Labshake search
Citations for Thermo Fisher :
1101 - 1150 of 1252 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... Methylation was analyzed by DNA gel electrophoresis of 600 ng of DpnI- or MboI-digested plasmid DNA and GeneRuler 1 kb DNA Ladder (Thermo Fisher SM0313). All annotated plasmid sequences for the transactivator- and reporter-expressing plasmids are available in the Haynes Lab Benchling database (https://benchling.com/hayneslab/f_/mrI2t4mM-collection-dal-e/).
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bioRxiv - Neuroscience 2021Quote: ... Samples were boiled at 95°C for 5min and subsequently separated by polyacrylamide gel electrophoresis on a 4-12% pre-cast NuPage Bis-Tris protein gel (Thermo Fisher Scientific). Finally ...
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bioRxiv - Plant Biology 2021Quote: ... Amplifications were developed separately for each one and size analyses by capillarity electrophoresis were developed in SECUGEN S.L (ABI PRISM 310 Genetic Analyzer. PE Applied Biosystems, California, USA). The analysis of all capillarity electrophoresis data was performed by Peak Scanner Software 2 version 2.0.
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bioRxiv - Bioengineering 2021Quote: Samples from time course experiments and the truncation screening were prepared for sodium dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) by adding 12 µL of NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) and 3 µL of tris(2-carboxyethyl)phosphine (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... DNA samples were run on 1% agarose gel electrophoresis for quality control and quantified using the NanoDrop spectrophotometer (Thermo Fisher Scientific Inc.). For each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... After incubation for 1 h at 37 °C the DNA products were analyzed by electrophoresis on a denaturing polyacrylamide gel (10%) and detected by chemiluminescent detection (Thermo Fisher Scientific). To semi-quantify the level of dCTP incorporation ...
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bioRxiv - Genomics 2022Quote: ... The quality and concentration of the extracted genomic DNA was assessed using 1% agarose gel electrophoresis and a Qubit fluorimeter (Invitrogen, Carlsbad, CA). This high-quality DNA was used for subsequent Nanopore and Illumina sequencing ...
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bioRxiv - Immunology 2022Quote: ... and 25ug of cell lysates from each condition were resolved by 8% SDS-PAGE with NuPAGE electrophoresis system (Invitrogen/Thermo Fisher Scientific). Proteins were then transferred to PVDF membrane (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and 25ug of cell lysates from each condition were resolved by 8% SDS-PAGE with NuPAGE electrophoresis system (Invitrogen/Thermo Fisher Scientific). Proteins were then transferred to PVDF membrane (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 10 colonies of each npc1 heterozygous mutant were amplified and double checked by 1% agarose gel electrophoresis and Sanger sequencing with 3730xl DNA Analyzer (Thermo Fisher Scientific). Finally ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in either homemade 7% or 4-12% gradient Bis-Tris polyacrylamide gel (Thermo Fisher Scientific) using 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products and products of restriction digests were purified using agarose gel electrophoresis and the GeneJET gel extraction kit (Thermo Fisher Scientific). DNA sequences were analyzed by DNA sequence service of Fasmac Co. ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate Bis-Tris polyacrylamide gel electrophoresis (SDS-PAGE) (NuPAGE™ 4 to 12%, Bis-Tris precast polyacrylamide gel, Invitrogen # NP0323BOX) and afterward transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) at 150V for 2h using NuPAGE 2-(N-morpholino)ethanesulfonic acid SDS running buffer (NP0002, Thermo Fisher Scientific), NuPAGE Antioxidant (NP0005 ...
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bioRxiv - Cancer Biology 2023Quote: ... Polyadenylation and integrity were assessed by gel electrophoresis in denaturing conditions and RNA was quantified with a Qbit fluorometer (Thermo Fisher Scientific). Purified RNA was resuspended in water at 1-10 μg·mL-1 and stored at -80°C until used.
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bioRxiv - Microbiology 2023Quote: ... to be separated on 8% SDS-polyacrylamide gel electrophoresis (PAGE) or subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) using Superscript IV (Invitrogen Life Technologies), to create cDNA and then DNA to amplify for sequencing.
