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Citations for Thermo Fisher :
9701 - 9750 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Total nucleic acid concentrations and purity were measured using a Qubit 4 fluorometer with RNA/dsDNA Broad Range (BR) assay kits (Cat. No. Q10210, Q32850, Life Technologies, Carlsbad, CA) and a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was measured in the supernatant using a Pierce bicinchoninic acid protein assay kit (Cat. No. 23225, ThermoFisher Scientific, Burnaby, BC, CA). Lysates were incubated in Laemmli loading buffer (Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% SDS and 12% glycerinum) and protein concentration was quantified using the Pierce BCA Protein Assay kit (Thermo Scientific, Catalogue#23227, Waltham, MA) 20 µg proteins were electrophoresed in running buffer on Mini-protean® TGXTM Precast Gel (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... The eluate containing the purified cDNA construct was first quantified using the 1X dsDNA HS Assay Kit (Q33231, Thermo Fisher Scientific, CA, USA) on a Qubit 4.0 Fluorometer ...
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bioRxiv - Bioengineering 2024Quote: ... Correct formation of disulfide bonds in this environment was assessed with synthesis of Gaussia luciferase and luminescence measurement with a Pierce Gaussia Luciferase Glow Assay Kit (16160, Thermo Fisher Scientific, USA). The sequence of RBD2 used throughout this work contains seven Cysteine ...
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bioRxiv - Cell Biology 2024Quote: ... Detection of EdU incorporation into the DNA was performed with the Click-iT® EdU Alexa Fluor® 647 Cell Proliferation Assay Kit (Invitrogen, C10340) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was eluted in 20 µL RNase-free water and total RNA was quantified with the Qubit RNA HS Assay Kit (Thermo Scientific, Cat. #Q32855). RNA quality was measured using the Bioanalyzer RNA 6000 Assay (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: Cells were stained with FITC-annexin V and propidium iodide using the Vybrant Apoptosis Assay Kit #3 (V13242; Molecular Probes/Invitrogen, Carlsbad, CA). For a positive control ...
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bioRxiv - Cancer Biology 2024Quote: Cells were stained with FITC-annexin V and propidium iodide using the Vybrant Apoptosis Assay Kit #3 (V13242; Molecular Probes/Invitrogen, Carlsbad, CA). For a positive control ...
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bioRxiv - Cell Biology 2024Quote: Protein concentration of myotube lysates or EV-treated myoblast whole cell lysates was determined using Pierce™ BCA protein assay kit (Thermo Fisher Scientific) following the manufacturer’s instructions as described before [41] ...
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bioRxiv - Developmental Biology 2024Quote: Isolated proteins from nuclear extracts either from cell pellet or whole tissue were quantified using PierceTM Protein Assay Kit (Thermo Scientific, Rockford, IL). Each sample protein was supplemented with 4× lithium dodecyl sulfate (LDS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The protein concentration was determined by the BCA (bicinchoninic acid assay) assay (Thermo Fisher Scientific, Waltham, MA, USA) and homogenized at 0.1 mg ml −1 ...
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bioRxiv - Bioengineering 2020Quote: ... Cytokine concentration was normalized to total protein content quantified using a bicinchoninic acid assay (BCA assay, Thermo Fisher). The final sample sizes for each group were ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentration in each sample was determined using a BCA assay (Pierce BCA Protein Assay, Thermo Fisher Scientific). Protein lysates (10 μg ...
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bioRxiv - Bioengineering 2022Quote: ... Subsequent TUNEL assay for apoptosis was performed using Click-iT TUNEL Alexa Fluor 488 Imaging Assay (Thermo Fisher) following manufacturer’s protocol ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... and measured using a bicinchonic acid (BCA) protein assay (Pierce BCA Protein Assay; Thermo-Fisher Scientific, Toronto, Canada). All protein extracts were re-suspended in sample buffer and size-fractioned by SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... probes and VIC-labeled Rpl13a probe as a reference (Taqman Gene Expression Assay, ThermoFisher, Assay ID: Mm01612986-gH) and the TaqMan Universal Master Mix II ...
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bioRxiv - Bioengineering 2022Quote: ... Cel-miR-39-3p expression was quantified using a TaqMan Gene Expression assay (ThermoFisher Scientific, Assay Id: Hs01125301_m1) on a Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... from one litter by TaqMan qPCR (Cdk6 probe assay ID# Mm01311342_m1 and housekeeping Gapdh probe assay ID# Mm99999915_g1, ThermoFisher). 66.9% reduction in Cdk6 expression was measured in Cdk6iECKO (n=4 ...
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bioRxiv - Microbiology 2023Quote: ... and to confirm the validity of the MTS assay were repeated using an alamarBlue assay (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... A Custom TaqMan Gene Expression RT-PCR assay designed for the gag-pol region (Assay ID: AP7DXHY; ThermoFisher) was then used on the cDNA to quantify the HIV-1 viral copy number ...
