Labshake search
Citations for Thermo Fisher :
9601 - 9650 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Cell lysates were clarified by centrifugation for 15 min (21.000 x g and 4 °C) and protein content in the supernatant was determined using a Pierce™ BCA assay kit (Thermo Scientific, USA). Cell lysates were diluted to a final concentration of 2 mg protein/ ml in native lysis buffer and kept on ice until used freshly.
-
Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... The number of double-stranded DNA in the genomic DNA samples was quantified by using the Qubit dsDNA HS Assay Kit (Invitrogen, cat no Q32851). Up to 2000 ng of double-stranded genomic DNA was fragmented by Covaris shearing to obtain fragment sizes of 160-180 bp ...
-
bioRxiv - Microbiology 2024Quote: ... The quality and concentration of extracted DNA were determined using a Qubit 4 fluorometer with dsDNA broad range (BR) Assay kit (Thermo Fisher Scientific, Canada). DNA size distribution was confirmed by running a Pippin pulse electrophoresis (Sage science ...
-
bioRxiv - Genetics 2024Quote: ... 1:10 dilution of whole cell lysates in RIPA were used to quantify protein content using Pierce BCA Assay Kit (Thermo Fisher Scientific #23227). 20 μg protein was loaded per sample into 15-well Novex WedgeWell Tris-Glycine Mini gels (Invitrogen #XP08165BOX) ...
-
bioRxiv - Genetics 2024Quote: ... 1:10 dilution of lysates in LP2 buffer were used to quantify protein content using Pierce BCA Assay Kit (Thermo Fisher Scientific #23227). The resulting 2′3′-cGAMP levels were normalized to protein content in each sample.
-
bioRxiv - Microbiology 2024Quote: ... DNA was eluted in 80 μl elution buffer and the concentration was measured using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, USA).
-
bioRxiv - Microbiology 2023Quote: ... DNA was eluted into 10mM Tris and quantified using the Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kit (Thermo Fisher Scientific, UK) using the Qubit™ fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... by washing in phosphate buffer saline (PBS) and storing films at −80 °C for DNA quantification using Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher Scientific), per manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Sample concentrations were quantified based on the standard curve and normalized to total protein obtained through BCA protein assay kit (Thermo Fisher Scientific, 23225) We performed 5-6 independent experiments with 2-3 technical replicates per experiment that were averaged within each experiment.
-
bioRxiv - Molecular Biology 2022Quote: ... two independent IPs were carried out using 120 μg of Arabidopsis chromatin mixed with 3 % of Drosophila chromatin quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). DNA samples eluted and purified from the two technical replicates were pooled before library preparation (Illumina TruSeq ChIP ...
-
bioRxiv - Molecular Biology 2022Quote: ... Then cells were fixed and clicked with Alexa Fluor 488 dye according to manufacturer’s instruction of Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (ThermoFisher Scientific, C10428). Samples were imaged on a Leica SP8 laser scanning confocal microscope at University of Chicago ...
-
bioRxiv - Microbiology 2022Quote: ... Estimation of protein purity and quantity were achieved with SDS-PAGE and a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, IL), respectively.
-
bioRxiv - Microbiology 2023Quote: ... Final libraries were assessed for size and quantity using an Agilent DNA1000 chip and Qubit dsDNA HS Assay Kit (Invitrogen, Carlsbad, California, USA), respectively ...
-
bioRxiv - Immunology 2023Quote: The concentration of total protein in each nasal swab was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher, Cat No. 23225) according to the manufacturer’s microplate procedure ...
-
bioRxiv - Immunology 2023Quote: ... The protein concentrations of the obtained OMVs and OMPs were quantified using a bicinchoninic acid (BCA) assay kit (Thermo Fisher, Rockford, IL, USA) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2023Quote: ... the quantity of DNA was evaluated using a Qubit fluorometer and Qubit DNA BR Assay Kit in a Qubit® 3.0 Fluorometer (Thermo Fisher Scientific, USA). The final DNA concentration was standardized directly prior to library preparation.
-
bioRxiv - Synthetic Biology 2023Quote: ... and the pull-down assay follows the protocol provided by Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, Waltham, MA). The fluorescence intensity (excitation = 485 ...