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bioRxiv - Cell Biology 2023Quote: ... PCR products were separated on a 1% agarose gel electrophoresis and NGS Libraries were generated using an enzymatic DNA fragmentation approach with Ion Xpress Plus Fragment Library Kit (Thermo Fisher Scientific). Sequencing was performed using an Ion Torrent S5XL platform with an Ion 540 chip ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell-free synthesized protein was visualized by Coomassie blue staining after protein gel electrophoresis using pre-casted 4-12% Bis-Tris gradient gel (Invitrogen, Waltham, MA). Purified protein concentration was measured by Bradford Assay.
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bioRxiv - Genomics 2023Quote: ... DNA concentration and quality were examined by electrophoresis on a 2% (w/v) agarose gel and a Qubit 3.0 fluorometer (Thermo Scientific, Wilmington, USA), respectively.
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bioRxiv - Microbiology 2023Quote: ... aeruginosa and Salmonella organisms were prepared and separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) as described (18) with a Novex X-Cell Surelock minicell system (Invitrogen, Carlsbad, CA). Tris-bis-polyacrylamide gels (12.5% ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Pooled samples of protein extracts (80 μg) were separated using electrophoresis on 4– 12% NuPage Bis-Tris gels with MOPS buffer (Invitrogen, Carlsbad, CA) and then transferred to Immobilon-P membranes (Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were separated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride (PVDF) membrane (Thermo Fisher Scientific). The membranes were blocked in 5% nonfat milk (RPI International ...
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bioRxiv - Genomics 2023Quote: All PCR products were assessed by gel electrophoresis and quantified using the Qubit™ dsDNA HS Assay kit (Cat. #Q32854; Thermo-Fisher Scientific). Primers were synthesized by Integrated DNA Technologies® and diluted to 10uM in 0.1x TE buffer ...
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bioRxiv - Genetics 2023Quote: ... We performed the PCR amplification with combinations of three multiplex panels and subjected the amplified products to capillary electrophoresis for DNA fragment analysis in ABI 310 Genetic Analyzer (Applied Biosystems, USA). We manually scored allele sizes in Gene Mapper software version 4.0 (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: High molecular weight westerns for MLL3 and MLL4 were conducted using a NuPage SDS-PAGE and Transfer system with an XCell electrophoresis unit according to manufacturer’s protocols with several modifications (Thermo Fisher, CAT#E10002). In brief ...
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bioRxiv - Genetics 2023Quote: ... of the complementary T7/M13 primer during PCR and hence detection during capillary electrophoresis on ABI 3730 Genetic Analyzer (Applied Biosystems Inc.).
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bioRxiv - Biophysics 2023Quote: ... in the presence of different concentrations of uranyl acetate (0–1000 µM) were analyzed with blue native polyacrylamide gel electrophoresis (BN- PAGE) using the Invitrogen system (ThermoFisher Scientific, USA). Thus ...
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bioRxiv - Cell Biology 2023Quote: ... or 10μg EV proteins were separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis (10-12% polyacrylamide, SureCast™ Acrylamide Solution, ThermoFisher Scientific, MA) and transferred onto PVDF membranes ...
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bioRxiv - Plant Biology 2023Quote: ... The quality and quantity of isolated RNA samples were assessed by performing 1% agarose gel electrophoresis and NanoDrop 2000C (Thermo Fisher, USA). The high-quality RNA samples were outsourced for RNA sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were transferred into fresh tubes and 50 µg of each sample was subjected to native gel electrophoresis to reveal the various proteasome complexes (30S, 26S, 20S) using a NativePAGE™ 3 to 12% Bis-Tris gel (Invitrogen, BN1001BOX) in XCell™ SureLock™ Mini-Cell system (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... The purity of viruses was routinely checked by silver staining (Pierce, Germany) after gel electrophoresis (NovexTM 4-12% Tris-Glycine, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: Protein blot analysis was performed following electrophoresis of reduced samples using NuPAGE 4 to 12% Bis-Tris Mini Polyacrylamide Gels (Thermo Scientific, #NP0322BOX). Protein was then transferred to PVDF membranes using the iBlot 2 Dry Blotting System from Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and their purity validated by sodium dodecyl sulfate -polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blot (WesternBreeze Chromogenic Immunodetection System, Thermo Fisher Scientific) using antibodies against His6xtag.
