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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... a selected cell terminal was puffed for 5 s with a solution containing 3-5 μM FM1-43 (Molecular Probes) and (in mM) ...
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bioRxiv - Microbiology 2020Quote: The Q577R gp41 change was introduced into pSHIV-AD8-EO via site-directed mutagenesis using 5’p-TCAAGCAGCTCCGGGCAAGAGTCC-3’ (forward) and 5’p-TGCCCCAGACTGTGAGTTGCAACA (reverse) with Platinum SuperFi PCR mastermix (ThermoFisher) as described in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 x 5 min in PBS and incubated for 5 min in DAPI (Invitrogen, cat# D1306) 1:1000 in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... minced skin was incubated at 37°C for 3 – 5 hours in 5 ml of DMEM high glucose (#41965-039; Gibco) supplemented with 10 mg ml-1 collagenase (#C9891 ...
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bioRxiv - Biochemistry 2023Quote: The ibpA 5’ UTR-gfp mRNA produced from CUGA®7 in vitro transcription kit (Nippon Gene) was attached to a 3’-terminal biotinylated nucleotide using Pierce™ RNA 3’ End Biotinylation kit (Thermo Scientific). The biotin-labeled mRNA (0.1 µM ...
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bioRxiv - Cell Biology 2020Quote: Proliferation was assessed through incorporation of 5-ethynyl-2’-deoxyuridine (EdU) using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen, C10425). One day prior to harvest ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-ethynyl-2′-deoxyuridine (EdU) incorporation was detected using a Click-iT Plus EdU Alexa Fluor 555 Assay Kit (Invitrogen, C10638) prior to primary antibody incubation ...
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bioRxiv - Neuroscience 2019Quote: The study of proliferation and differentiation of neural stem cells in the dentate gyrus was performed by incorporation of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Imaging Kit; Molecular Probes) to allow the analysis of proliferation and differentiation ...
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bioRxiv - Microbiology 2020Quote: ... and purified RNA from 2 ml of cells harvested immediately before and 5 min after stress using the RiboPure™-Bacteria Kit (Ambion), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... and dissociated into single cells in 100 μl of Enzyme 1 and 5 μl of Enzyme 2 (Pierce Cardiomyocytes Dissociation Kit, ThermoFisher Scientific) for 30 min at 350 rpm shaking at 30°C ...
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bioRxiv - Developmental Biology 2019Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) labeling was performed using the Click-iT EdU Alexa Fluor 647 Imaging Kit (Invitrogen Cat#C10340) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... newly synthesized DNA was labeled using the Click-iT Plus 5-ethynyl-2’-deoxyuridine (EdU) Alexa Fluor 647 flow cytometry assay kit (ThermoFisher Scientific) according to manufacturer ‘s instructions ...
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bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed 3 more times in 1x PBS before DNA detection with 1 µg/ml Hoechst 33342 (Invitrogen, H3570) for 15 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (C10723) and PrestoBlue™ Cell Viability Reagent (A13261) were purchased from ThermoFisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with a dye master mix containing CellEventTM Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific, 1:1000) and Zombie NIR fixable viability stain (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was replaced with DMEM + 10% FBS + CellEvent Caspase-3/7 Detection Reagent (Green) according to the manufacturers recommendations (Invitrogen). The plate was then imaged every 15 minutes using phase and GFP channels in the ZEISS Celldiscoverer 7 Automated Live Cell Imager set to 42°C at 5% CO2 and 20% O2 ...
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bioRxiv - Microbiology 2021Quote: ... per well to a 2 mL deep well plate (Life Technologies, #4388476), followed by the addition of either 375 or 750uL of lysate ...
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bioRxiv - Microbiology 2023Quote: ... plates were coated with 2 µg/ml anti-His (Invitrogen #MA1-21315) over night and blocked for 1hour as described above ...
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bioRxiv - Immunology 2023Quote: ... To assess active caspase 3 activity the CaspGLOW™ Fluorescein Active Caspase-3 Staining Kit (Invitrogen) was used ...
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bioRxiv - Neuroscience 2021Quote: ... 3 µg of RNA using SuperScript II Kit (Invitrogen) were reverse transcribed to cDNA ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse TNFα ELISA Kit (Thermo Fisher Scientific, BMS607-3), Mouse IL6 ELISA Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 3 % SDS and 5 mM EDTA) and protein concentrations were determined with a Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... We then used the linearized MLM3613 as template to synthesize the 5′ capped and 3′ polyA-tailed Cas9 mRNA using the mMESSAGE mMACHINE® T7 Ultra Kit (ThermoFisher, cat no: AM1345) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Stably transfected inducible STING-GFP or NET55-GFP HT1080 cells grown on 25 mm round coverslips mounted in an Attofluor incubation chamber (Life Technologies) were first induced for expression of the fusion protein overnight with doxycycline ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used in this study were all anti-human: STING (1:1000, CST, #13647 or 1:500, Thermo Fisher, MA526030), UBIQUITIN (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... We then diluted these saturated cultures 1:20 in sterile water and then inoculated 3 µL into 96-well humidified plates (Nunc Edge Plates (Thermo Scientific, Waltham, MA)) with 150 µL of YPD or SD-CSM per well ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed directly on a plate with 5 ml of TRIzol (Invitrogen), total RNA was isolated following manufacturer’s protocol and sonicated by two pulses of 30 seconds in a Bioruptor instrument ...
