Labshake search
Citations for Thermo Fisher :
951 - 1000 of 1252 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
-
bioRxiv - Microbiology 2020Quote: ... PCR products were analyzed by 0.8% or 2% agarose gel electrophoresis and visualized under UV after staining with SYBR safe DNA gel stain (Invitrogen, Carlsbad, California). PCR products were purified with the Zymoclean Gel DNA Recovery Kit (Zymo Research ...
-
bioRxiv - Microbiology 2020Quote: ... The quality and quantity of the DNA extraction were evaluated by 1% agarose gel electrophoresis and Qubit 3.10 Fluorometer (Invitrogen, Life, USA). The good-quality DNA was first sheared randomly to fragments of around 500bp by Focused-ultrasonicattor (Covaris M220 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Cells were lysed in Laemmli buffer and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane (ThermoFisher, 88518). Membranes were blocked with 5% milk in TBST for 1h ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... DNA quality and quantity were assessed using agarose gel electrophoresis and the Qubit Fluorometer with the dsDNA BR Assay (Life Technologies). ddRADseq libraries were prepared using a modified version of the Recknagel et al ...
-
bioRxiv - Microbiology 2019Quote: ... After extraction the DNA was checked for quality by gel electrophoresis and quantified using a Qubit 4 (Thermo Fisher Scientific, U.S.A.).
-
bioRxiv - Systems Biology 2019Quote: ... Equivalent amounts of protein in the specimen were separated based on their molecular weight by electrophoresis on 8% Bis-Tris gels and transferred to polyvinylidene difluoride (PVDF) membranes (Life Technologies). Membranes were immediately blocked with 5% nonfat dried milk diluted in TBST and incubated with primary antibodies (1:1,000 ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... DNA concentration was evaluated through agarose gel electrophoresis and fluorometry using a Qubit 2.0 (Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA). Library preparation ...
-
bioRxiv - Microbiology 2021Quote: ... Triplicate reactions were pooled after PCR and amplified DNA was visualized by agarose gel electrophoresis and quantified by Pico green (Thermo Fisher). After cleaning with Ampure beads (Beckman Coulter) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... RNA concentration was measured through electrophoresis by loading 1μl of each sample on 1% agarose gel and by spectrophotometry through Nanodrop 3300 (Thermo Fisher Scientific).
-
bioRxiv - Neuroscience 2021Quote: ... Equal amounts of protein (20-50 μg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (ThermoFisher Scientific), transferred to polyvinylidene difluoride membranes ...
-
bioRxiv - Molecular Biology 2022Quote: ... Capillary electrophoresis and automated base calling was performed in ABI 3130 Genetic Analyzer and Sequencing analysis software V5.2 (Applied Biosystems, USA) according to manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... and the sequencing reactions were separated with capillary electrophoresis (POP7 polymer; ABI Prism 3130xl apparatus; Applied Biosystems, Foster City, CA, USA) according to the standard manufacturer’s recommendations.
-
bioRxiv - Developmental Biology 2021Quote: ... RNA integrity was determined by agarose electrophoresis and concentration measured using Qubit RNA Assay Kit in a Qubit 2.0 Fluorometer (Life Technologies, Invitrogen). cDNA libraries were synthetized from 1 µg of total RNA and sequenced using a HiSeq-3000 platform (Illumina ...
-
bioRxiv - Synthetic Biology 2021Quote: ... All assembled plasmids (pFB66 to pFB72, pFB96 to pFB130) were checked correctly by agarose gel electrophoresis after FastDigest XbaI (Thermo Scientific) digestion ...
-
bioRxiv - Cell Biology 2020Quote: ... The presence of frameshift-causing insertion/deletions in both alleles of the obtained clones was achieved by capillary electrophoresis on a 3730xl DNA analyser (Applied Biosystems) and amplifying the targeted region by PCR using a gene-specific 5′ 6-carboxyfluorescein (FAM)-labelled oligonucleotides (Hjelm et al. ...
