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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: HEK293 wild type (ATCC, #CRL-1573) and HEK293 Flp-in T-REx (ThermoFisher, #R78007) wild type cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 Flp-In T-REx cells (or HEK293 T-REx; Thermo Fisher Scientific, R78007) and HEK293 cells [American Type Culture Collection (ATCC) ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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bioRxiv - Microbiology 2020Quote: ... once and then 100 μl of 1:300 diluted secondary antibody (ThermoFisher Cat # A-21091 for Fc-tag and ThermoFisher Cat # MA1-21315-647 for polyhistidine-tag ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Molecular Biology 2021Quote: ... and PToV Hemagglutinin Esterase non-binding (F271A) (1176bp) fused to human IgG-Fc followed by a 6xHis tag in the Insect expression vector pFastBac1 (ThermoFisher) was kindly provided by Colin Parrish (PToV-HE_S46A-Fc_Hisx6/pFastBac1 ...
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bioRxiv - Cell Biology 2023Quote: ... ALFA-FAM134s were targeted directly using an anti-ALFA nanobody carrying a 5xR1 docking strand (5-TCCTCCTCCTCCTCCTCCT-3’) (Massive-TAG-Q Anti-ALFA Custom, Massive Photonics, GmbH) which was diluted in permeabilization/blocking buffer (10% FCS (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The monoclonal HA-epitope tag antibody 2-2.2.14 (Invitrogen, catalog no. 26183) was used for detection of hemagglutinin-tagged proteins (1:5000 dilution) ...
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bioRxiv - Immunology 2022Quote: ... Undigested IgG and Fc fragments were removed by a 2 h incubation with CaptureSelect IgG-Fc resin (Thermo Fisher Scientific). Resin was spun down and the supernatant run on a Superdex 200 increase 10/300 GL column (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: HEK293-FT (Thermo Fisher Scientific) were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... HEK293 Freestyle suspension cells (ThermoFisher), and immortalized human cord blood-derived mesenchymal stromal cells (cbMSCs ...
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bioRxiv - Neuroscience 2020Quote: HEK293-FT (RRID:CVCL_6911, Thermo Scientific) cells were grown in DMEM containing 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: HEK293-FlpIn cells (ThermoFisher Scientific) were transfected with empty vector (negative control ...
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bioRxiv - Biochemistry 2022Quote: HEK293 FlpIn-TRex cells (Invitrogen) were cultured in DMEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: HEK293 FlpIn-TRex cells (Invitrogen) were cultured in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293-FT (Thermo Fisher Scientific) were cultured in DMEM with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293-FT (Thermo Fisher Scientific) in DMEM with 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293-F cells (ThermoFisher R79007) were transfected with the transfer plasmid and the packaging plasmids (Addgene plasmids #12263 and #8454 ...
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bioRxiv - Immunology 2020Quote: ... The secondary antibodies: sheep anti-mouse- and sheep-anti rabbit-HRP were acquired from Invitrogen/Bio-Rad (Hercules ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were subjected to drug selection in the medium with 2 μg/ml puromicin (Invitrogen) for 14 days ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells were seeded at 2 ×105 cells per well in six well plates (Thermo Fisher) in DMEM/10% FBS ...
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bioRxiv - Physiology 2024Quote: ... These cells were generated by transfecting HEK293 cells using Lipofectamine 2000 (Invitrogen; 2 μl per well) with D1ER cDNA (0.5 μg/well ...
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bioRxiv - Microbiology 2021Quote: ... Permeabilized cells were probed with a monoclonal antibody against the HA-tag epitope (1:1000; HA Tag mAb 2-2.2.14, Thermo Fisher Scientific, Planegg, Germany) to detect SARS-2-S protein ...
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bioRxiv - Biophysics 2022Quote: ... in blocking buffer followed by 10 min incubation at room temperature with goat anti-rabbit IgG/horseradish peroxidase-conjugate (1:10,000 dilution of 1 mg/mL stock in blocking buffer) (A16110, Thermo Fisher Scientific). The blot was developed using enhanced chemiluminescence detection reagents (Pierce™ ECL Western Blotting Substrate ...
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bioRxiv - Cell Biology 2022Quote: ... were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting using a rabbit polyclonal anti-syncollin antibody (Hodel and Edwardson, 2000) followed by a horseradish peroxidase-conjugated goat anti-rabbit antibody and enhanced chemiluminescence imaging (Thermo Fisher). Band intensities were quantified (in A.U. ...
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bioRxiv - Developmental Biology 2022Quote: ... the blots were incubated in secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in TBST (1:2000 – 10000; Thermo Fisher Scientific). The blots were then washed and detected using SuperSignal West Dura Extended Duration Substrate (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-Mouse IgG (H+L) and goat anti-Rabbit IgG (H+L) conjugated to horseradish peroxidase (1:10,000, Thermo Fisher Scientific). Loading control was obtained incubating with anti-actin-horseradish peroxidase conjugated (1:100,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed in TBS-T buffer and then incubated for an hour at room temperature with the corresponding horseradish peroxidase-conjugated preadsorbed secondary antibody (1/2000, blocking solution corresponding, Molecular Probes). Membranes were washed in TBS-T buffer ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: ... Blots were washed with 0.05% TBS-T and then incubated with horseradish peroxidase-conjugated anti-mouse and anti-rabbit secondary antibodies (Thermo Fisher Scientific) in 0.05% TBS-T at a dilution of 1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies were used: Horseradish peroxidase- conjugated anti-mouse and anti-rabbit antibodies (1:5000; 31430 and 31460, Thermo Fisher).
