Labshake search
Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 2' 3' cGAMP STING based FRET Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Detection was then done using Pierce ECL Plus Chemiluminescence kit (Thermo Fisher Scientific, 32106). Imaging was then done using Bio-Rad ChemiDoc MP system.
-
bioRxiv - Cell Biology 2023Quote: ... Chemiluminescent detection was conducted using either SuperSignal West Pico or Femto kits (Thermo Scientific) after 45-minute incubation with secondary antibodies listed in Supplemental Methods at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was then determined using a Pierce BCA protein detection kit (Thermo Scientific) and samples were normalised to 2 µg/1ul ...
-
bioRxiv - Bioengineering 2023Quote: Cells were processed as follows using the GeneArt Genomic Cleavage Detection Kit (ThermoFisher #A24372). Cell media was collected in a 1.5 mL Eppendorf tube ...
-
bioRxiv - Biochemistry 2023Quote: ... Genomic cleavage by Cas9 was confirmed using GeneArt Genomic Cleavage Detection kit (Life Technologies) to amplify the region of genomic DNA targeted for cleavage ...
-
bioRxiv - Biochemistry 2023Quote: ... according to the protocol prepared for Chemiluminescent Nucleic Acid Detection Module kit (Thermo Scientific). To examine the interaction of IbpAEc-WT with the mRNA in the presence of IbpAEc-R93A ...
-
bioRxiv - Microbiology 2023Quote: ... and the Detection Kit Genome amplification (Thermo Fisher Scientific, internal control 25-30 Ct). Positive samples were tested to determine the Influenza A subtype H5 lineage (specific 2.3.4.4 clade ...
-
bioRxiv - Neuroscience 2023Quote: ... The glucose concentration was subsequently determined using a Glucose Colorimetric Detection Kit (Invitrogen, #EIAGLUC). The assay results were read using a Hidex Sense Microplate Reader.
-
bioRxiv - Cancer Biology 2024Quote: ... The apoptosis was measured by eBioscienc Annexin V apoptosis detection kit FITC (Thermo Fisher). Flow cytometry analysis was performed using an Amnis FlowSight flow cytometer (Millipore).
-
bioRxiv - Microbiology 2024Quote: ... then chemiluminescence was detected using a Chemiluminescent Nucleic Acid Detection Module kit (Thermo Scientific) by exposure on a Tanon-5200 Multi instrument (Tanon Science & Technology Co ...
-
bioRxiv - Biophysics 2022Quote: ... Protein was liquated to 3 replicate wells of a 384-well–plate (Thermo Scientific Nunc), and plates were incubated at 37 °C for 60 h with shaking ...
-
bioRxiv - Biophysics 2020Quote: ... Cells seeded on a 35mm plate were stained with Fluo-3 AM calcium indicator (Thermofisher) at 1 µM for 20 minutes incubation after which seeded cells were image on the microscope using 488nm excitation.
-
bioRxiv - Biophysics 2022Quote: ... Protein was liquated to 3 replicate wells of a 384-well–plate (Thermo Scientific Nunc), and plates were incubated at 37 °C for 60 h with shaking ...
-
bioRxiv - Bioengineering 2023Quote: ... The plates were washed 3 times again before adding anti-hIgG-HRP (Invitrogen, Cat # A18823) antibody for detection ...
-
bioRxiv - Microbiology 2023Quote: ... A549 cells were seeded in 96-well microtiter plates at a concentration of 5−104 cells per well for 24 h (37°C, 5% CO2) in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
-
bioRxiv - Neuroscience 2021Quote: ... After washing again for 3x 5 min in PBS bands were visualized with luminol-based enhanced chemiluminescence HRP substrate (Super signal west dura, Thermo Fisher Scientific) and the ImageQuant LAS 4000 system (GE healthcare ...
-
bioRxiv - Genomics 2022Quote: ... Clump-based cell splitting was performed by incubating the cells in final 5 mM EDTA pH 8,0 (Thermo Fisher Scientific, 15575-038) in DPBS (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... Loveton Circle Sparks, MD, USA) supplemented with 5% fetal bovine serum (FBS; CLARK, USA) or on Columbia-based blood agar media (ThermoFisher Scientific, China) at 37 °C ...
-
bioRxiv - Biochemistry 2019Quote: Horseradish peroxidase (HRP)-coupled Amplex red assay was performed on SpectraMax M2 multi-detection microplate reader using black 96-well plate with clear bottom from Nunc 44 ...
-
bioRxiv - Microbiology 2021Quote: ... A TaqMan-based real-time PCR was then performed on the DNA samples using the QuantStudio 3 Real-Time PCR Systems (ThermoFisher Scientific, Waltham, MA, USA), using optical grade 96-well plates ...
-
bioRxiv - Molecular Biology 2022Quote: ... The copy number of each standard DNA was calculated based on the concentration quantified with the Qubit 3 Fluorometer (Thermo Fisher Scientific, MA, USA). Finally ...
-
bioRxiv - Molecular Biology 2023Quote: ... and transfected in suspension with 1 µg of p2Lox-based plasmid pML156 (Table S6) mixed with 3 µl of Lipofectamine 2000 (Thermo Fisher Scientific, cat# 11668019) and 100 µl of Opti-MEM I (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2020Quote: ... or the resazurin-based PrestoBlue assay (ThermoFisher). For both assays ...
-
bioRxiv - Microbiology 2021Quote: Custom-made pantothenate depleted medium was purchased from Gibco based on regular DMEM recipe (Gibco 41965039). 5% dialyzed FBS (Pan Biotech P30-2102 ...
-
bioRxiv - Microbiology 2020Quote: ... TaqMan-based Open Array panel (Thermo Fisher) and an additional SARS-CoV-2 RT-PCR research assay ...
