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Citations for Thermo Fisher :
9351 - 9400 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... All quantitative PCR were performed in triplicate on an ABI 7500 Fast real-time PCR system (Applied Biosystems, Carlsbad, USA) using TransStart Tip Green qPCR SuperMix (Transgen ...
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bioRxiv - Genomics 2020Quote: ... All primers used in this work were purchased from Integrated DNA Technologies and PCRS were carried out using either Phusion U DNA Polymerase Green MultiPlex PCR Master Mix (ThermoFisher) or Q5 Hot Start High-Fidelity 2x Master Mix (New England Biolabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were purified using ExoSAP-IT PCR Product Clean up (Exonuclease I - Shrimp Alkaline Phosphatase, Thermo Fisher Scientific Inc) and were either sent to Macrogen Inc ...
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bioRxiv - Immunology 2020Quote: ... 2 and 3 cDNA were amplified by real-time PCR using the SYBR Green PCR kit (Thermo Scientific, Braunschweig, Germany). The primer sequences used for the real-time PCR were listed in Table 1.
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems) on an Applied Biosystems 7900HT real-time PCR machine in 96-well-plate format ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR was performed using qPCRBIO SyGreen Blue Mix Lo-ROX (PCR biosystems) and the QuantStudio 5 Real Time PCR system (ThermoFisher). The data were analysed using QuantStudio Design and Analysis software (ThermoFisher) ...
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bioRxiv - Genomics 2020Quote: ... DNA was isolated from peripheral blood and amplified by PCR (HotStart II High Fidelity PCR Master Mix, Thermo Fisher Scientific), followed by Sanger sequencing (Eurofins Genomics) ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were cleaned from gel using NucleoSpin Gel and PCR cleanup (MN) and cloned in TOPO-TA plasmids (Invitrogen) according to manufacturer’s specifications ...
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bioRxiv - Immunology 2022Quote: ... The amplification reaction was monitored on the ViiA 7 Real-Time PCR System running on the QuantStudio Real Time PCR Software V1.3 (Thermo Fisher). The size of the respective amplicons was confirmed by 2% agarose gel and ethidium bromide staining ...
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bioRxiv - Developmental Biology 2022Quote: ... Fragments of genomic DNA flanking the expected deletion sites were amplified by PCR using Platinum SuperFi II PCR master mix (Invitrogen). They were then sequenced by Sanger sequencing to confirm the outcome of the genome modification procedure ...
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bioRxiv - Biochemistry 2022Quote: ... Quantitative PCR reactions were carried out using PowerUp SYBR-Green master mix using StepOne Real-Time PCR systems (Applied Biosystems). GAPDH mRNA levels were used for normalization ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative real-time PCR was carried out in triplicates on StepOnePlus Real-time PCR System (Thermo Fisher Scientific, Carlsbad, USA) using QuantiTect® SYBR® Green PCR Kit (QIAGEN GmbH ...
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bioRxiv - Cancer Biology 2022Quote: ... An estimated 25ng of cDNA in 20μl reaction volume was analyzed in triplicate by quantitative RT-PCR amplification on a Viia7 Real-time PCR applied biosystem (Life Technology) using SYBR Green assay (QuantiFast SYBR Green PCR Kit, cat#4385618 from ThermoFisher) according to manufacturer’s instructions and for 40 cycles ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed using SYBR Select Master Mix and QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) with the following parameter ...
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bioRxiv - Cancer Biology 2022Quote: ... qRT-PCR was performed using Taqman universal PCR mix and predesigned gene expression assays with best coverage from Applied Biosystems. The following primer sets were used in our study:
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bioRxiv - Cancer Biology 2022Quote: ... This was followed by quantitative real-time PCR using Power SYBR Green PCR Master Mix [Thermo Fisher Scientific (Applied Biosystems), Waltham ...
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bioRxiv - Genetics 2022Quote: ... Expression levels of genes was quantified using qRT-PCR and the QuantStudio™5 Real-Time PCR system (Thermo Fisher). qRT-PCR was performed using the indicated qPCR primers (Supplementary Table 11) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was used to perform RRT-PCR reactions in the ABI 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA). Each reaction contained 12.5 µL of kit-supplied 2X RT-PCR Master mix ...
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bioRxiv - Neuroscience 2022Quote: Real-time PCR was performed with a 1:5 dilution of cDNA using QuantStudio3 real-time PCR apparatus (Life Technologies) and TaqMan® reagents with predesigned assays for the target genes GAD65 (alias GAD2 ...
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bioRxiv - Microbiology 2022Quote: ... using either a QuantStudio™ 7 Flex Real-Time PCR System or a StepOnePlus Real-Time PCR System (Applied Biosystems). Individual standard curves were generated specifically for each system ...
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bioRxiv - Immunology 2022Quote: PCR products (1-2 ng per 100 base pairs) were treated with ExoSAP-IT PCR Product Cleanup Reagent (Life Technologies) at a ratio of 5:2 at 37°C for 4 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR reaction was carried out in 96-well plates using the 7500 Fast Real-Time PCR System (Applied Biosystems). A melting curve analysis was performed for each sample to verify PCR specificity ...
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bioRxiv - Cancer Biology 2024Quote: ... and qRT-PCR was performed using SYBR Green Master Mix on a StepOnePlus Real-time PCR system (Thermo Fisher Scientific). Gene expression was normalized to 18s rRNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The Q-PCR were performed using the StepOne real-time PCR system and following its standard amplification protocol (Thermo Scientific). Relative gene expression levels were quantified using the comparative CT method (2-ΔΔCT method or 2-ΔCT ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... cDNA samples were diluted 5-folds and PCR was performed with StepOne Plus Real-Time PCR System (PE Applied Biosystems). The FastStart Universal SYBR Green Master (Roche ...
