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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... TOGARAM1 was depleted using siRNA with sequence 5′-CCUCGUAAUUCCUUAGAAA-3′ (Thermo Scientific) Cells were seeded onto coverslips at 70% confluency and transfected with 50 nM of siRNA in two sequential transfections using Lipofectamine RNAiMAX (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... ID: s2513 (Silencer Select Validated; 5’-GA UAUACCCUGGAAAGUCUtt-3’) (Thermo Fisher Scientific) with JetPrime (Polyplus ...
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bioRxiv - Neuroscience 2021Quote: ... cells were permeabilized with saponin-based permeabilization and wash reagent (Invitrogen, Click-iT Plus EdU Kit) for 15 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... TaqMan based RT-qPCRs were implemented using the AG-Path-One Step RT-PCR Kit (Ambion). Cycling was performed on a Bio-Rad CFX96 real-time PCR detection system (BioRad ...
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bioRxiv - Genomics 2019Quote: ... DNA purity and quantity were estimated using spectrophotometer and Qubit fluorescent based kit (Thermo Fisher Scientific). DNA integrity was assessed on 0.8% agarose gel electrophoresis.
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bioRxiv - Microbiology 2021Quote: ... Quantification was performed with the fluorescent-based quantitation Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). Sequencing was performed as a Single Read run for 65 bp sequences on HiSeq 2500 Illumina sequencer (65 cycles) ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized using an M-MLV reverse transcriptase-based cDNA first-strand synthesis kit (Invitrogen). qRT-PCR was performed using the SYBR® Green CR Master Mix Kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... The first was a luciferin-based ATP assay (ATP Determination Kit, Life technologies, Eugene, Oregon, USA) as described elsewhere [14] and the second was determined by the value of OCR change following GC30 treatment.
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using biuret reaction-based Pierce BCA Protein Assay kit (Thermo Fisher Scientific). The microplate procedure recommended in the manufacturer’s user guide was followed ...
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bioRxiv - Microbiology 2023Quote: ... Quantification was performed with the fluorescent-based quantitation Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). Sequencing was performed as a Single Read Multiplexed run for 65Lbp sequences on HiSeq 2500 sequencer (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... Quantification was performed with the fluorescent-based quantitation Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). Sequencing was performed as a Single Read run for 75 bp sequences on a NextSeq500 Illumina sequencer ...
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bioRxiv - Bioengineering 2023Quote: ... 16S based libraries were quantified by fluorimetry using QuantliT™ PicoGreen™ dsDNA Assay Kit (Thermofisher).
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bioRxiv - Cancer Biology 2023Quote: A florescence shift-based assay (Image-iT Lipid Peroxidation Kit, Cat. No. C10445, Thermo Fisher Scientific) was used to analyze the peroxidation of lipid in live cells exploiting the BODIPY 581⁄591 C11 fluorescent reporter lipid probe as described previously (35) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the concentration is measured by fluorescence-based quantification (Qubit dsDNA BR Assay Kit, Thermo Scientific). Samples are normalised to be within the 20 – 90 fmol/μL range and 1 μL of sample is used for library preparation ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (RT-qPCR) was performed using SYBR Green dye-based assay in a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) with the following reaction conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... were ordered from Twist Biosciences and cloned with restriction-ligation based approach to the 3’ end of GFP using BcuI (Thermo Fisher Scientific) and BstBI (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... and subjected to SYBR® Green chemistry-based qPCR using the QuantStudio 3 Real-time PCR System (Applied Biosystems, Foster City, CA). Primers were obtained from ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μl of samples were transferred to a microwell plate (Nunc™ 3 84-Well Optical Bottom Plates # 242764, Thermo Scientific) and the time course of fluorescence-intensity (see Fig ...
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bioRxiv - Immunology 2024Quote: High-throughput screening of antibodies was done using Expi293 cells grown in 3 mL plates in 24 deep-well plates (Thermo Fisher). Cultures were grown for four days ...
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bioRxiv - Bioengineering 2022Quote: RPTEC organoids were imaged every 2-3 days with a EVOS FL 2 Auto microscope (Thermo Fisher) with a 4x objective ...
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bioRxiv - Cell Biology 2020Quote: ... The GeneChip 3’ IVT PLUS Reagent Kit (Affymetrix/Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase-3 and −7 Cell Imaging Kit (Invitrogen), and Propidium iodide (PI ...
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bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies). The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 RBD was coated onto Immuno-MaxiSorb plates (Nunc) at final concentration of 2 μg/mL for 2 hr at 4°C ...