-
bioRxiv - Plant Biology 2023Quote: ... library concentration and quality were assessed by fluorometry using a Qubit™ 4 and Qubit™ 1X dsDNA HS Assay Kit (ThermoFisher Scientific) and by electrophoresis using a TapeStation (Agilent ...
-
bioRxiv - Physiology 2023Quote: ... LDH was measured on aliquots of the +NaCl-treated cellular supernatant using the CyQUANT™ LDH Cytotoxicity Assay Kit (Thermo Fisher; Cat. #C20300). LDH activity was measured by measuring absorbance at 490 nm ...
-
bioRxiv - Bioengineering 2023Quote: ... The resulting PCR products were gel purified and quantified with Nanodrop and Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851). Pooled guide libraries were sequenced on Illumina NextSeq ...
-
bioRxiv - Physiology 2023Quote: ... low glucose DMEM with heat-killed FITC-labeled Escherichia coli at 250 µg/ml (strain K-12; reagents from Vybrant phagocytosis assay kit; ThermoFisher Scientific; Cat # E2861) (ThermoFisher Scientific ...
-
bioRxiv - Plant Biology 2023Quote: ... quantified internally and externally by University of Minnesota Genomics Center (UMGC) with the Quant-iT RiboGreen RNA Assay Kit (Thermo Fisher, Cat # R11490), and quality checked with the Agilent 2100 Bioanalyzer ...
-
bioRxiv - Plant Biology 2023Quote: ... Final amplification products were purified with the Omega BioTek E-Z 96 Cycle Pure kit and quantified using a Qubit 2.0 fluorometer with the Qubit dsDNA HS assay (Thermo Scientific, Grand Island, NY). DNA was pooled at equal concentration and sent for Illumina MiSeq sequencing at the University of Wisconsin Biotechnology Center (Madison ...
-
bioRxiv - Bioengineering 2023Quote: The cellular content of the various ECM digests was determined using a Quant-iTTM PicoGreen dsDNA assay kit (Invitrogen, Fisher Scientific, Paisley, UK). Following the manufacturers’ protocol ...
-
bioRxiv - Bioengineering 2023Quote: The cellular content of the various ECM digests was determined using a Quant-iTTM PicoGreen dsDNA assay kit (Invitrogen, Fisher Scientific, Paisley, UK). Following the manufacturers’ protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... Residual dsDNA in dPPM and native digests was quantified using the Quant-iT™ PicoGreen™ dsDNA assay kit (Thermo Fisher Scientific, USA) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The concentration of isolated RNA was measured by using Qubit™ microRNA Assay Kit in a Qubit 4 Fluorometer (Thermo Fisher, Waltham, MA).
-
bioRxiv - Microbiology 2023Quote: ... Concentration of the libraries was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Germany). For sequencing the MiSeq instrument (Illumina ...
-
bioRxiv - Immunology 2023Quote: ... Total protein concentrations of 24-hour GD18 urine was determined using a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA) per manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... the lysates were centrifuged for 15 min at 16,000 g and the supernatant containing the proteins were subjected for protein determination using a commercial Bicinchoninic acid assay (BCA) kit (Thermo Fisher, Karlsruhe, Germany). 50 g of proteins were heat desaturated in SDS-sample buffer (supp ...
-
bioRxiv - Microbiology 2023Quote: ... The final purified DNA was stored at -80°C and the DNA concentration was determined using Qubit HS Assay Kit (Invitrogen, Carlsbad, California, USA) on the Qubit 4 Fluorometric Quantification device (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... DNA concentration was assessed using the Qubit® dsDNA HS assay kit on a Qubit® 3.0 fluorometer (Life Technologies, Eugene, Oregon, USA). To rule out contamination with non-14SM members ...
-
bioRxiv - Immunology 2023Quote: ... The total isolated protein from splenic CD4+CD44+ cells was quantified using Bicinchoninic acid (BCA) Protein Assay Kit (Pierce, Thermo Scientific, USA, #23225).
-
bioRxiv - Biochemistry 2023Quote: ... The concentrations of recombinant proteins were determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Cat#A53225, Thermo Fisher Scientific, Rockford, USA).
