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Citations for Thermo Fisher :
851 - 900 of 1252 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... then proteins separated on 4-12% Bis-Tris Bolt gels using electrophoresis before transfer to nitrocellulose membranes (Thermo Fisher Scientific) by iBlot (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... The digestion of DNA was determined by agarose gel electrophoresis and visualized by SYBR Safe DNA gel stain (Invitrogen, USA). The reaction samples of V2c or its variants were scaled up for Western blotting analysis ...
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bioRxiv - Cell Biology 2023Quote: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 8% or 10% acrylamide gels and BOLT MES (Invitrogen) or Tris-glycine SDS running buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... the reaction mix was cooled to 4°C and analyzed by standard agarose gel electrophoresis with 2% (w/v) Agarose in TAE Buffer with 1:20.000 SybrSafe (Thermo Fisher), followed by imaging with Odyssey Fc at 600nm.
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bioRxiv - Biochemistry 2023Quote: ... Reactions were then quenched using 10 mM EDTA and 1% SDS and analyzed by capillary electrophoresis using an Applied Biosystems 3730xl DNA Analyzer (ThermoFisher) (Fig.4b and Supplementary Fig.7) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein separation was achieved using SDS polyacrylamide gel electrophoresis (Bolt™ 4-12% Bis-Tris Plus Gel, Thermo Fisher Scientific) and transferred onto a PVDF membrane using iBlot™ 2 Gel Transfer Device (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... DNA quality was assessed with agarose gel electrophoresis (1% TBE gel) and extracts were quantified with Qubit v2.0 (Life Technologies). Double-digest restriction site-associated DNA (ddRAD ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of CSF were loaded and separated by electrophoresis in a NuPAGE 4–12% Bis-Tris gel (Invitrogen #NP0322), transferred to a nitrocellulose membrane (250 mA ...
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bioRxiv - Neuroscience 2024Quote: ... band and edited band after enhancer KO were separated by agarose gel electrophoresis prepared with SYBR Safe DNA gel stain (Invitrogen) in 1 X Tris/Acetic Acid/EDTA buffer (ThermoScientific) ...
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bioRxiv - Biochemistry 2023Quote: ... INPUT and UNBOUND (both 10 % of initial protein extract) and BOUND (IP) fraction were resolved by electrophoresis on acrylamide gels (4-12% Invitrogen) and the transferred onto nitrocellulose membrane that were saturated for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Separated protein was transferred to a 0.2⍰μm pore nitrocellulose membrane (VitaScientific) using a wet transfer electrophoresis system (Life Technologies). The resulting membrane was blocked in Odyssey PBS Blocking Buffer (LI-COR ...
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bioRxiv - Cell Biology 2024Quote: ... and XBP1s (229bp) were separated in a 3 % agarose gel by electrophoresis and stained with SYBR Safe nucleic acid gel stain (Invitrogen). Additionally ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Fluorescently labelled PCR products were then resolved by electrophoresis on an ABI 3730xl capillary sequencer (Applied Biosystems, Waltham, MA, USA). Allele sizes were scored automatically using GeneMarker v ...
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bioRxiv - Genomics 2024Quote: ... We measured sample concentrations by Qubit and assessed digestion and ligation efficiencies by gel electrophoresis on a 0.9% agarose gel and by quantitative PCR (SYBR green, Thermo Fisher).
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bioRxiv - Genomics 2024Quote: ... Products were visualized via electrophoresis on an Agilent TapeStation 4200 and quantified with Qubit dsDNA HS assay kit (Invitrogen, Q32851) on a Qubit fluorometer (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was performed at 4°C for 30 min in dark blue cathode at 150 V (Thermo Fisher Scientific, BN2007). After 30 min ...
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bioRxiv - Microbiology 2024Quote: ... RNA quality was assessed through agarose gel electrophoresis and quantified using a NanoDropTM Lite Spectrophotometer (Thermo Fisher Scientific Inc., USA).