-
bioRxiv - Developmental Biology 2022Quote: ... on column-based DNase I (ThermoFisher Scientific) digestion was performed following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... based qRT-PCR (Applied Biosystems StepOne Plus). The dilution at which no copies were detected was set as zero reference ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: TaqMan-based real-time PCR (Applied Biosystems) was also performed in a Step One Plus Detection System (Applied-Biosystems) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Enzyme-based chemiluminescence substrate (Thermo Fisher Scientific) was used to detect antibody-reactive protein bands.
-
bioRxiv - Neuroscience 2020Quote: ... The lysates were then used for quantification of soluble ATP molecules using luciferase-based commercial ATP quantification kit (ATP Determination kit, Thermo Fisher, Cat.no. A22066) with the help of a standard curve ...
-
bioRxiv - Bioengineering 2024Quote: The viability of dynamically and statically cultured cerebral organoids on day 31 was measured with the CellTiter-Glo 3D® cell viability assay kit as well as a fluorescence-based Live/Dead™ viability/cytotoxicity kit (L3224, Life Technologies) containing calcein AM and ethidium homodimer-1 (EthD-1 ...
-
bioRxiv - Microbiology 2023Quote: ... and 0.2 μl reverse primers (5 μM) in 96-well plates (MicroAmp Fast 96-well Reaction Plate, Applied Biosystems for Life Technologies). The reactions were performed in triplicates on a QuantStudio 5 Real-Time PCR System (Thermo Fischer Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were then washed in PBST-2 (PBS containing 0.1% Triton X-100) for 3 × 2 minutes and blocked with SeaBlock blocking buffer (ThermoFisher) for 15 minutes at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... braziliensis (MHOM/BR/1975/M2903) soluble antigen (2 μg/well) were adsorbed in microtiter plate (Nunc-immuno Plate, Roskilde, Denmark). Then ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... using SequalPrep™ Normalization Plate Kits (Thermo Fisher Scientific), and pooled for a purification step performed using Agencourt ® AMPure ® XP magnetic beads to size select PCR products for sequencing ...
-
bioRxiv - Molecular Biology 2021Quote: ... and stored at −80°C until DNA extraction with a magnetic-based method (MagMAX CORE extraction kit – ThermoFisher).
-
bioRxiv - Synthetic Biology 2022Quote: ... we first tried magnetic beads-based method using the MagJET plasmid DNA Kit (K2792) (ThermoFisher Scientific, Waltham, MA) on the Fluent ...
-
bioRxiv - Genomics 2021Quote: ... Residual RNA from PCR-based testing was reverse-transcribed using SuperScript™ VILO™ cDNA Synthesis Kit (ThermoFisher). NGS was performed using primer sets that tiled the entire SARS-CoV-2 genome (Ion AmpliSeq SARS-CoV-2 Research Panel ...
-
bioRxiv - Microbiology 2020Quote: ... while aliquots for RNA-based analyses were DNase-treated using the TURBO DNA-free™ kit (Thermo Fisher), followed by reverse transcription of the RNA to cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher ...
-
bioRxiv - Neuroscience 2021Quote: ... the relative ATP concentration of isolated neurons was measured using the bioluminescence-based ATP Determination Kit (Invitrogen, A22066) following the manufacturer’s recommended protocol ...
-
bioRxiv - Molecular Biology 2021Quote: 1 μg 18S IVT was polyadenylated with a E-PAP based Poly(A) Tailing Kit (Thermo Fisher Scientific) according to the manufacturers instructions and purified using RNA Clean and Concentrator kit (Zymo Research).
-
bioRxiv - Cell Biology 2023Quote: ... After a brief culture to expand the erythroblast population a Sendai virus based approach (CytoTune 2.0 Kit, Invitrogen) was used to express the Yamanaka factors without any genomic integration (27) ...
-
bioRxiv - Biochemistry 2023Quote: ... a differential scanning fluorimetry assay based on the use of SYPRO orange (Thermoshift assay kit, Thermo Fisher Scientific) was used to minimize protein consumption (Huynh & Partch ...
-
bioRxiv - Genetics 2023Quote: ... based on DNA concentration determined by Qubit fluorometer and Quant-iT dsDNA Assay kit (Life technologies, Paisley, UK) from a random subset of samples ...
-
bioRxiv - Cancer Biology 2023Quote: ... fluorescence-based quantification was performed using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, Waltham, MA, USA).
-
bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
-
bioRxiv - Cancer Biology 2020Quote: ... n = 3) or oligopyridylamides (5 µM ADH-1 or ADH-6, n = 3) using a combination of both TriZol (Thermo Fisher Scientific) and RNAeasy Mini Kit (Qiagen ...
-
bioRxiv - Microbiology 2021Quote: ... the hypervariable V3 region of the 16S rDNA gene was amplified from 20 ng of DNA using the primers 5′-CCTACGGGAGGCAGCAG-3′ and 5′-ATTACCGCGGCTGCTGG-3′ (Integrated DNA Technologies BVBA, Leuven, Belgium),(18) 1U Platinum® PCR SuperMix High Fidelity (ThermoFisher Scientific) and 10 μM of primer-mix ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were incubated in 0.05 M Tris-HCl buffer (pH 7.6) containing 5 mg 3-3′ diaminobenzidine (Thermo Scientific, TA-060-HDX) per 10 mL buffer and a final concentration of 0.01% hydrogen peroxide for 15 min at RT ...
-
bioRxiv - Immunology 2022Quote: ... and hybridized first using RT primer (5′/5Biosg/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3′) and then reverse transcribed into cDNA using TSO primer (5′-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3’) and RT maxima reverse transcription (Thermo Fisher Scientific). cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′ ...