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Citations for Thermo Fisher :
9651 - 9700 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... pH 8) was added on each pellet and total protein quantification was performed using BCA Protein Assay kit (Pierce™, Thermo Fisher Scientific). Before immunobloting analysis ...
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bioRxiv - Neuroscience 2023Quote: The amount of LDH in the cell lysates and media was measured using CyQUANT LDH Cytotoxicity Assay kit (Thermo Fisher Scientific, Waltham, MA). The percentage of cell death was calculated by the total amount of LDH in the media divided by that in the cell lysates.
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bioRxiv - Biochemistry 2023Quote: ... The concentrations of recombinant proteins were determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Cat#A53225, Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Bioengineering 2023Quote: ... by washing in phosphate buffered saline (PBS) and storing films at −80 °C for DNA quantification using Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher Scientific), per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were cleared by centrifugation at 18,000 x g and 4°C for 20 min and protein concentration was determined using the BCA Protein Assay Kit from Pierce™ (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... EVs from non-treated iRBCs and EVs from CQ-treated iRBCs were added containing 300 μg of protein which was calculated using a BCA assay kit (Thermo Fisher Scientific, USA). 1x PBS was used as the control without EVs ...
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bioRxiv - Genetics 2023Quote: ... and total protein concentration was estimated using Pierce Bicinchoninic Acid (BCA) protein assay kit according to the manufacturer’s protocol (cat. 23225, Thermo Fisher Scientific, IL, USA).
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bioRxiv - Evolutionary Biology 2023Quote: ... each IgG was quantified using the Pierce™ BCA Protein Assay Kit as per the manufacturer’s recommendations (Thermo Fisher Scientific, cat. no. 23225).
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bioRxiv - Physiology 2023Quote: ... the tissues were lyophilized to estimate the ratios of ng DNA per mg dry tissue in each group using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Carlsbad, CA, USA) and a SpectraMax M Series Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Evolutionary Biology 2023Quote: ATP levels were quantified in cell lysates using a luciferin/luciferase bioluminescence assay (ATP determination kit, Molecular Probes cat # A22066, Thermo Fisher Scientific) as previously described (Jara et al ...
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bioRxiv - Evolutionary Biology 2023Quote: ATP levels were quantified in cell lysates using a luciferin/luciferase bioluminescence assay (ATP determination kit, Molecular Probes cat # A22066, Thermo Fisher Scientific) as previously described (Jara et al ...
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bioRxiv - Biochemistry 2023Quote: α-synuclein was quantified in the striatum region of mouse brain utilizing the Enzyme-linked Immunosorbent Assay (ELISA) Kit from Invitrogen (Cat No. KHB0061) following the manufacturer‘s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... we quantified the cDNA concentration of a subset of samples using th eQuant-iT Picogreen dsDNA Assay kit (Thermo Fisher, Waltham, MA, USA). Standards ranging from 25pg/mL to 25ng/mL were prepared using the Lambda Standard DNA (100ug/mL ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA quality and concentration were estimated with NanoDrop One C spectrophotometer and Qubit 4MT with a dsDNA HS Assay Kit (Invitrogen, Carlsbad, CA, USA). Total of 83 Lonsdalea quercina strains (one strain per tree ...
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bioRxiv - Bioengineering 2023Quote: ... the supernatants were transferred to clean tubes and their protein concentrations were measured using a BCA Protein Assay Kit (Pierce Thermo Scientific, MA, USA), according to the manufacturer’s instructions ...
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Impaired yolk sac NAD metabolism disrupts murine embryogenesis with relevance to human birth defectsbioRxiv - Developmental Biology 2024Quote: ... Insoluble proteins and other debris were removed by centrifugation at 12000 rpm for 7 minutes in a microcentrifuge and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, USA) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... After incubation cells were harvested and fixed and the labelling of EdU incorporation was conducted by Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Molecular Probes). EdU-Alexa Fluo 488 positive cells were detected by flow cytometry on Quanteon analyzer and data analysis was performed using FlowJo_V10.8.1.
