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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and then incubated for 1 hour at room temperature with donkey anti-rabbit or goat anti-mouse secondary antibodies conjugated to horseradish peroxidase (Invitrogen, A16110, 62–6520) at 1:10,000 in TBST containing 5% (w/v ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... The membrane was washed once with washing buffer and incubated with a horseradish peroxidase-conjugated secondary antibody (31460, Thermo Fisher Scientific; 1:5000) for 1 h at RT ...
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bioRxiv - Physiology 2022Quote: ... Membranes were washed 3x with TBST and incubated with anti-mouse or anti-rabbit IgG horseradish peroxidase secondary antibody (A16017 or A16035, Thermofisher, 1:1,000-10,000) for 1 hr ...
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bioRxiv - Bioengineering 2022Quote: ... membranes were washed with three times with TBS-T for 15 min and then incubated with goat anti-rabbit horseradish peroxidase conjugate (1:4000; ThermoFisher Scientific, 65-6120) in blocking solution for 1 hr at room temperature (RT) ...
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bioRxiv - Cancer Biology 2022Quote: ... The blots were probed with the appropriate primary antibodies followed by goat anti-rabbit IgG or goat anti-mouse IgG conjugated to horseradish peroxidase and visualized using enhanced chemiluminescence substrate (SuperSignal™ West Dura Extended Duration Substrate #34076, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Blots were then washed three times with TBST (10 minutes/wash) and subsequently incubated with immunoglobulin-G labelled with horseradish peroxidase conjugated secondary anti-mouse antibody (1:5,000; Thermo Scientific™; Cat. #31432). Proteins were visualized by SuperSignal™ West Pico PLUS chemiluminescence reagent on a MyECL imager (Thermo Scientific™) ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was incubated for one hour with 1:10000 diluted horseradish peroxidase-coupled goat anti-mouse antibody (#62-6520, ThermoFisher Scientific, MA, USA) in PBS-T and washed again five times for 15 minutes with PBS-T ...
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bioRxiv - Cancer Biology 2021Quote: ... antibodies for 12 h at 4 °C followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1/500; Invitrogen, Thermo Fisher Scientific, Inc.). After a PBS wash ...
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bioRxiv - Genetics 2019Quote: ... Western blots were developed using anti-mouse or anti-rabbit secondary antibodies conjugated to horseradish peroxidase (anti-rabbit-HRP, Novex A16029 1:2000, anti-mouse-HRP, Invitrogen G21040 1:2000) and chemiluminescent substrate (BioRad Clarity Max 1705062) ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was exposed to an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) (PierceTM ECL Western; Thermo Scientific. Rockford, IL, USA). Bands were visualized and quantitated using the Chemi DocTM MP imaging system (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... The wells were washed with 1% BSA/LBB and incubated for one hour with L9393 (1:5000 dilution) in 3% BSA/LBB followed by incubation with Horseradish Peroxidase (HRP)-conjugated anti-rabbit IgG (Invitrogen, 1:5000 dilution) in 3% BSA/LBB for 30 minutes ...
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A new vulnerability to BET inhibition due to enhanced autophagy in BRCA2 deficient pancreatic cancerbioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated in primary antibody overnight with rocking at 4°C followed by incubation in appropriate secondary antibodies conjugated to horseradish peroxidase (1:5000) for 1 hr and detection by Super Signal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577). Primary antibodies used were ...
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bioRxiv - Bioengineering 2023Quote: ... membranes were washed three times with TBS-T and incubated with goat anti-rabbit horseradish peroxidase conjugate (1:4000; ThermoFisher Scientific, 65-6120) in blocking solution for 1 hr at room temperature (RT) ...
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bioRxiv - Molecular Biology 2023Quote: ... and developed with a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate (SuperSignal West Dura Extended Duration Substrate, Thermo Scientific™ #34076)
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times in 1X TBST and incubated with horseradish peroxidase-conjugated goat-anti-mouse or -rabbit secondary antibodies for one hour at room temperature (1:10,000, Invitrogen #31430, #31460, Rockford, IL). Membranes were subsequently washed three times in TBST ...
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bioRxiv - Immunology 2021Quote: ... and Spike-Alexa488 (Thermofisher Scientific, S32354) and Spike-APC (Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and Spike-APC (Thermofisher Scientific, S32362) or RBD-Alexa488 and RBD-APC ...
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bioRxiv - Developmental Biology 2021Quote: ... and ERCC spike-in RNA (Ambion) (1:1,000,000 dilution ...
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bioRxiv - Plant Biology 2021Quote: ... root and spike using Trizol (Invitrogen) method and Xcelgen plant RNA kit ...
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bioRxiv - Cell Biology 2022Quote: ... ERCC RNA spike-in (Thermo Scientific) were diluted into the RT mix (dilution 5×10-7) ...
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bioRxiv - Molecular Biology 2022Quote: ... ERCC RNA spike-in mix (Invitrogen) was added after shearing ...
