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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and Mini- Protein Electrophoresis Cells (Invitrogen, Thermo Fisher Scientific). Prestained Protein Ladder (NuPAGE ...
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bioRxiv - Biochemistry 2023Quote: ... and Mini- Protein Electrophoresis Cells (Invitrogen, Thermo Fisher Scientific). Prestained Protein Ladder (NuPAGE ...
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bioRxiv - Microbiology 2023Quote: ... Cell protein lysates were quantified by Bradford assay (ThermoFisher), then separated by Tris-Glycine SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were expressed in Expi293F cells (Life technologies). Full-length spike proteins were cloned into the pCAGGS mammalian expression vector as previously described79,80 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 3-8% Tris-Acetate precast gels (Novex, Life Technologies, Invitrogen) run for ∼1hr at 170V in 1x Tris-Acetate running buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 3-8% Tris-Acetate precast gels (Novex, Life Technologies, Invitrogen) run for ∼1hr at 170V in 1x Tris-Acetate running buffer ...
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bioRxiv - Immunology 2021Quote: Membrane proteins from ZIKV-infected or uninfected JEG-3 were isolated by Mem-PER Plus Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... 10 μg of extracted proteins (n = 3) were solubilized in 1 × NuPage® LDS Sample buffer (Invitrogen, USA) and was run in a 15 well 12% Tris-Gly precast gel (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples containing 15 µg of protein were separated on 3–12% Bis-Tris Novex NativePAGE gel (Life Technologies). The relative levels of the assembled respiratory complexes I-V were assessed by western blot with following antibodies ...
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bioRxiv - Biophysics 2020Quote: ... 3 μg of protein was loaded to each well of a NuPAGE 12% Bis-Tris gel (Invitrogen, NP0343BOX) and stained with Coomassie (Extended Data Fig ...
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bioRxiv - Bioengineering 2019Quote: ... tissues were wash 3 times with PBS and incubate with protein blocking solution (Thermo Fisher Scientific, Waltham, MA). Then tissues were incubated in 1:150 diluted PSMA primary antibody (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... S6 and subjected for BN-PAGE electrophoresis using the NativePAGE Novex 3–12% Bis-Tris Protein Gels (Invitrogen), following the protocol provided by Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher) through gold coated capillary needles that were prepared in-house30 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were migrated for 2-3 h at 120 V in NuPAGE MOPS SDS running buffer (#NP0001, Invitrogen) and transferred onto a PVDF membrane (#1704156 ...
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bioRxiv - Microbiology 2023Quote: ... the supernatants were electrophoresed on NativePAGE Novex 3–12% Bis-Tris protein gels as per manufacturer’s instructions (Invitrogen). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... HEK293T cells and LHL2/3 cells were maintained in DMEM (Gibco, Grand Island, NY, United States) medium supplied with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... 16HBE cells were suspended at a density of 3 x 105 cells/ml in M199 (Gibco) plus 10% FCS ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were seeded at 3×103 cell/cm2 and maintained in LHC-9 media (ThermoFisher 12680013) at 37 °C with 85% humidity and 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... Cell proliferation was quantified after 3 days of culture using CyQUANT Cell Proliferation Assay tests (ThermoFisher) according to the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2023Quote: ... 1% P/S at a cell density between 3 × 105 and 2.5 × 106 cells/ml (GIBCO). Cells were always handled and maintained under sterile conditions (tissue culture hood ...
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bioRxiv - Neuroscience 2020Quote: ... Virus-containing supernatant was harvested three days after transfection with Lipofectamine 2000 (Thermofisher). This supernatant was then cleared from cell debris by centrifugation at 3500 rpm for 15 min and filtered through a 0.45 µm filter (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... to wash the virus in Opti-MEM™ Medium (ThermoFisher Scientific, Darmstadt, Germany) and concentrate to a final volume of 150 μl.
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bioRxiv - Neuroscience 2020Quote: ... They were reprogrammed into iPSCs using the replication incompetent Sendaï virus kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The virus pellet was then re-suspended in PBS (Thermo Fisher Scientific, 10010015) containing 25 mM HEPES buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... using the Taqman Fast Virus 1-stepMaster Mix enzyme (Thermo Fisher, Waltham, MA). The limit of detection of this assay is estimated to be 200 genome equivalents/ml saliva or swab fluid ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
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bioRxiv - Microbiology 2021Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific; 4444434) for a total reaction volume of 20 μl in 96-well PCR plates (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) in a total reaction volume of 10 μl ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... ten-fold dilutions of virus were prepared in diluent medium (Opti-MEM (Invitrogen) with 2% FBS (Gibco ...
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bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...
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bioRxiv - Genomics 2021Quote: ... hiPSCs were generated from primary fibroblasts using Sendai virus reprogramming kit (ThermoFisher Scientific), yielding 2-3 different clonal lines for each individual ...
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bioRxiv - Microbiology 2022Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Immunology 2022Quote: ... the master mix used was TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Manufacturer’s protocol was followed to add the appropriate volumes of master mix ...
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bioRxiv - Microbiology 2022Quote: Purified virus particles were incubated with different concentrations of BS3 cosslinkers (ThermoFisher Scientific) for 30 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and Toledo virus was conjugated with DMSO-dissolved AF647–N-hydroxysuccinimide ester (Invitrogen) with constant agitation at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml, Gibco) 24h later ...
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bioRxiv - Neuroscience 2022Quote: ... PBMCs were expanded and reprogrammed using Sendai virus vector (CytoTune-iPS 2.0, Invitrogen) using a feeder-free system (Sommer et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (ThermoFisher Scientific). Real-time PCR was performed using TaqMan assays (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Nuclease treatment of virus stocks was performed at room temperature by DNase (Ambion), RNase (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The virus-containing media were filtered through a 0.45 µm filter (ThermoFisher Scientific) and mixed with fresh media at a 1:1 ratio before adding to pre-seeded target cells ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Immunology 2023Quote: ... RNA from virus stocks was purified using TRIzol Reagent (Thermo Fisher Scientific, #15596026). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was prepared using Moloney Murine Leukemia Virus reverse transcriptase (Thermo Fisher Scientific) with Random Hexamers (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... the media was changed to virus production medium containing Opti-MEM I (Gibco) + 5 % FBS + 1:100 Glutamax + 1:100 MEM-NEAA (Gibco ...
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bioRxiv - Microbiology 2021Quote: 3 μg of in vitro-transcribed RNA derived from the pCrPV-3 or chimeric clones was transfected into 3 × 106 S2 cells using lipofectamine 2000 reagent (Life Technologies) per the manufacturer’s instructions.