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9851 - 9900 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020

• The hypermorphic PLCγ2 S707Y variant dysregulates microglial cell function – insight into PLCγ2 activation in brain health and disease, and opportunities for therapeutic modulation

  Daniel Bull, et al., bioRxiv - Neuroscience 2023

  Quote: ... was diluted in HBSS assay buffer (Gibco, HBSS supplemented with 1.4mM MgCl2 ...
• Transcriptional memory is conferred by combined heritable maintenance and local removal of selective chromatin modifications

  Pawel Mikulski, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Qubit BR dsDNA assays (Thermo Fisher Scientific) and Real-Time quantitative PCR (RT-qPCR ...
• Temperature elevation synergises with and enhances the type-I IFN-mediated restriction of MPXV

  Chris Davis, et al., bioRxiv - Microbiology 2023

  Quote: ... Qubit RNA HS Assay (Life Technologies, Q32855) and dsDNA HS Assay Kits (ThermoFisher ...
• Angiotensin II type 1 receptor activation facilitates pain hypersensitivity via dorsal horn pericyte mediated vasoconstriction

  Lydia D. Hardowar, et al., bioRxiv - Neuroscience 2023

  Quote: ... Fluo-4-Direct assay (ThermoFisher Scientific; USA) was utilised to measure intracellular calcium concentration in isolated mouse spinal cord pericytes plated in a 96 well plate ...
• In vivo mapping of protein-protein interactions of schizophrenia risk factors generates an interconnected disease network

  Daniel B. McClatchy, et al., bioRxiv - Neuroscience 2023

  Quote: ... and a BCA protein assay (Fisher Scientific) was performed ...
• Loss of intracerebellar heterogeneity and selective vulnerability in Spinocerebellar ataxia type 1 neurodegeneration

  Katherine Hamel, et al., bioRxiv - Neuroscience 2023

  Quote: ... including fluorometric quantification (RiboGreen assay, Life Technologies) and RNA integrity with capillary electrophoresis (Agilent BioAnalyzer 2100 ...
• Variants in the DDX6-CXCR5 autoimmune disease risk locus influence the regulatory network in immune cells and salivary gland

  Mandi M. Wiley, et al., bioRxiv - Genetics 2023

  Quote: ... and rs4938572 (ThermoFisher, #4351379, Assay ID#C_28012712_10) (Supplemental Table 1) ...
• Th2 and Th17-Associated Immunopathology Following SARS-CoV-2 Breakthrough Infection in Spike-Vaccinated ACE2-humanized Mice

  Tianyi Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... and Il10 (Mm01288386_m1) (Applied Biosystems TaqMan Assays) were used as per manufacturer’s instructions ...
• Human monoclonal antibodies reveal subdominant gonococcal and meningococcal cross-protective antigens

  Marco Troisi, et al., bioRxiv - Immunology 2023

  Quote: ... quantified by Qubit Fluorometric Quantitation assay (Invitrogen) and used for transient transfection in Expi293F cell line (Thermo Fisher Scientific ...
• Kinome focused CRISPR-Cas9 screens in African ancestry patient-derived breast cancer organoids identifies essential kinases and synergy of EGFR and FGFR1 inhibition

  Florencia P. Madorsky Rowdo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and the Oncomine assay developed by ThermoFisher Scientific ...
• Characterisation of TbSmee1 indicates that endocytosis is required for access of surface-bound cargo to the trypanosome flagellar pocket

  Daja Schichler, et al., bioRxiv - Cell Biology 2023

  Quote: ... Assays using BSA (AlexaFluor555 conjugate, Molecular Probes) were done in the same way ...
• WNK1-dependent water influx is required for CD4⁺ T cell activation and T cell-dependent antibody responses

  Joshua Biggs O’May, et al., bioRxiv - Immunology 2023

  Quote: ... TaqmanTM Gene Expression Assays (Thermo Fisher Scientific) were used to measure Wnk1 (exon boundary 1-2 ...
• Trans-omic analysis reveals opposite metabolic dysregulation between feeding and fasting in liver associated with obesity