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bioRxiv - Cancer Biology 2021Quote: ... and protein lysates were subjected to electrophoresis through SDS–PAGE or 4-12% NuPage Bis-Tris protein gels (Thermo Fisher Scientific) followed by blotting onto nitrocellulose membranes ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were fractionated by SDS-PAGE gel electrophoresis on 4–12% (or 12%) Nupage Bis–Tris gels with MOPS running buffer (#161-0788, Invitrogen, USA) and transferred to cellulose membranes (#10600016 ...
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bioRxiv - Molecular Biology 2020Quote: ... Then proteins were separated by electrophoresis in 4%–12% Bis-Tris Protein Gels and transferred to polyvinylidene difluoride membrane (Thermo Scientific). The membranes were blocked in 5% dry milk in 0.1% Tween-20 in PBS and detected with the indicated antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... BMI1 level was assessed by western blot using standard SDS-PAGE in 4%-12% gel of NuPAGE electrophoresis system (Life Technology, Thermo Fisher). Antibodies are ...
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bioRxiv - Cell Biology 2020Quote: ... H3K27me3 and H2AK119Ub modified histones were analyzed by standard SDS-PAGE in 4%-12% gel of NuPAGE electrophoresis system (Life Technology, Thermo Fisher). Antibodies are ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 500 bp (parasite) PCR products were size-selected through 0.8% agarose gel-electrophoresis with E-Gel CloneWell Gels (Thermo Fisher Scientific), followed by purification with MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Genomics 2020Quote: ... Agarose gel electrophoresis was used to assess DNA quality and the quantity and quality of DNA was evaluated by NanoDrop spectrophotometer (Thermo Scientific).
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bioRxiv - Cell Biology 2022Quote: ... samples (equivalent of 2 x 105 cells) were subjected to electrophoresis in NuPAGE Novex 4–12% Bis-Tris pre-cast gels (NP0321 from Life Technologies). The same number of cells (see FACS sorting of diploid and tetraploid cells section ...
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bioRxiv - Cell Biology 2022Quote: ... and the samples were subjected to SDS–polyacrylamide gel electrophoresis on NuPAGE 4–12% Bis-Tris gradient gels (Novex Life Technologies). The proteins were then transferred onto a nitrocellulose membrane (Millipore) ...
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bioRxiv - Genomics 2020Quote: ... samples were heated for 5min at 70°C and size-separated by electrophoresis using 1.5mm 4-12% NuPAGE Bis-Tris protein gels and 1x NuPAGE MES SDS running buffer (Thermo Fisher Scientific) for 35-50min at 200V ...
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bioRxiv - Cell Biology 2019Quote: ... 3 μl of the final reaction was subjected to electrophoresis on a 1.5% agarose gel using 2xRNA loading buffer (Thermo Fisher Scientific). A 1:1 mix of RNA and loading buffer was heated to 65°C for 5 min and then placed on ice to denature any secondary structure of RNA.
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A DNA-based optical nanosensor for in vivo imaging of acetylcholine in the peripheral nervous systembioRxiv - Bioengineering 2020Quote: ... Native PAGE was conducted to characterize DNA nanostructures by using 4% TBE gel and 1x TBE buffer at a constant voltage of 200 V (electrophoresis system Thermo Scientific) in an ice water bath ...
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bioRxiv - Neuroscience 2019Quote: ... After the last wash PBS was completely aspirated and proteins were eluted from the beads and samples prepared for loading onto electrophoresis gels by adding 150uL-1X sample buffer (IP buffer 100ul buffer + 35uL of 4X NuPAGE™ LDS Sample Buffer ThermoFisher cat# NP0007 ...
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bioRxiv - Genomics 2019Quote: ... DNA integrity was assessed by electrophoresis and concentration was determined using the Nanodrop ND-1000 spectrophotometer (Thermo Scientific cat.# ND-1000) and Qubit fluorometer (Thermo Scientific cat.# Q33226) ...
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bioRxiv - Biochemistry 2019Quote: ... purity and integrity of the RNA were evaluated by agarose gel electrophoresis and on a ND-2000 spectrophotometer (Thermo Scientific, USA). For each RNA sample ...
