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Citations for Thermo Fisher :
9401 - 9450 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Quantitative PCR (qPCR) was performed on a StepOne™ Real-Time PCR System (Applied Biosystems, Themo Fisher Scientific, CAT# 4376598) using Maxima SYBR Green qPCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 2μl of genomic DNA was added to a 25μl PCR reaction containing Phusion U Green Multiplex PCR Master Mix (Thermo Fisher) and 0.5μM of each forward and reverse primer for each target site ...
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bioRxiv - Cell Biology 2023Quote: ... This was used as a polymerase chain reaction (PCR) template to amplify and produce [Platinum SuperFi PCR master mix (Invitrogen)] the NaTrxhΔNα(+3 ...
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bioRxiv - Bioengineering 2023Quote: ... Real-time qRT-PCR was performed on an Applied Biosystems 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, USA) using Hieff qPCR SYBR Green Master Mix (Yeasen ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR was performed on generated cDNA with SYBR Green using QuantStudio 6 Real-Time PCR (ThermoFisher Scientific, Australia). Forward and reverse prime sequences for each gene of interest are shown in Table 7 ...
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bioRxiv - Physiology 2023Quote: qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems) on an Applied Biosystems 7900HT real-time PCR machine in 96-well-plate format ...
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bioRxiv - Neuroscience 2023Quote: ... All qRT-PCR analyses were performed on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). Target mRNA expression was normalised for RNA loading using Gapdh (Integrated DNA Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR (qPCRQpcr) was performed in order to analyse gene expression using Power SYBR Green PCR Master Mix (Applied Biosystems) instructions and different oligonucleotides (the sequences are available upon request) ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using the SYBR Green SuperMix and StepOne Software v.2.3 (Applied Biosystems) with 40 cycles of amplification (including three biological replicates) ...
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bioRxiv - Neuroscience 2023Quote: ... All qRT-PCR analyses were performed on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). Target mRNA expression was normalised for RNA loading using Gapdh (Integrated DNA Technologies) ...
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bioRxiv - Cell Biology 2023Quote: A PCR product encoding mouse RAB18 was subcloned into an intermediate TOPO vector using a TOPO PCR Cloning Kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting PCR product was cloned into the pCR 4-TOPO vector using the TOPO TA Cloning Kit for Sequencing (Invitrogen). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA samples were subjected to qRT-PCR quantification in duplicate and performed with Power SYBR Green PCR Master Mix (Invitrogen) according to the product guides on a Agilent Mx3005P machine.
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bioRxiv - Cell Biology 2023Quote: ... and PCR amplicons were cloned into the plasmid of the Zero Blunt TOPO PCR cloning kit (450031, Thermo Fisher Scientific) and sequenced ...
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bioRxiv - Genomics 2023Quote: ... 10-fold diluted cDNA was used for quantitative Real-time PCR with Power SYBR Green PCR Master Mix (4367659, Invitrogen) master mix with 500nM forward and reverse primer pairs (Supplementary table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR with reverse transcription (qRT-PCR) assays were performed using PowerUp SYBR Green Master Mix (ThermoFisher Scientific Cat# A25742) on the StepOne Real-Time PCR System (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The PCR products containing positive clones were purified using the ExoSAP-IT ™ PCR Product Cleanup Reagent (#78201 Applied Biosystems) and purified samples were sent for Sanger sequencing to Source Bioscience ...
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bioRxiv - Genetics 2023Quote: ... The donor template for homologous recombination was generated by PCR amplification of a human DNA sample carrying 51 CAG repeat into the pCR-Blunt II TOPO plasmid (Invitrogen). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... Resulting cDNA was used for quantitative PCR (qRT-PCR) using the TaqMan™ Gene Expression Master Mix (Applied Biosystems, #4369016). A list of all human TaqMan probes used is provided in Supplementary Table 5 ...
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bioRxiv - Cell Biology 2023Quote: ... and used for q-PCR in a Viia7 Real-time PCR thermal cycler using both Fast SYBR Green Master Mix (ThermoFisher), after standard curve method optimization to reach the 100% primer efficiency for each couple of primers listed in Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and used as a template in the PCR reactions performed with PCR Master Mix (2X) (K0172, Thermofisher). Primers 5’-AGGGTCTGACCATCATGTCC-3’ and 5’-GTGCTGTTCATCCACAATGC-3’ were used to detect the KO allele ...
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bioRxiv - Physiology 2023Quote: ... The qRT-PCR was carried out using the Applied Biosystems QuantStudio3 real-time PCR machine (Thermo Fisher, Waltham, MA, USA). For data analysis ...
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bioRxiv - Physiology 2024Quote: ... TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR) were then performed using a QuantStudio 7 Pro Real-Time PCR System (ThermoFisher). The housekeeping gene Vinculin was used as an internal control for cDNA quantification and normalization of gene amplified products.
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative real-time PCR was performed on the 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with 2 x SuperFast Universal SYBR Master Mix (CWBIO ...
