging using UV transillumination.
-
bioRxiv - Cell Biology 2022Quote: Protein lysates of HAP1 and HeLa cells were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% gradient precast polyacrylamide gels (Life Technologies). The proteins separated by SDS-PAGE were transferred onto a nitrocellulose membrane using a Mini-Protean® Tetra Cell device ...
-
bioRxiv - Cell Biology 2022Quote: ... The quality and quantity of DNA isolation across the 96 well plate were measured using agarose gel electrophoresis and Nanodrop (ThermoFisher Scientific), respectively ...
-
bioRxiv - Cancer Biology 2022Quote: ... cell lysates were mixed with 6X sample buffer containing β-ME and were separated by SDS-PAGE electrophoresis using NuPAGE 4-12% Bis-Tris gels (Invitrogen), and transferred onto the 0.45 μm nitrocellulose membranes ...
-
bioRxiv - Neuroscience 2021Quote: ... The designated samples were then subjected to one-dimensional gel electrophoresis (1DE) employing precast NuPAGE 4-12% Bis-Tris 10-well mini protein gels (Invitrogen, Germany) with 2-[N-morpholino]ethanesulfonic acid (MOPS ...
-
bioRxiv - Microbiology 2020Quote: ... and samples were heated to 95°C for 5 minutes and subjected to electrophoresis in 12% NuPAGE Bis-Tris gels (Life Technologies). Gels were transferred to nitrocellulose membranes (Life Technologies ...
-
bioRxiv - Microbiology 2019Quote: Viral and cell lysates were resuspended in sodium dodecyl sulfate (SDS) sample buffer and separated by electrophoresis on Bolt 4-12% Bis-Tris Plus gels (Life Technologies), blotted onto nitrocellulose membranes and probed overnight at 4°C with the following antibodies in Odyssey Blocking Buffer (LI-COR) ...
-
bioRxiv - Microbiology 2021Quote: ... and was then analyzed by electrophoresis on a 1% agarose gel as well as a Qubit 2.0 (Thermo Scientific, Waltham, USA). DNA libraries were generated using a NEBNext UltraTM II DNA Library Prep Kit (NEB ...
-
bioRxiv - Neuroscience 2021Quote: ... Equal amounts of protein (20-50 μg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (ThermoFisher Scientific), transferred to polyvinylidene difluoride membranes ...
-
bioRxiv - Microbiology 2020Quote: Protein samples were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using NUPAGE 10% Bis-Tris Gels (Life Technologies), run in NUPAGE MES Running Buffer (Life Technologies) ...
-
bioRxiv - Microbiology 2021Quote: ... The resulting amplification products were verified by gel electrophoresis and cleaning and normalization of individual PCR products was performed with SequalPrep™ Normalization Plate (96) Kit (Invitrogen). The normalized PCR amplicons were mixed ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were then separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using NuPAGE Bis-Tris gels (Thermo Fisher Scientic) and transferred on nitrocellulose membranes (iBlot 2® gel transfer Stacks Nitrocellulose system from Invitrogen) ...
-
bioRxiv - Cancer Biology 2022Quote: ... of SEC-purified small EVs and cell lysates were separated by SDS-polyacrylamide gel electrophoresis (PAGE; NuPAGE 4-12% Bis-Tris Protein Gel, ThermoFisher Scientific) and transferred to PVDF membranes (Millipore ...
-
bioRxiv - Molecular Biology 2022Quote: ... were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) before transferring onto 0.45 μm nitrocellulose membrane (Thermo Scientific™) with a Hoefer TE77X semi-dry transfer unit at 20V for 1 h ...
-
bioRxiv - Pathology 2022Quote: ... sites flanking the donor DNA sequence and the linearized donor DNA was purified by agarose gel electrophoresis with GeneJet Gel Extraction kit (Thermo Fisher). Linearized donor DNA and CRISPR/Cas9 complex were injected into C57BL/6 fertilized zygotes ...