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bioRxiv - Molecular Biology 2024Quote: Quantitative PCR was performed with The StepOne™ Real-Time PCR System or QuantStudio™ 3 System (Applied Biosystems™) using 5 μl diluted cDNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... which was then immediately placed into a numbered PCR reaction (Platinum™ Green Hot Start PCR 2X Master Mix-Invitrogen). The sex-specific banding pattern following gel electrophoresis was evaluated for each egg to determine its sex ...
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bioRxiv - Cell Biology 2024Quote: Quantitative real-time PCR analysis was performed by using a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Darmstadt, Germany). Target transcripts were analysed by TaqMan® Gene Expression Assays or self-designed primers and the respective Master Mix ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR was performed using the StepOnePlus Real-Time PCR detection system (Applied Biosystems, Foster City, CA, USA) and Platinum SYBR Green qPCR super Mix (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... in a QuantStudio 6 Flex Real-Time PCR System using QuantStudio Real-Time PCR Software (both Applied Biosystems, Waltham, MA). TaqMan probes used were ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA concentration was quantified using a NanoDrop One spectrophotometer (Thermo) and 1 ng of DNA was amplified by PCR using the AmpFℓSTR Identifiler Plus PCR Amplification Kit (Applied Biosystems) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were diluted 10x and qRT-PCR reactions were performed using a QuantStudio 3 Real-Time PCR System (Applied Biosystems). For each reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products derived from ChIP-DNA were quantified by real-time PCR using the Fast ABI 7500 instrument (Applied Biosystems). The reaction mixture contained 2 µl of ChIP or input DNA (diluted 1:10 to represent 1% of input DNA) ...
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bioRxiv - Neuroscience 2024Quote: ... was performed using the Applied Biosystems QuantStudio 7 Flex Real-Time PCR system and analysed with the QuantStudio Real-Time PCR Software (v1.7 Thermo Fisher). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR reactions (25 µl) were carried out using DreamTaq Hot Start Green PCR mix solution (Thermo Fisher Scientific, Waltham, MA), with 0.4 µM of each Dystrophin primer ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time quantitative PCR was performed on 1/10 dilution of cDNA using SYBR green PCR master mix (Fisher Scientific) and the primers listed in Appendix Table S1.
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was carried out on the Applied Biosystems 7500 Real Time PCR system using Power SYBRgreen PCR master mix (Invitrogen), 2 ng of 1:10 diluted cDNAs per reaction and the following cycling conditions ...
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bioRxiv - Genetics 2023Quote: ... The resulting reconstituted PCR fragment was cloned using the Zero Blunt® TOPO® PCR cloning kit (Invitrogen, Carlsbad CA) and subcloned into the p1 vector as a SpeI-NotI restriction fragment ...
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bioRxiv - Genomics 2023Quote: ... Transposed fragments were PCR amplified for a number of PCR cycles determined from one-third of the maximum fluorescence measured by quantitative PCR reaction with 1 × SYBR Green (Invitrogen), custom-designed primers [70] ...
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bioRxiv - Genetics 2023Quote: ... The cDNAs were used as templates for real-time PCR and ran on StepOnePlus real-time PCR system (Applied Biosystems) using SYBR Green as a detection reagent ...
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bioRxiv - Immunology 2023Quote: ... and amplified by PCR using SYBR® Green PCR Master Mix and ABI Prism 7900HT sequence detection system (Applied Biosystems). Primers used for qPCR analysis are listed in Table S2.
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bioRxiv - Genomics 2023Quote: ... or control wild-type tissue were PCR amplified using the diluted protocol of the Phire Animal Tissue Direct PCR Kit (ThermoFisher) and a pair of oligonucleotides (Forward ...
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bioRxiv - Immunology 2024Quote: ... qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems) on an Applied Biosystems 7900HT real-time PCR machine in 96-well-plate format ...
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bioRxiv - Synthetic Biology 2023Quote: ... eBlocks were amplified via polymerase chain reaction (PCR) with Phusion High-Fidelity PCR Master Mix (cat. no. F531L) from ThermoFisher Scientific and purified using Qiagen PCR clean-up kits (cat ...
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bioRxiv - Microbiology 2023Quote: ... The resulting cDNA was utilized as input for quantitative PCR analysis on a StepOnePlus Real Time PCR System (Applied Biosystems) using the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR reactions were performed on a QuantStudio 7 Flex PCR machine using PowerUp Sybr Green Master Mix (Applied Biosystems). Corresponding changes in the expression levels of selected genes were calculated using the ΔΔCt method relative to housekeeping genes ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was conducted with a QuantStudio real-time PCR system or an ABI Prism system (both from Applied Biosystems), with the following experimental settings ...
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bioRxiv - Plant Biology 2023Quote: ... The quantitative PCR reaction was performed using Power SYBR® Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2023Quote: ... Quantitative real-time PCR was performed on a CFX Connect Real-Time PCR Detection System (Thermo Fisher Scientific., MA, USA) using an SYBR Green PCR kit (TransGen Biotech ...
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bioRxiv - Biochemistry 2023Quote: ... Quantitative PCR (qPCR) was performed on a StepOne™ Real-Time PCR System (Applied Biosystems, Themo Fisher Scientific, CAT# 4376598) using Maxima SYBR Green qPCR Master Mix (Thermo Fisher Scientific ...