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bioRxiv - Genomics 2020Quote: ... The integrity and concentration of RNA was analysed by agarose gel electrophoresis and using the Nanodrop ND-2000 spectrophotometer (Thermo Scientific), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... prior to SDS-PAGE gel electrophoresis on 1.5 mm NuPAGE Novex 4-12 % Bis-Tris Protein Gels (Thermo Fisher Scientific, USA), using Mini Trans-Blot Cell system (BioRad ...
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bioRxiv - Cell Biology 2019Quote: ... Samples with equal protein amounts were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using standard methods (89) and electroblotted onto polyvinylidene fluoride (PVDF) membrane (ThermoFisher, 88518). After blocking in Tris-buffered saline with 0.1% Tween (TBST ...
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bioRxiv - Immunology 2019Quote: ... 30 or 40 µg of total protein from tissue lystate was denatured and separated by SDS polyacrylamide gel electrophoresis and transferred to a PVDF membrane (IB401002, Life Technologies). Membranes were cut along the 55 kDa ladder marker and blocked with either 5% BSA or 5% dry skim milk in TBST for 1 hour at room temperature ...
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bioRxiv - Genetics 2019Quote: ... The protein samples were then size-separated by electrophoresis on a gradient Bolt 4-12% Bis-Tris plus gel (Invitrogen NW04120BOX), transferred on a polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA integrity was assessed using 1% (w/v) agarose gel electrophoresis and was quantified using a NanoDrop ND-1000 (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Alleles were size separated by electrophoresis on an ABI PRISM® 3130 sequencer and scored with GeneMapper version 5 (Applied Biosystems). Among the 236 adult trees in the experimental garden ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We selected libraries within the size range of 450-500 bp using a 2% E-gel on an E-Gel Electrophoresis System (Invitrogen, Canada), and re-amplified the size-selected libraries using a NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted in 6X Laemmli sample buffer and resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Thermo Scientific). Blots were probed with the indicated primary antibodies and with horseradish peroxidase (HRP ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We assessed DNA quality by 0.8 % agarose gel electrophoresis and quantity by fluorometry using a Qubit 4.0 Fluorometer (Invitrogen, Carlsbad, CA, USA). Samples were individually barcoded and pooled into a single library with the NEB Next Ultra DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: Cultured cells were collected and lysed with RIPA buffer and a total of 20 μg protein was separated by SDS-polyacrylamide gel electrophoresis using 4-12% NuPAGE Bis-Tris gels (ThermoFisher Scientific), blotted to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the BRPF1 bromodomain was verified by SDS-polyacrylamide gel electrophoresis stained with GelCode Blue Safe protein stain (Thermo Scientific).
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of each sample was separated in 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (45-004-110, Fisher Scientific) in ice-cold transfer buffer (25 mM Tris-base ...
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bioRxiv - Cancer Biology 2020Quote: ... The amplified products were then resolved by electrophoresis using a 1.2% E-gel containing a SYBR Safe stain (Thermo Fisher Scientific), which was visualized on an E-Gel Imager Blue-Light Base (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: ... Protein concentration was determined by absorption spectroscopy and purity by SDS-PAGE electrophoresis on 4-12% NuPAGE gels in MES buffer (Life Technologies).
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bioRxiv - Biochemistry 2021Quote: ... Deglycosylation on the standard proteins was analyzed by gel electrophoresis on 15% SDS-PAGE gels and the gels stained by Coomassie Brilliant Blue R-250 Dye (Cat. 20278, Thermo Scientific). The optimal conditions were applied to study limited deglycosylation on LLC-MK2 cellular glycoproteins upon induction of ER stress ...
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of protein (20-50 μg) were loaded and were separated by electrophoresis in 4 to 12% Bis-Tris Gel (ThermoFisher Scientific), transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Plant Biology 2021Quote: ... Nuclei were then processed with the CometAssay® Kit for electrophoresis and stained with SYBR Gold (Thermo Scientific, Waltham, MA, USA). Slides were examined under an Olympus BX53 fluorescence microscope (Olympus ...