-
bioRxiv - Biophysics 2019Quote: ... Single strands were separated by electrophoresis in a 1.2% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific). After electrophoresis ...
-
bioRxiv - Zoology 2019Quote: PCR products were run and purified by agarose gel electrophoresis and cloned into the pJET 1.2 cloning vector (Thermo Fisher Scientific). The inserts were sequenced.
-
bioRxiv - Microbiology 2019Quote: ... Reaction products were separated by agarose gel electrophoresis and the amplicon derived from the extended array was purified from the gel using a GeneJET Extraction Kit (ThermoFisher Scientifc) and sequenced on a NextSeq 500 system.
-
bioRxiv - Microbiology 2019Quote: ... Final cDNA libraries with ligated adaptors were size-selected to fall between 250 and 500 bp using the E-Gel® electrophoresis system (Invitrogen). cDNA quantity was measured using the Quant-iT™ PicoGreen® dsDNA Assay Kit (Thermo Fisher ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... PCR products were visualized by agarose gel electrophoresis and nucleic acid staining with SYBR safe DNA stain (S33102, Thermo Fisher Scientific), and imaged with an EZ Gel Documentation System (Bio-Rad ...
-
bioRxiv - Microbiology 2019Quote: ... All samples were checked for quality and quantity with gel electrophoresis and fluorescence-based analysis (Qubit 2.0, Thermo Fisher Scientific, USA) before being used in a second PCR step with barcoded B341 forward primer and reverse B785 primer containing the P1 adapter ...
-
bioRxiv - Developmental Biology 2019Quote: ... we eluted proteins and analyzed samples by electrophoresis on 4%–12% NuPAGE SDS-PAGE gels with MOPS SDS Running buffer (Life Technologies) and corresponding LDS buffer NuPage (Invitrogen ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Each individual reaction (containing PCR products for all 5 loci) was resolved by electrophoresis on an ABI 3100 automated sequencer (Applied Biosystems). Under these thermal conditions ...
-
bioRxiv - Molecular Biology 2020Quote: ... ref.17-1318-02) before being subjected to SDS-PAGE gel electrophoresis (NuPage™ 4-12% Bis-Tris Gel Invitrogen, NP0322box) and semi-dry transfer to a nitrocellulose membrane (iBlot®2NC Mini Stacks Invitrogen ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were analyzed by 20 % denaturing polyacrylamide gel electrophoresis as above and stained with SYBR Gold (Thermo Fisher Scientific, Waltham, MA).
-
bioRxiv - Molecular Biology 2020Quote: ... after denaturation by 70°C for 3 minutes and separated by gel electrophoresis using 10% TBE-Urea gels (Invitrogen, Cat# EC68755BOX) at 180V for 60 min in 1x TBE electrophoresis buffer (Bio-Rad ...
-
bioRxiv - Plant Biology 2021Quote: ... and the quality and quantity of the sample was assessed using agarose gel electrophoresis and a fluorometric procedure (Qubit Fluorometer, Invitrogen, USA) by the Beijing Genomics Institute (BGI ...
-
bioRxiv - Pathology 2021Quote: ... The PCR amplicon with an attB sequence at the end was visualized using gel electrophoresis and purified using the GeneJet Gel Extraction Kit (Thermo Scientific). Fragments were cloned into the pDONOR vector via a BP Clonase reaction (Invitrogen) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... PCR amplicons were size-verified by electrophoresis on a 0.8% TAE agarose gel stained with SYBR Safe (Thermo Scientific, Waltham, MA) and then excised and purified using the Wizard® SV Gel and PCR Clean-Up System kit (Promega ...
-
bioRxiv - Immunology 2020Quote: ... 75 μg of total proteins were separated by 15 % SDS–polyacrylamide gel electrophoresis and afterwards transferred onto polyvinylidene difluoride membranes using a transblot transfer chamber (X-Cell SureLock™, Invitrogen Novex Mini-cell ...