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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... or BA.5 (2 μg/mL) spike proteins were coated onto 96-well Maxisorp plates (ThermoFisher, Cat. #439454) in 50 μL of 50 mM Na2CO3 pH 9.6 overnight at 4°C ...
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bioRxiv - Genomics 2024Quote: ... ERCC Mix 1 or Mix 2 spike-ins were randomly added to each sample (Thermo Fisher, catalog #4456740) before pooling and sequencing on Illumina NovaSeq S4 to an average depth of 50 million paired-end reads per sample as previously described95 ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed for 2 genes of interest and 2 internal controls (Suppl Figure S1) using Taqman® gene expression assays (Thermo Fisher, Foster City CA) and Taqman® Fast Universal Master Mix (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... V5 tag to detect CBUA0028 or the Xpress tag (Invitrogen) to detect CBU0665.
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bioRxiv - Microbiology 2022Quote: ... V5 tag to detect CBUA0028 or the Xpress tag (Invitrogen) to detect CBU0665.
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bioRxiv - Cell Biology 2020Quote: HEK293 Flip-In TREx cells (Thermo Fisher) were cultured at 37°C with 5% CO2 in DMEM (Thermo-Fisher ...
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bioRxiv - Biochemistry 2020Quote: HEK293-F cells (FreeStyle™, ThermoFisher #R79007) were maintained in suspension culture according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293T or HEK293 GnTI- cells (Thermo Fisher) were incubated with 200 nM (monomer concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 Flp-In T-REx 293 (Invitrogen), was grown at 37°C in DMEM high glucose supplemented with 5% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2022Quote: HEK293-Flp-In T-Rex cells (Invitrogen) were cultured in high glucose DMEM containing 10% FBS at 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells were cultured in DMEM (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2021Quote: HEK293 cells grown in DMEM media (Gibco) supplied with 10%FBS and 2mM L-Glutamine were seeded onto coverslips in 24 well plates and allowed to adhere and grow overnight at 37°C incubator supplied with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293-FT cells were purchased from Invitrogen. U87MG cells were Purchased from the ATCC and cultured as previously described (Sabag et al. ...
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bioRxiv - Immunology 2020Quote: ... HEK293 cells was cultured in DMEM (Invitrogen) supplemented with 10% FBS at 37 °C and 5% CO2 environment ...
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bioRxiv - Cancer Biology 2022Quote: ... Flp-In T-REx HEK293 cells (ThermoFisher) were cultured in DMEM (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: Flp-In 293 cell line (HEK293; ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were maintained in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Genetics 2023Quote: HEK293 cells (Thermo Fisher, Waltham, MA, USA) were cultured at 37°C in Dulbecco’s Modified Eagle’s Medium containing high levels of glucose (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in DMEM (Gibco) containing penicillin (100 U/mL ...
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bioRxiv - Microbiology 2024Quote: HEK293 cells were maintained in DMEM (Invitrogen) supplemented with 10% FBS (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney (HEK293, Thermo Fisher Scientific) cells ...
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bioRxiv - Genetics 2024Quote: ... HEK293 cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were grown in DMEM (Gibco) medium with 10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: Flp-InTM T-RexTM HEK293 (Thermo Fisher), Flp-InTM T-RexTM HeLa (gift from S ...
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bioRxiv - Immunology 2021Quote: ... biotinylated SARS-2 and Rs4081 RBDs were incubated with streptavidin-APC (eBioscience™) and streptavidin-PE (ThermoFisher), respectively ...
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bioRxiv - Biochemistry 2020Quote: ... mature S from SARS-CoV-2 (GenBank Acc. No. YP_009724390.1; a.a. V16-T1273) into the NheI-XhoI sites of pCEP4 (Invitrogen) with an N-terminal ...
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bioRxiv - Microbiology 2023Quote: ... and COV-2-2196 were made by transfecting 3.60 × 108 293-Freestyle cells (ThermoFisher Scientific, Waltman, MA) in 240 mL FreeStyle 293 media (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Expression levels were correlated to eight synthetic spike-in RNAs (ArrayControl RNA spikes Ambion, cat. no. AM1780) (60) ...
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bioRxiv - Neuroscience 2024Quote: ... DRG neurons or HEK293 cells were incubated with 5 μM Fura-2 AM and 0.2% Pluronic (Thermo Fisher Scientific) (prepared from a 200 mg/ml stock solution in DMSO ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 2% v/v heat-inactivated FCS and sodium bicarbonate (Gibco 25080-060). Diluted compounds were then mixed with EGFP-expressing Vero E6 cells at 25,000 cells/well in 96-well plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2021Quote: ... HA-tag (Invitrogen), Flag-tag (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were then washed in PBST and incubated with secondary antibody anti-mouse IgG (H+L) coupled to horseradish peroxidase (1/20000; ThermoFisher Scientific Cat# 32230, RRID:AB_1965958). Proteins were revealed by SuperSignal™ West Pico Chemiluminescent Substrate (ThermoFisher Scientific Cat# 34580 ...
