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Citations for Thermo Fisher :
9951 - 10000 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... OPP-Puro samples were processed for Click-chemistry detection with Alexa Fluor 647 picolyl azide as per the instructions in the Click-iT® Plus OPP Protein Synthesis Assay Kit (Invitrogen cat. no. C10458). Samples were then analyzed by flow cytometry ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The subset of individuals used in the 3RAD library prep (see below) were also quantified using the fluorescence-based Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen - Molecular Probes, Eugene, OR, USA). Extracted DNA were stored at −20°C until use.
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bioRxiv - Evolutionary Biology 2020Quote: Purified extracted DNA of modern samples was quantified with a Qubit 2.0 Fluorometer and dsDNA HS Assay kit (Life Technologies, Grand Island, NY, USA) and ca ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We quantified total RNA concentration (ng/μL) using a Qubit RNA HS assay kit on a Qubit 4 Fluorometer (Thermofisher Scientific, Waltham, MA, USA). We used a 4200 TapeStation Analyzer System and High Sensitivity RNA ScreenTape assays (Agilent ...
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bioRxiv - Genomics 2020Quote: ... RNA quality and concentration was then analysed on a Qubit® 2.0 Fluorometer using Qubit RNA BR assay kit (Invitrogen, Life Technologies, Carlsbad, CA, USA). Between 49 and 107 million sequence reads were generated for each sample on an Illumina HiSeq 4000 platform with a single-end sequencing primer over 150 cycles (Additional file 5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were spun down with a tabletop centrifuge and the protein concentration was determined using the BCA protein assay kit (Thermo Fisher Scientific, Cat: A532225) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA-depleted and purified RNA was used to construct double-stranded DNA library using MGIEasy RNA Library preparation reagent set (MGI ...
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bioRxiv - Genomics 2020Quote: ... and 4 μL was used for concentration measurement using the Qubit Fluorometer and Qubit dsDNA HS reagents Assay kit (Thermo Fisher Scientific, Waltham, MA). We finally combined the three extracts to reach DNA quantities sufficient for library preparation ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were centrifuged at 12,000 × g for 10 min at 4°C and the supernatant was isolated and quantified using a Pierce BCA protein assay kit (Thermo Fisher Scientific, MA, US). Loading buffer (4× ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was examined by 5-bromo-2-deoxyuridine (BrdU) cell proliferation enzyme-linked immunosorbent assay (ELISA) according to the kit manufacturer’s protocol (Life Technologies, Grand Island, NY, USA). The cells were incubated in presence of various concentrations of CLEFMA (0 – 10 µM) ...
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bioRxiv - Biochemistry 2020Quote: ... assay as determination of protein concentration by measurement of absorbance at 280 nm relies on the presence of tryptophan (Pierce™ BCA Protein Assay Kit-Reducing Agent Compatible; ThermoFisher Scientific; Waltham, MA).
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bioRxiv - Plant Biology 2021Quote: ... The final concentration of DNA samples was measured using Qubit 2.0 Fluorometer dsDNA BR Assay kits (Life Technologies-Thermo Fisher Scientific, Saint Aubin, France) and the final fragment size distribution using an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... Concentration and quality were analyzed using a NanoDrop™ 1000 spectrophotometer and a Qubit 4 Fluorometer with a dsDNA HS Assay Kit (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Systems Biology 2021Quote: The H2O2 level in the extracellular medium was quantified with Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit (Invitrogen, Carlsbad, USA, Catalog no. A22188). 500 μL of cell suspension were spinned down after overnight growth in LB medium ...
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bioRxiv - Neuroscience 2020Quote: ... Lysate was centrifuged at 14,000g for 5min at 4°C and protein concentration was measured using Pierce BCA protein assay kit (Thermo Fisher Scientific, MA, USA PI23227) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The library fragment sizes were measured using agarose gel electrophoresis and the concentrations using Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, California, USA) and NEBNext Library Quant Kit for Illumina (New England BioLabs ...