  Yunfan Bai, et al., bioRxiv - Systems Biology 2023

  Quote: ... bicinchoninic acid (BCA) assay (Thermo Fisher Scientific) was used to quantify the protein concentration of the lysates ...
• In Vivo Biorthogonal Antibody Click for Dual Targeting and Augmented Efficacy in Cancer Treatment

  Sandeep Surendra Panikar, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Pierce 660 assay (Thermo Fisher Scientific, 22660), or BCA assay (Thermo Fisher Scientific ...
• Actin-dependent recruitment of AGO2 to the zonula adherens

  Mary Catherine Bridges, et al., bioRxiv - Cell Biology 2023

  Quote: ... TaqMan assays used (Applied Biosystems, cat# 4427975): hsa-miR-24 ...
• Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high density growth

  Max G. Schubert, et al., bioRxiv - Microbiology 2023

  Quote: ... broad range assay (ThermoFisher Scientific, Cat. # Q33265). Long read sequencing was performed by Plasmidsaurus (Eugene ...
• Microcosm cultures of a complex synthetic community reveal ecology and genetics of gut microbial organization

  Xiaofan Jin, et al., bioRxiv - Microbiology 2022

  Quote: ... using the Quant-iT PicoGreen assay (ThermoFisher). To generate input DNA for our high-thoughput and low-volume Nextera XT library preparation process ...
• Inducible co-stimulatory molecule (ICOS) alleviates Paclitaxel induced peripheral neuropathy via an IL-10-mediated mechanism in female mice

  Ishwarya Sankaranarayanan, et al., bioRxiv - Neuroscience 2022

  Quote: ... Pierce BCA Protein Assay (Thermo Fisher Scientific) was performed by following the manufacturer’s protocol to identify the amount of protein in each sample ...
• Multiplex HDR for Disease and Correction Modeling of SCID by CRISPR Genome Editing in Human HSPCs

  Ortal Iancu, et al., bioRxiv - Bioengineering 2022

  Quote: ... RNase P amplification (TaqMan assay, Applied Biosystems) was conducted for quality control of the DNA TREC amplification.
• High-throughput microgel biofabrication via air-assisted co-axial jetting for cell encapsulation, 3D bioprinting, and scaffolding applications

  Vaibhav Pal, et al., bioRxiv - Bioengineering 2022

  Quote: ... Alamar blue dye reduction assay (Invitrogen, USA) was used following the manufacturer’s protocol ...
• Elevated FOXG1 supports exit from quiescence in neural stem cells through FoxO6

  Kirsty M Ferguson, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and TaqMan gene expression assays (Life Technologies) on a QuantStudio™7 Flex Real-Time PCR machine ...
• Modulation of fibroblasts phenotype by colorectal cancer cells-secreted factors is mostly independent of oncogenic KRAS

  Patrícia Dias Carvalho, et al., bioRxiv - Cancer Biology 2022

  Quote: ... TaqMan Gene Expression Assays (Thermo Fisher Scientific) or PrimeTime qPCR Assays (Integrated DNA Technologies ...
• ASCL1 interacts with the mSWI/SNF at distal regulatory elements to regulate neural differentiation

  Oana Păun, et al., bioRxiv - Developmental Biology 2022

  Quote: ... and QubitTM dsDNA HS assay (Life Technologies), respectively ...
• Substrate stiffness engineered to replicate disease conditions influence senescence and fibrotic responses in primary lung fibroblasts

  Kaj E.C. Blokland, et al., bioRxiv - Cell Biology 2022

  Quote: ... and TaqMan Gene Expression Assay (Thermo Scientific) on a ViiA7 Real-Time PCR System (Applied Biosystems ...
• Schlafen 12 restricts HIV-1 latency reversal by a codon-usage dependent post-transcriptional block in CD4+ T cells

  Mie Kobayashi-Ishihara, et al., bioRxiv - Immunology 2022

  Quote: ... and TaqMan gene expression assay (Applied Biosystems) was used for detection of SLFN12 (Hs00430118_m1 ...
• Parsing the role of NSP1 in SARS-CoV-2 infection