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bioRxiv - Cell Biology 2024Quote: ... Sample protein content was determined with the Pierce detection assay (Pierce 660 nm Protein Assay Reagent (ThermoFisher Scientific), complemented with Ionic Detergent Compatibility Reagent (IDCR ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 µl of TaqMan Assay Netrin 1 (Assay-ID: Netrin 1 Hs00924151_m1 FAM-MGB, Applied Biosystems; Darmstadt, Germany) and 0.5 µL nuclease free water in a total volume of 6.5 µl ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration of each sample was determined using the BCA protein assay (Pierce™ BCA Protein Assay, Thermofisher). 40 micrograms of protein per lane were prepared in SDS sample buffer (50 mM Tris pH 6.8 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved by SDS-PAGE and transferred onto a PVDF membrane (pore size 0,2 μm, Merck, cat. #GE10600021. The membrane was blocked in 5% BSA (Thermo Fisher Scientific, cat. #11423164)/TBS-T (0,1% Tween in TBS ...
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bioRxiv - Bioengineering 2023Quote: ... and transferred onto an iBlot™2 nitrocellulose transfer mini stack membrane (for Cas9) or iBlot™2 PVDF transfer mini stack membrane (for AcrIIA4) using the iBlot2 dry transfer instrument (Thermo fisher scientific). For Cas9 transfer ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were transferred to 0.45 micron nitrocellulose membranes (Protran NBA085C001EA) via wet membrane transfer at 100V for 1 hr 15 minutes in NuPage transfer buffer (Life Technologies; NP0006-1) supplemented with 10% methanol and 0.1% SDS ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were transferred to 0.45 micron nitrocellulose membranes (Protran NBA085C001EA) via wet membrane transfer at 100V for 1 hr 15 minutes in NuPage transfer buffer (Life Technologies; NP0006-1) supplemented with 10% methanol and 0.1% SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... quantified by Qubit assay (Thermo Fisher Scientific) and pooled ...
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bioRxiv - Cancer Biology 2021Quote: ... using Taqman Gene Expression Assays (Applied Biosystems). Gene expression data were normalized to 18S rRNA.
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bioRxiv - Molecular Biology 2020Quote: ... and TaqMan MicroRNA Assays (Thermo Fisher Scientific). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2020Quote: ... or TaqMan Gene Expression Assays (Applied Biosystems). All procedures were performed according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... using TaqMan Gene Expression Assay (Applied Biosystems) with FFAR3 hydrolysis probe (Mm02621638_1 ...
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bioRxiv - Cell Biology 2020Quote: ... or Taqman Gene Expression Assay (Applied Biosystems) was used as the primer ...
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bioRxiv - Cell Biology 2020Quote: ... TaqMan® MicroRNA Assays (Applied Biosystems, USA) was used to test miR-1251 expression ...
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bioRxiv - Developmental Biology 2020Quote: ... After quantification by BCA assay (ThermoFisher Scientific), 150µg of total protein was loaded per well for each genotype ...
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bioRxiv - Evolutionary Biology 2021Quote: ... quantified it (Qubit HS dsDNA assay, Invitrogen), and prepared paired-end libraries from each line using the Illumina Nextera XT DNA library preparation kit per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... A micro-BCA protein assay (Thermo Fisher) was performed in plates coated with Ag and washed to demonstrate that all peptides bound to the well surfaces in the plates (data not shown) ...
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bioRxiv - Molecular Biology 2021Quote: ... and analyzed using TaqMan Assays (Applied Biosystems) with a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-Developed TaqManTM Assay Reagents (Applied Biosystems) were used for human ACTB as an internal control ...
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bioRxiv - Molecular Biology 2021Quote: ... and TaqMan gene expression assays (Applied Biosystems) on a Quant studio 3 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... with TaqMan gene expression assays (Applied Biosystems) using standard techniques ...
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bioRxiv - Bioengineering 2020Quote: ... or the resazurin-based PrestoBlue assay (ThermoFisher). For both assays ...
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bioRxiv - Physiology 2020Quote: ... or radioimmunoprecipitation assay buffer (RIPA, 89901, ThermoFisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... and 1X TaqMan primer assay (ThermoFisher Scientific). Cycling conditions were 2 min at 50°C ...
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bioRxiv - Microbiology 2021Quote: ... or the Fast SYBR Green Assay (ThermoFisher) on an ABI Fast 7500 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... quantified using PicoGreen dsDNA Assay (Thermo Fisher). All 14 x amplicon products from a given sample were then pooled at equal abundance and partitioned into separate aliquots for analysis by shortread and nanopore sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... protein estimated with BCA assay (Thermo Fisher) and 10 μg total protein separated on 4-15% TGX stain-free protein gels (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... using TaqMan Gene Expression Assays (Thermo Fisher) for Ppargc1a (Pgc-1α ...
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bioRxiv - Neuroscience 2020Quote: ... using TaqMan Gene Expression Assays (Thermo Fisher) for Cox2I (Mm03294838_g1 ...