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bioRxiv - Immunology 2023Quote: ELISA plates were coated with 2 µg/ml NeutrAvidin (ThermoFisher, 31000) and blocked as described above ...
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bioRxiv - Microbiology 2024Quote: ... Caco-2 cells were seeded into 24-well plates (Thermo Fisher) at a density of 1.5×105 cells/cm2 and grown for 4 days before treatment with TMAO at varied concentrations for the experiment ...
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bioRxiv - Microbiology 2024Quote: Caco-2 cells were seeded into 24-well plates (Thermo Fisher) at a density of 1.5×105 cells/cm2 and grown for 4 days before treatment with TMAO at varied concentrations for the experiment ...
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bioRxiv - Genomics 2020Quote: ... based medium with N2 (17502048, Gibco), B27(17504-044 ...
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bioRxiv - Cell Biology 2022Quote: ... lipofectamine-based transfection (Lipofectamine 3000, ThermoFisher) and ...
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bioRxiv - Microbiology 2021Quote: Plasmid based on pBAD/His (Invitrogen) or pHT01 (MoBiTech ...
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bioRxiv - Neuroscience 2020Quote: ... a Neurobasal-A based medium (Thermofisher) supplemented with 2 % B27 (Thermofisher) ...
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bioRxiv - Biophysics 2022Quote: ... after Lipofectamine 2000-based transfection (Invitrogen).
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was discarded and the pellet was resuspended in 5 mL liquid KNOP supplemented with 2% (w/v) sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418 ...
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bioRxiv - Neuroscience 2022Quote: ... plates were thawed and 5 μL of RNAiMAX (Invitrogen, Carlsbad, CA, USA) diluted in culture media without antibiotics (3% v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µg/ml TcGST were immobilised onto ELISA plates (Maxisorp, Thermofisher Scientific), free binding sites were blocked with Superblock (Thermofisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... qPCR reactions were run on 384-well plate QuantStudio 5 (Applied Biosystems). The corresponding standard curve for absolute quantification was performed using the 199 bp amplicon as opposed to a plasmid containing the amplicon ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... seeded with 2,000 cells/well and stained with Hoescht 33258 (1µg/mL) and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at a dilution of 1000x ...
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bioRxiv - Cancer Biology 2022Quote: ... alongside aforementioned activating proteins and CellEvent™ Caspase-3/7 Green Detection Reagent (1:5000, ThermoFisher Scientific, Cat#C10423). Cells were imaged using the IncuCyte ZOOM System (Essen Bioscience ...
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bioRxiv - Immunology 2022Quote: ... in the presence of T cells at a 1:1 effector:target cell ratio in the presence of the caspase-3/7 Green Detection Reagent (Invitrogen). Images were taken every 2 h at 10× magnification ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cycling was performed using a QuantStudioTM 3-96 well 0.1 mL Block Real time PCR Detection system (ThermoFisher, USA), and transcript quantities were derived by the system software ...
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bioRxiv - Cell Biology 2020Quote: ... 5-ethynyl-2-deoxyuridine (EdU) labelling performed with Invitrogen Click-iT EdU Alexa Fluor 594 Imaging Kit (Life Technologies, C10339).
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bioRxiv - Cancer Biology 2022Quote: ... we performed 5-ethynyl-2′-deoxyuridine (EdU) staining using the Click-iT EdU Alexa Fluor 647 imaging kit (C10340, Invitrogen). Four-color image acquisition of three replicates per condition was performed on an SP8 confocal microscope (Leica) ...
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bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) staining was performed using Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Click-iT Alexa Fluor 488 EdU (5-ethynyl-2’-deoxyuridine) imaging kit and Lipofectamine RNAiMAX was purchased from Life Technologies. Foetal Bovine Serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected pregnant mice intraperitoneally with 100 μL of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Alexa Fluor 647 Imaging Kit, Invitrogen) at 1mg/mL ...
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bioRxiv - Developmental Biology 2022Quote: 5-ethynyl-2-deoxyuridine (EdU) pulse labeling was performed with the Click-IT EdU imaging kit (Life Technologies; Cat#:C10340) as described in (Perillo et al. ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
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bioRxiv - Cell Biology 2023Quote: Cell proliferation was assessed using the 5-Ethynyl-2-deoxyuridine (EdU) incorporation assay (Click-iT EdU Alexa Fluor 488 Imaging Kit, Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: 2 x104 MPI-2 cells/well were seeded in a white clear-bottom 96 well plates (ThermoFisher) and incubated at 37°C with 5% CO2 for 24 hours ...