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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... immuno-reactive proteins were blotted with anti-rabbit or goat horseradish peroxidase-labeled secondary antibodies for 1h at room temperature and revealed by enhanced chemiluminescence reagent (SuperSignal West Femto, Thermo Scientific), visualized using ChemiDoc MP Imaging System and data analyzed using the ImageLab 4.2 version software (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2019Quote: ... all used at 1:10,000 dilution in TBS with 0.1% v/v Tween-20 (TBS-T) and horseradish peroxidase-conjugated rat IgG secondary antibody (#31470, Thermo Fisher Scientific) used at 1:10,000 dilution in 5% non-fat dry milk dissolved in TBS-T.
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bioRxiv - Microbiology 2019Quote: ... The blots were developed using horseradish peroxidase-conjugated secondary antibodies in conjunction with the Super Signal West Dura chemiluminescence detection system (Thermo Fisher).
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bioRxiv - Developmental Biology 2020Quote: ... The chemiluminescent detection of the horseradish peroxidase on the secondary antibodies was detected by the Supersignal West Dura kit (Thermo Scientific). Images and of western blots were acquired on a ChemiDoc MP system (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... and bound antibodies were detected by incubating the plates with horseradish peroxidase-conjugated goat anti-mouse IgG or IgM (Invitrogen, UK), diluted in blocking buffer ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were incubated with the antibodies indicated above and visualized with horseradish peroxidase–conjugated secondary antibodies using the ECL Western blotting system (Thermo Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated overnight in a 1:2000 dilution of horseradish peroxidase-linked anti-rabbit IgG secondary antibody (A24537 Life Technologies) in block ...
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bioRxiv - Zoology 2021Quote: ... The membrane was incubated with a streptavidin-horseradish peroxidase conjugate and was detected using the ECL Western Blot Kit (Thermo Fisher).
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bioRxiv - Immunology 2021Quote: ... Cat#PA1-8463) or goat-anti-monkey IgG (H+L, Cat#A18811) secondary antibody conjugated to horseradish peroxidase (HRP, Thermo Fisher) in blocking buffer at 1:10,000 or 1:4,000 dilution ...
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bioRxiv - Immunology 2020Quote: ... the bound antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse IgG or anti-Macaca fascicularis IgG antibody (1:5,000, Thermo Fisher Scientific) for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed three times for 10 mins with TTBS before incubating with goat anti-rabbit-horseradish peroxidase secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Foci development was stopped by fixation with 4% formaldehyde and foci were then stained using a mouse-anti-NS1 antibody (1A5) (gift from Jacob Schlesinger, Rochester University) and a horseradish peroxidase (HRP) conjugated secondary anti-mouse antibody (ThermoFisher 31430). The foci were visualized by diaminobenzidine (DAB ...
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bioRxiv - Biophysics 2024Quote: ... in TBS-T in 5% Bovine Serum Albumin (BSA) and probed with horseradish peroxidase-conjugated secondary antibodies and developed with SuperSignal West Pico Plus chemiluminescent substrate (ThermoFisher #34580) and imaged using an Amersham Imager 600.
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bioRxiv - Cell Biology 2024Quote: ... Secondary goat anti-rabbit (#31460) or goat anti-mouse (#31430) antibodies conjugated to horseradish peroxidase (HRP) (Thermo Scientific; 1:5,000 dilution) were used for detection ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used are: ECL anti-mouse immunoglobulin G (IgG) horseradish peroxidase linked whole antibody (1/10 000; Fisher Scientific; NXA931V) for anti-MAP1B ...
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bioRxiv - Immunology 2024Quote: ... The signal was further detected using secondary antibody of goat anti-rabbit IgG conjugated with horseradish peroxidase (Thermo Scientific, Waltham, MA). The band signal was developed with Immobilon™ Western Chemiluminescent HRP substrate (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated overnight at 4C with primary antibodies in TBST in 5% Bovine Serum Albumin (BSA) and probed with horseradish peroxidase-conjugated secondary antibodies and developed with SuperSignal West Pico Plus chemiluminescent substrate (ThermoFIsher #34580) and imaged using an Amersham Imager 600 ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used to amplify the signal included the Anti-Goat IgG HORSERADISH Peroxidase (HRP) Conjugated (Donkey Polyclonal, Life Technologies, A16005), the Anti-Rabbit IgG ...
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bioRxiv - Microbiology 2023Quote: ... The plates were then washed and incubated for 1 hour with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Bioengineering 2023Quote: ... Primary antibodies were detected by a horseradish peroxidase (HRP)-conjugated anti-rabbit antibody using an ECL kit (Thermo Fisher Scientific, US) and imaged using ChemiDoc TM Touch Imaging System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and goat anti-mouse IgG (G-21040) secondary antibodies conjugated to horseradish peroxidase (HRP) were used at 1:5,000 (ThermoFisher Scientific Invitrogen). SuperSignal West Pico Chemiluminescent Substrate was used to detect HRP on immunoblots per manufacturer instructions (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Samples were washed and then incubated with a horseradish peroxidase-conjugated antibody reactive to mouse IgG1 (clone: PA1-74421; ThermoFisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Signal detection was done using anti-goat secondary antibodies conjugated to horseradish peroxidase (0.375 µg/ml, Jackson Immuno-Research) in combination with enhanced chemiluminescence solution (Thermo-Fisher Scientific, WP20005). Visualization was performed on a ChemiDoc MP imaging system (Biorad).
