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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Wells were then incubated with a secondary goat anti-human IgG labeled with horseradish peroxidase (HRP) (1:5000 Invitrogen) TMB substrate (SIGMA) ...
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bioRxiv - Plant Biology 2022Quote: ... and horseradish peroxidase (HRP)-labelled secondary antibodies for luminescence detection (SuperSignal™ West Femto Maximum Sensitivity Substrate, ThermoFisher, France).
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bioRxiv - Plant Biology 2022Quote: ... and G when used at 1:2,000 dilution) and anti-GFP conjugated with horseradish peroxidase (Invitrogen, 1:2,000 dilution) with gentle shaking overnight at 4°C to detect MMV proteins and GFP respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies conjugated with horseradish peroxidase were used at dilution of 1:3000 (anti-rabbit HRP; 32260, Thermo Scientific). Protein was detected by a chemiluminescent reaction (WesternBright ECL ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with either horseradish peroxidase (HRP)-conjugated anti-c-myc antibody (1:5,000) (Thermo Fisher Scientific) or HRP-conjugated anti-GFP antibody (1:5,000 ...
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bioRxiv - Microbiology 2023Quote: ... and goat anti-mouse IgG (G-21040) secondary antibodies conjugated to horseradish peroxidase (HRP) were used at 1:5,000 (ThermoFisher Scientific Invitrogen). SuperSignal West Pico Chemiluminescent Substrate was used to detect HRP on immunoblots per manufacturer instructions (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... was diluted to 1:10,000 followed by a secondary goat α-mouse antibody conjugated to horseradish peroxidase (Thermo Scientific) at a 1:3,000 dilution to determine complement activation by measurement of C4b deposition ...
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bioRxiv - Cell Biology 2023Quote: ... and secondary antibodies (i.e., anti-mouse, 1:3000 and anti-rabbit IgG, 1:4000 conjugated to horseradish peroxidase; Invitrogen 31432/AB_228302 and 31460/AB_228341 ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection of horseradish peroxidase-conjugated secondary antibodies was performed with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) under ChemiDoc touch imaging system (Bio-Rad).
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibodies used were: goat anti-mouse and goat anti-rabbit horseradish peroxidase (HRP)-conjugated antibodies (Thermo Fisher Scientific), donkey anti-goat and anti-rabbit IgG Alexa Fluor 594/ 488 conjugates (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... the sections were further incubated in the cross-adsorbed horseradish peroxidase-labeled goat anti-rabbit secondary antibody (Invitrogen, USA) diluted 1:500 in the blocking solution for 90 minutes in RT ...
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bioRxiv - Immunology 2023Quote: ... or horseradish peroxidase (HRP)-conjugated rabbit anti-guinea pig IgG (H+L) antibody (1:500; Invitrogen, Cat. PA1-28597) in blocking buffer for 1-1.5 hours at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... The following primary antibodies and dilutions were used overnight at 4 °C in PBST with 1% milk: mouse anti- V5 conjugated to horseradish peroxidase (HRP; 1:5,000; Invitrogen), mouse anti-V5 (1:5,000 ...
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bioRxiv - Microbiology 2023Quote: ... The binding of the primary antibodies was detected using horseradish peroxidase-conjugated anti-rabbit immunoglobulins (Thermo Scientific, Waltham, MA) at a 1:3000 dilution ...
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bioRxiv - Biophysics 2023Quote: ... The plate was then washed and incubated with horseradish peroxidase-conjugated anti- human IgG (InvitrogenTM, ThermoFisher Scientific, Waltham, MA) at a dilution of 1:2000 for 2h at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) (1:10,000 NA934 or NA931, Amersham) and exposed to ECL substrate (SuperSignal West Femto, ThermoFisher) for chemioluminiscence detection ...
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bioRxiv - Microbiology 2024Quote: ... The blots were washed and hybridized with anti-rabbit IgG horseradish peroxidase-linked antibodies (1:10000 dilution, ThermoFisher, 31460) and anti-mouse IgG HRP-linked whole Ab (1:10000 dilution ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times with TTBS before being probed for 60 min with a 1:10,000 dilution of a polyclonal goat anti-rabbit horseradish peroxidase-conjugated secondary antibody (Invitrogen). Development was carried out using the ECL Western blot substrate kit according to the manufacturer’s instructions (Millipore) ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris-produced LysM protein solution was tested using a protein polyacrylamide gel followed by CBB and glycoprotein staining with the Pierce Glycoprotein Staining Kit (Thermo Fisher Scientific, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The coding sequence of MHV-S Hemagglutinin Esterase inactive (S45A) (1218bp) fused to human IgG-Fc followed by a 6xHis tag in the mammalian expression vector pcDNA3.1(-) (Invitrogen) was kindly provided by Colin Parrish (MHV-S-HE_Fc_His6 ...
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bioRxiv - Immunology 2020Quote: ... Pro-Q™ Emerald 300 glycoprotein gel stain kit (Invitrogen) was used to detect the presence of sugar in Bl-Eng2 before and after deglycosylation ...
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bioRxiv - Cell Biology 2020Quote: Live HUVECs and HEK293 cells were stained with Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (ThermoFisher) at 10 μM (HUVECs ...
