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Citations for Thermo Fisher :
9451 - 9500 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were exposed to enhanced chemiluminescence reagents (Thermo Scientific) and imaged using the BioRad Chemidoc (Hercules ...
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bioRxiv - Developmental Biology 2024Quote: ... and transferred onto a PVDF membrane (Life Technologies, LC2002). The membrane was then sectioned based on the molecular weights of the markers being analyzed ...
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bioRxiv - Developmental Biology 2021Quote: ... The final library size distribution was validated with capillary electrophoresis and quantified with a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, P11496) and qPCR ...
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bioRxiv - Cell Biology 2020Quote: Cell proliferation in culture was measured by the CyQUANT® cell proliferation assay kit as per the protocol described by the provider (Thermo Fisher scientific). In brief ...
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bioRxiv - Cell Biology 2020Quote: SA-β-gal levels in the chondrocytes after senescence induction and monolayer culture were evaluated using the CellEvent™ Senescence Green Flow Cytometry Assay Kit (C10840; Thermo Fisher Scientific). The working solution was prepared by diluting the CellEvent™ Senescence Green Probe (1,000X ...
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bioRxiv - Immunology 2021Quote: Bone marrow B factions were sorted and calcium influx was assayed with a Fluo-4 Direct™ Calcium Assay Kit (ThermoFisher cat# F10471). Briefly cells were incubated with Fluo-4 Direct™ at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: Cell cycle analysis was performed using the Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol (Beaudin et al. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA samples were quantified with a Qubit® 2.0 Fluorometer using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Libraries were prepared using the TruSeq® Nano DNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... The extracted nucleic acids were quantified with a Qubit Fluorimeter and the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and stored at −80°C.
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bioRxiv - Bioengineering 2022Quote: ... Lysates were clarified by centrifugation (20,000 × g, 15 min) and protein concentration was determined (BCA protein assay Kit, Pierce®, Thermo scientific, IL, USA). The same protein amount (usually 20-30 μg ...
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bioRxiv - Microbiology 2022Quote: ... followed by quantification of double stranded DNA product using Quantit dsDNA Assay Kit Broad range and Nanodrop (Thermo Fisher Scientific, Waltham, MA, USA). Host DNA was sequenced using Illumina MiSeq at the Earlham Institute ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was eluted in 50 µL of buffer EB and quantified with a Qubit HS dsDNA Assay Kit (Life Technologies, Carlsbad, CA, USA), with dsDNA concentrations ranging from 4.1 to 11.8 ng/μL.
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were analysed on Agilent 2200 TapeStation System using high sensitivity D1000 screentapes and quantified using the Qubit Fluorometer High Sensitivity dsDNA assay kit (Thermo Fisher Scientific, Q32851). Multiplexed libraries were sequenced on a NextSeq-500 Illumina platform using the NextSeq 500/550 High Output v2 kit 75 cycles (FC-404–2005).
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bioRxiv - Molecular Biology 2020Quote: ... RNA libraries were analysed on Agilent 2200 TapeStation System using high sensitivity D1000 screentapes and quantified using the Qubit Fluorometer High Sensitivity dsDNA assay kit (Thermo Fisher Scientific, Q32851). Multiplexed libraries were sequenced on a NextSeq-500 Illumina platform using the NextSeq 500/550 High Output v2 kit 75 cycles (FC-404– 2005).
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bioRxiv - Molecular Biology 2021Quote: ... Obtained amplicon libraries (around 420 bp long) were then quantified by the Quant-iTTM PicoGreen® dsDNA Assay Kit (Invitrogen, Carlsbad, CA, USA) using the NanoDrop™ 3300 Fluorospectrometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and DNA concentration was measured using a Qubit® 2.0 Fluorometer (following the Invitrogen™ Qubit™ dsDNA BR Assay Kit) (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... 10 min) and cell-free supernatants were snap-frozen for total protein analysis using the Pierce™ BCA protein assay kit (Thermo Scientific; #23227). The pellet was resuspended with 400 μL of PBS and total leukocyte count was measured via a hemocytometer (Neubauer chamber) ...
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bioRxiv - Genomics 2020Quote: ... The DNA was stored at 4°C overnight to facilitate DNA homogenization and then quantified using a Qubit dsDNA HS Assay Kit (Molecular Probes/Life Technologies). The labeled DNA was stained with an intercalating dye and left to stand at room temperature for at least 2 hrs before loading onto a Bionano Chip.
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bioRxiv - Genomics 2020Quote: ... SDS) for 10 minutes at 95°C and protein concentration was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific 23227). Protein samples were separated by SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ gels (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific mRNA synthesis and degradation kinetics were measured by a pulse-chase assay using the Click-iT Nascent RNA Capture Kit (Thermo Fisher Scientific, #C10365) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... SEAP concentration was tested on 50µl media supernatants of the samples using CPSD (Tropix PhosphaLite Chemiluminescent Assay Kit, Life Technologies cat no. T1017) using a minor variant of the manufacturer’s instructions and luminescence intensity was measured using a Varioskan Flash luminometer (Thermo Fisher) ...
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bioRxiv - Genomics 2021Quote: ... Extracted DNA samples were assessed for quality using the Agilent Tape Station and quantified using the Qubit™ dsDNA BR Assay kit (cat. #Q32850) with the Qubit 2.0® fluorometer (Life Technologies™). Purified DNA was subjected to both long-read (Nanopore GridION-X5 sequencer ...