-
bioRxiv - Bioengineering 2023Quote: ... by washing in phosphate buffered saline (PBS) and storing films at −80 °C for DNA quantification using Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher Scientific), per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... The lysates were cleared by centrifugation at 18,000 x g and 4°C for 20 min and protein concentration was determined using the BCA Protein Assay Kit from Pierce™ (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... EVs from non-treated iRBCs and EVs from CQ-treated iRBCs were added containing 300 μg of protein which was calculated using a BCA assay kit (Thermo Fisher Scientific, USA). 1x PBS was used as the control without EVs ...
-
bioRxiv - Genetics 2023Quote: ... and total protein concentration was estimated using Pierce Bicinchoninic Acid (BCA) protein assay kit according to the manufacturer’s protocol (cat. 23225, Thermo Fisher Scientific, IL, USA).
-
bioRxiv - Evolutionary Biology 2023Quote: ... each IgG was quantified using the Pierce™ BCA Protein Assay Kit as per the manufacturer’s recommendations (Thermo Fisher Scientific, cat. no. 23225).
-
bioRxiv - Physiology 2023Quote: ... the tissues were lyophilized to estimate the ratios of ng DNA per mg dry tissue in each group using the Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Carlsbad, CA, USA) and a SpectraMax M Series Multi-Mode Microplate Reader (Molecular Devices ...
-
bioRxiv - Evolutionary Biology 2023Quote: ATP levels were quantified in cell lysates using a luciferin/luciferase bioluminescence assay (ATP determination kit, Molecular Probes cat # A22066, Thermo Fisher Scientific) as previously described (Jara et al ...
-
bioRxiv - Evolutionary Biology 2023Quote: ATP levels were quantified in cell lysates using a luciferin/luciferase bioluminescence assay (ATP determination kit, Molecular Probes cat # A22066, Thermo Fisher Scientific) as previously described (Jara et al ...
-
bioRxiv - Biochemistry 2023Quote: α-synuclein was quantified in the striatum region of mouse brain utilizing the Enzyme-linked Immunosorbent Assay (ELISA) Kit from Invitrogen (Cat No. KHB0061) following the manufacturer‘s instructions.
-
bioRxiv - Cancer Biology 2023Quote: ... we quantified the cDNA concentration of a subset of samples using th eQuant-iT Picogreen dsDNA Assay kit (Thermo Fisher, Waltham, MA, USA). Standards ranging from 25pg/mL to 25ng/mL were prepared using the Lambda Standard DNA (100ug/mL ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... DNA quality and concentration were estimated with NanoDrop One C spectrophotometer and Qubit 4MT with a dsDNA HS Assay Kit (Invitrogen, Carlsbad, CA, USA). Total of 83 Lonsdalea quercina strains (one strain per tree ...
-
bioRxiv - Bioengineering 2023Quote: ... the supernatants were transferred to clean tubes and their protein concentrations were measured using a BCA Protein Assay Kit (Pierce Thermo Scientific, MA, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... for final quality assurance the CFFT Lab subjected all its plasmids from the daughter plate to next generation sequencing (NGS): DNA from plasmid constructs was quantitated using Qubit dsDNA HS Assay Kit (Q33230, Thermo Fisher Scientific, USA) as per manufacturer instructions ...
-
bioRxiv - Physiology 2023Quote: ... The purity of RNA was examined with the NanoPhotometer® spectrophotometer (IMPLEN) and quantity was determined by Qubit® RNA Assay Kit in Qubit®2.0 Flurometer (Life Technologies). RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies ...
-
bioRxiv - Microbiology 2023Quote: ... DNase-treated RNA was quantified with a Qubit 4 Fluorometer and the Qubit RNA Broad Range Assay Kit per manufacturer’s instructions (ThermoFisher Scientific, Cat. No. Q10211). 1 µg of RNA was then converted to cDNA per manufacturer’s instructions and diluted to a working stock of 5 ng/µL (SuperScript IV VILO Master Mix ...
-
bioRxiv - Microbiology 2023Quote: ... Final RNA concentrations were measured using the Qubit® 2.0 Fluorometer with RNA High Sensitivity (HS) Assay Kit (Life Technologies Corporation, Carlsbad, CA) at the completion of the purification ...