-
bioRxiv - Plant Biology 2021Quote: ... DNA integrity was assessed using agarose gel electrophoresis and quantified with a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a Bio-Tek Powerwave HT microplate reader ...
-
bioRxiv - Microbiology 2021Quote: ... the quality of genomic DNA was evaluated by pulsed-field gel electrophoresis (Sage Science Pippin Pulse) and genomic DNA concentration was quantified using a Qubit 3.0 Fluorometer (Life Technologies Q33216). Briefly ...
-
bioRxiv - Microbiology 2021Quote: ... 50 μl fractions were collected and analyzed by SDS–PAGE electrophoresis using 4–12% NuPage Bis-Tris precast gels (Life Technologies). Gels were stained with 0.01% (v/v ...
-
bioRxiv - Developmental Biology 2020Quote: ... For the detection of Gli3 10μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
-
bioRxiv - Cell Biology 2021Quote: ... Subcellular fractions were prepared for electrophoresis by addition of 4x Bolt LDS sample buffer and reducing agent (Invitrogen, Carlsbad, CA, USA) followed by heating at 37°C for 30 min ...
-
bioRxiv - Genetics 2020Quote: ... PCR products were confirmed by agarose gel electrophoresis and sequentially either purified using the PureLink PCR Purification Kit (Thermo Fisher Scientific) or gel-extracted using the QIAquick® Gel Extraction Kit (Qiagen) ...
-
bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
-
bioRxiv - Immunology 2022Quote: ... and alkylated with 10mM iodoacetamide prior to sample fractionation by polyacrylamide gel electrophoresis with Novex NuPAGE Bis-Tris 4–12% gels (Life Technologies). Gels were stained with colloidal Coomassie (Sigma ...
-
bioRxiv - Molecular Biology 2022Quote: ... Lysate was loaded and resolved by SDS-PAGE on a 12% Bis-Acrylamide gel and electrophoresis was carried out at using NuPAGE MES SDS running buffer (ThermoFisher Scientific). The separated proteins were transferred onto a nitrocellulose membrane (BioRad Laboratories ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 10 μg of extracted protein was separated using Novex SDS– polyacrylamide gel electrophoresis reagents and transferred to nitrocellulose membranes (Life Technologies). Membranes were blocked in 5% milk protein and incubated with primary antibodies overnight ...
-
bioRxiv - Bioengineering 2022Quote: ... Products of PCR and restriction digests were purified using agarose gel electrophoresis and the GeneJET gel extraction kit (Thermo Fisher Scientific). DNA sequences were analyzed by DNA sequence service of Fasmac Co ...
-
bioRxiv - Genetics 2022Quote: ... 40 μg of each sample was loaded per well and proteins were separated by SDS polyacrylamide gel electrophoresis using precast NuPAGE gels (Invitrogen, NP0301BOX). Samples were transferred to 0,45 µm PVDF membrane (Thermo Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... using agarose gel electrophoresis (1 % agarose gel in 1x TAE buffer; 100 V, 40 min) against a GeneRuler 1 kb ladder (ThermoFisher Scientific).
-
bioRxiv - Microbiology 2022Quote: ... Phage DNA integrity was checked using agarose gel electrophoresis (1 % agarose gel in 1× TAE buffer; 70 V, 90 min) against a GeneRuler 1 kb ladder (ThermoFisher Scientific).
-
bioRxiv - Microbiology 2022Quote: ... The quality and quantity of DNA was assessed by performing gel electrophoresis and the Qubit dsDNA BR Assay Kit (Thermo Scientific), respectively ...
-
bioRxiv - Microbiology 2022Quote: ... were then separated by electrophoresis with either a 12% (v/v) SDS-acrylamide gel or a 4-12% gradient gel (Thermofisher Scientific).
-
bioRxiv - Neuroscience 2023Quote: ... were subjected to SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes or nitrocellulose membranes and blocked with I-Block (ThermoFisher, T2015). Primary antibodies used were ...
