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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Detection was performed with horseradish peroxidase-conjugated secondary antibodies and SuperSignal West Femto chemiluminescent substrate (ThermoFisher, cat. # 34094).
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bioRxiv - Microbiology 2020Quote: ... Signal development was accomplished using a 1:200 dilution of streptavidin-horseradish peroxidase (Invitrogen; Cat. No. 43-4323) for 30 minutes followed by incubation with Nova Red chromagen solution (Vector ...
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bioRxiv - Cell Biology 2021Quote: ... conjugated to horseradish peroxidase at 1:5,000 before application of SuperSignal West Femto enhanced chemiluminescent (ECL) substrate (ThermoFisher) and subsequent imaging with iBright CL1000 Imaging System (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody diluted 1:2000 (Thermo Fisher Scientific Inc., G-21234). Membranes were washed with PBST (0.1% Tween-20 ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies were then detected using horseradish peroxidase (HRP) conjugated antibodies (anti-mouse or anti-rabbit IgG, Invitrogen) at 1:1,000 dilution in 5% non-fat dry milk 0.1% Tween-20 in TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... After washing and re-probing with a horseradish peroxidase-conjugated secondary antibody (Thermo Fisher Scientific, cat no: A11077), bands were visualized with the iBright CL1500 Imaging system (Applied Biosystems).
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bioRxiv - Neuroscience 2022Quote: Horseradish peroxidase-catalyzed chemiluminescence from SuperSignal West Dura Extended Duration HRP Substrate (catalog number 37071, Thermo Fisher Scientific) was detected on radiography film and band intensities were quantified on ImageJ software version 1.53e.
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bioRxiv - Bioengineering 2022Quote: ... Blots were then incubated with the appropriate mouse or rabbit horseradish peroxidase conjugated secondary antibody (Thermo Fisher Scientific) for two hours at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Visualization occurs through an enzymatic reaction using streptavidin horseradish peroxidase (HRP) (Cat. No. 21130, Thermo Fisher, Waltham, MA). Chicken PRL antibodies have been successfully used in ELISA to measure prolactin from other avian species ...
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bioRxiv - Microbiology 2023Quote: ... ab6556)] were used at 1:1,000 in blocking buffer and detected with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5,000; ThermoFisher). Membranes were washed in TBST followed by addition of enhanced chemiluminescent (ECL ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies conjugated with horseradish peroxidase and SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific, Waltham, MA) were used to detect proteins of interest ...
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bioRxiv - Immunology 2023Quote: ... the ELISA plates were incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (1:5000, Invitrogen) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary goat anti-rabbit or goat anti-mouse antibodies conjugated to horseradish peroxidase (HRP) (Thermo Fisher Scientific, Invitrogen) were used for detection ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary goat anti-rabbit or goat anti-mouse antibodies conjugated to horseradish peroxidase (HRP) (Thermo Fisher Scientific, Invitrogen) were used for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... Horseradish peroxidase (HRP)–conjugated secondary IgG antibodies (Invitrogen, #31470; Jackson ImmunoResearch, #111-035-144; Invitrogen, #A18769; 1:5000) were used together with the Clarity Max™ Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... All primary antibody incubations were followed by incubation with secondary horseradish peroxidase (HRP)– conjugated antibody (Pierce, Thermo Fisher) in 5% milk and 0.1% Tween 20 in TBS and visualized using Super Signal West Pico or Femto Chemiluminescent Substrate (Pierce ...
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bioRxiv - Plant Biology 2024Quote: ... followed by incubation in 1:10,000 dilutions of horseradish peroxidase-conjugated rabbit anti-goat (Thermo Fisher Scientific, 811620) or goat anti-rabbit (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were detected by horseradish peroxidase-conjugated secondary antibody using SuperSignal West Pico PLUS chemiluminescent substrate (ThermoFisher Scientific) and imaged with an Invitrogen iBright FL1500 imaging system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed and then incubated with PierceTM horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, Inc., USA) and incubated with Super Signal West Femto or Pico chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... was used at a 1:1,000 dilution with horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody (Thermo Fisher) at a 1:10,000 dilution.
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bioRxiv - Immunology 2024Quote: ... was diluted 1:500 and the secondary antibody goat anti-mouse conjugated to horseradish peroxidase (ThermoFisher #62-6720) was diluted 1:5000.
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bioRxiv - Developmental Biology 2022Quote: ... or with Pierce™ Glycoprotein Staining Kit (Thermo Scientific) after separation.
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bioRxiv - Microbiology 2023Quote: 293T cells (2 × 106) were transfected with 2 μg spike expression vector by lipofection using lipofectamine 2000 (Invitrogen). One day post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... and S1 nuclease (Invitrogen). Total RNA was extracted from the pulverized samples using the TRIzol Plus RNA Purification Kit (Invitrogen) ...