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bioRxiv - Cell Biology 2023Quote: ... Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific), then transferred to 0.45 µm nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were separated by dodecyl-sulfate polyacrylamide gel electrophoresis and transferred to Amersham™ Protran® nitrocellulose membranes or PVDF membranes (Invitrogen). The membranes were blocked with 5% non-fat dry milk-TBST (10 mM Tris-HCL [pH 8.0] ...
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bioRxiv - Immunology 2023Quote: ... to amplify immunoglobulin heavy and light chain variable regions and yielding variable regions were confirmed by using 96 well E-gel agarose electrophoresis (Thermo Fisher Scientific). Purified PCR amplicons were then sequenced with forward and reverse primers and contigs of the amplified immunoglobulin sequences were made by the Duke automated sequence analysis pipeline (Duke ASAP) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein material was resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) in 12-well gels (Invitrogen, 4-12% Bis-tris BOLT gels) in 0.9x MOPS-SDS buffer (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... The purity of all newly synthesized RNAs was detected using UREA-polyacrylamide gel electrophoresis (PAGE) which was stained with SYBR™ Gold (Invitrogen, S11494). The gels were finally visualized on a ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated using Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) in 8% polyacrylamide gel together with PageRuler protein ladder (Thermo Fisher Scientific) and transferred onto polyvinylidene difluoride membrane (PVDF) ...
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Loss of the mitochondrial citrate carrier, Slc25a1/CIC disrupts embryogenesis via 2-HydroxyglutaratebioRxiv - Developmental Biology 2024Quote: ... Equal amount of protein lysate was loaded and separated in an SDS-polyacrylamide gel electrophoresis using Novex™ 4-20% Tris-Glycine Mini Gel (Invitrogen, XP04200PK2) and transferred to PVDF membrane (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA quality and concentration were determined by MOPS-formaldehyde denaturing agarose gel electrophoresis and Qubit RNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genetics 2024Quote: The quality of the purified DNA was assessed through gel electrophoresis on a 0.8% agarose gel and a Thermo Scientific™ NanoDrop Lite spectrophotometer (Thermo Scientific, UK). The DNA in each sample was quantified with a Qubit 4 Fluorometer (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... The final eluate of 2 rows was used for SDS gel electrophoresis under reducing conditions on a 4–12% bis-tris polyacrylamide gel (Life Technologies, NP0321BOX), which was blotted onto nitrocellulose membranes (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... The amplicons were checked by electrophoresis and 25 µl of each pooled PCR product were purified and normalized using SequalPrep Normalization Plate Kit (Invitrogen GmbH, Karlsruhe, Germany). We then pooled the samples and the mock community and proceeded for a single library preparation ...
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bioRxiv - Developmental Biology 2021Quote: ... The final library size distribution was validated with capillary electrophoresis and quantified with a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, P11496) and qPCR ...
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bioRxiv - Microbiology 2021Quote: ... The resulting cDNA was either amplified by PCR and detected by gel electrophoresis or quantified by qRT-PCR using the PowerUp SYBR green qPCR kit (Applied Biosystems, catalog # A25918).
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were visualised using agarose gel electrophoresis with a 2% TAE gel containing a 1:10,000 dilution of SYBR® Safe DNA Gel Stain (Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Neuroscience 2021Quote: ... The concentrated RNA (10-15 µg per sample) was then size fractionated by electrophoresis using 15% polyacrylamide-urea gels (ThermoFisher Scientific, catalog # EC68852BOX) run at 200 volts in TBE (89 mM Tris-borate ...
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bioRxiv - Molecular Biology 2020Quote: Western blot analysis was carried out using standard procedures for SDS-PAGE electrophoresis on 4-12% NuPage gels in MOPS buffer (Invitrogen, Carlsbad, CA, USA), then transferred to nitrocellulose membranes (Whatman ...
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bioRxiv - Genetics 2020Quote: Proteins eluted from the beads were separated by SDS/PAGE electrophoresis on a 10% polyacrylamide gel and stained with Colloidal blue silver (ThermoFisher Scientific, USA; 70). SDS-PAGE gel lanes were cut into 2-mm slices and subjected to in-gel reduction with dithiothreitol ...