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bioRxiv - Molecular Biology 2019Quote: ... 2µl of ERCC RNA spikes (ThermoFisher) were added to the eluate at a dilution of 1/25 000 for PFP and 1/5000 for PRP ...
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bioRxiv - Genomics 2019Quote: ... and ERCC spike-in control (ThermoFisher). Plates were centrifuged and placed on dry ice immediately before storage at −80°C.
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bioRxiv - Genomics 2021Quote: ... ERCC Spike-In (Thermofisher Scientific, 4456739) controls (0.5μl of mix 1 or mix 2 from 1:100 dilution ...
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bioRxiv - Immunology 2021Quote: ... and Spike-Alexa488 (Thermofisher Scientific, S32354) and Spike-APC (Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and Spike-APC (Thermofisher Scientific, S32362). Live CD3/CD8-CD14-CD19+IgM-IgD-IgG+Spike+Spike+ cells were sorted into individual wells containing RNase OUT (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... ERCC RNA Spike-in (Ambion, 4456740) was added as a technical control ...
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bioRxiv - Neuroscience 2022Quote: ... ERCC spike-ins (Life Technologies, 4456740) were added at a dilution of 1:80000 ...
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bioRxiv - Microbiology 2023Quote: ... ERCC spike-in controls (ThermoFisher 4456740) were added to 500-1000 ng of total RNA ...
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bioRxiv - Cell Biology 2023Quote: ... α-SPIKE (1:100, cat#703958, Invitrogen), α-LC3-B (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... we included ERCC Spike-ins (Ambion) at a 1:2500000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... ERCC-RNA-spike-in (ThermoFisher, 4456740) was added to every sample ...
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bioRxiv - Cell Biology 2024Quote: ... ERCC RNA Spike-in mix (Invitrogen) was added to all input samples per the manufacturer’s instructions (added for downstream normalization ...
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bioRxiv - Molecular Biology 2022Quote: ... treated with the S1 nuclease (Thermo Fisher) at 20 U/mL in S1 buffer (30 mM sodium acetate pH 4.6 ...
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bioRxiv - Cell Biology 2021Quote: ... Rabbit anti-Ki67 (Thermofisher RM-9106-S1), donkey anti-rabbit 647 (Jackson ImmunoResearch 711-605-152 ...
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bioRxiv - Molecular Biology 2023Quote: ... For S1 nuclease (ThermoFisher Scientific, Waltham, MA), reactions were performed with and without the addition of 1 mM ZnSO4 prior to equilibration at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL S1 Nuclease (Thermo Fisher, 18001016) at 37 °C for 5 min.The successful linearization of ecDNA was verified by verifying its sensitivity to exonucleaseATP-dependent DNase (Lucigen) ...
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bioRxiv - Physiology 2023Quote: ... 1:200 (Thermo Scientific, RM-9106-S1); anti-CHOP ...
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bioRxiv - Molecular Biology 2023Quote: ... treated with the S1 nuclease (ThermoFisher Scientific) at 20 U/mL in S1 buffer (30 mM sodium acetate pH 4.6 ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (FCS, Thermo Fisher Scientific), 100 U ml−1 penicillin ...
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bioRxiv - Molecular Biology 2021Quote: ... ELMSAN1 TRERF1 DKO HEK293 cells were generated from wild-type Flp-In T-REx HEK293 cells (Invitrogen, R78007) by the Center for Advanced Genome Engineering (CAGE ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Human embryonic kidney cell lines (HEK293) containing a Flp-In expression vector (HEK293 Flp-In) obtained from ThermoFisher Scientific were utilized in this study ...
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bioRxiv - Developmental Biology 2020Quote: ... diluted (1:10,000) in pre-incubation (PI) buffer (PBS, 0.1% Tween 20, 2% sheep serum, 2mg/ml BSA (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... overnight at 4°C and then for 2 hours with Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen 11201D). After 3 800uL washes in LiCl buffer (100mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... and 2 μL of each culture were spotted onto a TSA plate containing 5% sheep’s blood (Thermo Fisher, R060312). The plates were incubated at 30°C for 72 h and imaged above a white light ...
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Dose-dependent effects of histone methyltransferase NSD2 on site-specific double-strand break repairbioRxiv - Cell Biology 2023Quote: ... 2 μg of anti-NSD2 antibody (ab75359) and 20 μl of Dynabeads M-280 sheep anti-mouse IgG (Invitrogen) were mixed in immunoprecipitation dilution buffer and incubated for 6 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of hK14-LaNto31-T2A-mCherry or LaNt-α31-pSec-Tag and 2 μl Lipofectamine 2000 (Thermo Fisher Scientific) were used ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... filtered through a 70 µm cell strainer, washed with FACS buffer (PBS supplemented with 2% FCS, 2 mM EDTA and 0.01% Sodium Azide (all Gibco)) and maintained at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were spiked-in with the ERCC spike-in controls (2 μL/sample, diluted 1:100, Invitrogen #4456740), treated with on-column DNase digestion according to manufacturer’s specifications (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated spike protein was purified using a 40K MWCO 2 mL Pierce Zeba™ desalting column (Thermo Fisher Scientific ...