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bioRxiv - Immunology 2021Quote: ... 2 × 106 CHO-hCD4 or HEK293-hCD4 were non-enzymatically detached using dissociation buffer (Gibco) and suspended in 5% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... Monoclonal peroxidase-conjugated antibodies against the His6-tag (HisProbe™-HRP Conjugate) were purchased from Thermo Scientific (Langenselbold, Germany) and from Roche (Grenzach-Whylen ...
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bioRxiv - Developmental Biology 2022Quote: ... 30 µg of pCAGGS SARS CoV-2 S Glycoprotein expression vector DNA (NR52310, BEI, USA) in a total of 10 mL of Opti-MEM media (Invitrogen, USA). One day after transfection the media was removed ...
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bioRxiv - Developmental Biology 2019Quote: ... ERCC spike-in (Invitrogen) were added in the lysis buffer (1:1000000 ...
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bioRxiv - Cancer Biology 2024Quote: ... ERCC spike-in (Invitrogen) was added at a ratio of 2uL of spike-in per 1ug of RNA.
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bioRxiv - Immunology 2023Quote: RAVV GPΔMuc fused with Strep II and Avi tags was expressed in HEK293S GNTI−/− cells (ATCC, Manassas, VA) using 293fectin transfection reagent and FreeStyle Media (Thermo Fisher Scientific, Waltham, MA). Supernatants were purified using Streptactin XT Resin purification (IBA Lifesciences ...
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bioRxiv - Cell Biology 2020Quote: ... sheep anti-rabbit-HRP (Invitrogen), or goat anti-rabbit or goat anti-mouse IRdye 680 (1:20.000 ...
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bioRxiv - Microbiology 2019Quote: ... sheep polyclonal rotavirus antiserum (Invitrogen), Alexa Fluor 546-conjugated anti-mouse IgG (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... sheep anti-rabbit-HRP (Invitrogen), or goat anti-rabbit or goat anti-mouse IRdye 680 (1:20.000 ...
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bioRxiv - Microbiology 2020Quote: ... 5% sheep’s blood (ThermoFisher, USA), pH 7) ...
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bioRxiv - Microbiology 2022Quote: ... anti-sheep-HRP (A16041, Invitrogen). Antibodies used for immunofluorescence staining are ...
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bioRxiv - Developmental Biology 2023Quote: ... siPROX1 for sheep (Invitrogen; 10620310), or siSOX9 for porcine (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-sheep 488 (Invitrogen, #A11015).
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bioRxiv - Molecular Biology 2021Quote: ... (2020; Figure S1; Life Technologies). The construct was cloned into pGEM®-T Easy (Promega Corp. ...
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bioRxiv - Microbiology 2021Quote: ... S1 Nuclease (Thermo Fisher Scientific) digestion was subsequently performed to de-branch chimeric DNA formed during the rolling circle amplification process ([5]) ...
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bioRxiv - Microbiology 2020Quote: ... S1 nuclease (ThermoFisher Scientific, EN0321) treatment was carried out after inactivation of protease with 0.56 u/µl nuclease in S1 nuclease buffer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 10 units S1 Nuclease (Invitrogen) in S1 Buffer (30 mM sodium acetate pH 4.6 ...
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bioRxiv - Cell Biology 2023Quote: ... S1 nuclease (Invitrogen cat # 18001016) was diluted 1/100 in S1 buffer and used at 20 U/ml ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 2% heat-inactivated FCS and 15 mM HEPES (Gibco). Intestines were cut open longitudinally ...
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bioRxiv - Immunology 2023Quote: ... and 2% v/v Fc receptor binding inhibitor (Thermo Fisher Scientific)) ...
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bioRxiv - Cancer Biology 2023Quote: ... containing either 10 or 20% FCS (Bodinco) and 2% PSF (Gibco) and were passaged twice a week ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 100 μL of FC buffer containing primary antibodies (mouse anti-Spike S2, Thermo Fisher, Cat #: MA5-35946, 1:500 dilution) (mouse anti-Flag M2 ...
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bioRxiv - Cancer Biology 2021Quote: ... The secondary antibodies used for detection were horseradish peroxidase (HRP)–conjugated goat anti-mouse and goat anti-rabbit IgG (1:5,000; Thermo Scientific). The blots were incubated with Luminata Western HRP substrate (Millipore ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... The primary antibody was detected by an appropriate secondary antibody conjugated with horseradish peroxidase (Thermo Fisher Scientific, Waltham, MA, USA) and visualized by ECL kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Secondary antibody of Goat anti-rabbit conjugated to horseradish peroxidase at 1:1500 dilution was used (Cat. #32460 Thermo Scientific Pierce Goat anti-Rabbit IgG ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were subsequently rinsed and incubated for 1 h at RT with the appropriate HorseRadish-Peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The visualization of the signal was conducted using Immobilon® Forte Western HRP Substrate (Millipore ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was then incubated with a goat-anti-rabbit secondary antibody conjugated to horseradish peroxidase (Life Technologies, 1:5000) or a goat-anti-mouse secondary antibody conjugated to horseradish peroxidase (Cell Signaling ...
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bioRxiv - Microbiology 2021Quote: ... The immunoblots were analyzed using primary antibodies incubated overnight at 4°C and secondary antibodies linked to horseradish peroxidase (HRP) (Invitrogen), and after each step immunoblots were washed 4x with TBST ...