-
bioRxiv - Microbiology 2022Quote: ... DNA concentration and quality were checked via gel-electrophoresis and UV-Vis spectrophotometry (NanoDrop 1000c, Thermo Fisher Scientific, Waltham, MA, USA). DNA was stored at −20 °C until further processing.
-
bioRxiv - Molecular Biology 2022Quote: ... Substrates and reaction products were separated by agarose gel electrophoresis and the gel was stained with SYBR-Gold (S11494, Thermo Fisher).
-
bioRxiv - Molecular Biology 2023Quote: ... The remaining ∼10% of the cell lysate was then homogenized into a denaturing buffer solution containing 4X NuPAGE™ LDS Sample Buffer in preparation for polyacrylamide gel electrophoresis (Invitrogen™ NuPAGE™ ...
-
bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of whole protein extracts were loaded and separated by electrophoresis on a 4–12% Bis-Tris gel (Life Technologies) and transferred to a PVDF membrane (Thermo Fischer Scientific) ...
-
bioRxiv - Plant Biology 2023Quote: ... Fragmentation was verified using agarose gel electrophoresis and RNA quantity was determined fluometrically using Qubit RNA high-sensitivity assay kit (Thermo Fisher).
-
bioRxiv - Synthetic Biology 2023Quote: ... Positive colonies were confirmed for correct assembly by enzymatic digestion (with BbsI for level 1 assemblies) followed by gel electrophoresis band size analysis (Invitrogen G401001).
-
bioRxiv - Microbiology 2023Quote: ... PCR amplicons were visualized via gel electrophoresis using 1.4% agarose gels and Gene Ruler 100bp DNA ladder (Thermo Fisher Scientific, SM0243).
-
bioRxiv - Microbiology 2023Quote: ... PCR amplicons were visualized via gel electrophoresis using 1% agarose gels and GeneRuler 1 kb Plus DNA ladder (Thermo Scientific, SM1331). Three independent replicates were performed.
-
bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
-
bioRxiv - Immunology 2023Quote: ... Integrity and purity of purified proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Coomassie G-250 staining (ThermoFisher Scientific). Purified proteins were stored at –80°C until use.
-
bioRxiv - Microbiology 2023Quote: ... Protein samples were subjected to a 4%-15% polyacrylamide SDS gel electrophoresis followed by electrotransfer to a nitrocellulose membrane (Fisher Scientific). The membrane was blocked with 3% milk ...
-
bioRxiv - Biochemistry 2023Quote: ... The purity of the ATAD2/B bromodomain was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels stained with GelCode Blue Safe protein stain (Thermo Scientific) (24,25).
-
bioRxiv - Immunology 2023Quote: ... then S-glutathionylated proteins were released and prepared for SDS polyacrylamide gel electrophoresis (SDS-PAGE) with reducing sample buffer at 100°C (ThermoFisher #39000); immunoblotting was performed with anti-PDCE2 antibodies (GeneTex #GTX109766) ...
-
bioRxiv - Microbiology 2023Quote: PCR products were verified on a 2% agarose gel electrophoresis and the expected products were subsequently purified using ExoSAP-IT PCR Product Cleanup Reagent (Applied Biosystems) or Zymoclean Gel DNA Recovery Kit (Zymo Research) ...
-
bioRxiv - Microbiology 2023Quote: ... to be separated on 8% SDS-polyacrylamide gel electrophoresis (PAGE) or subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) using Superscript IV (Invitrogen Life Technologies), to create cDNA and then DNA to amplify for sequencing.
-
bioRxiv - Molecular Biology 2023Quote: ... and analyzed by 0.8% agarose gel electrophoresis followed by staining with SYBR Gold nucleic acid gel stain (Thermo Fisher Scientific, #S11494). Fluorescent signals were detected using the Amersham Typhoon scanner 5 system (Cytiva ...
-
bioRxiv - Immunology 2023Quote: ... We validated the specificity of each primer pair with melt curve analysis and gel electrophoresis on 1% agarose gel with SB buffer and SYBR Safe DNA stain (Life Technologies). We calculated primer efficiencies from standard curves prepared from a six-step 10-fold dilution series of cDNA ...