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bioRxiv - Genomics 2024Quote: ... DNA quantity and quality were assessed using the Nanodrop 2000 spectrometer and Qubit 4 fluorometer with the dsDNA BR assay kit (Invitrogen, Carlsbad, CA, USA), and integrity was confirmed by gel electrophoresis ...
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bioRxiv - Bioengineering 2024Quote: To measure the mOx40L protein abundance in EVs the Mouse TNFSF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) kit (Invitrogen, Thermo Fisher Scientific) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Purity and concentration of the recombinant proteins was assessed by SDS-PAGE and the Qubit Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Gel filtration was used to recover the recombinant protein fraction according to its expected size on an AKTA Ettan LC FPLC System (Amersham Pharmacy Biotech ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of bEV was measured with Qubit™ Protein and Protein Broad Range (BR) Assay Kits (Thermo Fisher Scientific, Waltham, MA, USA). The expression level of EV markers including CD9 ...
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bioRxiv - Physiology 2024Quote: ... An aliquot of protein was diluted 1:5 in Milli-Q water and protein concentration was quantified using a Pierce™ BCA Protein Assay kit (Thermo Scientific, USA) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... in at least six replicates and raw data were normalized to DNA content measured using the CyQUANT Cell Proliferation Assay Kit (Thermo Fisher Scientific, #C7026).
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bioRxiv - Microbiology 2024Quote: ... PCR amplicons were purified using Vazyme VAHTSTM DNA Clean Beads (Vazyme, Nanjing, China) and quantified with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA, USA). Following quantification ...
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bioRxiv - Systems Biology 2024Quote: ... RNA concentration and quality were assessed using a QUBIT RNA assay kit and a Qubit 4 Fluorometer (both from Invitrogen-Thermo Fisher Scientific Inc.) and an Agilent RNA 6000 Nano kit and an Agilent 2100 Bioanalyzer (both from Agilent Technologies Italia S.p.A.) ...
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bioRxiv - Immunology 2024Quote: ... their kidneys and colon tissues were rapidly excised and homogenized in RIPA lysis buffer after protein concentration was measured with a Pierce™ BCA protein assay kit (23225, Thermo Fisher Scientific). Equal amounts of protein (40 μg for each tissue lysate ...
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bioRxiv - Genomics 2024Quote: ... The DNA quantity and quality were assessed using the Qubit dsDNA High Sensitivity Assay Kit on the Qubit 4 fluorometer (Thermo Scientific, MA, USA) and agarose gel electrophoresis ...
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bioRxiv - Synthetic Biology 2024Quote: About 10µL supernatant was stored at −80°C or directly used for protein quantification with a Pierce Rapid Gold BCA Protein Assay kit (Thermo Scientific, Cat# A53225) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The protein concentrations were determined in the resulting lysates using the Qubit™ Protein and Protein Broad Range (BR) Assay Kit and a Qubit (Thermo Fisher Scientific) fluorimeter.
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bioRxiv - Cell Biology 2024Quote: ... the measured concentrations of various lipid classes (nmol/μL) were divided by protein concentration (μg/μL) that was assessed using BCA Protein Assay Kit (Thermo Fisher Scientific, 23225).
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bioRxiv - Molecular Biology 2024Quote: ... using Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection with Alexa Fluor™ 594 dye (Invitrogen, Catalog number: C10618), as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The gradient fraction at the interface between the 23% and 10% Percoll containing synaptosomes were collected and protein concentration determined by Pierce Bradford Protein Assay Kit (Thermo Fisher Scientific, 23200).
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bioRxiv - Neuroscience 2024Quote: ... Raw cytokine values per well were normalized over nuclei number per well based on fluorescent staining of the fixed iAstrocytes in the plate or over protein content per well determined by Pierce™ BCA protein assay kit (Thermo Scientific, 23225). Pierce™ BCA protein assay was performed in a microplate as described in the user guide provided by Thermo Scientific.