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bioRxiv - Microbiology 2023Quote: ... and quantitative PCR (qPCR) was performed with Thunderbird Next SYBR qPCR mix (Toyobo #QPX-201) and StepOnePlus real-time PCR system (ThermoFisher) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... The relative levels of mRNA expression were examined utilizing real-time PCR (Applied Biosystems ViiA 7 Real-Time PCR System) with SYBR® GreenER™ reagent (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: Blunt-end topoisomerase-based cloning was performed using the PCR products (Zero Blunt TOPO PCR Cloning Kit (ThermoFisher Scientific #K2875J10)) ...
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bioRxiv - Cell Biology 2024Quote: ... the synthesized cDNA was subjected to real-time PCR analysis using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Genomics 2024Quote: ... of ebony were prepared by PCR for a final volume of 25 µL containing 1x Platinum II Hot-Start Green PCR Master Mix (Invitrogen), 0.2 µM of each forward and reverse primer (Supplementary Table 7) ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was purified using a Qiagen PCR purification kit and then phosphorylated using an Anza PNK kit (Invitrogen). Phosphorylation reactions were inactivated by incubating at 80°C for 5 min ...
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bioRxiv - Plant Biology 2024Quote: ... Two ul of each PCR product were used in the ligation reaction into pCR™-Blunt cloning vector (Invitrogen™) and Sanger sequenced for polymorphisms analysis.
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bioRxiv - Neuroscience 2024Quote: ... The Applied Biosystems 7500 FAST Real-Time PCR System or QuantStudio7 Flex Real-Time PCR system (Thermo Fisher Scientific Inc.) was used for quantitative PCR assay ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was analyzed for gene expression with quantitative PCR using a Quant Studio 3 real-time PCR system (Applied Biosystems). Forward and reverse primers for each gene analyzed are listed in Supplementary Table 4 ...
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bioRxiv - Immunology 2024Quote: ... and real-time PCR was performed with PowerUp SYBR Green PCR Master Mix in QuantStudio 5 system (Thermo Fisher Scientific). Relative fold change was calculated with 2−ΔΔCt methods ...
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bioRxiv - Neuroscience 2024Quote: ... The quantitative real-time polymerase chain reaction (PCR) was conducted using a Fast SYBR Green PCR Master Mix (Applied Biosystems) along with specific primers for Oxtr and GAPDH genes (HyLabs Israel Ltd.) ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... All PCR analyses were performed on DNA isolated from ear biopsies using Phire Tissue Master Mix PCR reagent (Thermo Fisher). The mice were kept in an animal facility with 12-hour light and 12-hour dark cycle and unrestricted access to other mice ...
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bioRxiv - Molecular Biology 2024Quote: ... The real-time quantitative PCR assays were carried out on the PikoReal 96 Real-Time PCR System (Thermo Fisher Scientific). Primers were designed using the Primer Quest program (http://eu.idtdna.com/PrimerQuest/Home/Index) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 30 mosquitoes were pooled in sets of 10 females and RNA was extracted using the Arcturus PicoPure RNA Isolation Kit (Life Technologies Carlsbad, California, United States). cDNA were synthesised from each RNA pool using the Superscript III (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... and surface markers in PBS for 30 min at 4°C before fixation and permeabilization using eBioscience FOXP3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, 00-5523-00) and staining for intracellular proteins ...
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bioRxiv - Immunology 2020Quote: ... then stained with surface and/or intracellular flow cytometry antibodies specific to antigens of interest using eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Waltham, MA) according to manufacturer protocol ...
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bioRxiv - Genetics 2022Quote: ... Sodium deoxycholate was removed by acidification with 10 % TFA and the sets were desalted using Pierce peptide desalting spin columns (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Gene expression data for GP130 (IL6ST) were extracted from the following data sets using the Oncomine™ Research Premium Edition database (Thermo Fisher, Ann Arbor, MI)36 ...
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bioRxiv - Bioengineering 2024Quote: A separate set of ATA samples (n=4 per group) were immersed in optimal cutting temperature compound (OCT; 4585, Thermo Fisher Scientific; Waltham, MA, USA) immediately after excision and frozen at 80◦C for fluorescent immunostaining ...
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bioRxiv - Neuroscience 2023Quote: ... which specifically targets on the phosphorylated Thr188 of murine C/EBPβ (a conservative locus to Thr235 of human C/EBPβ) in eBioscience™ Transcription Factor Staining Buffer Set (Invitrogen, Catalog #00-5523-00). For background signal staining ...
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bioRxiv - Immunology 2023Quote: ... CTOT01 and healthy donor cocultures were fixed and permeabilized following manufacturer protocol using eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (ThermoFisher, cat #00-5523-00). For CTOT19 coculture ...
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bioRxiv - Plant Biology 2020Quote: ... using two-step attB adaptor PCR (Life Technologies) with Phusion High Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Plant Biology 2020Quote: ... SYBR Green PCR master mix (Thermo Fisher Scientific) and gene-specific oligonucleotides (Key Resources Table ...
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bioRxiv - Synthetic Biology 2021Quote: ... in StepOne Real-Time PCR system (Applied Biosystems) with B-actin as housekeeping standard ...
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bioRxiv - Cancer Biology 2021Quote: ... Power SYBR Green PCR Master Mix (ThermoFisher. #4367659) was used ...
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bioRxiv - Cell Biology 2019Quote: ... and a StepOne plus PCR system (Applied Biosystems). Data were analyzed using StepOne v2.3 software (Applied Biosystems ...