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bioRxiv - Cell Biology 2021Quote: ... The Click reaction was performed using the Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit according to manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... pre-stained cells were loaded with Fluo-4 via by mixing with equal volumes of 2X Fluo-4 Direct™ loading solution (Fluo-4 Direct™ Calcium Assay Kit, Thermo Fisher). After a 30 min incubation at 37°C and then 30 mins at RT ...
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bioRxiv - Microbiology 2021Quote: ... The nucleic acid concentration and purity were assessed using Qubit dsDNA high sensitivity and RNA assay kits in a Qubit 4 fluorometer (Invitrogen, Carlsbad, CA, USA), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... 5 ×105 cells with or without transduction and their supernatants were harvested with RIPA buffer and the protein concentration was determined with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). Equal amounts of protein (50 µg ...
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bioRxiv - Bioengineering 2020Quote: The viability of cells was quantified using the Live/Dead® viability assay kit (Molecular Probes®, Thermo Fisher Scientific, Whaltam, MA, USA). After one PBS wash ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated with 100 μl of fresh medium plus 10 μl of the 12 mM MTT stock solution from the Vybrant® MTT cell proliferation assay kit (#V13154, Thermo Fisher Scientific) in the presence of the control vehicle or 30 μM cisplatin ...
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bioRxiv - Bioengineering 2021Quote: ... the tissues were lyophilized to estimate the ratios of ng DNA per mg dry tissue in each group using Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Carlsbad, CA, USA) and a SpectraMax M Series Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Bioengineering 2021Quote: ... The total protein concentration of the EVs was measured using the Pierce Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA) to estimate the concentration and the yield ...
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bioRxiv - Systems Biology 2020Quote: ... The calculated mRNA abundance was then scaled to the total RNA content measured by Qubit RNA HS Assay Kit (Thermo Fisher, Waltham, MA). For proteomics ...
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bioRxiv - Evolutionary Biology 2021Quote: Four randomly selected samples from each of three bat species underwent additional quantification using an Invitrogen Qubit 3.0 Fluorometer and the Qubit RNA HS Assay Kit (ThermoFisher Scientific, Carlsbad, CA, USA). After quantification ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The soluble fraction was separated by centrifugation at 14,800 x g for 5 min and the protein concentration determined using the Pierce BCA assay kit (Thermo Scientific™ Pierce™). The protein concentration of each sample was adjusted to 693 µg/mL ...
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bioRxiv - Microbiology 2021Quote: The neuraminidase (NA) activity of NDV LaSota and rNDV-H5 was determined using the NA-fluor Influenza Neuraminidase Assay kit (Applied Biosystems, CA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2021Quote: ... All isolated DNA was assessed for concentration and quality by fluorometry and electrophoresis using a Qubit™ 4 and Qubit™ 1X dsDNA HS Assay Kit (ThermoFisher Scientific) and a TapeStation (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatants were transferred into new tubes and protein concentrations were quantified using the Pierce BCA Assay Protein kit (Thermo Fisher Scientific – 23225). 60μg total protein were mixed with SDS gel loading buffer ...
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bioRxiv - Systems Biology 2021Quote: ... marinus cells was estimated from exponential bioreactor cultures using the fluorometric Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher, Waltham, Massachusetts, US). A sample of freeze-dried biomass (approx ...
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bioRxiv - Microbiology 2020Quote: ... were smeared with fixed blood and stained overnight in the reaction mix from the Apo BrdU TUNEL Assay Kit (Molecular Probes, Eugene, OR). TUNEL-labeled DNA was detected using a biotinylated anti-BrdU antibody and streptavidin-Alexa Fluor 594 conjugate (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The results were normalized using total protein content of the cells that was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Experiments have been performed in triplicates in five independent experiments.
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bioRxiv - Cancer Biology 2021Quote: Nitrite and hydrogen peroxide concentrations were quantified using Griess reagent for nitrite and Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit (ThermoFisher, Oregon, USA), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time PCR was performed using TaqMan gene expression assays after cDNA preparation using the high capacity reverse transcription kit (Applied Biosystems; cat#4368814). β-actin and tata-box binding protein (TBP ...
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bioRxiv - Cancer Biology 2020Quote: The concentration of H2O2 in PAI was measured using the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... The irradiation effect on tendon cells was detected with a live/dead cell viability assay kit (Cat # L-7013, Life Technologies, Carlsbad, CA) performed according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... the total protein concentration was quantified and normalized in all samples using the Pierce BCA protein assay kit (ThermoFisher Scientific, Waltham, MA, USA). Results were expressed as median fluorescence intensity (MFI).
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bioRxiv - Neuroscience 2022Quote: ... v/v) before LC-MS/MS analysis according to the total protein content (quantified using BCA protein Assay Kit (Thermo Scientific, Massachusetts, USA).
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration for all samples (culture media, PBS rinses, cytosol, and pellet) was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford IL) and 20 µg of protein from each sample was run on an SDS PAGE gel and transferred to a PVDF membrane ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the supernatant was collected and the cleared lysate protein concentration was quantified using the BCA protein assay kit (Thermo Scientific, Pierce Rockford, IL) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Total protein concentration in lysates (supernatants) was determined using the BCA method (Pierce™ BSA Protein Assay Kit, Thermo Fisher Scientific, Waltham, MA). Proteins (20-30 μg ...
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bioRxiv - Pathology 2022Quote: ... and non-esterified fatty acids (NEFA) were assessed by commercial assay kits (Jiancheng, Nanjing, China) and monitored with a NanoDrop 2000 Spectrophotometer (Thermo Scientific, Wilmington, DE).
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bioRxiv - Molecular Biology 2022Quote: ... 5ul of the DNA was used to measure the DNA concentration using Qubit fluorometer with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific Q32854).