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bioRxiv - Biochemistry 2024Quote: ... centrifuged at 16,000 x g for 15 minutes at 4°C and protein content determined by Pierce BCA protein assay kit (Thermo Fisher Scientific 23225). 25µg total lysate was removed as input and 0.5mg total protein was incubated with pre-washed magnetic M2 anti-FLAG (Sigma-Aldrich M8823-5ML ...
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bioRxiv - Immunology 2024Quote: ... The acid-extracted histones were collected in the supernatant fraction after centrifugation at 650g for 10 min at 4 °C and were neutralized with 2 M NaOH at 1/10 the volume of the supernatant before quantifying protein concentration using BCA® Protein Assay kit (Thermo Scientific #23225)139 ...
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bioRxiv - Genomics 2024Quote: ... Concentration and final size distribution of the library were evaluated using a Qubit 1X dsDNA HS Assay Kit (Thermo Fisher, Waltham, MA, USA) and Femto Pulse System (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of each DNA sample was determined using a high sensitivity Quant-iT™ dsDNA Assay Kit and a Qubit Fluorometer (Thermo Fisher Scientific). Purified ChIP DNA (10 µg ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... RNA concentration was determined using a Qubit 4.0 fluorometer and Qubit™ RNA HS Assay Kit (Cat#Q32852, Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Extracted DNA was quantified in a Qubit 4 fluorometer using the dsDNA HS and the dsDNA BR assay kits (Thermo Scientific, MA, USA). Additional quality evaluation included NanoDrop 2000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Physiology 2024Quote: ... The supernatants containing extracted proteins were recovered and total protein content was quantified by Pierce™ BCA Protein Assay Kits (Thermo Fisher Scientific).
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bioRxiv - Physiology 2024Quote: ... Subsequent lysates were processed for total protein using a commercially available BCA protein assay kit (Thermo Fisher, Waltham, MA, USA; Cat. No. A55864) and spectrophotometer (Agilent Biotek Synergy H1 hybrid reader ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration in all the fractions was measured with the Pierce™ BCA Protein Assay Kit (Cat. number 23225, Pierce, Thermo Fisher Scientific); 20 µg of protein was used for immunoblotting ...
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bioRxiv - Plant Biology 2024Quote: ... was then used in a qRT-PCR duplex assay (Price et al. 2010) with a TaqMan RNA-to-Ct 1-step kit (Applied Biosystems, ThermoFisher Scientific) on a QuantStudio3 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: TUNEL Assay was performed using a Click-iT Plus for In Situ Apoptosis Detection Kit with Alexa Fluor 647 dye (Invitrogen, Cat. No. C10619), according to the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: ... Isolated RNA was quantified using the NanoDrop™ 2000 spectrophotometer and assessed using the Qubit™ RNA IQ Assay Kits (Invitrogen, Cat# Q33221) with the Qubit 4 Fluorometer ...
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bioRxiv - Microbiology 2024Quote: ... DNA integrity was verified using agarose gel electrophoresis and the DNA concentration was determined using QubitTM dsDNA BR assay kit (Thermo Fisher Scientific, Q32853) in combination with the Varioskan LUX plate reader (thermoscientific).
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bioRxiv - Evolutionary Biology 2024Quote: ... Rousettus madagascariensis) underwent additional quantification using an Invitrogen Qubit 3.0 Fluorometer and the Qubit RNA HS Assay Kit (ThermoFisher Scientific, Carlsbad, CA, USA). Following quantification ...
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bioRxiv - Biophysics 2024Quote: ... 100 µL lysate was taken out from the extracts and used to estimate the protein concentration with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). To enrich biotinylated proteins from the protein extracts ...
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bioRxiv - Cancer Biology 2024Quote: ... the antibody was filtered by passing through a 0.22 µm syringe filter followed by estimating the concentration using a Pierce BCA protein assay kit (ThermoFisher Scientific, Waltham, MA, USA). The conjugation reaction was initiated by adding N-hydroxysuccinimide (NHS ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular debris was pelleted for 15min at 4°C and protein concentration was quantitated with the Pierce Bradford Protein Assay Kit (Thermo Fisher Scientific, 23200). A total of 15μg (organoids ...