  Tal Fisher, et al., bioRxiv - Microbiology 2022

  Quote: The OPP assay (OPP, Thermo Fisher Scientific) was carried out following the manufacturer’s instructions ...
• Contribution of 6mer seed toxicity to HIV-1 induced cytopathicity

  Aparajitha Vaidyanathan, et al., bioRxiv - Microbiology 2022

  Quote: ... or GAPDH (Thermo Scientific Assay ID: Hs00266705_g1) specific TaqMan primer-probe ...
• Feedback between a retinoid-related nuclear receptor and the let-7 microRNAs controls the pace and number of molting cycles in C. elegans

  Ruhi Patel, et al., bioRxiv - Developmental Biology 2022

  Quote: ... we set-up TaqMan assays (ThermoFisher Scientific) in 96-well plates ...
• Preliminary prospective study on the predictive significance of plasma miR-30b-5p through the study of echocardiographic modifications in Cavalier King Charles Spaniels affected by different stages of myxomatous mitral valve disease: the PRIME study

  Sara Ghilardi, et al., bioRxiv - Genomics 2022

  Quote: ... The small RNA TaqMan assays (ThermoFisher Scientific) included cel-miR-39-3p (assay ID 478293_mir ...
• Galectin-9 interacts with Vamp-3 to regulate cytokine secretion in dendritic cells

  Rui Santalla Méndez, et al., bioRxiv - Cell Biology 2023

  Quote: ... The BCA protein assay (Pierce, ThermoFisher scientific) was conducted to determine protein concentration and following manufacturer’s instructions and for each sample ...
• Single-nucleus sequencing reveals enriched expression of genetic risk factors in Extratelencephalic Neurons sensitive to degeneration in ALS

  Francesco Limone, et al., bioRxiv - Neuroscience 2023

  Quote: ... After protein quantification by BCA assay (ThermoFisher), ten micrograms of proteins were preheated in Laemmli’s buffer (BioRad) ...
• Foreign body reaction is triggered in vivo by cellular mechanosensing of implants stiffer than host tissue

  Alejandro Carnicer-Lombarte, et al., bioRxiv - Bioengineering 2022

  Quote: ... A bicinchoninic acid (BCA) assay (ThermoFisher, 23225) was used to quantify protein content following manufacturer specifications ...
• Aberrant miR-29 is a predictive feature of severe phenotypes in pediatric Crohn’s disease

  Alexandria J. Shumway, et al., bioRxiv - Genomics 2022

  Quote: ... and Sis (Life Technologies, Assay ID: Mm01210305_m1). Any undetectable samples were not included in the analysis.
• MicroRNA-eQTLs in the developing human neocortex link miR-4707-3p expression to brain size

  Michael J. Lafferty, et al., bioRxiv - Genetics 2023

  Quote: ... The Clariom S Pico Assay (ThermoFisher 902963) microarray was used to quantify sample transcriptomes ...
• Identifying transcriptomic downstream targets of genes commonly mutated in Hereditary Hemorrhagic Telangiectasia

  Md Khadem Ali, et al., bioRxiv - Cell Biology 2022

  Quote: MTT assay (Cat # V13154, Invitrogen, Waltham, MA), and caspase-3/7 assay (Cat # G8090 ...
• Interferon restores replication fork stability and cell viability in BRCA-defective cells via ISG15

  Uddipta Biswas, et al., bioRxiv - Cancer Biology 2023

  Quote: TaqMan gene expression assays (Thermo Fisher Scientific) for Trim25-/- and Ube1L-/- MEFs were performed ...
• Senescent response in inner annulus fibrosus cells in response to TNFα, H₂O₂, and TNFα-induced nucleus pulposus senescent secretome

  Aaryn Montgomery-Song, et al., bioRxiv - Cell Biology 2022

  Quote: For the Live/Dead assay (Invitrogen, L3224), cells were washed in DMEM and treated with Calcein-AM (2 μM ...
• Mutational signatures in wild type Escherichia coli strains reveal dominance of DNA polymerase errors