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bioRxiv - Bioengineering 2020Quote: ... N=3 trials with different ASC donors) for analysis of total cell density using the Quant-iT® PicoGreen® dsDNA Assay Kit (ThermoFisher, Waltham, USA). For all trials ...
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bioRxiv - Microbiology 2021Quote: ... Concentrations of the libraries were determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Darmstadt, Germany). Sequencing was performed by using the HiSeq2500 instrument (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: ... The quantitative real-time PCR assay was performed with SuperScript III Platinum SYBR Green One-Step qRT-PCR kit (Thermo Scientific, Wilmington, DE, USA). For each sample ...
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bioRxiv - Cell Biology 2021Quote: ... Each sample was measured for protein content using the PierceTM BCA protein assay kit-reducing agent compatible per manufacturer’s instructions (23250, Thermo Fisher Scientific, Waltham, MA, US). 100 ug of protein was used per sample ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantification of RNA quantification was carried out using Qubit® RNA Assay Kit and Qubit® 2.0 Fluorometer (Life Technologies, San Diego, CA, USA).
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bioRxiv - Microbiology 2022Quote: WGS performed at CFIA used extracted nucleic acid quantified using the Qubit™ RNA High Sensitivity (HS) Assay Kit on a Qubit™ Flex Fluorometer (Thermo Fisher Scientific). 11uL or 200ng of total RNA was subject to DNase treatment using the ezDNase™ Enzyme (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The enriched libraries were then quantified using the Qubit™ 1x dsDNA HS Assay Kit on a Qubit™ Flex Fluorometer (Thermo Fisher Scientific) and subsequently pooled in equimolar amounts prior to fragment analysis on 4200 TapeStation System using the D5000 ScreenTape Assay (Agilent) ...
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bioRxiv - Physiology 2022Quote: The volume of tears per minute (µl / min) and the protein concentration in tears (mg / ml) using the BCA protein assay kit (Fisher Scientific, reference 10678484, France) were measured and expressed as the mean ± SD.
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bioRxiv - Evolutionary Biology 2022Quote: ... The samples of individual gels were pooled into approximately equimolar subpools as indicated by band intensity and measured with the Qubit dsDNA br Assay Kit (Life Technologies GmbH, Darmstadt, Germany). Subpools were mixed in an equimolar fashion and stored at -20 °C until sequencing ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Germany) and exact concentration was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Darmstadt, Germany). Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Concentration of the libraries were determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Darmstadt, Germany). Sequencing was performed on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... microbial protein fermentation was assessed by measuring the protein concentration using Thermo Scientific™ Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA) and the ammonia concentration using Sigma-Aldrich® Ammonia Assay Kit (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration in the resulting tissue lysate supernatants was measured using the Pierce™ BCA assay kit (Cat. No. 23227, Thermo Scientific, Rockford, IL), and samples were aliquoted and stored at −80°C until analysis.
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bioRxiv - Bioengineering 2021Quote: ... microbial protein fermentation was assessed by measuring the protein concentration using Thermo Scientific™ Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA) and the ammonia concentration using Sigma-Aldrich® Ammonia Assay Kit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... DNA extracts were quantified with Qubit™dsDNA BR Assay Kit and a Qubit 4.0 fluorometer (Invitrogen, Thermo Fisher Scientific, Waltham, MA, United States). Novogene Europe Co. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The concentrations of the pooled RNA samples were measured using the Qubit™ RNA HS Assay Kit and a Qubit™ 4 fluorometer (ThermoFisher, Schwerte, Germany). Samples from Tenebrio molitor and Calliphora vicina were qualified with the High Sensitivity RNA ScreenTape Assay Kit and the Agilent 4200 TapeStation system (Agilent ...