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bioRxiv - Biochemistry 2019Quote: ... 5 % CO2 and ambient oxygen level on Geltrex coated plates (Thermo Fisher Scientific) in mTeSR1 medium (StemCell Technologies ...
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bioRxiv - Microbiology 2020Quote: ... HIOs were cultured in groups of 5/well using 4-well plates (ThermoFisher). Individual HIO lumens were microinjected using a glass caliber needle with 1μl of PBS control or different STm mutants (105CFU/HIO or 103CFU/HIO for 24h infections) ...
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bioRxiv - Molecular Biology 2020Quote: 5 × 15cm plates of HEK293 Flp-In T-REx cells (ThermoFisher Scientific, R78007) at 80% confluency were harvested in 15mL DMEM media by trypsinization ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×103 cells were seeded in a 96-well microtiter plate (Thermo Nunc) with fresh 10% serum medium and incubated for 24 hours for their adherence ...
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bioRxiv - Cell Biology 2021Quote: ... the medium was changed to the same RPMI-based medium and supplements except for the replacement of FBS with 2% KOSR (Life Technologies) and the incubation was maintained for another 2 days.
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bioRxiv - Cell Biology 2020Quote: ... Ct values were determined by the software provided with the Mx3000P QPCR System and differences in mRNA expression were calculated by the 2-ΔΔct method [24] based on validation tests performed by Applied Biosystems.
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bioRxiv - Cell Biology 2023Quote: Gene expression of targets listed in supplemental table 2 were analysed by SYBR green-based qPCR analysis using Applied Biosystems QuantStudio 7 Real-Time PCR Systems (Thermo Scientific), following the standard PCR cycling sequence using GAPDH as an internal control ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of RNA of each sample was mixed with 2 µl of ERCC ExFold spike-in RNA mixes based on the RNA ratio recommended by the protocol (Invitrogen 4456739), rRNA were depleted by hybridization of complementary DNA oligonucleotides and treatment with RNase and DNase ...
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bioRxiv - Cell Biology 2024Quote: ... and these values were re-calibrated based on measurements of NIST-certified polystyrene beads (2 µm and 6 µm in diameter, Duke Standards, Thermo Scientific).
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bioRxiv - Neuroscience 2022Quote: ... The membrane was exposed using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher).
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor detection was performed according to Click-iT EdU Fluor Imaging Kit (Invitrogen)
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bioRxiv - Biochemistry 2020Quote: DNA and RNA were quantified using DNA/RNA specific fluorescent detection kits (Qubit, Invitrogen). Total protein was measured using BCA detection kit (Pierce).
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bioRxiv - Plant Biology 2021Quote: ... SuperSignal West Dura Detection Kit was used to detect anti-tubulin (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... then analyzed for viability using the FITC Annexin V Apoptosis Detection Kit (Thermo Scientific). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... and tested negative for mycoplasma contamination using the Cell Culture Contamination Detection Kit (ThermoFisher).
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bioRxiv - Biochemistry 2021Quote: ... The results were detected using a chemiluminescent nucleic acid detection module kit (Thermo Scientific) and scanned by ChemiDoc™ Touch Imaging System and quantified by ImageJ ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA detection was done using a Click-iT RNA Alexa Fluor Imaging kit (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... TRAP staining was performed using the ELF-97 endogenous phosphatase detection kit (Thermo Scientific). A Zeiss LSM800 confocal microscope was used for fluorescent imaging at the BWH Confocal Microscopy Core.
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bioRxiv - Microbiology 2020Quote: ... Peroxidase activity was visualized with an enhanced chemiluminescence detection kit (Thermo Scientific, Bremen, Germany) on ChemiDoc MP Imaging System (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... EdU detection was done following the manufacturer’s instruction (Click-iT EdU Imaging kit, Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... the detection of proteins was performed by using enhanced chemiluminescence kit (32106, Thermo Scientific) and the image was captured by a LI-COR Odyssey Fc Imager system ...
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bioRxiv - Molecular Biology 2023Quote: ... Signals were visualized with a Biotin Chromogenic Detection Kit (Thermo Scientific, Waltham, MA, USA).