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bioRxiv - Genetics 2022Quote: ... S1 nuclease (ThermoFisher #18001016) at 20 U/ml in S1 nuclease buffer and 50 mM NaCl was added to one of the two slides and incubated for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: HEK293 and commercially available Flp-In T-REx HEK293 cells (Invitrogen; R78007) were cultured in DMEM (GIBCO ...
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bioRxiv - Immunology 2020Quote: ... Washing medium consisted of 2% FCS and 2% P/S in RPMI 1640 (Gibco). FACS buffer contained 2% FCS and 2mM EDTA in PBS ...
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bioRxiv - Immunology 2020Quote: ... 2 mM L-Glutamine and 50 µm 2-Mercaptoethanol) with 10% FCS (all Gibco). Colon and caecal tissue were digested in an enzyme cocktail of 0.45 mg/ml collagenase V (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with foetal calf serum (FCS; 2 %; Thermo Fisher Scientific) and EDTA (2 mM ...
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bioRxiv - Immunology 2020Quote: ... the supernatant was discarded and 1mL 2% FCS media (Gibco) + 1mL B11F diluted in 2% FCS was added to the plate ...
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bioRxiv - Immunology 2020Quote: ... and 2% heat-inactivated fetal calf serum (FCS; Gibco A38401) for 20 minutes ...
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bioRxiv - Immunology 2024Quote: ... containing 2mM EDTA (Thermo) and 2% FCS (Gibco or Omega) with a 23 gauge needle into the peritoneal cavity and then retrieving the solution back out with the same syringe and a 21 gauge needle ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by a donkey anti-sheep IgG coupled to horse radish peroxidase (R&D, MN) and developed with an ECL Western blotting kit (Thermo Scientific, NJ). The intensities of the cytochrome c bands were normalized to that of constitutively expressed β-actin and expressed as relative intensity ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells (ThermoFisher Scientific) were cultured in DMEM/F-12 ...
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bioRxiv - Bioengineering 2022Quote: ... FreeStyle HEK293 cells (ThermoFisher) were used for recombinant S protein production ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293 FT cells (ThermoFisher) were co-transfected with plasmids pCMV-dR8.2 dvpr (gag-pol ...
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bioRxiv - Synthetic Biology 2019Quote: HEK293 cells (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293-FT (Invitrogen # R70007) cells were grown in Dulbecco’s Modified Eagle Medium (ThermoFisher #11965118 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 (Thermo Fisher Scientific) were cultured in DMEM (Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Developmental Biology 2022Quote: ... and 3.5 µg of pCAGGS SARS CoV-2 S Glycoprotein expression vector DNA (NR52310, BEI, USA) in a total of 10 ml of Opti-MEM media (Invitrogen). The day following transfection the media was changed to DMEM10 complete media and samples were placed at 37 °C and 5% CO2 for 48 h ...
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bioRxiv - Developmental Biology 2023Quote: ... and ERCC RNA ExFold RNA Spike-In Mix 1 and 2 (Thermo Fisher Scientific) were added to wild-type and Meioc-null samples ...
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bioRxiv - Cell Biology 2022Quote: ... 2 µg of antibodies against the V5-tag (Invitrogen, R960-25), the c-Myc tag (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... 2 µg/ml Heparin) and secondary antibodies against sheep (1:500, Invitrogen, A21436 or A21448) and/or rabbit (1:500 ...
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bioRxiv - Pathology 2019Quote: ... followed by Horseradish Peroxidase (HRP) conjugated goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody (Invitrogen, USA) 1/4000 diluted in assay buffer ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were then washed and incubated with anti-mouse-horseradish peroxidase (1:5000 dilution in PBS; Thermo Fisher Scientific) and visualized with 1-Step Ultra-TMB (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... Primary antibodies against GAPDH (MA5-15738) and horseradish peroxidase-conjugated secondary antibodies (31460 and 31430) were purchased from Invitrogen. Images were quantified using ImageJ (version 1.51e ...
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bioRxiv - Biophysics 2021Quote: ... Then membranes were incubated with corresponding horseradish-peroxidase (HRP) labeled goat anti-mouse secondary antibody (31444; Thermo Fisher Scientific) or horseradish-peroxidase (HRP ...
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bioRxiv - Plant Biology 2021Quote: ... and horseradish peroxidase (HRP)-coupled goat anti-mouse IgG was used as a secondary antibody horseradish peroxidase (0.5 μg/mL for 1 hr at 21 °C; Invitrogen). Ubiquitin immunoblotting was performed with a rabbit anti-UBQ polyclonal antibody (0.1 μg/mL for 1 hr at 21 °C ...
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bioRxiv - Neuroscience 2021Quote: ... 1:1,000] and secondary antibodies (horseradish peroxidase-conjugated anti-rabbit antibody [Cytiva, 1:2,500], Alexa Fluor 488-conjugated anti-rabbit antibody [Thermofisher, 1:2,500] ...