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bioRxiv - Genomics 2022Quote: ... DNA quality was checked by electrophoresis on 1% agarose gels and DNA concentration was measured using a Qubit® dsDNA HS Assay Kit in Qubit® 2.0 Fluorometer (Life Technologies, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... DNA was quantified and quality checked with the Qubit fluorometer or the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Massive parallel sequencing was performed using targeted-capturing of the 165 genes included in the “Solid and Haematological tumors” panel (BRIGHTCore ...
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bioRxiv - Cell Biology 2022Quote: ... cells that had incorporated EdU were fluorescently labeled using the Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Thermo Fisher # C10632) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Labeling and detection of apoptotic cells was completed using the Invitrogen Click-iT Plus TUNEL Assay for In Situ Apoptosis Detection 488 kit (Invitrogen, Waltham, MA, catalog #: C10617) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... or specific Taqman microRNA assay primers with a TaqMan® MicroRNA Reverse Transcription Kit for the assessment of microRNAs (4366596, Applied Biosystems™, Thermo Fisher). Downstream qPCR amplifications of first-strand cDNA were performed using TaqMan® Universal PCR Master Mix ...
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bioRxiv - Microbiology 2020Quote: ... The purified sample was recovered spinning for 2 minutes at 1000 x g and finally quantified using the Pierce™ BCA Protein Assay kit (ThermoFisher Scientific, Monza, Italy) and stored in aliquots at −80°C until use ...
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bioRxiv - Cell Biology 2020Quote: ... DNAse treatment using Invitrogen TURBO DNA-free rigorous procedure was performed before measuring RNA concentration with a Qubit® RNA HS Assay Kit (Molecular Probes, Life Technologies). Reverse transcription reactions used 1.32 μg of RNA using SuperScript III Reverse Transcriptase (Invitrogen ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... followed by chloroform-methanol protein precipitation to obtain pure protein pellets that were subsequently resuspended in digestion buffer (100 mM TEAB with 1% (v/v) SDC) and quantified by the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, United States). From each sample ...
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bioRxiv - Microbiology 2021Quote: ... Lentivirus infectivity was determined 48 hours post-infection by measuring Gaussia luciferase activity in the 293T-ACE2 supernatant using a Pierce™ Gaussia luciferase flash assay kits (ThermoFisher Scientific, cat# 16158).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pelleted and stained with the Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Thermo Fisher Scientific, C10632), according to the manufacturer protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of the bacteriocin was measured after each step of protein purification using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The concentration of the mitochondrial DNA standard was measured by Qubit 1XdsDNA HS Assay Kit with Qubit 4 Fluorometer (Thermo Fisher Scientific, Waltham, MA). For the experiment described in Supplemental Figure 4 ...
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bioRxiv - Plant Biology 2021Quote: ... Concentrations of proteins in plant leaves were determined according to the Pierce BCA Protein Assay Kit-Reducing Agent Compatible (Thermo Scientific, #23250, Rockford, IL).
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bioRxiv - Cell Biology 2021Quote: Cell cycle progression of NALM6 cells was assessed using Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Invitrogen cat # C10425), following manufacturer instructions ...
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bioRxiv - Genetics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). After human DNA-depletion ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The samples of individual gels were pooled into approximately equimolar subpools as indicated by band intensity and measured with the Qubit dsDNA br Assay Kit (Life Technologies GmbH, Darmstadt, Germany). Subpools were mixed in an equimolar fashion and stored at −20 °C until sequencing ...
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bioRxiv - Genetics 2021Quote: ... The concentration of total RNA was measured using the Qubit RNA Assay Kit in a Qubit 2.0 Flurometer (Life Technologies, Carlsbad, CA, United States). RNA samples that met the criteria of having an RNA integrity number (RIN ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated in lysis buffer for 30 min at 4°C with continuous shaking and then centrifuged to collect clear supernatant for protein quantification was done using Pierce™ BCA protein assay kit (Thermo Fisher Scientific Inc.). Primary antibodies used ...