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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Slides were next incubated in biotinylated secondary antibody for 1 hour at room temperature followed by incubation in fluorophore tagged horseradish peroxidase (Alexa Fluor; Thermofisher). Sections were washed thrice in PBS-T and counterstained with RNAscope DAPI for 30 seconds ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 times for 10 minutes with 0.1% PBS-Tween 20 and incubated with the appropriate horseradish-peroxidase-conjugated secondary antibodies (Thermo Scientific) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... containing 150 mM NaCl and 0.1% Tween-20 (TBST) and were incubated with horseradish peroxidase-conjugated goat anti-rabbit (31460, RRID: AB_228341, Thermo Fisher Scientific) or anti-mouse (31430 ...
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bioRxiv - Microbiology 2021Quote: ... Blots were then incubated with anti-rabbit IgG conjugated with horseradish peroxidase (Cell Signalling) or Alexa Fluor Plus 800 (Thermofisher), or anti-mouse IgG conjugated with Alexa Fluor Plus 680 (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... and detected using horseradish peroxidase-conjugated goat-a-mouse or goat-a-rabbit secondary antibodies followed by chemiluminescence (Thermo Scientific). Chemiluminescent membranes were exposed to X-ray film for 5–180 s and developed with a Konica SRX-101A film processor ...
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bioRxiv - Cell Biology 2021Quote: ... The immunoblots were analysed using primary antibodies incubated overnight at 4°C and secondary antibodies linked to horseradish peroxidase (HRP) (Invitrogen), and after each step immunoblots were washed 4x with TBST ...
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bioRxiv - Cell Biology 2021Quote: ... incubated with goat anti-mouse or goat anti-rabbit secondary antibody conjugated to horseradish peroxidase (Thermo Fisher Scientific, 1:5,000), washed ...
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bioRxiv - Bioengineering 2022Quote: ... conjugated to horseradish peroxidase (HRP) were used for detection of proteins using ECL Western Blot Substrate Reagent (Thermo Fisher Scientific). Images were acquired using a Chemidoc Imaging System and analyzed with Image-Lab software (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... Reactive bands were visualised using goat anti-mouse IgG or goat anti-rabbit IgG secondary antibodies conjugated to horseradish peroxidase (Invitrogen) and the LiteAblot Plus Enhanced Chemiluminescent Substrate (EuroClone).
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... and rabbit anti-Calnexin (Stressgene, #SPA-860) followed by incubation with horseradish peroxidase-conjugated secondary antibodies and ECL Plus substrate (ThermoFisher, Pierce ECL Plus Western Blotting Substrates) ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was washed 4 times with 1X TBS containing 0.1% (v/v) Tween-20 and incubated with 1/5,000 diluted goat anti-rabbit horseradish peroxidase-conjugated secondary antibody (Thermo Scientific) in 5% milk TBS for 1 hour at room temperature with shaking ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies specific to the species of the primary antibody conjugated to horseradish peroxidase were added to the membranes: anti-rabbit (Invitrogen) and anti-mouse (EMD Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... respectively) Primary antibodies were detected using Horseradish Peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:5,000 dilution, Invitrogen #A16110) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with a horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody (1:1000; Thermo Fisher Scientific, RRID:AB_228341) or a HRP-conjugated goat anti-mouse secondary antibody (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... the membrane was probed with HIV-Ig as the primary antibody and corresponding secondary antibody conjugated to horseradish peroxidase (goat anti-human IgG, Invitrogen). Gag-specific bands were detected after incubation with SuperSignal West Pico chemiluminescent substrate (Pierce ...
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bioRxiv - Biochemistry 2020Quote: ... and then for 1 h with a 1/10 000 dilution of anti-mouse– horseradish peroxidase conjugate (Thermo Fisher Scientific). His-tagged proteins bound to the membrane by virtue of its interaction with lipid ...
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bioRxiv - Biophysics 2020Quote: ... The membranes were washed three times with PBST and then incubated with horseradish peroxidase (HRP)-goat anti-mouse secondary antibody (1 : 5000 dilution, Invitrogen) for 1 hr at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... The secondary immunoglobulins conjugated to horseradish peroxidase were visualized using enhanced chemiluminescence with a commercial kit (ECL Pierce Plus, ThermoFisher) or a homemade solution (100 mM Tris-HCl pH 8.5 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... this complex was incubated for 90 minutes with 50 μl horseradish peroxidase-conjugated antibody (anti rabbit, IgG, Fisher Scientific; NA934) diluted in 50% PBS ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies including goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated (31430 thermo) and anti-mouse IgA HRP-conjugated (626720 Invitrogen) were diluted 1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Secondary horseradish peroxidase(HRP)-conjugated goat anti-mouse (31430) and goat anti-rabbit (31460) antibodies were purchased from Thermo Fisher Scientific.
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bioRxiv - Neuroscience 2022Quote: ... Protease and Phosphatase Inhibitor Cocktail (100x) and enhanced chemiluminescent (ECL)-horseradish peroxidase (HRP)HRP substrate were purchased from Thermo Fisher Scientific (Kiryat Shmona ...
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bioRxiv - Plant Biology 2022Quote: ... probed with stabilized streptavidin-horseradish peroxidase (HRP) and subsequently detected using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher). Membranes were imaged using a ChemiDoc XRS+ camera (BioRad).