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bioRxiv - Biochemistry 2019Quote: ... the membrane was washed 3x with TBST and then incubated with either horseradish peroxidase-coupled secondary anti-mouse antibody (1:2000, Invitrogen # 62-6520, RRID: AB_2533947) or anti-rabbit antibody (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibodies were used to detect the primary antibodies (Thermo Scientific, Waltham, MA, USA). After addition of a chemiluminescent HRP substrate (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were washed three times with TBST and then were incubated for 1h with a horseradish peroxidase labeled immunoglobulin G (IgG-HRP) secondary antibody (Thermo Fisher Scientific, no. 31430) at 1:7500 dilution ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... The anti-FLAG antibody was subsequently detected with goat anti-mouse IgG (H+L) conjugated to horseradish peroxidase (HRP) to facilitate chemiluminescent detection (Invitrogen 32430, 1:1,000 dilution). Chemiluminescent signal was produced with Pierce ECL substrate (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed with PBST and incubated with goat anti-mouse IgG (H+L) secondary antibody conjugated with horseradish peroxidase (Thermo Fisher, 31430, 1:10,000) on a shaker at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were incubated overnight at 4°C and then washed three times with 0.3 % PBST followed by incubation with a horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (Alexa Fluor Plus™ 647 goat anti-rabbit IgG, ThermoFisher, USA; 1:5000) for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... antibodies for 12 h at 4 °C followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1/500; Invitrogen, Thermo Fisher Scientific, Inc.). After a PBS wash ...
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bioRxiv - Cancer Biology 2022Quote: Membranes were incubated for one hour in secondary antibody diluted in 5% NFDM TBST at a concentration of 80 ng/ml: Goat anti-rabbit or goat anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (31460 and 31460 respectively, Thermo Fisher Scientific, Waltham, MA). Clarity Western ECL (Biorad ...
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bioRxiv - Neuroscience 2023Quote: ... Immunoreactive bands were visualized with secondary goat-anti mouse or goat anti-rabbit antibodies conjugated with horseradish peroxidase and with an enhanced chemiluminescence system (Thermo Fisher Scientific, Schwerte, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... The next day blots were incubated for 1 hour at room temperature with horseradish peroxidase (HRP)-conjugated secondary antibodies and were developed using SuperSignal™ West Femto Maximum Sensitivity Substrate Femto (Thermo Scientific™, 34096).
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bioRxiv - Neuroscience 2024Quote: ... This was followed by a 1 h room temperature incubation with a horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000; Thermo Fisher Scientific, Waltham, MA). The membranes were developed using Clarity Western ECL (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... In the case of sheep polyclonal anti-H2A.Z (acetyl K4+K7+K11, ab18262) Alexa fluor 633 (A633) conjugated goat anti-sheep IgG (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Purifications were carried out from 150 μl of the whole-cell extract (2 mg protein) per experiment using 37 µl of Dynabeads M-280 sheep anti-rabbit IgG (Invitrogen) and 2 µl of the anti-LytA antibody (Biomedal) ...
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bioRxiv - Bioengineering 2024Quote: ... at a 1:200 dilution for 2 h at room temperature (RT) followed by donkey anti-sheep Alexa Fluor 647 secondary antibody (Invitrogen) at a 1:400 dilution and donkey anti-mouse Alexa Fluor 555 antibody (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... GST-tag and 3xFLAG-tag fusions were achieved using pDEST27 (Invitrogen) and pCI-neo-3xFLAG vector ...
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bioRxiv - Molecular Biology 2023Quote: ... dye-labeled DNA template (Figure 1A and Table S1) was prepared by PCR from pUC18-G5CYC1 G- (Table S1) with Platinum Taq DNA polymerase (Invitrogen) and primers Biotin-universal and AF488-M13 rev2 (Table S1) ...
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bioRxiv - Immunology 2023Quote: ... Live CD3/CD8-CD14-CD19+IgM-IgD-IgG+S1+S1+ cells were sorted using a BD FACS Melody into individual wells containing RNase OUT (Invitrogen), First Strand SuperScript III buffer ...
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bioRxiv - Molecular Biology 2020Quote: The remaining RNA was combined into four final bsRNA-seq fractions (Figure 1) and 10μg RNA was spiked with ERCC Spike-in Mix 2 (Ambion) according to manufacturer’s instructions ...