  Sofya K. Garushyants, et al., bioRxiv - Evolutionary Biology 2023

  Quote: ... quantified it (Qubit HS dsDNA assay, Invitrogen), and pooled equal amounts of genomic DNA from each of the 20 lines for a given mutator strain into a single tube ...
• Single-cell mRNA-regulation analysis reveals cell type-specific mechanisms of type 2 diabetes

  Jose Martinez-Lopez, et al., bioRxiv - Cell Biology 2023

  Quote: ... All TaqMan assays were from Life Technologies and qPCR reactions were run on the Viia7 real-time PCR system (Applied Biosystems ...
• A subset of viruses thrives following microbial resuscitation during rewetting of a seasonally dry California grassland soil

  Alexa M. Nicolas, et al., bioRxiv - Microbiology 2023

  Quote: ... amount of DNA (PicoGreen assay (Life Technologies)) and community composition (in this case shotgun metagenomics) ...
• Nucleocytoplasmic transport of active HER2 causes fractional escape from the DCIS-like state

  Lixin Wang, et al., bioRxiv - Systems Biology 2023

  Quote: ... and TaqMan MicroRNA Assays (Thermo Fisher, 4427975) for MIR205 (Assay 000509 ...
• Amphiphilic particle-stabilized nanoliter droplet reactors with a multi-modal portable reader for distributive biomarker quantification

  Vishwesh Shah, et al., bioRxiv - Bioengineering 2023

  Quote: ... we added a QuantaRed assay solution (ThermoFisher) to the particles ...
• Drosophila AHR limits tumor growth and stem cell proliferation in the intestine

  Minghua Tsai, et al., bioRxiv - Cancer Biology 2023

  Quote: ... using TaqMan Gene Expression Assays (Applied Biosystems), universal PCR Master Mix (Applied Biosystems ...
• Sex-specific regulation of the cardiac transcriptome by the protein phosphatase 2A regulatory subunit B55α

  Nicola M Sergienko, et al., bioRxiv - Physiology 2024

  Quote: ... Taqman gene expression assays from Thermo Fisher Scientieic (Waltham ...
• A broad cathepsin inhibitor blocks crystal-stimulated inflammasome-dependent and -independent inflammation, and gout arthritis

  Laura Alejandra Ariza Orellano, et al., bioRxiv - Immunology 2024

  Quote: ... CyQUANT LDH Cytotoxicity Assay was from Invitrogen. Anti-CD11b (M1-70) ...
• Functional roles of neural aPKCs in mouse brain development and survival

  Aicha El Ellam, et al., bioRxiv - Developmental Biology 2024

  Quote: LanthaScreen TR-FRET Kinase Assay (ThermoFisher Scientific) was used according to manufacturer’s instructions ...
• Commensal bacteria inhibit viral infections via a tryptophan metabolite

  Danting Jiang, et al., bioRxiv - Microbiology 2024

  Quote: ... and TaqMan Gene Expression Assays (Applied Biosystems) for Gapdh (HS02786624_g1) ...
• Adipose microenvironment promotes hypersialylation of ovarian cancer cells

  Alexandra Fox, et al., bioRxiv - Cancer Biology 2024

  Quote: ... St6GalNac3 (Thermo Fisher Scientific Assay ID: Mm01316813_m1); and RPS17(Thermo Fisher Scientific Assay ID ...
• Adipose microenvironment promotes hypersialylation of ovarian cancer cells

  Alexandra Fox, et al., bioRxiv - Cancer Biology 2024

  Quote: ... St6Gal1 (Thermo Fisher Scientific Assay ID: Mm00486119_m1); St6GalNac3 (Thermo Fisher Scientific Assay ID ...
• Amniotic fluid stem cell extracellular vesicles promote lung development via TGF-beta modulation in a fetal rat model of oligohydramnios

  Fabian Doktor, et al., bioRxiv - Developmental Biology 2024

  Quote: ... A bicinchoninic acid assay (Thermo Fisher Scientific) was used for quantification of proteins ...