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed in TBS-T buffer and then incubated for an hour at room temperature with the corresponding horseradish peroxidase-conjugated pre-adsorbed secondary antibody (1:5000, blocking solution corresponding, Molecular Probes). Membranes were washed in TBS-T buffer and immunoreactive bands were visualized with the SuperSignal West Pico Chemiluminescent Substrate (Pierce) ...
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bioRxiv - Cancer Biology 2020Quote: ... or Insulin Receptor beta (ab69508) overnight at 4°C followed by anti-rabbit secondary antibody conjugated to horseradish peroxidase (ThermoFisher Scientific). Proteins were detected by Luminata Crescendo immunoblot HRP substrate (WBLUR0500Millipore Corporation ...
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bioRxiv - Neuroscience 2020Quote: ... After incubation with primary antibodies membrane was washed with PBS-T three times on a platform rocker and incubated with horseradish peroxidase-conjugated secondary antibody diluted in PBS-T 1:2000 (Thermo Scientific) for 1 hour at RT on a platform rocker ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... the membrane was washed with TBST four times and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (Invitrogen, polyclonal, #656120) or goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... An anti-rabbit immunoglobulin G conjugated to horseradish peroxidase was used as the secondary antibody and detected using the ECL chemiluminescence detection system (Thermo Scientific) in a chemiluminescence imager (Vilber Lourmat).
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bioRxiv - Microbiology 2020Quote: ... the bound antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% milk and goat anti-rabbit IgG (H1L) horseradish peroxidase (HRP)-conjugated secondary antibody (1:10,000, Thermo Fisher Scientific 31460) in TBST were used.
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bioRxiv - Cancer Biology 2022Quote: ... Blots were then washed and incubated with Horseradish peroxidase-conjugated secondary Abs and bands were visualized using enhanced reagent (Thermo Scientific) and imaging either on film or an ImageQuant LAS 4000 imager (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2022Quote: ... Immunoreactive proteins were visualized by incubation with appropriate horseradish peroxidase-linked secondary antibodies (1:10000 dilution) followed by incubation with the chemiluminescence system (Thermo Scientific) according to the manufacturer’s protocol and revelation was done using the ChemiDoc XRS+ system (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: ... primary antibody followed by incubation of horseradish peroxidase-linked goat anti-rabbit or anti-mouse secondary antibody (1: 5,000, Thermo Fisher Scientific). Antibody binding was detected using the enhanced chemiluminescence immunoblotting detection system (Perkin Elmer LLC WESTERN LIGHTNING PLUS-ECL ...
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bioRxiv - Microbiology 2022Quote: ... The expression of myc tagged TaPHB-1 was detected using horseradish-peroxidase (HRP) conjugated anti-mouse secondary antibody (Pierce) by SuperSignal West Pico chemiluminescent substrate (Thermo Scientific) in G ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed three times with 0.5% BSA–PBS and incubated with a horseradish peroxidase-conjugated goat anti-rabbit antibody (0.3 μg/ml, cat# 31460; Thermo Fisher Scientific) in 1% BSA–PBS for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and polyclonal antibody raised against Erg6 (kind gift from G. Daum, Universität Graz) were used and subsequently with horseradish peroxidase-conjugated secondary antibodies (Invitrogen, Thermo Fisher). Enhanced chemiluminescence (Amersham ...
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bioRxiv - Neuroscience 2021Quote: ... overnight at 4 °C and with horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed five times with PBS and then incubated with horseradish-peroxidase-conjugated goat-anti-rabbit secondary antibody (Invitrogen # A16104) diluted 1:1000 in milk shaking for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were washed four times with 0.5% BSA/PBS and incubated with a horseradish peroxidase-conjugated goat anti-rabbit antibody (1:3,000; Thermo Fisher Scientific #31460) in 1% BSA/PBS for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... The secondary anti-mouse and anti-rabbit horseradish peroxidase-conjugated antibodies and the Alexa Fluor 488- and 555-congugated antibodies were from Thermo Fisher Scientific.
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bioRxiv - Plant Biology 2020Quote: ... MpPHOT proteins were detected with rabbit antibody raised against the custom-made synthetic peptide (C+VDERAPPSKGSAKE) (Eurofins Genomics) and horseradish peroxidase-conjugated anti-rabbit IgG (Thermo Scientific). Both antibodies were used at dilution of 1:2000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunocomplexes were incubated with the appropriate horseradish peroxidase-conjugated secondary antibody and detected using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) or Radiance Plus Chemiluminescent Substrate (Cambridge Bioscience ...