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bioRxiv - Neuroscience 2023Quote: ... membranes were incubated in appropriate horseradish peroxidase secondary antibodies for 1hr and developed using SuperSignal west Femto ECL substrate (ThermoFisher) and scanned on an Azure 500 (Azure Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... His-tagged proteins were detected on film by chemiluminescence using a horseradish peroxidase-conjugated His antibody and SuperSignal West Femto substrate (ThermoFisher).
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bioRxiv - Evolutionary Biology 2022Quote: ... Membranes were washed with TBST three times for 5 mins and then incubated for 1 hour at room temperature with either donkey anti-rabbit or goat anti-mouse secondary antibodies conjugated to horseradish peroxidase (A16110 or 62–6520, respectively; Invitrogen) at 1:10,000 in TBST containing 5% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad #172-1011 or 172-1019, or ThermoFisher Scientific #31414) diluted in blocking buffer for 1 h at 22 °C ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-GAPDH, 1:5000 (CAB932Hu22, USCN) and horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat anti-mouse, 1:5000, G-21040, Invitrogen; Goat anti-rabbit ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibody binding was detected by a goat-anti-rabbit antibody conjugated to horseradish peroxidase (HRP) (1:2000 dilution; ThermoFisher). Signals were detected using a chemiluminescence substrate and the Azure c600 Imaging System (Azure Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... The following day the membranes were incubated with the secondary anti-mouse or anti-rabbit antibody coupled with horseradish peroxidase 1:30000 (Invitrogen) in 5% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then washed three times with TBS-T prior to incubating with a goat anti-rabbit horseradish peroxidase (HRP) conjugated antibody (Invitrogen). Chemiluminescence was detected using an enhanced chemiluminescence kit (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... the plates were incubated with horseradish-peroxidase conjugated goat anti-mouse IgG (diluted 1/5,000) secondary antibodies (ThermoFisher 62-6520) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... The horseradish peroxidase (HRP)-conjugated Protein A or HRP-conjugated rabbit anti-mouse secondary antibody for immunoblotting were from Invitrogen. The alpha smooth muscle actin (# ab7817) ...
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bioRxiv - Microbiology 2023Quote: ... β-actin expression was measured using an anti-human β-actin horseradish peroxidase-conjugated mAb (1:25000, Life Technologies, USA) as the loading control ...
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bioRxiv - Microbiology 2022Quote: ... The plates were washed three times with PBS with 0.05% Tween 20 and then incubated for 1 h at RT with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (1:5,000, Cat# 62-6520, ThermoFisher Scientific), IgG1 (1:5,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were subsequently rinsed and incubated for 1h at RT with the appropriate HorseRadish-Peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The visualisation of the signal was conducted using Immobilon™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were incubated at RT for 1 h with 1:1000 dilution of horseradish-peroxidase conjugated rabbit anti-guinea pig IgG secondary antibody (Invitrogen), or with 1:2500 dilution of horseradish-peroxidase conjugated donkey anti-mouse IgG secondary antibody (Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2023Quote: ... H2O2 content was measured by incubating lung homogenates with 100 μM/L of Amplex Red and horseradish peroxidase (Invitrogen, A22188) for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were subsequently rinsed and incubated for 1 h at RT with the appropriate horseradish-peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The signal was visualized using Immobilon® Forte Western HRP Substrate (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used are: goat anti-Mouse and goat anti-rabbit horseradish peroxidase (HRP)-conjugated antibodies (Invitrogen, Waltham, MA, USA) for Western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were incubated with horseradish peroxidase-conjugated (HRP-linked) secondary antibodies anti-rat IgG (ThermoFisher Scientific, 31470, 1:5000), anti-rabbit IgG (Promega ...
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bioRxiv - Genomics 2024Quote: ... Membranes were washed with TBST and incubated with mouse and rabbit secondary antibodies conjugated with horseradish peroxidase (Catalog# 31430, 31462, Invitrogen) for 2 h at room temperature followed by three washes in TBST ...
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bioRxiv - Biochemistry 2024Quote: ... The biotinylated inner membrane-associated protein MmdC was used as a loading control (48) and detected by a 1:4,000 dilution of streptavidin conjugated to horseradish peroxidase (Thermo Scientific).
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 1 h with a polyclonal Horseradish Peroxidase (HRP)-coupled anti-mouse IgG secondary antibody (Thermofisher Scientific) diluted 1:2000 in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... diluted in TBST at RT for 1h and then incubated for 1h at RT with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The visualization of the signal was conducted using Immobilon Forte Western HRP substrate (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3× 5min with 1×PBST and incubated with mouse or rabbit secondary antibodies conjugated with horseradish peroxidase (Invitrogen) for 1 h at room temperature followed 3 × 5 min wash by 1× PBST ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2 ul of a 1:20 dilution of ERCC RNA Spike-in mix (Invitrogen) was added to each sample.
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bioRxiv - Biochemistry 2022Quote: HEK293 wild type (ATCC, #CRL-1573) and HEK293 Flp-in T-REx (ThermoFisher, #R78007) wild type cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 Flp-In T-REx cells (or HEK293 T-REx; Thermo Fisher Scientific, R78007) and HEK293 cells [American Type Culture Collection (ATCC) ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...