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bioRxiv - Bioengineering 2023Quote: ... Horseradish peroxidase-conjugated (#TL280988) and Alexa Fluor 647 (#A21235) conjugated goat anti-mouse IgG secondary antibodies were purchased from Thermo Fisher. Type F immersion oil was purchased from Leica Microsystems (Deerfield ...
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bioRxiv - Genetics 2023Quote: ... The membrane was incubated with streptavidin-conjugated horseradish peroxidase and then with reagents of the Chemiluminescent Nucleic Acid Detection Module Kit (ThermoFisher, 89880). The membrane was then scanned with the use of a ChemiDoc XRS+ System (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... the blots were incubated with horseradish peroxidase-conjugated secondary antibody and developed using chemiluminescence (ECL) method (Thermo Fisher Scientific, Cat. # 32106). A list of antibodies used were included in Supplementary Table 2.
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bioRxiv - Cell Biology 2023Quote: ... Subsequent incubation with horseradish peroxidase-conjugated secondary antibodies at 1:40,000 dilution was followed by detection with SuperSignal West Pico Plus (ThermoFisher Scientific #34580) or Immobilon ECL Ultra Western HRP Substrate (Sigma #WBULS0500) ...
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bioRxiv - Genetics 2023Quote: Secondary antibody solution was then removed and membranes were washed three times for 5 minutes in TBST and then incubated in enhanced chemiluminescence horseradish peroxidase (ThermoFisher Scientific) for 2 minutes before imaging on a Amersham Imager 680 machine (General Electric).
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bioRxiv - Genomics 2023Quote: ... Blots were incubated with an appropriate secondary antibody coupled to horseradish peroxidase at room temperature for 1 hour and reacted with ECL reagents per the manufacturer’s (Thermo Scientific, USA) suggestion and detected by Biorad ChemiDoc MP Imaging System.
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bioRxiv - Physiology 2023Quote: ... Samples were visualized using horseradish peroxidase coupled to appropriate secondary antibodies with enhancement by an ECL detection kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... The membranes were then probed with horseradish peroxidase-conjugated secondary antibodies and developed with enhanced chemiluminescence western blotting substrate (Thermo Scientific). Blots were visualized with detection system ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and then incubated with Horseradish peroxidase secondary antibodies against the last antibody: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen ® ...
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bioRxiv - Cell Biology 2023Quote: ... followed by PBS-Tween washes and secondary antibody incubation (2h at room temperature): Horseradish peroxidase secondary antibodies: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen® ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies included horseradish-peroxidase-conjugated anti-mouse or anti-rabbit IgG (1:2000 or 1:5000; ThermoFisher, 31430 or 31460). Proteins were detected by ECL chemiluminescence detection kits (ThermoFisher ...
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bioRxiv - Plant Biology 2024Quote: ... and horseradish peroxidase-labeled goat anti-rat as the secondary antibody at a dilution of 1:5000 (Invitrogen, catalog no. 31470).
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bioRxiv - Genetics 2024Quote: ... the membranes were washed three times for 10 minutes each with TBST and the appropriate Horseradish peroxidase (HRP) coupled secondary antibody (1:10,000) was added (Thermo Fisher Scientific) in TBST containing 5% non-fat milk and incubated for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... the spike-in culture was aliquoted in a 1:2 ratio with RNAlater™ solution (Invitrogen) on ice and stored at -80 °C.
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bioRxiv - Genomics 2020Quote: ... 2 µl External RNA Control Consortium (ERCC) spike-in controls (ThermoFisher Scientific, Waltham, MA, USA, 4456740), 2 µl Library Prep Control (LP ...
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bioRxiv - Cancer Biology 2020Quote: ... each containing an equal amount of ERCC ExFold RNA Spike-In Mix 2 (Ambion, ref. 4456739), was used ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of the purified RNA was added of ERCC RNA Spike-In Mix 2 (ThermoFisher Scientific #4456740 ...
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bioRxiv - Molecular Biology 2023Quote: ... Inputs were spiked with 2 µL of 1:10 diluted ERCC Spike-in mix (ThermoFisher #4456740) and cleaned using a Zymo RNA cleanup kit (Zymo Research #R1016 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% v/v (FCS) and 2 mM L-glutamine (Gibco). Where indicated ...
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bioRxiv - Immunology 2023Quote: ... Samples were stained in FACS buffer (PBS containing 2% FCS (Thermo Fisher) and 2 mM EDTA (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-sheep (Thermofisher, SA5-10060). Images were obtained by the Odyssey CLx imaging system and analyzed by Image Studio 5.2.5 (LI-COR).
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bioRxiv - Neuroscience 2022Quote: ... donkey anti-sheep (Life Technologies, A11016). For anti-BrdU immunohistochemistry (IHC) ...
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bioRxiv - Microbiology 2021Quote: ... and 5% sheep blood (Life Technologies) and then covered with a cover glass ...
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bioRxiv - Microbiology 2020Quote: ... 5% sheep blood (Thermo Fisher Scientific) and/or 5 μg/ml hemin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... anti-sheep (A11015, Thermo Fisher Scientific), Alexa555-conjugated anti-mouse (A21422 ...
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bioRxiv - Microbiology 2022Quote: ... and donkey anti-sheep (A21448, ThermoFisher).
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bioRxiv - Neuroscience 2024Quote: ... sheep anti-parvalbumin (PA5-47693, Invitrogen), goat anti-CD31 (AF3628 ...